Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,701	T→C	R117R (CGT→CGC)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,701	0	T	C	93.0%	112.3 / ‑3.8	43	R117R (CGT→CGC)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (21/19);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATG  >  NC_000913/71478‑71946                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       gggggCTGGGAGCGCTTGTTGGCTGCGGCGAGTTAAGTTTCTGGGACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTGCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTg                                                                                                                                                                                                                             <  2:289168/250‑1 (MQ=255) cGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTg                                                                                                                                                                                                                             <  1:392368/251‑1 (MQ=255)                    tGGCAGCGGCGAGATAAGTTTCTGGCACGCATGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGGGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGTATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATACTTGGCGGGCGCTTTGGTGGCCCGACGCGACCGCTGGTGCCAATGGGGGCGGGGATgg                                                                                                                                                                                                          >  1:483648/1‑250 (MQ=255)                           ggcgAGTTAAGTTGCGGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGGTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCt                                                                                                                                                                                                   <  2:340538/251‑1 (MQ=255)                                           ggCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAAttc                                                                                                                                                                                  <  2:126273/251‑2 (MQ=255)                                            gCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTAGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAAttc                                                                                                                                                                                  >  1:267865/1‑249 (MQ=255)                                                       tggcagggattgtcggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGGTCT‑TCCGGTGGCAAAATTCATTACGCCGAat                                                                                                                                                                      >  1:177059/25‑251 (MQ=255)                                                                 ggtcggttgtctgctGGGCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACGCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCGGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGc                                                                                                                                                             <  1:319090/236‑1 (MQ=255)                                                                                gggCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGgccac                                                                                                                                             >  1:248788/1‑249 (MQ=255)                                                                                 ggcgactggttctccgtatggctggGCTGGCGTTTTAAAAAGCCGTTGCATCGGTGGTCATTGCGGAAGAAAAACAAAGCATTACTCGATAAATCTGAACATGCGTTGCATCAACACAGCATGGTCACCATTCTGGTCGGTCGCTTGGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGgcaacc                                                                                                                                            <  2:174077/233‑5 (MQ=255)                                                                                 ggCGACTGGATCTCCTTCTGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGgccacc                                                                                                                                            <  1:432338/251‑4 (MQ=255)                                                                                    gactgaaactccttctGGATGGGCTTGCGTTTTAAAAAGCCGCTGCATCGCAGGTCATTTCTGAAGAAAATCAAAGCATTACTAGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACTATTCTGGTCGGTCTCTTTGTTGGCACGACGCGTACGCTGGTGCCAATGGTGGCGGGGATGCTGGATCTTGCCGG‑GGCGAAATTCATTACCCAGAATATTTTCGGCGGCctgctgctgccaccctt                                                                                                                                         >  2:307781/13‑240 (MQ=255)                                                                                        ggatctccttctGGCTGGTGTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTCCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGAATCAACACAGCATGTTCACCATCCTGGTCGGTCGCTTTGTTGGCCCGATGCGGCCGCTGGTGCCAATGGTGGTGGGAATGCTGGATCT‑TCCGGTTGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTa                                                                                                                                      <  1:408613/242‑1 (MQ=255)                                                                                        ggatctccttctGGCTGGGCTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGtttt                                                                                                                                       <  1:384339/241‑1 (MQ=255)                                                                                                          gctGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTGCTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCg                                                                                                                                                               <  1:31735/205‑1 (MQ=255)                                                                                                            tGGCGTTTTAAAAAGCCGATGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGAGTCCGCTGGTGCCAATGGTGGCGGGGATGGTGGATCT‑GCCGGTGGCAAAATTCAATACGCCGTCTGTTTTCGGCTGTCTGCTGTGGGCACTGTTTTACTTGCTgactggggattgggg                                                                                                                 >  2:306171/1‑237 (MQ=255)                                                                                                                gTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTggcgggg                                                                                                             >  1:543892/1‑250 (MQ=255)                                                                                                                  tttAAAAAGCCGTTGCATCGATGGTCATTTCTGAAGAAAAACAAAACATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑TCCGGTGGCAAAATTCATTACGCCGAATATTAACGGCTGCCTGCTGTGGACACCGTTTTTCTTCCTGCCTGGGCATCTggcgggggg                                                                                                           >  2:396293/1‑248 (MQ=255)                                                                                                                   ttAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGGTTCTGGCGGGcgcg                                                                                                          >  1:326382/1‑251 (MQ=255)                                                                                                                    tAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGcgcg                                                                                                          <  1:396293/250‑1 (MQ=255)                                                                                                                    tAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GACGGGGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGACTGGGATTCTGGCGGGCgcgg                                                                                                         >  1:396221/1‑251 (MQ=255)                                                                                                                                  aTCGCTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTcc                                                                                            >  1:321187/1‑250 (MQ=255)                                                                                                                                      cTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGctgcct                                                                                                                                                        <  1:52392/186‑1 (MQ=255)                                                                                                                                      cTGGTCATTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCACGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATTCTGGATCT‑GCCGGTGGCAAAATTCATTACACCGAATATTATCGGctgcct                                                                                                                                                        >  2:52392/1‑186 (MQ=255)                                                                                                                                           aagttCTGAAGAAAACCAAAGCATTACTCAGTAAATCGGAACAGCCGTGTCATCAACATAGCATGTCCACCAGTCTGTGCGGTCGTTTGGTTGTGCCGAGGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCG‑GCCGGTGGCAAAATTCCTGACGCCGACTATTATCGCCTGCCTGCTGTGGCCACCGGTTTACTTCCTGCCTTGGATCCGGGCGGGCGCGTCGATCGATATTCCAGCCGGaag                                                                                   <  2:396719/247‑2 (MQ=255)                                                                                                                                            aTTTCTGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTCCAGCCGGAATGc                                                                                 >  1:684186/1‑251 (MQ=255)                                                                                                                                                cTGAAGAAAAACAAAGCATTACTCGATAAACCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGACTGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATCTTATCGGCTGCCTGCTGTGGCAACCGTTTTAATTCCTGCCTGGGATTTGGGCGGGGGCGGCGAGCCATATTACCGCCGGGATGCag                                                                               >  2:153498/1‑249 (MQ=255)                                                                                                                                                 tGAAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCCGCCGGAATGCAGAGc                                                                            >  1:13277/1‑251 (MQ=255)                                                                                                                                                  gaagaaAAACAAAGCATTACTCGATAACACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGGGGCAAAACTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGATTTACTTCCTGCCTGGGATTCTGGCGGGGGCGGCGAGCGGTATTCCAGCCGGAATGCAGAGCg                                                                           >  1:276772/1‑251 (MQ=255)                                                                                                                                                  aaagaaAAACAAAGGATTACGCTATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCCCCATTCGGGTCGGTCGCTTTGTTGGCCCGAGGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATAGTCCAGCCGGAATGCAGAGCg                                                                           <  2:686909/250‑1 (MQ=255)                                                                                                                                                    agcaaaaCAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGGATGCTGGATCT‑GCCGGTGGCAAAATTCATTAAGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGGGCGGCGAGCGATATTcaatccgggatgaagagcggg                                                                         >  2:120214/4‑231 (MQ=255)                                                                                                                                                                           aaaaCTGAACATGCGTTGCATCAACACAGCATGTTCCCCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTgg                                                   <  1:20845/250‑1 (MQ=255)                                                                                                                                                                            aaaCTGAACTTGCGTTGCAGCAACCCAGCATGTGCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGTGACCGCTGGTTCTAATGGTGGCGGGAAGGCTGGATCT‑GCCGGTGGCTAAATTTATTTCGCCATATATTATCGGCTGCTTGCTGTGGCCGCCGGTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATGGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCa                                                 <  1:102577/251‑1 (MQ=255)                                                                                                                                                                              gttGAACAGGCGTTGCACCAACCCAGCATGTTCCCCCTTCTGGGCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGTCAATGGTGGCGGGAATGTTGGTTCT‑GCCGGTGGCAAAATGCATTACGCTGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGcgac                                               <  2:224300/249‑4 (MQ=255)                                                                                                                                                                                tGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCCAAATTCATTACGCCGGATATTATCGGCTGCCTGCTGTGGCCCCCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGGTCGATATTCCCGCCGGAATGCAGAGCGGCGGGTTTAAATGGTTGCTGCTGGcgacgg                                             >  1:38484/1‑246 (MQ=255)                                                                                                                                                                                 gAACATGCGTTGCAGCAACACAGCATGTTCGCCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAAGGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGcgacggc                                            <  2:353706/251‑7 (MQ=255)                                                                                                                                                                                    cATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGGGGTTAAATGGGTGCTGCTGGCGAcggcggg                                         >  1:371943/1‑250 (MQ=255)                                                                                                                                                                                     aTGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGGTATTCCAGCCGGGAAGCCGAGCGGCGAGTTTTAATGGTTGCTGCTGGcggcggcgggt                                        >  1:670979/1‑241 (MQ=255)                                                                                                                                                                                              aTCAACCCAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTgg                               <  1:320239/251‑1 (MQ=255)                                                                                                                                                                                                     caGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGcaaa                                                                                                                                                                                      >  1:128294/1‑90 (MQ=255)                                                                                                                                                                                                           gttccctagttgggtcggtcGTTTTTTTGGCCCGACTCGTCCGCTGGGGCCAATGGTGGCGGGAATGCGGGATCT‑GTCGGTGGCAAAATTCATTACGCCGAATATTAGCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCGGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGtggctggc                  <  2:276772/239‑1 (MQ=255)                                                                                                                                                                                                                 cATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGGCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCt            <  1:32503/251‑1 (MQ=255)                                                                                                                                                                                                                           ggtcgCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATg  <  1:360386/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       CGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGTTTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATG  >  NC_000913/71478‑71946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑