Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,680	G→C	G367G (GGC→GGG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,680	0	G	C	94.8%	180.1 / 3.0	61	G367G (GGC→GGG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base C (26/29);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

GTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATG  >  NC_000913/64434‑64912                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            gtgtgcgggATAAGGCTGCGCAAGGCTTTCCCCCAGACCGACGGGATCAACCAGAAAGGTGCGGATGAGAGACGGGTACAGGCTTTTATAGTCCAGCACCAGCCCT‑GAATCATAAAGCCC‑TGGCCGTGAATCAATCACGTAGCCGCCAGGGCGGGCGTGCGGCGGCACTGCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCGCCGAAccgcc                                                                                                                                                                                                                                           <  2:53577/242‑1 (MQ=255)         gATCAGGCTGCGCCATGCCTTCCTCCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTct                                                                                                                                                                                                                                 <  1:476642/251‑2 (MQ=255)             aGGCTGCGCCATGCCTGCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGAGGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCg                                                                                                                                                                                                                             <  2:252097/251‑1 (MQ=255)                cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTAcc                                                                                                                                                                                                                              <  1:97250/247‑1 (MQ=255)                cTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCAAccagtg                                                                                                                                                                                                                          >  2:97250/1‑247 (MQ=255)                 tGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGc                                                                                                                                                                                                                           >  1:171947/1‑249 (MQ=255)                              ccaccgGCCCGGCGGGATCAATCAGAAAGGGGCGGAGGAACGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAAGCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGACGTTCACCGtc                                                                                                                                                                                                            <  2:317089/251‑2 (MQ=255)                               caccaGCCTGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcg                                                                                                                                                                                                           <  2:240962/251‑3 (MQ=255)                                cccaGCCCGACGTGATGAATCAGAAAGGTGCGGATGACCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGTATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtcgc                                                                                                                                                                                                          <  1:321135/250‑4 (MQ=255)                                     cccaagggtatcaaacaAAAAGGGGCGGAGGATCGAGGGGTACAGGCTTTTAGAGCCCAGCAC‑AGGACTTAAATCAAAAGGCCC‑GGCCCGCGAATACATCAGTTAGCCCCCAGGGCGGGCGGGCGGGGCCACTTCGCGGAGATTAGGCGCGACAAACCCAGCGCGATGTATTCGCGAAAAATAGAGTGGACCAAATGCCGCCCCCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGtt                                                                                                                                                                                                     <  2:238911/242‑1 (MQ=255)                                           gggATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGt                                                                                                                                                                                                >  1:271167/1‑250 (MQ=255)                                              atcaatcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCAAGGAGCCGCCAGGGCTGGCGTGCGGCGGCCATTCGCCGAGATTCGGCGCGTCATAAACAGCGCGATGCATTCGCGGACAATAGAGATGATCAAGAGCCGCCAACGAACCACCGTGTCGGTACACCGGCAGGCCGTTCACCGTTGcccctccaaggcaa                                                                                                                                                                                             >  2:157314/1‑239 (MQ=255)                                                   tcaGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCAcccgccctcgttcggtgtacaatggc                                                                                                                                                                                       >  2:630101/1‑227 (MQ=255)                                                       aaaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGACCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGa                                                                                                                                                                                   <  1:229414/251‑1 (MQ=255)                                                        aaGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGAt                                                                                                                                                                                  >  1:124074/1‑251 (MQ=255)                                                             gCGGATTATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCTCTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCa                                                                                                                                                                              <  1:298861/250‑1 (MQ=255)                                                                gatgagagACGGGTACAGGCTTTGATAGTCCAGCACCAGTACA‑GAATCATAAAGCCCGTGGC‑GTGAATCCATCACGGAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTATGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCAACGATCCCCCGTGGAGGTCTACCGGCAGGCCGTTCACCGTCGCGCGTTCGAGTAAAAATGGCATGATTTCAGttt                                                                                                                                                                          <  2:104982/244‑1 (MQ=255)                                                                                 ggCTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTc                                                                                                                                                         >  1:470418/1‑251 (MQ=255)                                                                                   cTTTTATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGCTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCCGTTTTATgggaaaaccgcgtcac                                                                                                                                                       >  2:190035/1‑237 (MQ=255)                                                                                    ttttATAGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCAcc                                                                                                                                                      >  1:363444/1‑251 (MQ=255)                                                                                         taGTCCAGCACCAGTACC‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATCCGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGATCACCGGCGCTCGTTCGGGTAAAAAAGGCCTTATTTCCGTTTTATTGAAAATCTGCGTCACCaacac                                                                                                                                                 >  2:555262/1‑247 (MQ=255)                                                                                           agtccggctccaggtccgAATAATAAAGCCC‑GGGCCGTGAATCCATCACGTAGCCGCCAGGGCTTGCGGGTGGGGGCACTACGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAACCTGCGTCACCAGCTCg                                                                                                                                                <  2:278578/234‑1 (MQ=255)                                                                                                          ccgAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTta                                                                                                                                 <  2:144743/249‑1 (MQ=255)                                                                                                            gAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCCGGCCGTTCACCGTCCCCCGGTCGGGTAAAAATGGCCTGGTTTCAGTTTTTTGGAAAATCTTCGTCACCAACTCGCAATCTTTTCGGTtat                                                                                                                                >  2:162730/1‑250 (MQ=255)                                                                                                            aaaTCATAAAGCCC‑TGGCCGGTAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACGTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCTTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGATAATCTGCGTCACCAGCTCGCAATCTTTCAGGTtata                                                                                                                               <  2:330047/250‑1 (MQ=255)                                                                                                                  tAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCATCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGtcgc                                                                                                                         >  1:563692/1‑248 (MQ=255)                                                                                                                               cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCCGGTTATAAGTCGCCAGCGCAGGttt                                                                                                             >  1:114403/1‑251 (MQ=255)                                                                                                                                  gAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATc                                                                                                          <  1:502053/251‑1 (MQ=255)                                                                                                                                     cccATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTc                                                                                                       <  2:271167/250‑1 (MQ=255)                                                                                                                                      ccaacACGTAGGCGCCAGGGCGGGCGTGTGGCGGCTCTTCGCCGTGATTAGTCGCGACATACACAGCGCGGGGCCTACGAGGAAAATAGAGATGACCAAACGCCTCCACCGATCCCCCGTGGCGGTCGAGCGGCAGGCCTTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGTTTATCTTCg                                                                                                      <  2:547186/247‑1 (MQ=255)                                                                                                                                        aTCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑Gc                                                                                                   >  1:425597/1‑251 (MQ=255)                                                                                                                                          cACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGa                                                                                                 <  1:4777/251‑1 (MQ=255)                                                                                                                                          cACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCg                                                                                                  >  1:211800/1‑250 (MQ=255)                                                                                                                                                     cAGGGCTGGCGTGTGGCGGCACTTCGCCGAGAGTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCa                                                                                      <  2:425597/251‑1 (MQ=255)                                                                                                                                                      aGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCaa                                                                                     <  1:617974/251‑1 (MQ=255)                                                                                                                                                        ggcgggcgagtggcggcACTTCGCCGAGATTAGGCTCGACATAACCAGAGCGGGGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCTATACTCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCGCGCAATCTTTTAGGTTATAAGTCGCTAGCGCAGGTTTATCTTCG‑GCGAAACGTCGGTCAA‑tt                                                                                  <  2:253924/240‑1 (MQ=255)                                                                                                                                                        ggCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑tt                                                                                  >  1:137699/1‑251 (MQ=255)                                                                                                                                                             gCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGGTTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTAGCCAGCGCAGGTTTATCTTCGAGC‑AAACGGCGGGCAA‑ttttttc                                                                             >  2:170983/1‑247 (MQ=255)                                                                                                                                                                     cggcACTTCGCCGAGATTAGGCGCGACTTAACCAGCGCGGTGCATACGAGGAAAATAGAGATGGCCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGAt                                                                     <  2:470418/251‑1 (MQ=255)                                                                                                                                                                               ccGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCCTTCGATCCC‑ACggggt                                                          >  1:212743/1‑249 (MQ=255)                                                                                                                                                                                cGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGt                                                                                                                                                          <  1:567056/157‑1 (MQ=255)                                                                                                                                                                                    aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCgt                                                     >  1:364266/1‑250 (MQ=255)                                                                                                                                                                                    aTTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTGCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGa                                                     <  1:339844/251‑1 (MQ=255)                                                                                                                                                                                     ttAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTAACGAt                                                    >  1:650974/1‑251 (MQ=255)                                                                                                                                                                                           gcgACAGAACCAGCGCTGTGCATACGCGGAAAATAGAGATGACCAAACGCCGCCTCCGATCCCCCGTGGCGGGCTACCGGCAGGCCGTTCACCGTCGATCGTTCGAGTAAAAATGGCTGGAGTTCAGTTTTATGGAAAAGCTGCGTCCCCAGCGCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑GTGCGTCCATTCGATCCC‑GCGGGTTATCGATAGAttt                                              <  2:619079/251‑1 (MQ=255)                                                                                                                                                                                            cgACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGCGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTTCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGGCAAATTTCG‑CCCTTCGTTCCC‑CCGGGGTATCGAGAGAtttt                                             >  2:13574/1‑251 (MQ=255)                                                                                                                                                                                             gACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGAttttt                                            >  1:118258/1‑250 (MQ=255)                                                                                                                                                                                              aCATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTcc                                           <  1:512754/251‑1 (MQ=255)                                                                                                                                                                                                                atacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑CCGGGTTATCGATAGATTTTCCTTCGCCtcattacttctt                         >  1:306315/7‑240 (MQ=255)                                                                                                                                                                                                                atacgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTg                         <  1:701523/245‑1 (MQ=255)                                                                                                                                                                                                                  acgaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGGTAGATTTTCCTTCCCCTAATAACTCCTggg                       >  1:80321/5‑249 (MQ=255)                                                                                                                                                                                                                     ggaaaaaTAGAAATGCCCAAGTGCCGCCCCGGAACCGCCGGGTCAGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGa                    <  2:211800/248‑1 (MQ=255)                                                                                                                                                                                                                     aggcaaaTAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGCTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAAAGGCGGTCAA‑ATTCTTCCCATCGCTCCCCACGG‑TGATCGGTAGAATTTCCTTCGCCTAATacatcctgggcg                     >  2:507344/5‑239 (MQ=255)                                                                                                                                                                                                                      ggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAc                   <  1:162730/251‑1 (MQ=255)                                                                                                                                                                                                                       gAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAcg                  <  1:656035/251‑2 (MQ=255)                                                                                                                                                                                                                            tAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTTTAGCTAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTc             >  1:113001/1‑251 (MQ=255)                                                                                                                                                                                                                               agaggaccaatctccgccACCGATCCTCCGTGGCGGTTTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAAGCTGCGTCACCAGCTCGCAATCTCTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCG‑GCGAAACGGCGGTCAA‑GTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGAGTTTCCTACGCCTAATAACTCCTGAGCGACGGTTTCCAg          <  2:76227/238‑1 (MQ=255)                                                                                                                                                                                                                                   gACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATc                                                       >  1:199210/1‑202 (MQ=255)                                                                                                                                                                                                                                   gACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATc                                                       <  2:199210/202‑1 (MQ=255)                                                                                                                                                                                                                                    aCCAAACGCCGCCACCGATCCCCCGTGGCGTTCTACCGGCAGGCCGTTTACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCGTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATGCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCgaga     <  1:444914/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGGCGGGTTCCAGCGGGAATg  >  1:403346/5‑251 (MQ=255)                                                                                                                                                                                                                                       aaacgccgccACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATg  <  2:306315/247‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            GTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACT‑GAATCATAAAGCCC‑TGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCG‑GCGAAACGGCGGTCAA‑TTTCGTCCATTCGATCCC‑ACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATG  >  NC_000913/64434‑64912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑