| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 72,493 | T→C | V145V (GTA→GTG) | thiQ ← | thiamine ABC transporter ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 72,493 | 0 | T | C | 98.0% | 316.6 / ‑2.6 | 103 | V145V (GTA→GTG) | thiQ | thiamine ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (52/48); total (53/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-02 | |||||||||||
Only 100 of 103 total aligned reads displayed.
ATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTG > NC_000913/72246‑72737 | aTAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCAcc > 1:446767/1‑251 (MQ=255) aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGCTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGCCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc > 2:229188/1‑251 (MQ=255) aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc > 1:92005/1‑251 (MQ=255) aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc > 1:33074/1‑251 (MQ=255) tCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAc > 1:545725/1‑251 (MQ=255) cGCCTTACCGCTCACCAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAc < 1:663763/250‑1 (MQ=255) ccTTACCGCTCAACAACTCAGTGGTATTACCCTGTCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGCTCCGCGCGGCATCTCCCACACTGTGGGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGcc < 2:130820/251‑1 (MQ=255) tACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCAcg > 1:203184/1‑250 (MQ=255) tACCGCGCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCa < 2:290148/249‑1 (MQ=255) cGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGAGGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGCGACAGGTTCATCGAGCAATAAAATCGGCTGTTAGCGCACCAG‑ACACCGCGCTAACGccccgcac > 1:75190/1‑245 (MQ=255) gCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑t > 1:377045/1‑251 (MQ=255) tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCCTTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGCGCACTAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTACCGCCCCgcgc‑tgt > 2:209334/1‑251 (MQ=255) ccaacacatcctggctcttACCCTGGCTGGCGATGCGCCCGTCGGCGACCACCACCGAGCGTGTGGCGAGCCGCGCGGCATCTTCCACACCGTTTGACACCATCTATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGATAACGGTTCATCGAGCAATAAAATCGGCTGGTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tg < 2:380863/232‑1 (MQ=255) acaacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgc > 1:449623/1‑251 (MQ=255) caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAATTCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgct > 1:256999/1‑251 (MQ=255) caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACCGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgct > 2:146009/1‑251 (MQ=255) cacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACCCCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACTTAACGCCGGATCGAGCGCAGAGAAAGGTTCATCGAGCAATAAAATCGGCTGTTCCCGAACAAG‑ACAACGCGGTAACGCCACgcca‑tgtcgctg > 2:401387/2‑251 (MQ=255) aacgcATTGGTTGTACCCGGCCAGGCGAGGCGCCCGTCGGCGACCGCCACCGAGCGCGTGGCGATCCGCGCGGCATCGTCCACACTGTGGGACACAATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCGGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg < 2:126087/247‑1 (MQ=255) acTCATTGGTCTTACCCTGCCAGGCGAGGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑CCAACGCGCTAACGCACCgcgc‑tgtcgctgg > 2:344071/1‑250 (MQ=255) gtcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGa < 2:377045/247‑1 (MQ=255) tcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGCAAAAC‑CGCTAACGCCCCCCCC‑TGTCGCTGCCCCCCGGa > 2:433216/4‑250 (MQ=255) ttccccTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg < 2:320021/236‑1 (MQ=255) ttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg > 1:320021/2‑239 (MQ=255) ttcccTGCCAGGCGATGTGCCCGTCGGCGACCACCCCCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGATACCATCAATATCGTCATTTTTTGCTGCTGGCAGCTCGCGCTCACCAGCGTTAGCACCTCCTGACGTAACGCCGGACCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGTTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAg < 1:258520/248‑1 (MQ=255) tACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGaaa < 1:533882/249‑1 (MQ=255) tACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCAGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGaaa < 1:189778/249‑1 (MQ=255) aCCCTGCCAGGCGATGCGCCCGTCGGCGCCCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGCCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTAATCGAGCAATAAAATCGGCTGTTAGCGCACCGG‑ACAACGCTCTACCGCCACACGCCTG‑CGCTGACCGCCGGAAAggc > 2:317465/1‑249 (MQ=255) cAGGCGATGCGCCAGTCGGCGAACACCACCGAGAGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGACGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACAGCCGGAAAGCTCGccagg > 2:316903/1‑248 (MQ=255) gATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑CCAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAAcc > 2:505551/1‑251 (MQ=255) aTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCACTAAAATCGGCTGTTCGCGCACCCG‑ACAACCCGCTAACGCCACGCCC‑TGTACCTGACCGCCGGaaatcccgccagggaaccc > 2:399025/1‑235 (MQ=255) gcgcCCGCCGGCGACCACCACCGAGCGCGGGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACcgcg < 1:490892/251‑1 (MQ=255) cGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt < 1:348239/251‑1 (MQ=255) cGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt > 2:100214/1‑251 (MQ=255) gTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGccctt > 2:677608/1‑247 (MQ=255) ggCGACCACCACCGCGCGCGTGGCGATCCGCGCGGCATCTGCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGCTCGAGCGCAGAGACCGGTTCATCGAGCAATAACATCGGCTGTTCGCACACCAG‑ACCACGCGCTCACGCCACCCGC‑TGTAGCTGACCTCCGGAAAGCTCGCCAGAGAACCGCGcccttcat > 2:453347/1‑244 (MQ=255) ggCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACCCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAAt > 2:498405/1‑251 (MQ=255) gaccaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTAt > 1:311618/8‑251 (MQ=255) accaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGa < 1:687268/204‑1 (MQ=255) actacCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAAt < 1:325396/251‑1 (MQ=255) acCGAGCGCGTGTCGAGCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATccc < 2:449623/251‑1 (MQ=255) acCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGCTCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCCG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAAGTAACCGCGCCCTTAAATTATCAATccc > 2:367126/1‑251 (MQ=255) aGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATc > 1:433131/1‑251 (MQ=255) gcgcGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATACCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCt < 1:118723/251‑1 (MQ=255) gTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCTGAAAGCGCGACAGGCAACCGCGCCATTAAATTATCAATCCCCATCTggcg > 2:15894/1‑251 (MQ=255) gATCCGCGCGGCATCTTCCACACAGTGTGACACAATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCAAACGCCACGCGC‑TGTCGCTGACCGCCGGCAAACTACCCCGGTACACGCGCCATTAAATTAACAATCCCACTCTggcgggcg > 2:418436/1‑250 (MQ=255) aTCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGAt < 1:433216/251‑1 (MQ=255) cccccaccgcATCTGCCACGCGGGGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGCACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTATCGCCGCTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTggcgggcgtta < 2:23102/245‑4 (MQ=255) ccGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGAt > 2:336952/1‑249 (MQ=255) cgcgcgGCATCTTCCTCACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCCCCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGc < 1:270353/251‑1 (MQ=255) cgcggcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGCTAGCGt > 1:369299/5‑251 (MQ=255) ggcATCTTCCACTCTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGTCAGCTCGTGCTCACCAGCGTCAGCATCTCCTTACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCa < 1:49239/250‑1 (MQ=255) ggcATCTTCCACACTGGGTGACACCATCAATAACGTCATTTTTTGCGGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCa < 1:614622/250‑1 (MQ=255) gcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACAAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAAGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATAAACTTATCAATCCCCATCTGGCGGGCGATAGCGTGCAt > 1:377407/1‑251 (MQ=255) cATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAtt > 2:530579/1‑251 (MQ=255) aTCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGCGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAttt > 1:432114/1‑251 (MQ=255) ttCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGACTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCACCAGGTAACCGCGCCATTAAATTATCAATCCACATCTGGCGGGCGATAGCGTGCATTTTcc > 2:513750/1‑251 (MQ=255) tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct < 1:485996/250‑2 (MQ=255) tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct < 1:100214/250‑2 (MQ=255) tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct < 1:149741/250‑2 (MQ=255) ccACCCTGTGTGACACCATCAATAACTTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTctcc < 2:253871/251‑4 (MQ=255) ccACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTctcc > 2:607253/1‑248 (MQ=255) ccTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGGGCTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgct < 2:92005/250‑1 (MQ=255) acTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgct < 1:272069/250‑1 (MQ=255) gtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGtgcc < 1:366056/249‑4 (MQ=255) caccAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCTACGTCAGCATCTCCTGACGTAACGCCGGATCGAGCTCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCa < 2:552869/248‑1 (MQ=255) cATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCAt < 1:9753/207‑1 (MQ=255) aTCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAg < 1:422700/251‑1 (MQ=255) aacaacGTCATTTTTGGCTGCTGGCAGCTCGTTCTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGttt < 2:207465/246‑1 (MQ=255) taacGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTc > 1:140477/4‑250 (MQ=255) accGTCATTTTGTGGTGTTGGCAGCTCGTGCTCACCAACGTCAGCATCGCCTGACGGAACGACGGATCGGGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGTTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCAGCTGGCGGGCGATAGCGTGCATTTGCTCCTGCTGTGCCGCGTTCAGTTTCa < 2:288289/248‑1 (MQ=255) ggctgGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGGTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAAGCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGccc < 1:663638/250‑1 (MQ=255) cAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGAGCCATTAAATTATCAATCCCCATCTTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGGTCAGTTTCAATCCCGGATTTAGACCCAGccc > 2:177329/1‑251 (MQ=255) aGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCg > 1:669622/1‑251 (MQ=255) cGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATg < 1:707109/250‑1 (MQ=255) gggctccccagcgacaggaTCTCCAGACTTAACGCCGGATCGAGCGCAGAGACCGGTCCACCGAGCAATAAAACCGGCTGTTCGCGCACCAG‑ACAACGCGTTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGGTTCAATCCCGGATTTAGCCCCAGCCCGATGtt < 2:664746/233‑1 (MQ=255) gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGCGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTACCTTATCAATCCCCCTCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCCGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt > 2:291200/1‑251 (MQ=255) gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGACCGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt > 1:244066/1‑251 (MQ=255) gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt > 1:134053/1‑251 (MQ=255) cACCAGCGTCAGCATCTCCGTACGTACCGCCGGATCGGGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCGTACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGGGCATTTTCTCCGGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTcggc < 2:374851/250‑4 (MQ=255) cagcacctcctaccGTAACGCCGGACCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCGGTTCGGGCACAAG‑ACAACGCGCTAACGTCACGCGC‑TGTCGCGGACCGCCGGAAAGCTCGCCAGGTACCCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTc < 2:256999/237‑1 (MQ=255) ccTGACGTAACGCCGGTTCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTg > 1:524112/1‑251 (MQ=255) ccTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTg < 1:68298/251‑1 (MQ=255) aCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACa < 1:166643/251‑1 (MQ=255) gaaCGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGCTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACGGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAAACCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGgt < 2:33074/250‑1 (MQ=255) cGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTg < 1:453347/250‑1 (MQ=255) gcAGAGAACGGTTCAGCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAg < 1:677608/251‑1 (MQ=255) gaACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGGTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCg < 1:697199/251‑1 (MQ=255) cGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGac > 1:83218/1‑250 (MQ=255) ttCATCGAGCAATAAAATCGGCTGGTCCCGCACCAG‑ACAACGCGCGAACGCCGCGCGT‑TGTCGCTGCCCGCCGGAAAGCTCGCCAGGAAACCGCGCCAGTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAAGCCCGGATTTAGCCCCAGCCCGATGTTCTGCTCGACCGTCAGGTGACTGAACAGGTTGTTTCCCTGAACCAGCATCGACAcc < 2:215659/250‑1 (MQ=255) cGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACgg < 1:15894/251‑1 (MQ=255) gCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TATCGCTGACCGCCGGCCAGCTCGCCAGGTAACCACGCCATTAGATTATCAATCCCCATCTGGCGGGCGATATCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCCATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACGGGTTGTTTTCCTTCAACAGCCCCGCCCCCGGACGGCgg > 2:103855/1‑250 (MQ=255) aaTAAAATCGGCTGCACGCACACCAG‑ACAACGCGCTAACGCCAAGCGC‑TGTCGCTGCACGCCGGAAAGCTCGCCAGGTATCCGCGCCATTAAATTATCAATCCCCATCTTGCGGGAGATAGAGTGCATTTTCTCCTGCTGGGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCAACCCAGAAGTTCTGCGCGACCGTCAGGTGGCTGCACAGGTATTTTACCTGTAACAGCATCggaaacggaaggcgtgc > 2:606357/1‑235 (MQ=255) cGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAttt < 1:501551/122‑1 (MQ=255) cGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAttt > 2:501551/1‑122 (MQ=255) tGTTCGCGCCCCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTc < 1:466253/120‑1 (MQ=255) tGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGAGGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCTGGACggcggcgtcgg > 1:20449/1‑246 (MQ=255) tGTTCGAGCACCAG‑ACAACTCGCTAACGCCACGCGC‑TGTCGCTGACCGTCGGACAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGAGCAtttta > 2:466253/1‑119 (MQ=255) gTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcgcgatcggaat > 2:150737/1‑240 (MQ=255) gTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcg < 1:150737/240‑1 (MQ=255) ttCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtgg > 1:116682/1‑244 (MQ=255) | ATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTG > NC_000913/72246‑72737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |