Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,875	T→C	G175G (GGT→GGC)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,875	0	T	C	90.5%	92.5 / 0.1	43	G175G (GGT→GGC)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base C (4/34);  total (5/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

AAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTG  >  NC_000913/71648‑72107                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         aaaaCTGAACATGCGTTGCATCAACACAGCATGTTCCCCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTgg                                                                                                                                                                                                                    <  1:20845/250‑1 (MQ=255)  aaaCTGAACTTGCGTTGCAGCAACCCAGCATGTGCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGTGACCGCTGGTTCTAATGGTGGCGGGAAGGCTGGATCTGCCGGTGGCTAAATTTATTTCGCCATATATTATCGGCTGCTTGCTGTGGCCGCCGGTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATGGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCa                                                                                                                                                                                                                  <  1:102577/251‑1 (MQ=255)    gttGAACAGGCGTTGCACCAACCCAGCATGTTCCCCCTTCTGGGCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGTCAATGGTGGCGGGAATGTTGGTTCTGCCGGTGGCAAAATGCATTACGCTGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGcgac                                                                                                                                                                                                                <  2:224300/249‑4 (MQ=255)      tGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCCAAATTCATTACGCCGGATATTATCGGCTGCCTGCTGTGGCCCCCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTCCCGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGcgacgg                                                                                                                                                                                                              >  1:38484/1‑246 (MQ=255)       gAACATGCGTTGCAGCAACACAGCATGTTCGCCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAGGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGcgacggc                                                                                                                                                                                                             <  2:353706/251‑7 (MQ=255)          cATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGGTTAAATGGGTGCTGCTGGCGAcggcggg                                                                                                                                                                                                          >  1:371943/1‑250 (MQ=255)           aTGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGGTATTCCAGCCGGGAAGCCGAGCGGCGAGTTTTAATGGTTGCTGCTGGcggcggcgggt                                                                                                                                                                                                         >  1:670979/1‑241 (MQ=255)                    aTCAACCCAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTgg                                                                                                                                                                                                <  1:320239/251‑1 (MQ=255)                                 gttccctagttgggtcggtcGTTTTTTTGGCCCGACTCGTCCGCTGGGGCCAATGGTGGCGGGAATGCGGGATCTGTCGGTGGCAAAATTCATTACGCCGAATATTAGCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCGGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGtggctggc                                                                                                                                                                                   <  2:276772/239‑1 (MQ=255)                                       cATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGGCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                             <  1:32503/251‑1 (MQ=255)                                                 ggtcgCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATg                                                                                                                                                                   <  1:360386/251‑1 (MQ=255)                                                               cccGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGGTGGCGACGGCGGGAATTGTGTGGGGTGGTGGGTGGCTGTGCTGGCGGtttttggggagggggtaa                                                                                                                                                      >  1:384496/1‑234 (MQ=255)                                                                 cGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTtggt                                                                                                                                                                                          <  1:143259/212‑1 (MQ=255)                                                                   gcgcgtccgtgggggtcAATGGGGGGGGGAATGTTGGAGCTGCCGGTGGAAAAATTAATTACGCCGAATATTAGCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTGAAATGGTTGCTGCTGGCGACGGCGGTATGTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaac                                                                                                                                                 <  2:404764/235‑4 (MQ=255)                                                                     gcgTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGGGCGGCGGTCGATATTCCTGCCGGGATGCAGAGCGGGGAGTTTAAATGGTTGCTGCTGGCAACAGCGGGGGTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCgggtttggggcgcgggggaaacggc                                                                                                                                                 >  1:287141/1‑226 (MQ=255)                                                                               gggacattggtggCGGGACTGCTGGATCTGCCGGTGGCAAAATTCATTTCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTGCTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATGTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGtttaag                                                                                                                                     <  2:686860/244‑4 (MQ=255)                                                                                      tggtggCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGgc                                                                                                                                                                 <  2:186724/216‑1 (MQ=255)                                                                                        ggggggggAATGTTGGATCTGCCGGTGGCAAAATTCATTCCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATGCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTAttt                                                                                                                            <  2:427604/246‑1 (MQ=255)                                                                                        ggggCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGTTTGCGACGGCGGTATTTGTGTGGGTTGGTGGCGGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTAttt                                                                                                                            <  1:307781/249‑1 (MQ=255)                                                                                          ggCGGGAATGCTGGATCTGGCGGGGGCAAAATTCATTACGCCGAATATTAACGGCTGCCTGCTGTGGCCACCGTTTTGCTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGt                                                                                                                          <  2:670979/251‑1 (MQ=255)                                                                                             gggAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgc                                                                                                                       <  1:168389/251‑3 (MQ=255)                                                                                              ggAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgcg                                                                                                                      <  1:621154/251‑4 (MQ=255)                                                                                                   gCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCggcc                                                                                                                 <  1:120214/251‑3 (MQ=255)                                                                                                      ggagctgccgttgtcaaaaTTCATTACGCCGAATATTAGCGGCTGCCTGCTGTGGCCACCGTTTTACTGCCTGCCTGGGATTCGGGCGGGAGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGtt                                                                                                              <  2:104046/235‑1 (MQ=255)                                                                                                             gcggttgcaatattcattaCGCCGAATATTATCGGTGGCCTGCTGTGGCCGCCTTTTTACTTCCTGCCGGGGATTCGGGGGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGGTTGTtgtg                                                                                                       <  2:361390/236‑1 (MQ=255)                                                                                                                       aatccattaCTCCGAATATTATCGGCTGCCTGTGGGGGCTACTGTGTTACTTCCTGCCTGGGATTCTGGGGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCg                                                                                             <  2:543892/246‑1 (MQ=255)                                                                                                                                cGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                             <  1:340579/162‑1 (MQ=255)                                                                                                                                     aaTATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAt                                                                               <  1:614108/251‑1 (MQ=255)                                                                                                                                             cggcggCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGTGGTGAGGTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTggctggct                                                                                                                                                                                  <  1:703100/144‑1 (MQ=255)                                                                                                                                               gCTGCCTGCTGGGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtggtg                                                                     <  2:248788/251‑1 (MQ=255)                                                                                                                                                  gcctgcTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgc                                                                   <  1:639679/250‑1 (MQ=255)                                                                                                                                                          gggccactgTTTTACGTCCTGCCGGGGATTCGGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGTAGAGCGGCGAGTTTAAATGGTGGCTGCTGGCGAGGGCGGTATTTGTGTGGGTTTGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCTGTAAAGCAACCGATCGTTTAAGTCATTATTTGGCGCGCGGCCGTTTGTTGTGGCTTACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                                                          <  2:326382/243‑1 (MQ=255)                                                                                                                                                          gggCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                                                          <  1:156471/250‑1 (MQ=255)                                                                                                                                                                    gtttcctccggccggggAGTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGGAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCg                                                 <  2:13277/236‑1 (MQ=255)                                                                                                                                                                           cctgcctgGGATGCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCg                                          <  1:564109/250‑1 (MQ=255)                                                                                                                                                                             ggcctgtgatgcTGGCGGGCGCGGCGACCGATAGTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTg                                       <  2:684186/240‑1 (MQ=255)                                                                                                                                                                                  gggATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGcc                                  <  1:49000/251‑1 (MQ=255)                                                                                                                                                                                            cgggcgCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGtt                                        <  2:180159/235‑1 (MQ=255)                                                                                                                                                                                                         cgatATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTa           <  2:321187/251‑1 (MQ=255)                                                                                                                                                                                                          gatATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTaa          <  1:153498/251‑1 (MQ=255)                                                                                                                                                                                                             atTCCAGCCGGAATGCAGTGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAg        <  2:560579/250‑1 (MQ=255)                                                                                                                                                                                                             atTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGt       <  1:313361/251‑1 (MQ=255)                                                                                                                                                                                                                  agCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTg  <  1:461048/250‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         AAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTG  >  NC_000913/71648‑72107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑