Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,755	G→A	I342I (ATC→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,755	0	G	A	94.8%	169.2 / 0.6	58	I342I (ATC→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (24/31);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

GGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTT  >  NC_000913/64514‑65000                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                         ggCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTc                                                                                                                                                                                                                                                 >  1:470418/1‑251 (MQ=255)    ttttATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCAcc                                                                                                                                                                                                                                              >  1:363444/1‑251 (MQ=255)         taGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATCCGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGATCACCGGCGCTCGTTCGGGTAAAAAAGGCCTTATTTCCGTTTTATTGAAAATCTGCGTCACCaacac                                                                                                                                                                                                                                         >  2:555262/1‑247 (MQ=255)          agtccggctccaggtccgAATAATAAAGCCCGGGCCGTGAATCCATCACGTAGCCGCCAGGGCTTGCGGGTGGGGGCACTACGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAACCTGCGTCACCAGCTCg                                                                                                                                                                                                                                        <  2:278578/234‑1 (MQ=255)                         ccgAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTta                                                                                                                                                                                                                         <  2:144743/249‑1 (MQ=255)                           gAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCCGGCCGTTCACCGTCCCCCGGTCGGGTAAAAATGGCCTGGTTTCAGTTTTTTGGAAAATCTTCGTCACCAACTCGCAATCTTTTCGGTtat                                                                                                                                                                                                                        >  2:162730/1‑250 (MQ=255)                           aaaTCATAAAGCCCTGGCCGGTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACGTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCTTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGATAATCTGCGTCACCAGCTCGCAATCTTTCAGGTtata                                                                                                                                                                                                                       <  2:330047/250‑1 (MQ=255)                                 tAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCATCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGtcgc                                                                                                                                                                                                                 >  1:563692/1‑248 (MQ=255)                                             cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCCGGTTATAAGTCGCCAGCGCAGGttt                                                                                                                                                                                                     >  1:114403/1‑251 (MQ=255)                                                gAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATc                                                                                                                                                                                                  <  1:502053/251‑1 (MQ=255)                                                   cccATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTc                                                                                                                                                                                               <  2:271167/250‑1 (MQ=255)                                                    ccaacACGTAGGCGCCAGGGCGGGCGTGTGGCGGCTCTTCGCCGTGATTAGTCGCGACATACACAGCGCGGGGCCTACGAGGAAAATAGAGATGACCAAACGCCTCCACCGATCCCCCGTGGCGGTCGAGCGGCAGGCCTTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGTTTATCTTCg                                                                                                                                                                                              <  2:547186/247‑1 (MQ=255)                                                      aTCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑Gc                                                                                                                                                                                           >  1:425597/1‑251 (MQ=255)                                                        cACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGa                                                                                                                                                                                         <  1:4777/251‑1 (MQ=255)                                                        cACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCg                                                                                                                                                                                          >  1:211800/1‑250 (MQ=255)                                                                   cAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAGTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCa                                                                                                                                                                              <  2:425597/251‑1 (MQ=255)                                                                    aGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCaa                                                                                                                                                                             <  1:617974/251‑1 (MQ=255)                                                                      ggcgggcgagtggcggcACTTCGCCGAGATTAGGCTCGACATAACCAGAGCGGGGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCTATACTCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCGCGCAATCTTTTAGGTTATAAGTCGCTAGCGCAGGTTTATCTTCG‑GCGAAACGTCGGTCAA‑tt                                                                                                                                                                          <  2:253924/240‑1 (MQ=255)                                                                      ggCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑tt                                                                                                                                                                          >  1:137699/1‑251 (MQ=255)                                                                           gCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGGTTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTAGCCAGCGCAGGTTTATCTTCGAGC‑AAACGGCGGGCAA‑ttttttc                                                                                                                                                                     >  2:170983/1‑247 (MQ=255)                                                                                   cggcACTTCGCCGAGATTAGGCGCGACTTAACCAGCGCGGTGCATACGAGGAAAATAGAGATGGCCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGAt                                                                                                                                                             <  2:470418/251‑1 (MQ=255)                                                                                             ccGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCCTTCGATCCC‑ACggggt                                                                                                                                                  >  1:212743/1‑249 (MQ=255)                                                                                              cGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGt                                                                                                                                                                                                                                                  <  1:567056/157‑1 (MQ=255)                                                                                                  aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCgt                                                                                                                                             >  1:364266/1‑250 (MQ=255)                                                                                                  aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTGCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGa                                                                                                                                             <  1:339844/251‑1 (MQ=255)                                                                                                   ttAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTAACGAt                                                                                                                                            >  1:650974/1‑251 (MQ=255)                                                                                                         gcgACAGAACCAGCGCTGTGCATACGCGGAAAATAGAGATGACCAAACGCCGCCTCCGATCCCCCGTGGCGGGCTACCGGCAGGCCGTTCACCGTCGATCGTTCGAGTAAAAATGGCTGGAGTTCAGTTTTATGGAAAAGCTGCGTCCCCAGCGCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑GTGCGTCCATTCGATCCC‑GCGGGTTATCGATAGAttt                                                                                                                                      <  2:619079/251‑1 (MQ=255)                                                                                                          cgACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGGCAAATTTCG‑CCCTTCGTTCCC‑CCGGGGTATCGAGAGAtttt                                                                                                                                     >  2:13574/1‑251 (MQ=255)                                                                                                           gACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGAttttt                                                                                                                                    >  1:118258/1‑250 (MQ=255)                                                                                                            aCATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTcc                                                                                                                                   <  1:512754/251‑1 (MQ=255)                                                                                                                              atacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑CCGGGTTATCGATAGATTTTCCTTCGCCtcattacttctt                                                                                                                 >  1:306315/7‑240 (MQ=255)                                                                                                                              atacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTg                                                                                                                 <  1:701523/245‑1 (MQ=255)                                                                                                                                acgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGGTAGATTTTCCTTCCCCTAATAACTCCTggg                                                                                                               >  1:80321/5‑249 (MQ=255)                                                                                                                                   ggaaaaaTAGAAATGCCCAAGTGCCGCCCCGGAACCGCCGGGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGa                                                                                                            <  2:211800/248‑1 (MQ=255)                                                                                                                                   aggcaaaTAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGCTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAAAGGCGGTCAA‑ATTCTTCCCATCGCTCCCCACGG‑TGATCGGTAGAATTTCCTTCGCCTAATacatcctgggcg                                                                                                             >  2:507344/5‑239 (MQ=255)                                                                                                                                    ggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAc                                                                                                           <  1:162730/251‑1 (MQ=255)                                                                                                                                     gAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcg                                                                                                          <  1:656035/251‑2 (MQ=255)                                                                                                                                          tAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTTTAGCTAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTc                                                                                                     >  1:113001/1‑251 (MQ=255)                                                                                                                                             agaggaccaatctccgccACCGATCCTCCGTGGCGGTTTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAAGCTGCGTCACCAGCTCGCAATCTCTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCG‑GCGAAACGGCGGTCAA‑GTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGAGTTTCCTACGCCTAATAACTCCTGAGCGACGGTTTCCAg                                                                                                  <  2:76227/238‑1 (MQ=255)                                                                                                                                                 gACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATc                                                                                                                                               >  1:199210/1‑202 (MQ=255)                                                                                                                                                 gACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATc                                                                                                                                               <  2:199210/202‑1 (MQ=255)                                                                                                                                                  aCCAAACGCCGCCACCGATCCCCCGTGGCGTTCTACCGGCAGGCCGTTTACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCGTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATGCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCgaga                                                                                             <  1:444914/251‑1 (MQ=255)                                                                                                                                                     aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGGCGGGTTCCAGCGGGAATg                                                                                          >  1:403346/5‑251 (MQ=255)                                                                                                                                                     aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATg                                                                                          <  2:306315/247‑1 (MQ=255)                                                                                                                                                                   atcccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGa                                                                                                                                                              <  1:348722/165‑1 (MQ=255)                                                                                                                                                                              cGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCATCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGAttt                                                                 <  2:260320/251‑1 (MQ=255)                                                                                                                                                                                   cACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGTTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                            <  1:157314/251‑1 (MQ=255)                                                                                                                                                                                    aCCGGCAGGCCGTTCACCGTCGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTAAGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAgcg                                                            <  2:330005/250‑1 (MQ=255)                                                                                                                                                                                       ggCAGGCCGTTCTCCGTCGCTCGTTCGATTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGTTTTATCTTCG‑GCGAAACGGCTGTCAA‑TTTCGTCCAGTCGTTCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGTGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                        <  1:688701/251‑1 (MQ=255)                                                                                                                                                                                       ggCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGCTTTATGGAAAATCTGCGTCACCAGCTCGCAATCATTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTGCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACGCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTc                                                        <  2:118258/251‑1 (MQ=255)                                                                                                                                                                                                   aCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGAt                                            <  1:13574/251‑1 (MQ=255)                                                                                                                                                                                                          cgcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTGTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCTATAATTa                                       <  2:650974/247‑1 (MQ=255)                                                                                                                                                                                                                cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTGCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCt                               <  1:65577/251‑1 (MQ=255)                                                                                                                                                                                                                                 aTTTCAGTTTTCTGGAAAATCTGCGTCACCAGCTCGCACTCTTTCAGGTTATAAGTCGCCAGAGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGCTCCC‑ACGGGTTATCTATAGATTTTCCTTCGCCTAATCACTCCTGCGCGACGGGTTCCAGCGAGAATGAAGAGAAAATCCCGAAAGCGTATTTCAGCGCCTCGATACCGTCGATAATTATACGCCATTTGGCCTTGGCaaaaa               >  2:78215/1‑250 (MQ=255)                                                                                                                                                                                                                                  tttCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCCA‑TTTTGGCCCTTCGATTCC‑ACGGGTTATCTATAGATTTTTCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGGGTATGAATAGAAATTCCAGAACCCGGATTTCAACACCTCGATACCTCCGCTAATTCGTCGGCCTTTAGACTGGGCaaacaa              >  1:269291/1‑247 (MQ=255)                                                                                                                                                                                                                                         tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGGAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGt      >  1:702365/5‑251 (MQ=255)                                                                                                                                                                                                                                         tttatgGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGTAAAAAAGACGCCg       <  1:555262/246‑1 (MQ=255)                                                                                                                                                                                                                                              ggAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGGTAATTTGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGgtttt  >  2:466846/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                         GGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTT  >  NC_000913/64514‑65000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑