| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,256,069 | 0 | G | C | 30.3% | 57.6 / 10.9 | 33 | P133R (CCG→CGG) | plsB | glycerol‑3‑phosphate 1‑O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (5/18); new base C (10/0); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCA > NC_000913/4255821‑4256309 | cccggtcccgggCAGGCAAAGGGGGGCCGCCGGCACCCGGACGTTGAGGGGCAAAGTGCATCCGCGCCCCGCGCCCCGTTTTCTGGGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCg < 2:460320/238‑1 (MQ=255) cGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACTTCGCCTTTTTTACACCCCgcaatcggg > 1:460320/1‑241 (MQ=255) cAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAAc < 1:501930/251‑1 (MQ=255) ggCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTC‑CTTTCCCCTTTTCTCGCCCCCGCGCGCGGCCAAACac > 1:560631/1‑250 (MQ=255) gTGGGCCTACGGAAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTACCCGTGCCGTTTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGac < 1:417068/251‑1 (MQ=255) ccGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑Ac < 1:548800/250‑1 (MQ=255) cAGCCAGACGTTGACGGGCAAAGGGCTTACGCGCCAGGCGCGCCAGTTTTTGAGCGATAGTTTTCTCCGTGCTGTGTTCATCCGCAATACGGCGCAGCGAAACTGCCGGAGAGAAACGCACAAAAC‑TGTCG‑CGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGTCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATc < 1:214028/251‑1 (MQ=255) aGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TGCACTCTGCCTTTTTTACGCCCCCCCGCGCGACCCAACATC‑aacacaactcgcacccacc > 1:603969/1‑233 (MQ=255) cGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACTTCGCCTTTTTTACGCCCCCGCGCGCGGCCACACATC‑ACCCACACTGGG‑CCCATCTGCCCAT‑c > 1:542693/1‑251 (MQ=255) aaGTGCATCCGCCCCCCGCCCGCCAGTTTCTGGGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAACCTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGtt < 1:279574/251‑1 (MQ=255) aGTGCATACGCGCCACGCGCGCCAGTTTCGGAGCGAGAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAATGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTg < 1:619746/251‑1 (MQ=255) aTACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCCTTTTTACCCCCCCGCGCGCGGCCAAACATC‑ACCGACACTGGC‑ACCCTCTGCACAC‑CCAGAATTTGGTTTgtacc > 1:280801/1‑247 (MQ=255) cACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACTTCGCCTTTTTTACGCCCCCGCGCGCGGCCAAACAAC‑ACCGACCCCGGC‑ACCCTCCGCCCAA‑CCCGA‑TTTTGGTTGCTACGGGGCaac > 1:384194/1‑249 (MQ=255) ccctgtttcggtgggagtttttgttCCGGGCCGTGTGCACCCGCCACACGGCGCGGCAAACCTGACGGCGAAAAACGCACAAACC‑GGGCG‑CGCCCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGT‑TTCACTTCGCCTTTTTCCCGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATa < 2:560631/227‑1 (MQ=255) gCGATAGTTTTATCCGGGCCGTGTTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGAACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAg < 1:289988/251‑1 (MQ=255) ttttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCAATTCGCCTTTTTTTCGCCCCCGCGCGGCACCAAAAATC‑ACCGACCCTGGCCACC‑TCTGCACCT‑CCAGA‑TTTTGGTTGCTACGGGGCCAATCGAGATAGTCGTGGGACAGCtttat > 1:109930/1‑247 (MQ=255) cccgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑GGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGActc < 1:405160/250‑1 (MQ=255) ggggcggtttccacccGCCATCCGGCGCAGCAAAACTGCCGGCGAGAAACG‑ATAAAACCTGTCG‑GGACCGAGCCACAGTACAGCGAAAAATTCCTTTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGCCCAAACAGC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtctt < 2:435554/237‑1 (MQ=255) cgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtctt < 1:35052/251‑1 (MQ=255) cggtgttaacccGCCATCCGGCCCAGGCAAACTGACGGGAAAAAACGCATAAACC‑GGTCT‑GGACCGAGCCACAGTGCAGCGAAAAATTTTTGTTCGCCGTTAACCATACGCGGCGGCGGG‑TTCACTGCGCCTGTTTCCCGCCCCGGCGCGCTACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCttt < 2:552000/241‑1 (MQ=255) cgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACTTCGCCTTTTTTACCCCCCCGCGCGCGACCCAAAAAC‑ACCCACACTCGC‑ACCCTCTTCACCTACCGAA‑TTTGGGTT‑CTACGGTGCAAAACGGGATAATCGTGGAACAGGTTAATAAACTCTTCtttt > 1:226786/1‑250 (MQ=255) cATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtg < 1:571738/251‑1 (MQ=255) ccccccaatcgccccAGCGAAACTGACGGCGA‑AAACCCAAAAAACCTGGCGGCGAC‑GAGCCCCAGTACAGCAAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTCCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTa < 2:384194/237‑1 (MQ=255) gcgcAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTTTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAaca < 1:617505/251‑1 (MQ=255) aCTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGccc < 1:542521/251‑1 (MQ=255) cGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCCGCGCGCGGCCAAACATC‑ACCCACACTGGC‑ACCCTCTGCCCAT‑CCAAA‑TTTTGGGTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAAAAGACTCTTCTTTTCGCGTGTTATAGGGGAACACACCCGGCccgccgcga > 1:196733/1‑247 (MQ=255) gAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑GTCACCTCCCCTTTTTTACGCCCCCGCCCGCGACCCAACATCCACC‑CCACCGGC‑ACCATCTGCACCA‑CCCGA‑TTTGGGGTGCTACCGGGCAAATCGAGATTGTCGTGGGACAGCTTAATATACTCTTCTTTTTGCGTGTAATATGGGAACACACGCGGCCCCCCGTGTATGACCACATAGCGGCGCAGTAGCgggg > 1:316326/1‑248 (MQ=255) cGAAAATTTTCTGTCCGCCGTTAACCATCCCCGGCGGCGGT‑TCCACTTCGCCTTTTTCCCGCCCCGGCGCGCGACAAAACACC‑ACCGACCCTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTCCGGTGCAAACCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTc < 1:517143/247‑1 (MQ=255) aaaaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTTCCCCCCCCGCGCGCGGCCAAACATC‑ACCCACACTTGC‑ACCCTCTGCACAT‑CCAGA‑TTTTGGGTGCTACGGTGCCAATCGAGATAATCGTGGGACAGCTTAATAGACTCTTCTTTTCGCGTGTAATAAGGGAACCCAACCGGCCCCCCCTGAATGAACAAATAACGCCGCAGTAGCGTGCCggcgggttcccgccg > 1:323645/1‑237 (MQ=255) gtcccccGTTAAGCAGCCGCGGGGGCGGG‑TTCCCTTCGCTTTTTCCACGCCCCGGCGCGCGACAAAACACC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTCCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACgg < 1:380893/245‑1 (MQ=255) tAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTTACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGGTGCTACGGGGCAAATCGAGATAGTCGTGGGACAGCTTAATAGACTCTTCTTTCTGCGGGGAATAGGTGAACACACGCGGCCCCCCGTGAATGAACACAAAGCGCGGCAGGAGCGTGCCGGCGGTTTCCAGCGGCCCCAACGGGTCAGGCa > 1:210154/1‑249 (MQ=255) cGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGAGCacc > 2:333055/1‑249 (MQ=255) cTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGgcgcg > 1:232697/1‑244 (MQ=255) tCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCa > 1:702848/1‑250 (MQ=255) cACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAtg > 2:388379/1‑220 (MQ=255) | GATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAAC‑TGTCG‑CGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGG‑TTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATC‑ACCGACACTGGC‑ACCATCTGCACAT‑CCAGA‑TTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCA > NC_000913/4255821‑4256309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |