Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,794	T→C	A329A (GCA→GCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,794	0	T	C	94.3%	152.5 / 0.5	55	A329A (GCA→GCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (22/28);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-01

CGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATT  >  NC_000913/64558‑65033                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCCGGTTATAAGTCGCCAGCGCAGGttt                                                                                                                                                                                                                                      >  1:114403/1‑251 (MQ=255)    gAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATc                                                                                                                                                                                                                                   <  1:502053/251‑1 (MQ=255)       cccATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTc                                                                                                                                                                                                                                <  2:271167/250‑1 (MQ=255)        ccaacACGTAGGCGCCAGGGCGGGCGTGTGGCGGCTCTTCGCCGTGATTAGTCGCGACATACACAGCGCGGGGCCTACGAGGAAAATAGAGATGACCAAACGCCTCCACCGATCCCCCGTGGCGGTCGAGCGGCAGGCCTTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGTTTATCTTCg                                                                                                                                                                                                                               <  2:547186/247‑1 (MQ=255)          aTCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑Gc                                                                                                                                                                                                                            >  1:425597/1‑251 (MQ=255)            cACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGa                                                                                                                                                                                                                          <  1:4777/251‑1 (MQ=255)            cACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCg                                                                                                                                                                                                                           >  1:211800/1‑250 (MQ=255)                       cAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAGTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCa                                                                                                                                                                                                               <  2:425597/251‑1 (MQ=255)                        aGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCaa                                                                                                                                                                                                              <  1:617974/251‑1 (MQ=255)                          ggcgggcgagtggcggcACTTCGCCGAGATTAGGCTCGACATAACCAGAGCGGGGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCTATACTCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCGCGCAATCTTTTAGGTTATAAGTCGCTAGCGCAGGTTTATCTTCG‑GCGAAACGTCGGTCAA‑tt                                                                                                                                                                                                           <  2:253924/240‑1 (MQ=255)                          ggCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑tt                                                                                                                                                                                                           >  1:137699/1‑251 (MQ=255)                               gCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGGTTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTAGCCAGCGCAGGTTTATCTTCGAGC‑AAACGGCGGGCAA‑ttttttc                                                                                                                                                                                                      >  2:170983/1‑247 (MQ=255)                                       cggcACTTCGCCGAGATTAGGCGCGACTTAACCAGCGCGGTGCATACGAGGAAAATAGAGATGGCCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGAt                                                                                                                                                                                              <  2:470418/251‑1 (MQ=255)                                                 ccGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCCTTCGATCCC‑ACggggt                                                                                                                                                                                   >  1:212743/1‑249 (MQ=255)                                                      aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCgt                                                                                                                                                                              >  1:364266/1‑250 (MQ=255)                                                      aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTGCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGa                                                                                                                                                                              <  1:339844/251‑1 (MQ=255)                                                       ttAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTAACGAt                                                                                                                                                                             >  1:650974/1‑251 (MQ=255)                                                             gcgACAGAACCAGCGCTGTGCATACGCGGAAAATAGAGATGACCAAACGCCGCCTCCGATCCCCCGTGGCGGGCTACCGGCAGGCCGTTCACCGTCGATCGTTCGAGTAAAAATGGCTGGAGTTCAGTTTTATGGAAAAGCTGCGTCCCCAGCGCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑GTGCGTCCATTCGATCCC‑GCGGGTTATCGATAGAttt                                                                                                                                                                       <  2:619079/251‑1 (MQ=255)                                                              cgACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGGCAAATTTCG‑CCCTTCGTTCCC‑CCGGGGTATCGAGAGAtttt                                                                                                                                                                      >  2:13574/1‑251 (MQ=255)                                                               gACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGAttttt                                                                                                                                                                     >  1:118258/1‑250 (MQ=255)                                                                aCATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTcc                                                                                                                                                                    <  1:512754/251‑1 (MQ=255)                                                                                  atacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑CCGGGTTATCGATAGATTTTCCTTCGCCtcattacttctt                                                                                                                                                  >  1:306315/7‑240 (MQ=255)                                                                                  atacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTg                                                                                                                                                  <  1:701523/245‑1 (MQ=255)                                                                                    acgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGGTAGATTTTCCTTCCCCTAATAACTCCTggg                                                                                                                                                >  1:80321/5‑249 (MQ=255)                                                                                       ggaaaaaTAGAAATGCCCAAGTGCCGCCCCGGAACCGCCGGGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGa                                                                                                                                             <  2:211800/248‑1 (MQ=255)                                                                                       aggcaaaTAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGCTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAAAGGCGGTCAA‑ATTCTTCCCATCGCTCCCCACGG‑TGATCGGTAGAATTTCCTTCGCCTAATacatcctgggcg                                                                                                                                              >  2:507344/5‑239 (MQ=255)                                                                                        ggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAc                                                                                                                                            <  1:162730/251‑1 (MQ=255)                                                                                         gAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcg                                                                                                                                           <  1:656035/251‑2 (MQ=255)                                                                                              tAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTTTAGCTAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTc                                                                                                                                      >  1:113001/1‑251 (MQ=255)                                                                                                 agaggaccaatctccgccACCGATCCTCCGTGGCGGTTTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAAGCTGCGTCACCAGCTCGCAATCTCTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCG‑GCGAAACGGCGGTCAA‑GTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGAGTTTCCTACGCCTAATAACTCCTGAGCGACGGTTTCCAg                                                                                                                                   <  2:76227/238‑1 (MQ=255)                                                                                                     gACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATc                                                                                                                                                                                >  1:199210/1‑202 (MQ=255)                                                                                                     gACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATc                                                                                                                                                                                <  2:199210/202‑1 (MQ=255)                                                                                                      aCCAAACGCCGCCACCGATCCCCCGTGGCGTTCTACCGGCAGGCCGTTTACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCGTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATGCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCgaga                                                                                                                              <  1:444914/251‑1 (MQ=255)                                                                                                         aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGGCGGGTTCCAGCGGGAATg                                                                                                                           >  1:403346/5‑251 (MQ=255)                                                                                                         aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATg                                                                                                                           <  2:306315/247‑1 (MQ=255)                                                                                                                       atcccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGa                                                                                                                                                                                               <  1:348722/165‑1 (MQ=255)                                                                                                                                  cGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCATCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGAttt                                                                                                  <  2:260320/251‑1 (MQ=255)                                                                                                                                       cACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGTTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                                                             <  1:157314/251‑1 (MQ=255)                                                                                                                                        aCCGGCAGGCCGTTCACCGTCGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTAAGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                                                             <  2:330005/250‑1 (MQ=255)                                                                                                                                           ggCAGGCCGTTCTCCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGTTTTATCTTCG‑GCGAAACGGCTGTCAA‑TTTCGTCCAGTCGTTCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGTGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                                                         <  1:688701/251‑1 (MQ=255)                                                                                                                                           ggCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGCTTTATGGAAAATCTGCGTCACCAGCTCGCAATCATTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTGCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACGCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                                                         <  2:118258/251‑1 (MQ=255)                                                                                                                                                       aCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGAt                                                                             <  1:13574/251‑1 (MQ=255)                                                                                                                                                              cgcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTGTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCTATAATTa                                                                        <  2:650974/247‑1 (MQ=255)                                                                                                                                                                    cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTGCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCt                                                                <  1:65577/251‑1 (MQ=255)                                                                                                                                                                                     aTTTCAGTTTTCTGGAAAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATAAGTCGCCAGAGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGCTCCC‑ACGGGTTATCTATAGATTTTCCTTCGCCTAATCACTCCTGCGCGACGGGTTCCAGCGAGAATGAAGAGAAAATCCCGAAAGCGTATTTCAGCGCCTCGATACCGTCGATAATTATACGCCATTTGGCCTTGGCaaaaa                                                >  2:78215/1‑250 (MQ=255)                                                                                                                                                                                      tttCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCCA‑TTTTGGCCCTTCGATTCC‑ACGGGTTATCTATAGATTTTTCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGGGTATGAATAGAAATTCCAGAACCCGGATTTCAACACCTCGATACCTCCGCTAATTCGTCGGCCTTTAGACTGGGCaaacaa                                               >  1:269291/1‑247 (MQ=255)                                                                                                                                                                                             tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGGAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGt                                       >  1:702365/5‑251 (MQ=255)                                                                                                                                                                                             tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGTAAAAAAGACGCCg                                        <  1:555262/246‑1 (MQ=255)                                                                                                                                                                                                  ggAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGGTAATTTGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGgtttt                                   >  2:466846/1‑250 (MQ=255)                                                                                                                                                                                                    aaaaTCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTaa                                 >  1:434528/4‑250 (MQ=255)                                                                                                                                                                                                         cggCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCACGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGt                           <  2:124074/249‑1 (MQ=255)                                                                                                                                                                                                            cGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCCTTCGATCCC‑CCGGGTTATCGATAGATTTTCCTTCTCCTAATAACTCCTGTGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCCGCGCCTCGATACCTTCGATAATTAGCCGACCTTTAGCCTGGGCAAACAAGTCGCCGTTTTTAAAGCCGTGCt                        >  2:126111/1‑251 (MQ=255)                                                                                                                                                                                                              tcaccagccagaaaTCTTTCAGGTTATCAGTAGCCAGAGCCGGTTTATATTCG‑GCGAAACGGCGGACAA‑CTTAGTCCATTCGATCCC‑ACGGGTTAACCATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAAGGTTTCCCGCGCGGATGAAGAGAAATTCCAGCACCCGGATTTCCGCGCCCCGATCCCGACGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCAGTTTTTAAAGCCgcgcctg                      >  2:352892/13‑245 (MQ=255)                                                                                                                                                                                                                                tCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCCTTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGGAGAGAAATTCCAGAACGCGGGTTTCAGCGCCTCGATACCGTCGGTAATTATCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGGGATCGCGCCACTCCACCTcactg    >  2:263346/1‑247 (MQ=255)                                                                                                                                                                                                                                   ggTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTcactgtt  >  1:146901/1‑244 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   CGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATT  >  NC_000913/64558‑65033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑