| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 72,892 | G→T | H12Q (CAC→CAA) | thiQ ← | thiamine ABC transporter ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 72,892 | 0 | G | T | 98.7% | 245.6 / ‑4.0 | 74 | H12Q (CAC→CAA) | thiQ | thiamine ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (35/38); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
GCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAGCACGCATGCAAAGGCCAGCGCCTGC > NC_000913/72648‑73133 | gCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTaa < 1:328623/251‑1 (MQ=255) gATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGcc > 1:322304/1‑251 (MQ=255) tCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGt < 2:164242/250‑1 (MQ=255) gCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAgggggtatt > 2:177651/1‑244 (MQ=255) cGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATAtca < 1:274140/251‑1 (MQ=255) cGACCGTCAGGTGACTGAACAGGTTGTTTTCCGGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCTGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATAtca < 1:664217/251‑1 (MQ=255) gTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATAtcagtcag < 2:337658/251‑1 (MQ=255) gtgacTGAACGGGTTGTTTTCCTGAATCAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGTACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGGTCAGCAGGGTACTTTTACCTG‑CGCCGCTGGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTa < 2:588556/247‑1 (MQ=255) tgacTGAACAGGTTGTTTTCCTGAAACAGCATCTACACCTGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAAtgttgg < 2:320787/219‑1 (MQ=255) tgacTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAAtgttgg > 1:320787/4‑222 (MQ=255) acTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAAc > 1:562290/2‑250 (MQ=255) ctgtaCAGGTTGTTTTCCTGAAACAGCATCGACACAGGACGGCGTGAAGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGCACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAACAGGCTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTACCCGGGATCTCCCGTGAAACACAAACTCATCGCCAAATGTTGGTAAAGCCGGGTGATATACGCAAGTTTTAACAt > 2:511308/5‑251 (MQ=255) gAACAGGTTTTTTTCCTGAAACAGCAGCGACACCGGACGGCGTGACGGCGGCGGCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACAtc < 2:391171/250‑2 (MQ=255) ggTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGAGTGATCACCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCCTCGGCAAATGTTGGTAAAGCCCAGTGGTATCAGTCAGTTTTAACATTTCGccc > 2:425865/1‑251 (MQ=255) ggTTGTTGTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGccc < 1:291200/251‑1 (MQ=255) gttgttTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGcccc < 1:44122/251‑1 (MQ=255) tttcCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTa < 1:207164/248‑1 (MQ=255) ttcCTGAAACATCATCGACACCGGACGGAGTGACGGCGGGGTCGTGGTGTGAACAACGCCGTCGATAACCAGCGATCCGCTGGCTGGCGCCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGttg < 2:524112/249‑3 (MQ=255) ttcCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAg < 1:83965/249‑1 (MQ=255) ccAGCATCGACACAGGACGGCGTGCCGGCTGCGTCGTGGTGTGGTCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGAACAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTTAGCTAAAACGCATCGGTCAATGTTGGTAAAGCCAGGAGCTATCAGTCAGTTTTAACCTTTCGCCCCGGTAATTTTTccac > 2:301675/2‑248 (MQ=255) aCAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAAt > 1:699840/1‑251 (MQ=255) cAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGAAATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATc > 1:234547/1‑251 (MQ=255) aGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAATAAGCGAACCGCTGGCTGGCGTCAGAAAACAAGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGATCGACGCGTTCTACCGTTAAGCTAAAACGCATCGGCAACTGTTGGTAAAGACAGGTGATATCAGTCAGGTTTAACATCTCGCCCCGGTAGTTTTTCAAta > 1:703972/1‑249 (MQ=255) atcgaacccgtccGGCGTGACGGCGCGATCGTGGTGTGATCAAGGCCGTCGATAGTCAGCGAGCCGCGGGCTGGCGTCATAAACCCGTCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCGTGCCGCGTGCTACCGTTAAGCGAAAACGCAGCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTGTTCAATCACgg < 2:491846/239‑1 (MQ=255) cccGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAAca < 1:530579/250‑1 (MQ=255) ccGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAAcag < 2:454654/251‑1 (MQ=255) ccGGACGGCGTGACGGCGGCGTCGTGGTGTGAGCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTGCAATCACGGTAAAcag < 1:219768/251‑1 (MQ=255) aCGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAAcagc > 1:17259/1‑248 (MQ=255) cgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGCTCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCCCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagcagc > 2:366371/5‑248 (MQ=255) gtgtgATCAACGCCGTCGATAGTCAGCGCACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAAtt > 2:220108/1‑250 (MQ=255) gtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAACACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACCGAGTAGCAGCAGAAtt > 2:140136/1‑250 (MQ=255) gtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCCGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAAtt > 1:653924/1‑250 (MQ=255) tgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATAGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTa > 1:469040/1‑250 (MQ=255) gtgATCAACGACGTCGATAGTCAGCGAACCGATGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCGCGGTAGTGTTTCAATCACGGTAAACAGTAGAAAACAGAGTAGCAGCAGAATTAAc < 1:237695/251‑1 (MQ=255) caaCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGt < 1:513750/249‑1 (MQ=255) caaCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTGACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGt < 2:83218/249‑1 (MQ=255) aaCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTg > 1:702875/2‑251 (MQ=255) acgccggcGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGa < 1:563089/244‑1 (MQ=255) aTAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGt > 1:30549/1‑251 (MQ=255) aGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAAtgttgg < 2:209714/139‑1 (MQ=255) aGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAAtgttgg > 1:209714/1‑139 (MQ=255) gTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCt < 1:486554/251‑1 (MQ=255) gCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTTCTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCt < 2:653924/251‑1 (MQ=255) cGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGAt > 1:67130/1‑250 (MQ=255) gggctggcGGCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTGCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGTCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCTGTCCTGGCTGCGATAGGa < 1:156338/250‑1 (MQ=255) ggctggcGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAg < 1:73713/251‑1 (MQ=255) tCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTTCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCGCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAtt < 1:309548/249‑1 (MQ=255) gAAAACCGGCTATCAAGTTCAGCAGGGTACTTG‑ACCCGGCGCCGCTTGGCCCGAGGATCGCTACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGTTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTg < 2:26406/251‑1 (MQ=255) aCCGGCGAGCAAGTTACGCAGGGTACTTTTCCCTG‑CGCCGCTTGGCCCGAGGATCTCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAGTTGCTGGTag < 2:93357/251‑1 (MQ=255) gATCAAGTTCAGAAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCAACTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACAGCGCCGTACTGGCTGCGTTAGGAGCCAATTTGCTGGTAGAGATa > 1:219478/1‑250 (MQ=255) agttCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGa > 1:365887/3‑219 (MQ=255) agttCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGa < 2:365887/217‑1 (MQ=255) gttCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACACGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGCGATAAAACGGc > 1:232299/2‑251 (MQ=255) ttCAGCGGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGGGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGc > 2:600197/1‑250 (MQ=255) tCAGCAGGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGc > 1:628535/1‑249 (MQ=255) gcagGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGt < 1:274904/251‑1 (MQ=255) gcagGGTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGt < 1:425865/251‑1 (MQ=255) gggTACTTTTACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCg < 2:30549/251‑1 (MQ=255) ttttACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGt < 1:344441/223‑1 (MQ=255) ttttACCTG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGGGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGt > 2:344441/1‑223 (MQ=255) ttttACCTG‑CGCCGCTTGGCACGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGAGCGGAAatc > 1:564100/1‑251 (MQ=255) cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTAcc > 1:142364/1‑251 (MQ=255) ccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAACACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCg > 1:429390/1‑250 (MQ=255) cgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTACGCTAAAACGCATCGGAAAATGTTGGAAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGAGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATCAAACGGCAGGGTGCGGAAATCATCGTTACCGa > 1:609750/1‑250 (MQ=255) cgccgcGTTCTACCGGTAAGCTAAAACGCAACGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTGTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTGGCTGGTAGAGATAAAACGGCAGGGTGCGGAAAGCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑Ca < 2:322304/249‑1 (MQ=255) gccgcGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTCGCAGCAGAATTAACGCGGTGACCGCGACGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGAGGAAATCATCGTTACCGAAC‑AACGCCACCCCACCAAAATCAC‑AAATc > 1:453709/1‑251 (MQ=255) cgTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTAGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAAGCAC‑CAATCGAc > 1:413406/1‑250 (MQ=255) gTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCACCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGTAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCTGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAg < 1:42736/251‑1 (MQ=255) gTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCCCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAg < 2:447914/251‑1 (MQ=255) ctaCCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTa > 2:415417/3‑79 (MQ=255) ctaCCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTa < 1:415417/77‑1 (MQ=255) aCCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAGCa < 1:621366/248‑1 (MQ=255) ttAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCCCCACGCCAAAATCAC‑CAATCCACAGCACGCaggc > 2:295334/1‑248 (MQ=255) ttAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAGCACGCaggc > 1:301157/1‑248 (MQ=255) cTAAAACGCATCGGCCAATGTTGGTAAATCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAACAGAATTAACGCGGTGACCGCGCCGTCCTGGCCGCGAAAGGAGCCAATTTGCTGGTAGAGCTAAAACGGAAGGGTGCGGCAATCATCGTTACCTACCCAAC‑CCACCCCGCCAAAAT‑ACACAAACGACAACACGCA‑GCaaaaa > 2:356494/1‑248 (MQ=255) cATCGGCAAATGTTGGTACAGCCAGGTGATATCAGTCCGTTTTAACCTCTCGACCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAGCACGCCAGCAAAAACCAGCGcc > 2:324342/1‑251 (MQ=255) cGGCAAATGTTTGTAAATCCAGGTGAAATCAGGCAGTTGTAACATCGCGCCCCGGTAGTTTTTCAAGCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAGTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACAACGCCAAAATCCC‑CAATCGACAGCACGCAGGCAAAAGCCAGCGCCTgc < 2:653675/251‑1 (MQ=255) cGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAGCACGCAGGCAAAAGCCAGCGCCTg < 1:602166/250‑1 (MQ=255) | GCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCG‑CGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAAC‑AACGCCACCACGCCAAAATCAC‑CAATCGACAGCACGCATGCAAAGGCCAGCGCCTGC > NC_000913/72648‑73133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |