Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,287,7100GA25.0% 46.2 / 11.2 32intergenic (+43/‑54)ytcB/nrfAprotein YtcB/cytochrome c552 nitrite reductase
Reads supporting (aligned to +/- strand):  ref base G (24/0);  new base A (0/8);  total (24/8)
Fisher's exact test for biased strand distribution p-value = 9.51e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTC  >  NC_000913/4287462‑4287951
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
gATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCACTGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGa                                                                                                                                                                                                                                                  >  2:97660/1‑250 (MQ=255)
              aGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAAcc                                                                                                                                                                                                                                   >  1:637945/1‑251 (MQ=255)
              aGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAAcc                                                                                                                                                                                                                                   >  2:210686/1‑251 (MQ=255)
               gAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCg                                                                                                                                                                                                                                  >  2:506638/1‑251 (MQ=255)
                  ttGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGcc                                                                                                                                                                                                                                >  1:619347/1‑250 (MQ=255)
                     tgCAAATAAACTGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTg                                                                                                                                                                                                                            >  2:216784/1‑251 (MQ=255)
                         aaTAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAAAAATAAAAACCGCCATTGcaac                                                                                                                                                                                                                        >  2:220510/1‑251 (MQ=255)
                                ggCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCAATTGCAACAATGgcg                                                                                                                                                                                                                 >  2:527494/1‑251 (MQ=255)
                                           ttttGAAGGTCAGAAACAAAAGTTAATTAATTCTTTGAGGAACTTGCATTTATGCATGCTTTTGAAAAAGAGAAAGATACTGACTAACTAAAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGCCAACATCCACAG‑TGTGGTTTTTTGTTCCACATGGGGGCGAGAAATGTAAGAACAGTGTGGGTTCGTTT‑ACTGTCGTAAAAAAAAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGAt                                                                                                                                                                                                       <  1:465090/250‑1 (MQ=255)
                                               gAAGGTCAGCAACAAAAGTTGATTACTTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTCTTTGTTACACATAGGGGCAAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCAATTGCAACAATGGCGCAATTCGGATGAAGc                                                                                                                                                                                                  >  2:546723/1‑251 (MQ=255)
                                                        aaacaaAAGTTGAATATTTCTTTGAGAAACATGAAGTTATGCATGCTGTTAAAAAAGAGGAAAATACTGACTAACTCAAAAGTGTTATTTTACATGCATTTACAATTGATAAAAGACAACATTCACAG‑TGTGGTTATTTTTTACACATAGGGGCGAGAAATGTCATGACAGTGTAGGTGCGTTT‑ACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGAc                                                                                                                                                                                         <  1:666994/250‑1 (MQ=255)
                                                             aaaGTAGATAAATTTTTGGAGGAACATGCATTTATGTATGATGTTAAAAAAGAGGAATAACCTGACTAATTCAAATGGGGTTTTTTAAATCCATTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTGGTTACACATGGGGGCGAGCATTGTCATGACAGTGTAGGGGCGTTT‑ACTGTCGTAAAAAAAAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGa                                                                                                                                                                                    <  1:622460/251‑1 (MQ=255)
                                                                       caTTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGCCGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTACAGACAACCTTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑CCTGTCGTGACAAACAATAAAAACCGCCATTGCCACAATGGCGCATTTCGGATGAAGCCCCTTTGACAAGGATAAAAATac                                                                                                                                                                           >  2:290460/2‑249 (MQ=255)
                                                                             ttGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAAATCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATACAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGAAAAGGATAAAAATAAacgcacg                                                                                                                                                                    >  2:284090/1‑251 (MQ=255)
                                                                             ttGAGAAACATGCAGTTAGGAATGTTGTTAAAAAAGAGGAAAATACTGACTAACTAAAAAGTGGTATTTTTCATGCATTAACAATTAATAAAAGAAAACATTCAAGG‑TGTGTTTATTTGTAACACATAGGGGCGAGAAATGTCATGACGGTGTAGGTGGGTTT‑ACTGTCGTAAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgc                                                                                                                                                                       <  1:271424/248‑1 (MQ=255)
                                                                              tGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgcacgc                                                                                                                                                                   >  1:204216/1‑251 (MQ=255)
                                                                                      atgcgttgatgtatgtggttaaaaaaGAGGAAGATACGGACAAACTAAAAAGTGTTATTTTACATGATCTTAAAATTGATTAAAGAAAACATCCACGG‑GGTGTTTTTTGGTAACAAAGGGGGGGGAAAATTGTAATGACAGTGTAGGTGCGGTT‑ACTGTGGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCtt                                                                                                                                                           <  1:213869/230‑1 (MQ=255)
                                                                                        gCAGTTATTGATTCTGTTAAAAAAGAGAAAGATACTGACTAACTCAAAAGGGGTTTTTTACATGCATTTAAATTTAATAAAAGACAACATTCACAG‑TGTGTTTTTTTTGTACACATGGGGGCGAGAAAGTTCATAACAGT‑TGGGGGGGGTTTATGGTGGAAAAAAAAAAAAAAATCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCa                                                                                                                                                         <  1:477702/251‑1 (MQ=255)
                                                                                                                gagGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGCTTCCTtttttctttt                                                                                                                                 >  1:104405/1‑251 (MQ=255)
                                                                                                                     aGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                                                                                                                    >  2:233993/1‑179 (MQ=255)
                                                                                                                        tACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGATATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCAATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCt                                                                                                                         >  1:711119/1‑251 (MQ=255)
                                                                                                                                  ctctAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGACTCCTTTTTTCTTTTTCACTTCTGTTCACGCTg                                                                                                               >  2:146626/1‑251 (MQ=255)
                                                                                                                                    aaaaaggtgTATTTTACTTGCATTAAAAATGGATAAAAAACAACAT‑CACAGGTGTGTTTATTTTTTACAAAGAGGGGGGAAAAATGTAAGGACAGTGGGGGGGGGTTT‑ATTGTCGAAAAAAAAAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGa                                                                                                              <  1:506638/242‑1 (MQ=255)
                                                                                                                                              aTTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGACATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAAAAATGGCGCGATTCGTATGAAGCCCCTATGACAAGGATAAAAATAAACGAACGCCGAAGCTTCAGCTTATTGATTCCTTTTTTATTTTTCACTTCTGTTCACGCTGAACAAACGgctc                                                                                                   >  2:496264/1‑250 (MQ=255)
                                                                                                                                                    cATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGAAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTAAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCg                                                                                             >  2:590790/1‑251 (MQ=255)
                                                                                                                                                                         aGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGa                                                                          >  2:176586/1‑249 (MQ=255)
                                                                                                                                                                                                               gggagAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTCTTGATTCCTTTTTTCTTTTTCACTTCTGATCACGCTGAACAAACGGCTGATCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGGCCCGCAGCATCCCgctc                                    >  2:187209/5‑247 (MQ=255)
                                                                                                                                                                                                                                     gtAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACACGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCAcc             >  1:344974/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gggtgcgtttagtgataagAAAAAAAAAAAAAACCTCCATTGCAACAATGGCGCAATTCGGAGGAAGCCCCAATGACAAGGATAAAAATAACCGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTc           <  2:411889/233‑1 (MQ=255)
                                                                                                                                                                                                                                        ggTGCGGTT‑ATTGCCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCg          <  1:216784/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  aCTGTCGTGAAAAACAATAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAACTAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGCTCAATATCTCTCCTGGAAAGCCACCTCGGAACa     >  2:88082/1‑247 (MQ=255)
                                                                                                                                                                                                                                                  aCTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTc  >  1:613514/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       cGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttcttt                                                                                                                                  >  2:366702/1‑117 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
GATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAG‑TGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTT‑ACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCGAAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCGGAACAGTC  >  NC_000913/4287462‑4287951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑