New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4216100	52 (0.790)	3 (0.050)	3/464	5.4	4.5%	coding (623/1602 nt)	aceB	malate synthase A
	?	NC_000913	4216861 =	77 (1.220)					coding (1384/1602 nt)	aceB	malate synthase A
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4215858‑4216100 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcagtggATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGA  >  NC_000913/4216861‑4216900                                                                                                                                                                                                                                                                                             CAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCACTCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCCCCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCCCTGTTGGCAACGGGCAGTAGATCggca                                   >  2:165790/1‑246                                                                                                                      GGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGTACTGTTGGCAAAGGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGA  <  1:164153/166‑1                                                                                                                      GGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGTACTGTTGGCAAAGGGCAGTGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGA  >  2:164153/1‑166                                                                                                                                                                                                                                                                                             CAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTAACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCAGCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGTGGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAGGCACTGTTGGCAAAGGGCAGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4215858‑4216100 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcagtggATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGA  >  NC_000913/4216861‑4216900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.