Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,824	A→T	P158P (CCA→CCT)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,824	0	A	T	93.0%	108.4 / NA	46	P158P (CCA→CCT)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base A (1/2);  major base T (14/26);  minor base G (0/3);  total (15/31)
Fisher's exact test for biased strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCG  >  NC_000913/71589‑72067                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    gTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTggcgggg                                                                                                                                                                                                                                      >  1:543892/1‑250 (MQ=255)   tttAAAAAGCCGTTGCATCGATGGTCATTTCTGAAGAAAAACAAAACATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTTCCGGTGGCAAAATTCATTACGCCGAATATTAACGGCTGCCTGCTGTGGACACCGTTTTTCTTCCTGCCTGGGCATCTggcgggggg                                                                                                                                                                                                                                    >  2:396293/1‑248 (MQ=255)    ttAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGGTTCTGGCGGGcgcg                                                                                                                                                                                                                                   >  1:326382/1‑251 (MQ=255)     tAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGcgcg                                                                                                                                                                                                                                   <  1:396293/250‑1 (MQ=255)     tAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGACGGGGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGACTGGGATTCTGGCGGGCgcgg                                                                                                                                                                                                                                  >  1:396221/1‑251 (MQ=255)                   aTCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTcc                                                                                                                                                                                                                     >  1:321187/1‑250 (MQ=255)                            aagttCTGAAGAAAACCAAAGCATTACTCAGTAAATCGGAACAGCCGTGTCATCAACATAGCATGTCCACCAGTCTGTGCGGTCGTTTGGTTGTGCCGAGGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCGGCCGGTGGCAAAATTCCTGACGCCGACTATTATCGCCTGCCTGCTGTGGCCACCGGTTTACTTCCTGCCTTGGATCCGGGCGGGCGCGTCGATCGATATTCCAGCCGGaag                                                                                                                                                                                                            <  2:396719/247‑2 (MQ=255)                             aTTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTCCAGCCGGAATGc                                                                                                                                                                                                          >  1:684186/1‑251 (MQ=255)                                 cTGAAGAAAAACAAAGCATTACTCGATAAACCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGACTGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATCTTATCGGCTGCCTGCTGTGGCAACCGTTTTAATTCCTGCCTGGGATTTGGGCGGGGGCGGCGAGCCATATTACCGCCGGGATGCag                                                                                                                                                                                                        >  2:153498/1‑249 (MQ=255)                                  tGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCCGCCGGAATGCAGAGc                                                                                                                                                                                                     >  1:13277/1‑251 (MQ=255)                                   gaagaaAAACAAAGCATTACTCGATAACACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCAAAACTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGATTTACTTCCTGCCTGGGATTCTGGCGGGGGCGGCGAGCGGTATTCCAGCCGGAATGCAGAGCg                                                                                                                                                                                                    >  1:276772/1‑251 (MQ=255)                                   aaagaaAAACAAAGGATTACGCTATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCCCCATTCGGGTCGGTCGCTTTGTTGGCCCGAGGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATAGTCCAGCCGGAATGCAGAGCg                                                                                                                                                                                                    <  2:686909/250‑1 (MQ=255)                                     agcaaaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGGATGCTGGATCTGCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGGGCGGCGAGCGATATTcaatccgggatgaagagcggg                                                                                                                                                                                                  >  2:120214/4‑231 (MQ=255)                                                            aaaaCTGAACATGCGTTGCATCAACACAGCATGTTCCCCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTgg                                                                                                                                                                            <  1:20845/250‑1 (MQ=255)                                                             aaaCTGAACTTGCGTTGCAGCAACCCAGCATGTGCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGTGACCGCTGGTTCTAATGGTGGCGGGAAGGCTGGATCTGCCGGTGGCTAAATTTATTTCGCCATATATTATCGGCTGCTTGCTGTGGCCGCCGGTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATGGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCa                                                                                                                                                                          <  1:102577/251‑1 (MQ=255)                                                               gttGAACAGGCGTTGCACCAACCCAGCATGTTCCCCCTTCTGGGCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGTCAATGGTGGCGGGAATGTTGGTTCTGCCGGTGGCAAAATGCATTACGCTGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGcgac                                                                                                                                                                        <  2:224300/249‑4 (MQ=255)                                                                 tGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCCAAATTCATTACGCCGGATATTATCGGCTGCCTGCTGTGGCCCCCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTCCCGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGcgacgg                                                                                                                                                                      >  1:38484/1‑246 (MQ=255)                                                                  gAACATGCGTTGCAGCAACACAGCATGTTCGCCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAGGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGcgacggc                                                                                                                                                                     <  2:353706/251‑7 (MQ=255)                                                                     cATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGGTTAAATGGGTGCTGCTGGCGAcggcggg                                                                                                                                                                  >  1:371943/1‑250 (MQ=255)                                                                      aTGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGGTATTCCAGCCGGGAAGCCGAGCGGCGAGTTTTAATGGTTGCTGCTGGcggcggcgggt                                                                                                                                                                 >  1:670979/1‑241 (MQ=255)                                                                               aTCAACCCAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTgg                                                                                                                                                        <  1:320239/251‑1 (MQ=255)                                                                                            gttccctagttgggtcggtcGTTTTTTTGGCCCGACTCGTCCGCTGGGGCCAATGGTGGCGGGAATGCGGGATCTGTCGGTGGCAAAATTCATTACGCCGAATATTAGCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCGGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGtggctggc                                                                                                                                           <  2:276772/239‑1 (MQ=255)                                                                                                  cATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGGCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                     <  1:32503/251‑1 (MQ=255)                                                                                                            ggtcgCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATg                                                                                                                           <  1:360386/251‑1 (MQ=255)                                                                                                                          cccGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGGTGGCGACGGCGGGAATTGTGTGGGGTGGTGGGTGGCTGTGCTGGCGGtttttggggagggggtaa                                                                                                              >  1:384496/1‑234 (MQ=255)                                                                                                                            cGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTtggt                                                                                                                                                  <  1:143259/212‑1 (MQ=255)                                                                                                                              gcgcgtccgtgggggtcAATGGGGGGGGGAATGTTGGAGCTGCCGGTGGAAAAATTAATTACGCCGAATATTAGCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTGAAATGGTTGCTGCTGGCGACGGCGGTATGTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGcaac                                                                                                         <  2:404764/235‑4 (MQ=255)                                                                                                                                gcgTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGGGCGGCGGTCGATATTCCTGCCGGGATGCAGAGCGGGGAGTTTAAATGGTTGCTGCTGGCAACAGCGGGGGTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCgggtttggggcgcgggggaaacggc                                                                                                         >  1:287141/1‑226 (MQ=255)                                                                                                                                          gggacattggtggCGGGACTGCTGGATCTGCCGGTGGCAAAATTCATTTCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTGCTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATGTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGtttaag                                                                                             <  2:686860/244‑4 (MQ=255)                                                                                                                                                 tggtggCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGgc                                                                                                                         <  2:186724/216‑1 (MQ=255)                                                                                                                                                   ggggggggAATGTTGGATCTGCCGGTGGCAAAATTCATTCCGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATGCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTAttt                                                                                    <  2:427604/246‑1 (MQ=255)                                                                                                                                                   ggggCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGTTTGCGACGGCGGTATTTGTGTGGGTTGGTGGCGGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTAttt                                                                                    <  1:307781/249‑1 (MQ=255)                                                                                                                                                     ggCGGGAATGCTGGATCTGGCGGGGGCAAAATTCATTACGCCGAATATTAACGGCTGCCTGCTGTGGCCACCGTTTTGCTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGt                                                                                  <  2:670979/251‑1 (MQ=255)                                                                                                                                                        gggAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgc                                                                               <  1:168389/251‑3 (MQ=255)                                                                                                                                                         ggAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTcgcg                                                                              <  1:621154/251‑4 (MQ=255)                                                                                                                                                              gCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCggcc                                                                         <  1:120214/251‑3 (MQ=255)                                                                                                                                                                 ggagctgccgttgtcaaaaTTCATTACGCCGAATATTAGCGGCTGCCTGCTGTGGCCACCGTTTTACTGCCTGCCTGGGATTCGGGCGGGAGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGtt                                                                      <  2:104046/235‑1 (MQ=255)                                                                                                                                                                        gcggttgcaatattcattaCGCCGAATATTATCGGTGGCCTGCTGTGGCCGCCTTTTTACTTCCTGCCGGGGATTCGGGGGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGGTTGTtgtg                                                               <  2:361390/236‑1 (MQ=255)                                                                                                                                                                                  aatccattaCTCCGAATATTATCGGCTGCCTGTGGGGGCTACTGTGTTACTTCCTGCCTGGGATTCTGGGGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCg                                                     <  2:543892/246‑1 (MQ=255)                                                                                                                                                                                           cGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                     <  1:340579/162‑1 (MQ=255)                                                                                                                                                                                                aaTATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCAt                                       <  1:614108/251‑1 (MQ=255)                                                                                                                                                                                                        cggcggCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGTGGTGAGGTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTggctggct                                                                                                                                          <  1:703100/144‑1 (MQ=255)                                                                                                                                                                                                          gCTGCCTGCTGGGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtggtg                             <  2:248788/251‑1 (MQ=255)                                                                                                                                                                                                             gcctgcTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgc                           <  1:639679/250‑1 (MQ=255)                                                                                                                                                                                                                     gggccactgTTTTACGTCCTGCCGGGGATTCGGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGTAGAGCGGCGAGTTTAAATGGTGGCTGCTGGCGAGGGCGGTATTTGTGTGGGTTTGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCTGTAAAGCAACCGATCGTTTAAGTCATTATTTGGCGCGCGGCCGTTTGTTGTGGCTTACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                  <  2:326382/243‑1 (MQ=255)                                                                                                                                                                                                                     gggCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                  <  1:156471/250‑1 (MQ=255)                                                                                                                                                                                                                                      cctgcctgGGATGCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCg  <  1:564109/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    GTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCG  >  NC_000913/71589‑72067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑