| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 72,349 | C→G | A193A (GCG→GCC) | thiQ ← | thiamine ABC transporter ATP binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 72,349 | 0 | C | G | 94.6% | 339.9 / 11.6 | 111 | A193A (GCG→GCC) | thiQ | thiamine ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base G (71/34); total (75/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
TGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTG‑CCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGC > NC_000913/72106‑72594 | tGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTG‑CCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCtt > 1:659108/1‑251 (MQ=255) ggggTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCCTTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGATCGCGTGGCGCGCCGCGAGGCATCTTc > 2:139350/1‑251 (MQ=255) gggTTTAGGGGATTGTCTTGTGGGGTTTGCTTGATGCGCCG‑TGGACCGCGTCTTATCAGGCCTTAAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTcc < 2:543732/251‑1 (MQ=255) gggTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTcc > 1:374851/1‑251 (MQ=255) ttAGGTGATTGTCTTGTGCGGGTTGCCTGATGGGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGCCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCAca < 1:663953/250‑2 (MQ=255) aggggattgTCTTGTGCGGGTTGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGGCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGCCCACCACCGAGCGCGTGGCGGTCCGCGCGGCATCTTCCACACtg > 2:614622/9‑251 (MQ=255) ggggattgTCTTGTGCGGGTTGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACtgt > 1:207465/8‑251 (MQ=255) gattgTCTTGTGCGGGTTGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCCCACtgtg > 2:348239/5‑249 (MQ=255) gTCTTGTGCGGGTTGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGCGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGacac > 1:130820/1‑251 (MQ=255) tgtgCGGGTTGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACAcc < 1:496612/248‑1 (MQ=255) tGCCTGATGCGACGCTGA‑CCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCACTAACGTc > 1:402160/1‑251 (MQ=255) aTGCGACGCTTG‑CCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCAttttt > 1:23102/1‑251 (MQ=255) gCGACGCTCA‑CCGCGCCTTATCCGGCCTACAAAACGCACTGCCCGTAGGTCGGATAAGTTTTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACATCCCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATTCGCGCGGCATCTTCCACACTGTGTGACACCAGCAATAACGTCATTTTTtg < 1:207806/251‑1 (MQ=255) cgctgaccGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTtgctgc < 1:82102/245‑1 (MQ=255) cgcgTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGc < 1:391518/251‑1 (MQ=255) tATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑c < 1:649887/250‑1 (MQ=255) aTCAGGCCTACAAAACGCACTACCCGTAGGTCGGCTAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCACCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCCTTTTTTGCTGCTGGCAGCTC‑GGG‑CTc > 2:325396/1‑251 (MQ=255) aGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCAcc > 1:253871/1‑251 (MQ=255) aGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGGCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCAcc > 2:172565/1‑251 (MQ=255) cTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCg > 1:552869/1‑251 (MQ=255) aCAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTc < 1:419050/251‑1 (MQ=255) cAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCa < 1:538629/251‑1 (MQ=255) gcactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCAtct > 1:508501/8‑251 (MQ=255) cactgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCCTCTTCCACACTGTGTGACACCATCCATAACGTCCTTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCAtctc > 2:44122/7‑251 (MQ=255) tgtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCCTCAATAACGCAATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCACCTg > 2:149741/4‑251 (MQ=255) tACCCGTAGGTCAGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTg > 1:55711/1‑251 (MQ=255) gtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCt < 1:228813/247‑1 (MQ=255) gtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCt < 1:144894/247‑1 (MQ=255) gtccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTg > 1:664746/3‑250 (MQ=255) ccGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACg > 2:522392/1‑251 (MQ=255) cGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGt > 1:290148/1‑251 (MQ=255) gTAGGTCGGATAAGGTGTTCACGCCGAATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCTGCGACCACCACCGAGCGCTTGGCGATCCGCGCGGCATCTTCCTCACTGTGTGCCACATTCCATAACGTCCTTTTTTGCTACTGGCAGCTC‑‑TGTCTCCCCAGCGTCAGCATCTCCTGACGt > 2:630387/1‑250 (MQ=255) tAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTa > 1:215659/1‑250 (MQ=255) ggTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACg > 1:588556/1‑251 (MQ=255) ggATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCCGCATCTCCTGACGTAACGcagg > 2:485785/1‑248 (MQ=255) gATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCAca > 1:122417/1‑166 (MQ=255) gATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCAca < 2:122417/167‑2 (MQ=255) atcaggtgTTCACGCCGCCTCTGACCGTGCATACTAACCCGTAATACCCAATAGCGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACACTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGAGTGGCGATCCGCGCGGCCTCTTCCACACTGTGGAACACCATCAATAACTTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCATCAGCATCAGCATCTCCTGACGCAACGCCGGac > 2:219768/8‑250 (MQ=255) aTAAGGCTTTCACGCCGCACCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGAt < 2:659108/251‑1 (MQ=255) aggtgTTCACGCCGCATCTGACAGTGCATACTAACCAGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCCCCAGCGTCAGCATCTCCTGACGTACCGCCGGATCg > 2:389903/5‑250 (MQ=255) gccgcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAgaga > 1:77195/1‑250 (MQ=255) cgcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACg < 1:485785/251‑1 (MQ=255) gcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACgg > 2:236088/1‑251 (MQ=255) gcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACg < 1:236088/250‑1 (MQ=255) cAGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCACAGTCAGAATCCCCTGACGTAACGCCGGATCAAGCGAAGCGAACGTTTCATCg > 1:491846/1‑250 (MQ=255) agagCATACTAACCCGTACTCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGCTGCGCCCGTCGGCGACCACCACCGAGCGCGTGTCGATCCGCGCGACATCTTCCCCACAGTGTGACACCCTCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGAAGTAACTCCGGCTCGAGCGCCGAGAACGGTTCATCg > 2:122261/4‑249 (MQ=255) aGTGCATACTAACCCGTACTCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGAAGAAAACGGTTCATCgcg > 2:68298/1‑249 (MQ=255) aGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATCACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCgcg > 1:392351/1‑249 (MQ=255) gCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCa < 1:146419/250‑1 (MQ=255) aaCCCGTAATCCCAAATAGAGCTGAAGCACTCGCCTTACCGCTCACCAACTCCTTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGCCCACCACCGAGCGCGCGGCGATCCGCGCGGCATCTTCCACACTGTGTGCCACCATCAATACCGTCATTTTTTGCTGCTTGCAG‑TCAGTG‑CTCACCCGCGTCAGCATCTCCTGACGTCACGCCGCATCGCGCGCAgagacagcttcatctagcactcaaatc > 2:207164/1‑228 (MQ=255) cccGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTAATCGAGCAATacattcgg > 2:707109/1‑244 (MQ=255) ccccAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcg < 1:172565/250‑1 (MQ=255) cccAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCAAACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGAATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTcgcg > 1:348263/1‑251 (MQ=255) atagggctgAAGCACTAGCCTTACCGCTCAACAACTCATAGGTCTTACCATGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACAACATCAATACCGTCATTTTTTGCTGATGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCAGACGTAACGCCGGATCGAGCGCAGAGAACGGTACATCGAGCAATAAAATCGGCTGTTcgcgcccc > 2:22573/9‑247 (MQ=255) aTAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCAcc > 1:446767/1‑251 (MQ=255) gTGCTGAAGCACACGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCCGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGAATCTTCCACACTGTGTGACACAATCAAAAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CGCACCAGCATCAACATCTCCTGACGTAACGCCGGATCGATGGCAGCAACCGGTTCATCGAACAATAGAATCGGCtatgctcgcaca > 2:274140/1‑237 (MQ=255) aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGCTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGCCGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc > 2:229188/1‑251 (MQ=255) aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc > 1:33074/1‑251 (MQ=255) aaGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAAcgc > 1:92005/1‑251 (MQ=255) tCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAAc > 1:545725/1‑251 (MQ=255) ccTTACCGCTCAACAACTCAGTGGTATTACCCTGTCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGCTCCGCGCGGCATCTCCCACACTGTGGGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGcc < 2:130820/251‑1 (MQ=255) tACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCAcg > 1:203184/1‑250 (MQ=255) tACCGCGCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCa < 2:290148/249‑1 (MQ=255) cGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGAGGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGCGACAGGTTCATCGAGCAATAAAATCGGCTGTTAGCGCACCAG‑ACACCGCGCTAACGccccgcac > 1:75190/1‑245 (MQ=255) gCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑t > 1:377045/1‑251 (MQ=255) tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCCTTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGCGCACTAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTACCGCCCCgcgc‑tgt > 2:209334/1‑251 (MQ=255) tCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgt > 1:473237/1‑251 (MQ=255) ccaacacatcctggctcttACCCTGGCTGGCGATGCGCCCGTCGGCGACCACCACCGAGCGTGTGGCGAGCCGCGCGGCATCTTCCACACCGTTTGACACCATCTATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGATAACGGTTCATCGAGCAATAAAATCGGCTGGTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tg < 2:380863/232‑1 (MQ=255) acaacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgc > 1:449623/1‑251 (MQ=255) caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAATTCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgct > 1:256999/1‑251 (MQ=255) caacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACCGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgct > 2:146009/1‑251 (MQ=255) cacTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACCCCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACTTAACGCCGGATCGAGCGCAGAGAAAGGTTCATCGAGCAATAAAATCGGCTGTTCCCGAACAAG‑ACAACGCGGTAACGCCACgcca‑tgtcgctg > 2:401387/2‑251 (MQ=255) aacgcATTGGTTGTACCCGGCCAGGCGAGGCGCCCGTCGGCGACCGCCACCGAGCGCGTGGCGATCCGCGCGGCATCGTCCACACTGTGGGACACAATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCGGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg < 2:126087/247‑1 (MQ=255) gtcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGa < 2:377045/247‑1 (MQ=255) tcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGCAAAAC‑CGCTAACGCCCCCCCC‑TGTCGCTGCCCCCCGGa > 2:433216/4‑250 (MQ=255) ttccccTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg < 2:320021/236‑1 (MQ=255) ttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACgcgc‑tgtcgctg > 1:320021/2‑239 (MQ=255) tACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGaaa < 1:533882/249‑1 (MQ=255) tACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCAGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGaaa < 1:189778/249‑1 (MQ=255) aCCCTGCCAGGCGATGCGCCCGTCGGCGCCCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGCCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTAATCGAGCAATAAAATCGGCTGTTAGCGCACCGG‑ACAACGCTCTACCGCCACACGCCTG‑CGCTGACCGCCGGAAAggc > 2:317465/1‑249 (MQ=255) cAGGCGATGCGCCAGTCGGCGAACACCACCGAGAGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGACGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACAGCCGGAAAGCTCGccagg > 2:316903/1‑248 (MQ=255) gATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑CCAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAAcc > 2:505551/1‑251 (MQ=255) aTGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCACTAAAATCGGCTGTTCGCGCACCCG‑ACAACCCGCTAACGCCACGCCC‑TGTACCTGACCGCCGGaaatcccgccagggaaccc > 2:399025/1‑235 (MQ=255) gcgcCCGCCGGCGACCACCACCGAGCGCGGGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACcgcg < 1:490892/251‑1 (MQ=255) cGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCAtt < 1:348239/251‑1 (MQ=255) gTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACCCTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGccctt > 2:677608/1‑247 (MQ=255) ggCGACCACCACCGCGCGCGTGGCGATCCGCGCGGCATCTGCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGCTCGAGCGCAGAGACCGGTTCATCGAGCAATAACATCGGCTGTTCGCACACCAG‑ACCACGCGCTCACGCCACCCGC‑TGTAGCTGACCTCCGGAAAGCTCGCCAGAGAACCGCGcccttcat > 2:453347/1‑244 (MQ=255) ggCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACCCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAAt > 2:498405/1‑251 (MQ=255) gaccaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTAt > 1:311618/8‑251 (MQ=255) accaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGa < 1:687268/204‑1 (MQ=255) actacCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAAt < 1:325396/251‑1 (MQ=255) acCGAGCGCGTGTCGAGCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCCCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATccc < 2:449623/251‑1 (MQ=255) acCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGCTCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCCG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAAGTAACCGCGCCCTTAAATTATCAATccc > 2:367126/1‑251 (MQ=255) aGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATc > 1:433131/1‑251 (MQ=255) gcgcGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATACCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCt < 1:118723/251‑1 (MQ=255) gTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCTGAAAGCGCGACAGGCAACCGCGCCATTAAATTATCAATCCCCATCTggcg > 2:15894/1‑251 (MQ=255) gATCCGCGCGGCATCTTCCACACAGTGTGACACAATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCAAACGCCACGCGC‑TGTCGCTGACCGCCGGCAAACTACCCCGGTACACGCGCCATTAAATTAACAATCCCACTCTggcgggcg > 2:418436/1‑250 (MQ=255) cccccaccgcATCTGCCACGCGGGGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGCACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTATCGCCGCTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTggcgggcgtta < 2:23102/245‑4 (MQ=255) ccGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAG‑ACAACGCGCTAACGCCACGCGC‑TGGCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGAt > 2:336952/1‑249 (MQ=255) cgcgcgGCATCTTCCTCACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCCCCAG‑ACAACGCGCTAACGCCACGCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGc < 1:270353/251‑1 (MQ=255) | TGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTG‑CCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTC‑GTG‑CTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAG‑ACAACGCGCTAACGCCACTCGC‑TGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGC > NC_000913/72106‑72594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |