Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,584	T→C	G78G (GGT→GGC)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,584	0	T	C	95.0%	105.9 / ‑5.6	41	G78G (GGT→GGC)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (12/26);  minor base G (1/1);  total (13/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCG‑ACTGGATTTCTTTCTGGCTGGGTTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTA  >  NC_000913/71340‑71814                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          aCAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGt                                                                                                                                                                                                                                     <  1:626357/251‑1 (MQ=255)                aaGCATTGCTGGAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTg                                                                                                                                                                                                                     <  1:701454/251‑1 (MQ=255)                      tGCTGGAACACTTTATTACCCAGTCTACCGTGTATTCATTGATGGCGGTGGTTTTGGTGGCCTTTCTGGAGTCGCTGTCGCTGGTCGGTTTGATTCTACCCGGTACTGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCGGCGGCGAGTTAATTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTATCTGCTGGTCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCg                                                                                                                                                                                                                <  1:108024/250‑1 (MQ=255)                           gAACACTTTATTACCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTc                                                                                                                                                                                                          <  1:603236/251‑1 (MQ=255)                                  ttattaCCCAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTTGCAGCGGCGAGGTAAGTTTTTGGCACGCCTGGCTGGCCGGGGTTGTCGGGTGTCTGCTGGGCG‑GCAGGGTCTCCTTCTTGCTGGGCTGGCGGTTT‑TAAAAGCCCTTGCATCGCTGGTCCTTTCtt                                                                                                                                                                                                   >  1:340538/1‑250 (MQ=255)                                      tcaccaatcttccGTGTATTCATTGATGGCGGGGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGGCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTg‑aag                                                                                                                                                                                               <  2:2508/239‑1 (MQ=255)                                         ccAATCTACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGaaaa                                                                                                                                                                                           <  1:498181/251‑1 (MQ=255)                                                 gtgtggtttCATTGAGGGCGGGGGGTTTGGAGTCCTTTCTTGAGGTGCTGGTGGTGGTGGGTTTGATTCTGCCCGGGATGGTGCTGATGTTGGGGCCGGGTGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTTGCACGCCTGGCTTGCGGGGATTGGCGGTTGGTTGCTGGGCG‑CCTCGATCTCTTTCTGGCTGTGAAGGCGTTTT‑AAAAAGCCGGTGCATCGCTGGTCATTACTG‑AAAAAAAACTAAGc                                                                                                                                                                                    <  2:24723/243‑1 (MQ=255)                                                     gtATTTATTGATGTCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCTGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGTAGGGATTGTCGGTTGTCTGCTGGGCG‑TCTGGATCTCCTTTTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCattac                                                                                                                                                                               <  1:82098/251‑1 (MQ=255)                                                       aTTCATTGATGGCGGTGGGGTTGGTGGCCTTTCGGGAGTCGTGGGCGCTGGTCGGTTTGATTCTGCCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCTGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCattactc                                                                                                                                                                             <  2:4773/251‑2 (MQ=255)                                                           aTTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCTCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATa                                                                                                                                                                         <  1:375760/251‑1 (MQ=255)                                                           aTTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGGTTGGCAGCGGCGAGTTAAGTTTCTGGCAAGCCTGGCTGGCAGGGGTTTTCGGGTGTCTGCTGGGGG‑ACTGGGTCTCCTTTTGGCGGGGCTGGCGTTTTTAAAAAACCGTT‑CATCCCTGGGCCTTTTTG‑AAGAAAAACaaaaaattaatcgat                                                                                                                                                                          >  1:289168/1‑238 (MQ=255)                                                             ggATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTTGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AATAAAAACAAAGCATTACTCGATaaa                                                                                                                                                                       <  1:465011/250‑1 (MQ=255)                                                                   cGGTGGTGTTGGTGGCCTTTCTGGAGGCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGaa                                                                                                                                                                 <  1:692147/251‑1 (MQ=255)                                                                              gtggCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCa                                                                                                                                                      <  1:132109/251‑1 (MQ=255)                                                                                        tGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGGTTGTCGGTTGTCTGCTGGGCG‑GCTGGGTCTCCTTCTGGCTGGGCTGGCGTTTT‑TAAAAGCCGGTGCATCGCTGGTCATTTCTG‑AAGAAAAAAAAAGCATTACTCGATAAAACTGAACATGCCGTGCATCAACACAGc                                                                                                                                            >  1:427604/1‑251 (MQ=255)                                                                                        tGGAGTCGCTGGCGCTGGCCGGTTTGATTCTCCCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGTTTGGCAGCGGCGGGTTAAGTTTCTGGCACGCCTGGCTGGCGGGGATTGTCGGTTGTCTGCTGGGGG‑ACTGGATTTCCTTCTGGCGGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCTTCAaccacgc                                                                                                                                            >  1:686860/1‑246 (MQ=255)                                                                                          gAGTCGCTGGCGCTGGTCGGTTTGATTCTACACGGTACGGTGCCGATGGCGGGGATGGGGACGCTTATTGGCAGCGGCGAGGTAAGGTTTTGGCACGCCTGGCTGGCAGGGATTGTCGGGTGTCTGGTGGGCGGAC‑GGATCTACTTCTGGCTGGGCTGGAGTTTT‑AAAAAACCGTTGGATCGCTTGGCATTTCTGCAGGAAAAACAA‑GAATTTCTCGATAAAACTGCACAAGCGATGCATCaaaccaacat                                                                                                                                          >  1:629131/1‑243 (MQ=255)                                                                                           ggTCGCTGGCGCTGGTCGGTTTGATGCTACCCGGTACGGTGCTGATGGCGGGGCTGGGGGCGCTGATGGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATg                                                                                                                                         <  1:434101/250‑1 (MQ=255)                                                                                              cgctggcgctggTCGGTTTGATTCTGCCCGGGACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAATCTGAACATGCGTTGCATCAACACAGCATGtt                                                                                                                                       <  1:530345/250‑1 (MQ=255)                                                                                                ctggcgctggTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGGAGGGATTGTCGGGTGGCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGGTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAAAAAAGCATTAATCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCAc                                                                                                                                    >  1:126273/1‑251 (MQ=255)                                                                                                         gTCGGTTTGATTCTACCCGGGACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGGTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACGCGATAAAACGGAACATGCGTTGCATCAACACAGCATGTTCACCAGTCTggg                                                                                                                           <  2:261483/251‑2 (MQ=255)                                                                                                              tttGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGGTTCTGGCACGCCTGGCTGGCAGGGGTTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTTTGGCTGGGCTGGCGTTTT‑TAAAAGCCGTTGCCTCGCTGGTCATTTCTG‑GAGAAAAACAAAGCATTACTCGGTAAAAATGAAAATACGTTGCATCAACACAGCAAGTTCCCCCTTCTggtcggtc                                                                                                                      >  1:104046/1‑251 (MQ=255)                                                                                                                tGATTCTTCCCGGTACGGTGCTGATGGGGGGGCTGGGGGCGCTGATTGGCAGCGGCGAGTTAAGTTTCGGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTGCACCATTCTGgtcggtcg                                                                                                                     <  2:384902/250‑1 (MQ=255)                                                                                                                                   gCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGt                                                                                                                                                                                                           <  1:322288/146‑1 (MQ=255)                                                                                                                                           gggggCTGGGAGCGCTTGTTGGCTGCGGCGAGTTAAGTTTCTGGGACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTGCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTg                                                                                         <  2:289168/250‑1 (MQ=255)                                                                                                                                           cGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTg                                                                                         <  1:392368/251‑1 (MQ=255)                                                                                                                                                              tGGCAGCGGCGAGATAAGTTTCTGGCACGCATGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGGG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGTATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATACTTGGCGGGCGCTTTGGTGGCCCGACGCGACCGCTGGTGCCAATGGGGGCGGGGATgg                                                                      >  1:483648/1‑250 (MQ=255)                                                                                                                                                                  aGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTc                                                                                                                               <  2:403397/190‑1 (MQ=255)                                                                                                                                                                  aGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTc                                                                                                                               >  1:403397/1‑190 (MQ=255)                                                                                                                                                                     ggcgAGTTAAGTTGCGGGCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGGTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCt                                                               <  2:340538/251‑1 (MQ=255)                                                                                                                                                                                     ggCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAAttc                                              <  2:126273/251‑2 (MQ=255)                                                                                                                                                                                      gCACGCCTGGCTGGCAGGGATTGTCGGTTGTCTGCTAGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAAttc                                              >  1:267865/1‑249 (MQ=255)                                                                                                                                                                                                 tggcagggattgtcggttgtctgctGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGGTCT‑TCCGGTGGCAAAATTCATTACGCCGAat                                  >  1:177059/25‑251 (MQ=255)                                                                                                                                                                                                           ggtcggttgtctgctGGGCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACGCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCGGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGc                         <  1:319090/236‑1 (MQ=255)                                                                                                                                                                                                                          gggCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGgccac         >  1:248788/1‑249 (MQ=255)                                                                                                                                                                                                                           ggCG‑ACTGGATCTCCTTCTGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGgccacc        <  1:432338/251‑4 (MQ=255)                                                                                                                                                                                                                            ggcgactggttctccgtatggctggGCTGGCGTTTT‑AAAAAGCCGTTGCATCGGTGGTCATTGCGG‑AAGAAAAACAAAGCATTACTCGATAAATCTGAACATGCGTTGCATCAACACAGCATGGTCACCATTCTGGTCGGTCGCTTGGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGgcaacc        <  2:174077/233‑5 (MQ=255)                                                                                                                                                                                                                               gactgaaactccttctGGATGGGCTTGCGTTTT‑AAAAAGCCGCTGCATCGCAGGTCATTTCTG‑AAGAAAATCAAAGCATTACTAGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACTATTCTGGTCGGTCTCTTTGTTGGCACGACGCGTACGCTGGTGCCAATGGTGGCGGGGATGCTGGATCTTGCCGG‑GGCGAAATTCATTACCCAGAATATTTTCGGCGGCctgctgctgccaccctt     >  2:307781/13‑240 (MQ=255)                                                                                                                                                                                                                                   ggatctccttctGGCTGGTGTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTCCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGAATCAACACAGCATGTTCACCATCCTGGTCGGTCGCTTTGTTGGCCCGATGCGGCCGCTGGTGCCAATGGTGGTGGGAATGCTGGATCT‑TCCGGTTGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTa  <  1:408613/242‑1 (MQ=255)                                                                                                                                                                                                                                   ggatctccttctGGCTGGGCTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCATTACTCGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGtttt   <  1:384339/241‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ACAGGCACATTATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTGGTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTGCTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGGCTGGCAGGGATTATTGGCTGCTTGATGGGCG‑ACTGGATTTCTTTCTGGCTGGGTTGGCGTTTT‑AAAAAGCCGTTGCATCGCTGGTCATTTCTG‑AAGAAAAACAAAGCACTACTTGATAAAACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCT‑GCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTA  >  NC_000913/71340‑71814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑