Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,172	C→T	A2V (GCT→GTT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,172	0	C	T	100.0%	205.0 / NA	62	A2V (GCT→GTT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/32);  total (30/32)

GGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGG  >  NC_000913/13929‑14415                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ggAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCTCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCa                                                                                                                                                                                                                                               >  2:388731/1‑250 (MQ=255)      tGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCACTTTAAAAAAGATGGTTAAGCAAGatt                                                                                                                                                                                                                                          >  1:340490/1‑250 (MQ=255)       ggCAAAGGTTTCCCAGAACCTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAAGTTGAAGAAGTCAAAGACAAAAAATAATCGCCCGATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGatt                                                                                                                                                                                                                                          <  1:493981/249‑1 (MQ=255)              ggttCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCGTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCCATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATGTCCGCGCCGCCCGTGCAGTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTAGTACgaga                                                                                                                                                                                                                                 <  2:361735/249‑1 (MQ=255)              gTTTCCCAGAAACTGATGGAAATCGACCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAACTTGAAGACGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGACGGGGAATTTACTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACg                                                                                                                                                                                                                                    >  1:350215/1‑248 (MQ=255)                 tCCCAGAAACTGATGGAAATCGCCCAGCATCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGAtt                                                                                                                                                                                                                               <  2:569823/250‑1 (MQ=255)                     aGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGCTGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAgg                                                                                                                                                                                                                          >  2:355280/1‑251 (MQ=255)                         aCTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGACTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACAGGCGAAGGGGAATTTCATCTCCGCCCGAGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGtt                                                                                                                                                                                                                      >  2:350196/1‑251 (MQ=255)                         aCTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGtt                                                                                                                                                                                                                      <  1:109269/251‑1 (MQ=255)                            gATGGAAAGCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGttt                                                                                                                                                                                                                     <  2:603700/249‑1 (MQ=255)                               ggAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCaaa                                                                                                                                                                                                                <  1:131763/251‑1 (MQ=255)                                  aaacgtccAGCAGAAACATGCCCAGGAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGGCGACGCGGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCGCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTAGTACGAGATTTTAGGCGTTTTCAAAACa                                                                                                                                                                                                             <  2:583475/245‑1 (MQ=255)                                                     gCCCAGCAGCAGACTGCAGGTGCTGATGCTTCTGCAAACCACGCGAAAGATGAAGATGTTGTCGACGCTGAATATGAAGAAGTCAAAGACAAAAAAGAATCGTCCTATAAACGGGTAATTATACTGACACGGGCGAAGTGGAATTTCCTCGCCGCCCGTGCTCTCATCTAGGGTCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTATGCGTTTCCAAAACAGCGGAAGAGCGTGAAATc                                                                                                                                                                                           <  2:279502/250‑1 (MQ=255)                                                       ccAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCa                                                                                                                                                                                                                                               <  1:152730/196‑1 (MQ=255)                                                       ccAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCa                                                                                                                                                                                                                                               >  2:152730/1‑196 (MQ=255)                                                         agcagcagACTGGCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAATCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATTGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATAAGaaa                                                                                                                                                                                      <  2:74231/251‑1 (MQ=255)                                                               cgACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAACAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGTGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGTGATTTTCGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCt                                                                                                                                                                                 >  2:279350/2‑250 (MQ=255)                                                               agACTGCCTGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTa                                                                                                                                                                                <  2:599229/251‑1 (MQ=255)                                                                    gCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATTACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACaaa                                                                                                                                                                            <  1:706423/250‑1 (MQ=255)                                                                        gTGCTGATGCTTCTGCAAACAACGCGAACGATGACGATGTTGTCGACGCTGAGTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTCTAACCGGGTAATTATACTGACACGGGAGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCACTTTAAAAACGATGGTTAAGCAAGATTATTACGCGATTTTAGGCGTTTCCAAAACAGCGGCAGAGCGTGAAATCAGAACGGCCTACAACCGCCt                                                                                                                                                                       >  2:343841/1‑251 (MQ=255)                                                                        gTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCt                                                                                                                                                                       <  1:574820/251‑1 (MQ=255)                                                                                       cAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATacc                                                                                                                                                         >  2:467340/1‑250 (MQ=255)                                                                                           aaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCg                                                                                                                                                    <  2:92770/250‑1 (MQ=255)                                                                                            aacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCgg                                                                                                                                                   <  1:355280/251‑1 (MQ=255)                                                                                                cgAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCg                                                                                                                                               <  1:9886/251‑1 (MQ=255)                                                                                                      aTGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCa                                                                                                                                         <  1:388731/251‑1 (MQ=255)                                                                                                          cGATGTTGTCGACGCTGACTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATAATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCAGGAAATACCACCCGGACCGTAACCAgggg                                                                                                                                     >  2:478434/1‑250 (MQ=255)                                                                                                           gATGTTGTGGCCGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATAGCCCTATAAACGGGTAATTATACTGACACGGGCGAAAGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAGGCCAGATTATTACGAGATTTTAGGAGTTTCCAAAAAAGCGGAAGGGCGTGAAATCAGAAAGACATACAAACGCCTGGCCATGAAATACCAACCGGAACGTAACCAggggg                                                                                                                                    >  2:43499/1‑249 (MQ=255)                                                                                                           gATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAACGCCTACAAACGCCTGGCCATTACTTACCACCCTTTCCGTAACCAGGGTg                                                                                                                                    <  1:504253/251‑1 (MQ=255)                                                                                                              ggtgtCGTCGCTGAATTTGAAGGAGTCACAGACAAAAACTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAATACAGCGGCAGAGCGTGATATCAGAAAGGCCTACAAACGCCTGGCCATGCAATACCAACCGGACCGTAAACAGGGAGAc                                                                                                                                  >  2:11858/3‑250 (MQ=255)                                                                                                                 gtCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAag                                                                                                                              <  1:504847/251‑1 (MQ=255)                                                                                                                     aCGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCGCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTATGGGCTATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTGTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGc                                                                                                                          <  1:605612/251‑1 (MQ=255)                                                                                                                             tttGAAGAAGTCAGAGACAAAAAATAATCGCCCTATAAACGGGGAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGCTTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGTCCATGAAATACCACCCGGACCGTAACCAGGGAGACAAAATGGCCGAGGCg                                                                                                                   >  2:79174/1‑250 (MQ=255)                                                                                                                                 aagaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAtt                                                                                                              <  1:341087/251‑1 (MQ=255)                                                                                                                                  agaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAttt                                                                                                             <  1:66222/251‑1 (MQ=255)                                                                                                                                  agaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAttt                                                                                                             <  2:319645/251‑1 (MQ=255)                                                                                                                                     agTCAAAGACAAAAAATAAACGCCCTAGAAACGGGAAATTATACTGACAAGGGAGAAGGGGAATTTCCTATCCGCCCGTACATTCATATAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGAGTGAGATCAGACAGGCCTACAAATGAATGGCTATGAAATAGTAACCGGACAGTAACCCGGGGAAAAAAGAGGCCGAGGGGAAATTTaa                                                                                                           >  2:369270/1‑250 (MQ=255)                                                                                                                                                aaaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGa                                                                                               <  1:1400/251‑1 (MQ=255)                                                                                                                                                 gaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGcc                                                                                                                         <  2:473745/223‑1 (MQ=255)                                                                                                                                                 aaaaTAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGcc                                                                                                                         >  1:473745/1‑224 (MQ=255)                                                                                                                                                 aaaaTAATCGCCCTATAAACGGGTAATTATACTAACACGTGCGAAGGGGACTTTACTCACCGCCCGTGAATTCATCTAGTGGAAATTTAAAAAAGATGGTTAAGCACGATTATTACGAGATTTTAGGCGTTTCCCAAACAGAGGAAGAGCGTGAAATCAGAAAGGCCTAAAAACGCCTGGCCATGAAATACCACCCGAACCGTAACCCGGCTGAAAAAGAGGCCGAGGAGCAATTTAAGgagaaccagga                                                                                               >  2:599585/1‑243 (MQ=255)                                                                                                                                                   aataatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGc                                                                                            >  1:558713/1‑251 (MQ=255)                                                                                                                                                    ataatCGCACTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAag                                                                                                                                                                                                      >  2:293149/1‑144 (MQ=255)                                                                                                                                                    ataatCGCACTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAag                                                                                                                                                                                                      <  1:293149/144‑1 (MQ=255)                                                                                                                                                            ccTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGt                                                                                   <  1:458849/251‑1 (MQ=255)                                                                                                                                                                  aaCGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGAc                                                                             >  1:193923/1‑251 (MQ=255)                                                                                                                                                                      ggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGAc                                                                         >  1:253898/1‑251 (MQ=255)                                                                                                                                                                            tataCTGACACGGGCGAAGTGGAATTCACTCTCCGCCCGTGCACGCATCTATGTGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACATCGGAAGAGAGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGATGCCGAGGCGAAATTTAAAGAGATCAAGGCATCTTATGAAGTTCTGACCGCCTCGCa                                                                    <  2:257876/250‑1 (MQ=255)                                                                                                                                                                                    cacGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGTCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                           <  2:680054/251‑1 (MQ=255)                                                                                                                                                                                                ggAATTTCCTCTCCGCCCGTGAATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTT‑AGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTCCAAACGCCTGGCCATGAAATACCACCCGGTCCGTAACCAGGGTGACAAAGAGGCCGAGTCGAAATTTAAAGAGATCACGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGAGGCATAccatta                                               >  2:605067/1‑245 (MQ=255)                                                                                                                                                                                                         tctcCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAAAGAGCGGCATACGACCCGTATGGTc                                       >  2:382310/1‑250 (MQ=255)                                                                                                                                                                                                                  gTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGATATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGtt                             <  2:393638/251‑1 (MQ=255)                                                                                                                                                                                                                  gTGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGtt                             >  1:393638/1‑251 (MQ=255)                                                                                                                                                                                                                   tGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGAcc                                                                                                                                                <  2:45239/135‑1 (MQ=255)                                                                                                                                                                                                                   tGCATTCATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGAcc                                                                                                                                                >  1:45239/1‑135 (MQ=255)                                                                                                                                                                                                                         cATCTAGGGGCAATTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGACATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                       >  1:319767/1‑250 (MQ=255)                                                                                                                                                                                                                                    aaTTTAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGCAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGAGGCATggg           >  1:358677/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttAAAAAAGATGGTTAATCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcg         <  1:343841/250‑1 (MQ=255)                                                                                                                                                                                                                                        tAAAAAAGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGCCTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGTTGCGTTTGAGCAAGGTGGCATGggcggg       >  1:196116/1‑250 (MQ=255)                                                                                                                                                                                                                                         aaaaaaGATGGTTCAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcg      >  1:139752/1‑251 (MQ=255)                                                                                                                                                                                                                                          aaaaaGATGGTTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcgg     >  1:349905/1‑251 (MQ=255)                                                                                                                                                                                                                                             aaGATGGTTAAGCACGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGCAGAGCGTGAAGTCAGAAAGGCCTACAAACCCCTGGCCATGAAATACCACCCGAACCGTAACCAGGGTGACAAAGAGGCCACGGCGAAATTTCAAGAGAACAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATATGTATGGTAACGCGACGTTTGAGCACGGAGGCATGGGcggcggcgg  >  2:56985/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGG  >  NC_000913/13929‑14415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑