Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 72,517 T→G R137R (CGA→CGC)  thiQ ← thiamine ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091372,5170TG96.9% 275.2 / ‑3.9 99R137R (CGA→CGC) thiQthiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base G (40/53);  total (43/56)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 111 total aligned reads displayed.

GCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGC  >  NC_000913/72271‑72763
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
gCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑t                                                                                                                                                                                                                                                    >  1:377045/1‑251 (MQ=255)
  tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgt                                                                                                                                                                                                                                                  >  1:473237/1‑251 (MQ=255)
  ccaacacatcctggctcttACCCTGGCTGGCGATGCGCCCGTCGGCGACCACCACCGAGCGTGTGGCGAGCCGCGCGGCATCTTCCACACCGTTTGACACCATCTATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGATAACGGTTCATCGAGCAATAAAATCGGCTGGTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tg                                                                                                                                                                                                                                                   <  2:380863/232‑1 (MQ=255)
     acaacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgc                                                                                                                                                                                                                                               >  1:449623/1‑251 (MQ=255)
      caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAATTCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgct                                                                                                                                                                                                                                              >  1:256999/1‑251 (MQ=255)
      caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACCGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgct                                                                                                                                                                                                                                              >  2:146009/1‑251 (MQ=255)
       cacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACCCCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACTTAACGCCGGATCGAGCGCAGAGAAAGGTTCATCGAGCAATAAAATCGGCTGTTCCCGAACAAG‑ACAACGCGGTAACGCCACgcca‑tgtcgctg                                                                                                                                                                                                                                             >  2:401387/2‑251 (MQ=255)
       aacgcATTGGTTGTACCCGGCCAGGCGAGGCGCCCGTCGGCGACCGCCACCGAGCGCGTGGCGATCCGCGCGGCATCGTCCACACTGTGGGACACAATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCGGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg                                                                                                                                                                                                                                             <  2:126087/247‑1 (MQ=255)
        acTCATTGGTCTTACCCTGCCAGGCGAGGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑CCAACGCGCTAACGCACCgcgc‑tgtcgctgg                                                                                                                                                                                                                                            >  2:344071/1‑250 (MQ=255)
                gtcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGa                                                                                                                                                                                                                                    <  2:377045/247‑1 (MQ=255)
                 tcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGCAAAAC‑CGCTAACGCCCCCCCC‑TGTCGCTGCCCCCCGGa                                                                                                                                                                                                                                    >  2:433216/4‑250 (MQ=255)
                   ttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg                                                                                                                                                                                                                                             >  1:320021/2‑239 (MQ=255)
                    ttcccTGCCAGGCGATGTGCCCGTCGGCGACCACCCCCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGATACCATCAATATCGTCATTTTTTGCTGCTGGCAGCTCGCGCTCACCAGCGTTAGCACCTCCTGACGTAACGCCGGACCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGTTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAg                                                                                                                                                                                                                                 <  1:258520/248‑1 (MQ=255)
                    tACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGaaa                                                                                                                                                                                                                                  <  1:533882/249‑1 (MQ=255)
                    tACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCAGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGaaa                                                                                                                                                                                                                                  <  1:189778/249‑1 (MQ=255)
                     aCCCTGCCAGGCGATGCGCCCGTCGGCGCCCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGCCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTAATCGAGCAATAAAATCGGCTGTTAGCGCACCGG‑ACAACGCTCTACCGCCACACGCCTG‑CGCTGACCGCCGGAAAggc                                                                                                                                                                                                                               >  2:317465/1‑249 (MQ=255)
                            cAGGCGATGCGCCAGTCGGCGAACACCACCGAGAGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGACGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACAGCCGGAAAGCTCGccagg                                                                                                                                                                                                                        >  2:316903/1‑248 (MQ=255)
                                 gATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑CCAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAAcc                                                                                                                                                                                                                   >  2:505551/1‑251 (MQ=255)
                                  aTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCACTAAAATCGGCTGTTCGCGCACCCG‑ACAACCCGCTAACGCCACGCCC‑TGTACCTGACCGCCGGaaatcccgccagggaaccc                                                                                                                                                                                                                  >  2:399025/1‑235 (MQ=255)
                                    gcgcCCGCCGGCGACCACCACCGAGCGCGGGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACcgcg                                                                                                                                                                                                                <  1:490892/251‑1 (MQ=255)
                                         cGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt                                                                                                                                                                                                           <  1:348239/251‑1 (MQ=255)
                                         cGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt                                                                                                                                                                                                           >  2:100214/1‑251 (MQ=255)
                                          gTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGccctt                                                                                                                                                                                                           >  2:677608/1‑247 (MQ=255)
                                             ggCGACCACCACCGCGCGCGTGGCGATCCGCGCGGCATCTGCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGCTCGAGCGCAGAGACCGGTTCATCGAGCAATAACATCGGCTGTTCGCACACCAG‑ACCACGCGCTCACGCCACCCGC‑TGTAGCTGACCTCCGGAAAGCTCGCCAGAGAACCGCGcccttcat                                                                                                                                                                                                        >  2:453347/1‑244 (MQ=255)
                                             ggCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACCCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAAt                                                                                                                                                                                                       >  2:498405/1‑251 (MQ=255)
                                                gaccaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTAt                                                                                                                                                                                                    >  1:311618/8‑251 (MQ=255)
                                                 accaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGa                                                                                                                                                                                                                                            <  1:687268/204‑1 (MQ=255)
                                                       acCGAGCGCGTGTCGAGCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATccc                                                                                                                                                                                             <  2:449623/251‑1 (MQ=255)
                                                       acCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGCTCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCCG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAAGTAACCGCGCCCTTAAATTATCAATccc                                                                                                                                                                                             >  2:367126/1‑251 (MQ=255)
                                                           aGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATc                                                                                                                                                                                         >  1:433131/1‑251 (MQ=255)
                                                            gcgcGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATACCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCt                                                                                                                                                                                        <  1:118723/251‑1 (MQ=255)
                                                                gTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCTGAAAGCGCGACAGGCAACCGCGCCATTAAATTATCAATCCCCATCTggcg                                                                                                                                                                                    >  2:15894/1‑251 (MQ=255)
                                                                      aTCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGAt                                                                                                                                                                              <  1:433216/251‑1 (MQ=255)
                                                                       cccccaccgcATCTGCCACGCGGGGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGCACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTATCGCCGCTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTggcgggcgtta                                                                                                                                                                             <  2:23102/245‑4 (MQ=255)
                                                                        ccGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGAt                                                                                                                                                                              >  2:336952/1‑249 (MQ=255)
                                                                         cgcgcgGCATCTTCCTCACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCCCCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGc                                                                                                                                                                           <  1:270353/251‑1 (MQ=255)
                                                                           cgcggcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGCTAGCGt                                                                                                                                                                         >  1:369299/5‑251 (MQ=255)
                                                                              ggcATCTTCCACACTGGGTGACACCATCAATAACGTCATTTTTTGCGGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCa                                                                                                                                                                      <  1:614622/250‑1 (MQ=255)
                                                                               gcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACAAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAAGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATAAACTTATCAATCCCCATCTGGCGGGCGATAGCGTGCAt                                                                                                                                                                     >  1:377407/1‑251 (MQ=255)
                                                                                cATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAtt                                                                                                                                                                    >  2:530579/1‑251 (MQ=255)
                                                                                     tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct                                                                                                                                                                <  1:100214/250‑2 (MQ=255)
                                                                                     tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct                                                                                                                                                                <  1:485996/250‑2 (MQ=255)
                                                                                     tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct                                                                                                                                                                <  1:149741/250‑2 (MQ=255)
                                                                                      ccACCCTGTGTGACACCATCAATAACTTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTctcc                                                                                                                                                              <  2:253871/251‑4 (MQ=255)
                                                                                      ccACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTctcc                                                                                                                                                              >  2:607253/1‑248 (MQ=255)
                                                                                          ccTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGGGCTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgct                                                                                                                                                          <  2:92005/250‑1 (MQ=255)
                                                                                          acTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgct                                                                                                                                                          <  1:272069/250‑1 (MQ=255)
                                                                                               gtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGtgcc                                                                                                                                                     <  1:366056/249‑4 (MQ=255)
                                                                                                      caccAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCTACGTCAGCATCTCCTGACGTAACGCCGGATCGAGCTCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCa                                                                                                                                              <  2:552869/248‑1 (MQ=255)
                                                                                                      cATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCAt                                                                                                                                                                                          <  1:9753/207‑1 (MQ=255)
                                                                                                       aTCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAg                                                                                                                                             <  1:422700/251‑1 (MQ=255)
                                                                                                          aacaacGTCATTTTTGGCTGCTGGCAGCTCGTTCTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGttt                                                                                                                                          <  2:207465/246‑1 (MQ=255)
                                                                                                            taacGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTc                                                                                                                                         >  1:140477/4‑250 (MQ=255)
                                                                                                             accGTCATTTTGTGGTGTTGGCAGCTCGTGCTCACCAACGTCAGCATCGCCTGACGGAACGACGGATCGGGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGTTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCAGCTGGCGGGCGATAGCGTGCATTTGCTCCTGCTGTGCCGCGTTCAGTTTCa                                                                                                                                        <  2:288289/248‑1 (MQ=255)
                                                                                                                            ggctgGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGGTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAAGCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGccc                                                                                                                        <  1:663638/250‑1 (MQ=255)
                                                                                                                                  cAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGAGCCATTAAATTATCAATCCCCATCTTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGGTCAGTTTCAATCCCGGATTTAGACCCAGccc                                                                                                                  >  2:177329/1‑251 (MQ=255)
                                                                                                                                   aGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCg                                                                                                                 >  1:669622/1‑251 (MQ=255)
                                                                                                                                       cGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATg                                                                                                              <  1:707109/250‑1 (MQ=255)
                                                                                                                                        gggctccccagcgacaggaTCTCCAGACTTAACGCCGGATCGAGCGCAGAGACCGGTCCACCGAGCAATAAAACCGGCTGTTCGCGCACCAG‑ACAACGCGTTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGGTTCAATCCCGGATTTAGCCCCAGCCCGATGtt                                                                                                            <  2:664746/233‑1 (MQ=255)
                                                                                                                                        gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGCGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTACCTTATCAATCCCCCTCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCCGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt                                                                                                            >  2:291200/1‑251 (MQ=255)
                                                                                                                                        gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGACCGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt                                                                                                            >  1:244066/1‑251 (MQ=255)
                                                                                                                                        gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt                                                                                                            >  1:134053/1‑251 (MQ=255)
                                                                                                                                             cACCAGCGTCAGCATCTCCGTACGTACCGCCGGATCGGGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCGTACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGGGCATTTTCTCCGGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTcggc                                                                                                        <  2:374851/250‑4 (MQ=255)
                                                                                                                                                      cagcacctcctaccGTAACGCCGGACCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCGGTTCGGGCACAAG‑ACAACGCGCTAACGTCACGCGC‑TGTCGCGGACCGCCGGAAAGCTCGCCAGGTACCCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTc                                                                                               <  2:256999/237‑1 (MQ=255)
                                                                                                                                                              ccTGACGTAACGCCGGTTCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTg                                                                                      >  1:524112/1‑251 (MQ=255)
                                                                                                                                                                     gaaCGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGCTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACGGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAAACCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGgt                                                                               <  2:33074/250‑1 (MQ=255)
                                                                                                                                                                       actccgcaTCGAGCGCAAAGAACGGGTCACCGGCCAATAAAATCGGCCGTCCGCGCACAAA‑AAACCGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAGTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGgttg                                                                             <  2:246578/244‑1 (MQ=255)
                                                                                                                                                                                cGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTg                                                                     <  1:453347/250‑1 (MQ=255)
                                                                                                                                                                                     gcAGAGAACGGTTCAGCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAg                                                               <  1:677608/251‑1 (MQ=255)
                                                                                                                                                                                          gaACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGGTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCg                                                          <  1:697199/251‑1 (MQ=255)
                                                                                                                                                                                             cGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGac                                                        >  1:83218/1‑250 (MQ=255)
                                                                                                                                                                                                ttCATCGAGCAATAAAATCGGCTGGTCCCGCACCAG‑ACAACGCGCGAACGCCGCGCGT‑TGTCGCTGCCCGCCGGAAAGCTCGCCAGGAAACCGCGCCAGTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAAGCCCGGATTTAGCCCCAGCCCGATGTTCTGCTCGACCGTCAGGTGACTGAACAGGTTGTTTCCCTGAACCAGCATCGACAcc                                                     <  2:215659/250‑1 (MQ=255)
                                                                                                                                                                                                     cGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACgg                                               <  1:15894/251‑1 (MQ=255)
                                                                                                                                                                                                          aaTAAAATCGGCTGCACGCACACCAG‑ACAACGCGCTAACGCCAAGCGC‑TGTCGCTGCACGCCGGAAAGCTCGCCAGGTATCCGCGCCATTAAATTATCAATCCCCATCTTGCGGGAGATAGAGTGCATTTTCTCCTGCTGGGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCAACCCAGAAGTTCTGCGCGACCGTCAGGTGGCTGCACAGGTATTTTACCTGTAACAGCATCggaaacggaaggcgtgc                                          >  2:606357/1‑235 (MQ=255)
                                                                                                                                                                                                                  cGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAttt                                                                                                                                                                   >  2:501551/1‑122 (MQ=255)
                                                                                                                                                                                                                  cGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAttt                                                                                                                                                                   <  1:501551/122‑1 (MQ=255)
                                                                                                                                                                                                                      tGTTCGCGCCCCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTc                                                                                                                                                                 <  1:466253/120‑1 (MQ=255)
                                                                                                                                                                                                                      tGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGAGGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCTGGACggcggcgtcgg                              >  1:20449/1‑246 (MQ=255)
                                                                                                                                                                                                                      tGTTCGAGCACCAG‑ACAACTCGCTAACGCCACGCGC‑TGTCGCTGACCGTCGGACAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGAGCAtttta                                                                                                                                                                 >  2:466253/1‑119 (MQ=255)
                                                                                                                                                                                                                       gTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcgcgatcggaat                              >  2:150737/1‑240 (MQ=255)
                                                                                                                                                                                                                       gTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcg                                        <  1:150737/240‑1 (MQ=255)
                                                                                                                                                                                                                        ttCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtgg                            >  1:116682/1‑244 (MQ=255)
                                                                                                                                                                                                                            cgcaCCAG‑ACACCGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtggtgtg                        <  2:116682/248‑12 (MQ=255)
                                                                                                                                                                                                                              caCCAG‑ACAACGTGCTAGCGCCACTCGC‑TTTCGCTTACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAACTATCAATCCCCATCCTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTTCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcggcgtcgtcgtggtgtgat                      <  2:446767/250‑16 (MQ=255)
                                                                                                                                                                                                                                     aCAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGGGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGCCGGCGTGCCGGCGGCGTCGTGGTGTGATCAACGc                >  2:161604/1‑251 (MQ=255)
                                                                                                                                                                                                                                         cgtggtAACGCCACGAGC‑TGTCGTTACCCGCCGGAAAGCTCGCCAGGTAACCGGGCCATTTAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCGCCTTCTGTGCCGCGGTCAGTTTCAATCCCGGATTTAGCTCCAGCCCGATGTTCTGCGCGACCGTCAGTTGACTGAACAGGTTGTTTTCCTGAAACAGCATAGACCCCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccggc            <  2:538745/246‑4 (MQ=255)
                                                                                                                                                                                                                                         cgcgCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgtc            >  1:93357/1‑248 (MQ=255)
                                                                                                                                                                                                                                         cgcgCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGccgtc            >  1:648462/1‑248 (MQ=255)
                                                                                                                                                                                                                                          gcgcTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAGTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAAAAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCg           <  1:78702/251‑1 (MQ=255)
                                                                                                                                                                                                                                          cccgtAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAACTCCGCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCg           <  1:146009/247‑1 (MQ=255)
                                                                                                                                                                                                                                           cgcTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCg           >  1:454654/1‑250 (MQ=255)
                                                                                                                                                                                                                                            gcTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGAt         <  1:630387/251‑1 (MQ=255)
                                                                                                                                                                                                                                              tAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCg                                                    <  1:8071/206‑1 (MQ=255)
                                                                                                                                                                                                                                              tAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCg                                                    >  2:8071/1‑206 (MQ=255)
                                                                                                                                                                                                                                               aaCGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAg       <  1:389903/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCGCGCCAGGTAACCGCGCCATTAAATTATCAAGCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCa    <  2:134053/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGCCACCGGACGGCGTGACGGCGGCGTCGAGGTGTGATCAACGccgccggtagtccg   >  2:486554/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  gCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAg   <  1:336952/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAg   <  1:139350/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGc  <  1:399025/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
GCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGC  >  NC_000913/72271‑72763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑