BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,423	A→G	L169L (TTG→CTG)	thiQ ←	thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,423	0	A	G	95.7%	336.8 / 1.0	115	L169L (TTG→CTG)	thiQ	thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (68/42); total (70/45)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 115 total aligned reads displayed.

GCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGT  >  NC_000913/72178‑72661

gcactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCAtct                                                                                                                                                                                                                                            >  1:508501/8‑251 (MQ=255)

 cactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCCTCTTCCACACTGTGTGACACCATCCATAACGTCCTTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCAtctc                                                                                                                                                                                                                                           >  2:44122/7‑251 (MQ=255)

    tgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCCTCAATAACGCAATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCACCTg                                                                                                                                                                                                                                        >  2:149741/4‑251 (MQ=255)

    tACCCGTAGGTCAGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTg                                                                                                                                                                                                                                        >  1:55711/1‑251 (MQ=255)

     gtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCt                                                                                                                                                                                                                                         <  1:144894/247‑1 (MQ=255)

     gtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCt                                                                                                                                                                                                                                         <  1:228813/247‑1 (MQ=255)

     gtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTg                                                                                                                                                                                                                                        >  1:664746/3‑250 (MQ=255)

       ccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACg                                                                                                                                                                                                                                     >  2:522392/1‑251 (MQ=255)

        cGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGt                                                                                                                                                                                                                                    >  1:290148/1‑251 (MQ=255)

         gTAGGTCGGATAAGGTGTTCACGCCGAATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCTGCGACCACCACCGAGCGCTTGGCGATCCGCGCGGCATCTTCCTCACTGTGTGCCACATTCCATAACGTCCTTTTTTGCTACTGGCAGCTC‑‑TGTCTCCCCAGCGTCAGCATCTCCTGACGt                                                                                                                                                                                                                                    >  2:630387/1‑250 (MQ=255)

          tAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTa                                                                                                                                                                                                                                   >  1:215659/1‑250 (MQ=255)

            ggTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACg                                                                                                                                                                                                                                >  1:588556/1‑251 (MQ=255)

                ggATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCCGCATCTCCTGACGTAACGcagg                                                                                                                                                                                                                            >  2:485785/1‑248 (MQ=255)

                  atcaggtgTTCACGCCGCCTCTGACCGTGCATACTAACCCGTAATACCCAATAGCGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACACTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGAGTGGCGATCCGCGCGGCCTCTTCCACACTGTGGAACACCATCAATAACTTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCATCAGCATCAGCATCTCCTGACGCAACGCCGGac                                                                                                                                                                                                                          >  2:219768/8‑250 (MQ=255)

                  aTAAGGCTTTCACGCCGCACCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGAt                                                                                                                                                                                                                          <  2:659108/251‑1 (MQ=255)

                     aggtgTTCACGCCGCATCTGACAGTGCATACTAACCAGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCCCCAGCGTCAGCATCTCCTGACGTACCGCCGGATCg                                                                                                                                                                                                                        >  2:389903/5‑250 (MQ=255)

                       gtgTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTGACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAgc                                                                                                                                                                                                                     <  1:515805/249‑1 (MQ=255)

                               gccgcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAgaga                                                                                                                                                                                                              >  1:77195/1‑250 (MQ=255)

                                  gcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACg                                                                                                                                                                                                           <  1:236088/250‑1 (MQ=255)

                                          cAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCACAGTCAGAATCCCCTGACGTAACGCCGGATCAAGCGAAGCGAACGTTTCATCg                                                                                                                                                                                                   >  1:491846/1‑250 (MQ=255)

                                           aGTGCATACTAACCCGTACTCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGAAGAAAACGGTTCATCgcg                                                                                                                                                                                                 >  2:68298/1‑249 (MQ=255)

                                              gCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCa                                                                                                                                                                                               <  1:146419/250‑1 (MQ=255)

                                                aTACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATa                                                                                                                                                                                            <  1:207359/251‑1 (MQ=255)

                                                     aaCCCGTAATCCCAAATAGAGCTGAAGCACTCGCCTTACCGCTCACCAACTCCTTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGCCCACCACCGAGCGCGCGGCGATCCGCGCGGCATCTTCCACACTGTGTGCCACCATCAATACCGTCATTTTTTGCTGCTTGCAG‑TCAGTG‑CTCACCCGCGTCAGCATCTCCTGACGTCACGCCGCATCGCGCGCAgagacagcttcatctagcactcaaatc                                                                                                                                                                                       >  2:207164/1‑228 (MQ=255)

                                                       cccGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTAATCGAGCAATacattcgg                                                                                                                                                                                     >  2:707109/1‑244 (MQ=255)

                                                             aTCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTc                                                                                                                                                                               >  2:272069/1‑251 (MQ=255)

                                                                cccAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCACTAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcc                                                                                                                                                                            >  2:166643/1‑250 (MQ=255)

                                                                cccAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCAAACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGAATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg                                                                                                                                                                            >  1:348263/1‑251 (MQ=255)

                                                                    atagggctgAAGCACTAGCCTTACCGCTCAACAACTCATAGGTCTTACCATGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACAACATCAATACCGTCATTTTTTGCTGATGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCAGACGTAACGCCGGATCGAGCGCAGAGAACGGTACATCGAGCAATAAAATCGGCTGTTcgcgcccc                                                                                                                                                                        >  2:22573/9‑247 (MQ=255)

                                                                    aTAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCAcc                                                                                                                                                                        >  1:446767/1‑251 (MQ=255)

                                                                       gTGCTGAAGCACACGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCCGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGAATCTTCCACACTGTGTGACACAATCAAAAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CGCACCAGCATCAACATCTCCTGACGTAACGCCGGATCGATGGCAGCAACCGGTTCATCGAACAATAGAATCGGCtatgctcgcaca                                                                                                                                                                        >  2:274140/1‑237 (MQ=255)

                                                                             aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGCTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGCCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc                                                                                                                                                              >  2:229188/1‑251 (MQ=255)

                                                                             aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc                                                                                                                                                              >  1:92005/1‑251 (MQ=255)

                                                                             aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc                                                                                                                                                              >  1:33074/1‑251 (MQ=255)

                                                                                   tCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAc                                                                                                                                                        >  1:545725/1‑251 (MQ=255)

                                                                                    cGCCTTACCGCTCACCAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAc                                                                                                                                                        <  1:663763/250‑1 (MQ=255)

                                                                                      ccTTACCGCTCAACAACTCAGTGGTATTACCCTGTCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGCTCCGCGCGGCATCTCCCACACTGTGGGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGcc                                                                                                                                                     <  2:130820/251‑1 (MQ=255)

                                                                                         tACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCAcg                                                                                                                                                  >  1:203184/1‑250 (MQ=255)

                                                                                         tACCGCGCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCa                                                                                                                                                    <  2:290148/249‑1 (MQ=255)

                                                                                            cGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGAGGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGCGACAGGTTCATCGAGCAATAAAATCGGCTGTTAGCGCACCAG‑ACACCGCGCTAACGccccgcac                                                                                                                                               >  1:75190/1‑245 (MQ=255)

                                                                                             gCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgct                                                                                                                                              >  1:377045/1‑251 (MQ=255)

                                                                                               tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCCTTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGCGCACTAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTACCGCCCCgcgctgt                                                                                                                                            >  2:209334/1‑251 (MQ=255)

                                                                                               tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgctgt                                                                                                                                            >  1:473237/1‑251 (MQ=255)

                                                                                                  acaacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgctgtcgc                                                                                                                                         >  1:449623/1‑251 (MQ=255)

                                                                                                   caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACCGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgctgtcgct                                                                                                                                        >  2:146009/1‑251 (MQ=255)

                                                                                                    cacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACCCCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACTTAACGCCGGATCGAGCGCAGAGAAAGGTTCATCGAGCAATAAAATCGGCTGTTCCCGAACAAG‑ACAACGCGGTAACGCCACgccatgtcgctg                                                                                                                                       >  2:401387/2‑251 (MQ=255)

                                                                                                    aacgcATTGGTTGTACCCGGCCAGGCGAGGCGCCCGTCGGCGACCGCCACCGAGCGCGTGGCGATCCGCGCGGCATCGTCCACACTGTGGGACACAATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCGGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgctgtcgctg                                                                                                                                       <  2:126087/247‑1 (MQ=255)

                                                                                                     acTCATTGGTCTTACCCTGCCAGGCGAGGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑CCAACGCGCTAACGCACCgcgctgtcgctgg                                                                                                                                      >  2:344071/1‑250 (MQ=255)

                                                                                                             gtcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGa                                                                                                                              <  2:377045/247‑1 (MQ=255)

                                                                                                              tcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGCAAAAC‑CGCTAACGCCCCCCCCTGTCGCTGCCCCCCGGa                                                                                                                              >  2:433216/4‑250 (MQ=255)

                                                                                                                ttccccTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgctgtcgctg                                                                                                                                       <  2:320021/236‑1 (MQ=255)

                                                                                                                ttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgctgtcgctg                                                                                                                                       >  1:320021/2‑239 (MQ=255)

                                                                                                                 ttcccTGCCAGGCGATGTGCCCGTCGGCGACCACCCCCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGATACCATCAATATCGTCATTTTTTGCTGCTGGCAGCTC‑GCG‑CTCACCAGCGTTAGCACCTCCTGACGTAACGCCGGACCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGTTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAg                                                                                                                           <  1:258520/248‑1 (MQ=255)

                                                                                                                 tACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCAGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGaaa                                                                                                                            <  1:189778/249‑1 (MQ=255)

                                                                                                                         cAGGCGATGCGCCAGTCGGCGAACACCACCGAGAGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGACGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACAGCCGGAAAGCTCGccagg                                                                                                                  >  2:316903/1‑248 (MQ=255)

                                                                                                                              gATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑CCAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAAcc                                                                                                             >  2:505551/1‑251 (MQ=255)

                                                                                                                               aTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCACTAAAATCGGCTGTTCGCGCACCCG‑ACAACCCGCTAACGCCACGCCCTGTACCTGACCGCCGGaaatcccgccagggaaccc                                                                                                            >  2:399025/1‑235 (MQ=255)

                                                                                                                                 gcgcCCGCCGGCGACCACCACCGAGCGCGGGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACcgcg                                                                                                          <  1:490892/251‑1 (MQ=255)

                                                                                                                                      cGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt                                                                                                     >  2:100214/1‑251 (MQ=255)

                                                                                                                                      cGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt                                                                                                     <  1:348239/251‑1 (MQ=255)

                                                                                                                                       gTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGccctt                                                                                                     >  2:677608/1‑247 (MQ=255)

                                                                                                                                          ggCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACCCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAAt                                                                                                 >  2:498405/1‑251 (MQ=255)

                                                                                                                                             gaccaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTAt                                                                                              >  1:311618/8‑251 (MQ=255)

                                                                                                                                              accaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGa                                                                                                                                      <  1:687268/204‑1 (MQ=255)

                                                                                                                                                    acCGAGCGCGTGTCGAGCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATccc                                                                                       <  2:449623/251‑1 (MQ=255)

                                                                                                                                                    acCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGCTCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCCG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAAGTAACCGCGCCCTTAAATTATCAATccc                                                                                       >  2:367126/1‑251 (MQ=255)

                                                                                                                                                        aGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATc                                                                                   >  1:433131/1‑251 (MQ=255)

                                                                                                                                                         gcgcGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATACCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCt                                                                                  <  1:118723/251‑1 (MQ=255)

                                                                                                                                                             gTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCTGAAAGCGCGACAGGCAACCGCGCCATTAAATTATCAATCCCCATCTggcg                                                                              >  2:15894/1‑251 (MQ=255)

                                                                                                                                                                  gATCCGCGCGGCATCTTCCACACAGTGTGACACAATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCAAACGCCACGCGCTGTCGCTGACCGCCGGCAAACTACCCCGGTACACGCGCCATTAAATTAACAATCCCACTCTggcgggcg                                                                          >  2:418436/1‑250 (MQ=255)

                                                                                                                                                                   aTCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGAt                                                                        <  1:433216/251‑1 (MQ=255)

                                                                                                                                                                    cccccaccgcATCTGCCACGCGGGGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGCACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTATCGCCGCTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTggcgggcgtta                                                                       <  2:23102/245‑4 (MQ=255)

                                                                                                                                                                     ccGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGAt                                                                        >  2:336952/1‑249 (MQ=255)

                                                                                                                                                                      cgcgcgGCATCTTCCTCACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCCCCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGc                                                                     <  1:270353/251‑1 (MQ=255)

                                                                                                                                                                        cgcggcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGCTAGCGt                                                                   >  1:369299/5‑251 (MQ=255)

                                                                                                                                                                           ggcATCTTCCACTCTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGTCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTTACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCa                                                                <  1:49239/250‑1 (MQ=255)

                                                                                                                                                                           ggcATCTTCCACACTGGGTGACACCATCAATAACGTCATTTTTTGCGGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCa                                                                <  1:614622/250‑1 (MQ=255)

                                                                                                                                                                            gcATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACAAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAAGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATAAACTTATCAATCCCCATCTGGCGGGCGATAGCGTGCAt                                                               >  1:377407/1‑251 (MQ=255)

                                                                                                                                                                             cATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAtt                                                              >  2:530579/1‑251 (MQ=255)

                                                                                                                                                                              aTCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGCGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCAttt                                                             >  1:432114/1‑251 (MQ=255)

                                                                                                                                                                                 ttCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGACTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCACCAGGTAACCGCGCCATTAAATTATCAATCCACATCTGGCGGGCGATAGCGTGCATTTTcc                                                          >  2:513750/1‑251 (MQ=255)

                                                                                                                                                                                  tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct                                                          <  1:485996/250‑2 (MQ=255)

                                                                                                                                                                                  tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct                                                          <  1:100214/250‑2 (MQ=255)

                                                                                                                                                                                  tCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTct                                                          <  1:149741/250‑2 (MQ=255)

                                                                                                                                                                                   ccACCCTGTGTGACACCATCAATAACTTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTctcc                                                        <  2:253871/251‑4 (MQ=255)

                                                                                                                                                                                       ccTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GGG‑CTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgct                                                    <  2:92005/250‑1 (MQ=255)

                                                                                                                                                                                       acTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgct                                                    <  1:272069/250‑1 (MQ=255)

                                                                                                                                                                                            gtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGtgcc                                               <  1:366056/249‑4 (MQ=255)

                                                                                                                                                                                                   caccAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCTACGTCAGCATCTCCTGACGTAACGCCGGATCGAGCTCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCa                                        <  2:552869/248‑1 (MQ=255)

                                                                                                                                                                                                   cATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCAt                                                                                    <  1:9753/207‑1 (MQ=255)

                                                                                                                                                                                                    aTCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAg                                       <  1:422700/251‑1 (MQ=255)

                                                                                                                                                                                                       aacaacGTCATTTTTGGCTGCTGGCAGCTC‑GTT‑CTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGttt                                    <  2:207465/246‑1 (MQ=255)

                                                                                                                                                                                                         taacGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTc                                   >  1:140477/4‑250 (MQ=255)

                                                                                                                                                                                                          accGTCATTTTGTGGTGTTGGCAGCTC‑GTG‑CTCACCAACGTCAGCATCGCCTGACGGAACGACGGATCGGGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGTTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCAGCTGGCGGGCGATAGCGTGCATTTGCTCCTGCTGTGCCGCGTTCAGTTTCa                                  <  2:288289/248‑1 (MQ=255)

                                                                                                                                                                                                                         ggctgGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGGTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAAGCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGccc                  <  1:663638/250‑1 (MQ=255)

                                                                                                                                                                                                                                    c‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATg        <  1:707109/250‑1 (MQ=255)

                                                                                                                                                                                                                                      gTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGCGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTACCTTATCAATCCCCCTCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCCGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt      >  2:291200/1‑251 (MQ=255)

                                                                                                                                                                                                                                      gTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGACCGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt      >  1:244066/1‑251 (MQ=255)

                                                                                                                                                                                                                                      gTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGtt      >  1:134053/1‑251 (MQ=255)

                                                                                                                                                                                                                                            cACCAGCGTCAGCATCTCCGTACGTACCGCCGGATCGGGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCGTACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGGGCATTTTCTCCGGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTcggc  <  2:374851/250‑4 (MQ=255)

GCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGT  >  NC_000913/72178‑72661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`