Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,822,651	(CAGGAT)1→2	coding (252/2109 nt)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3822646 =	4 (0.060)	58 (0.890)	47/480	0.4	94.3%	coding (247/2109 nt)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
	?	NC_000913	= 3822651	3 (0.050)					coding (252/2109 nt)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT

GTTTCACGGGCGATGCGGCGACGTTTGTCCGGGCGAAGTGAGCCCAGCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3822884‑3822646 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGACGCTTGATTTGGTCTTCCGGCAGG‑TAGGTTTGAATCAGTTGATTCAGGCTTTCAAAC  <  NC_000913/3822651‑3822408                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       GTTTCAGGGGCGATGCGGCGACGGTTGGCCGGGCGAAGTGAGCCCAGCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGT                                                                                                                                                                                                                                           <  2:129251/251‑1       CGGGCGATGCGGCGGAGGTTGTCCGGGCGAAGTGAGCCCAGCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCCCCATCGCCATAATCATCTTGCGAAAGTTTTCGGGCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTTCCAGCTCGGCGAAGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGG                                                                                                                                                                                                                                     <  1:142599/251‑1            gtcGCGGCGACGTTTGTCCGGGCGAAGTGAGCCCAGCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTAT                                                                                                                                                                                                                                <  1:464929/248‑1            GATGCGGCGACGTTTGTCCGGGCGAAGTGAGCCCAGCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTAT                                                                                                                                                                                                                                >  1:466418/1‑251                gGGCGTCGTTTGGCCGGGCGAAGTGAGCCCAGCGGGCTCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTC                                                                                                                                                                                                                            <  2:694002/250‑1                                GGCGAAGTGAGCCCATCGGGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCCCCATCGCCATAATCATCTTGCGAAAGTTTTGGGCCTGCGCCT‑CTTTCTTATCGCGGAAATTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCACAAAGCTGTTCCATACCATGATCCTGGGAGGAGGCGGGAGGATCTTCAATCCCGTCCTGCAGC                                                                                                                                                                                                            >  2:611211/1‑251                                GGCGAAGTGAGCCCAGCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGC                                                                                                                                                                                                            >  1:573245/1‑251                                         aaCCCAGCGTGCGCATGTTGTGGGAACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTACGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACCCCCCCTCTACCAGCTCGGCGAAGCATTTACCAAAAAGCTGTTACATATCCTGATCCTGGTAGGTGGCAGGAGTATCTTAAATCcacaaatgcagcagcgccacc                                                                                                                                                                                                   >  2:634321/3‑230                                               gcggGCGCATGTTGTGGGTACGTCCGGCAAGTTTGATGAGGAGGACGCGGATATCCTGCACCATCGCCATAATCATCGTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTTCCAGCTCGGCGACGCGTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGTAGCGCCGTCATCAGC                                                                                                                                                                                             <  2:247671/247‑1                                               GCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGC                                                                                                                                                                                             <  1:440708/251‑1                                                        TGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCAT                                                                                                                                                                                      >  1:268414/1‑249                                                                      TCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGGGTATCTTCAATCACGACATGCAGCAGCGCCGCCATCAGCGTGTCATAGTCGCGTTTCATCTC                                                                                                                                                                      >  2:202841/1‑251                                                                           AAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGAAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCCTCTCGGCC                                                                                                                                                                  >  2:501375/1‑250                                                                                 GATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGC                                                                                                                                                           >  1:319066/1‑251                                                                                    gatGATGACGCGGATATCCTGCCCCATCGTCATAATCATCTTGCGAAAGTTTGCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTAGCAAGTTTCGACACCCCCTCTCCCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTGCATAGTCGAGTTTCATCTCGGCCAGAATGCAtg                                                                                                                                                        <  2:390586/248‑3                                                                                    GAGGATGACGCGGATAGCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAG                                                                                                                                                         <  2:175219/250‑1                                                                                       GATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGATTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATCGTCGAGTTTCATCTCGGCCAGAATGCAGGCA                                                                                                                                                      >  1:167119/1‑250                                                                                        agtACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCTTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAG                                                                                                                                                         <  2:562415/243‑1                                                                                        ATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAG                                                                                                                                                         >  1:562415/1‑246                                                                                                  tagcaTGCACCATCACCATAAACATCTTGCGAAAGTTTTCGGCCTGCGCCT‑ATTTCTTATCGCGGTACTTGAGTTTATCCAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGAAGGTGGCGGTAGTATCTTAAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCAAAGTCGAGTTTCATCTCGGCCCGAATTCAGGCACCCGCCACCGGG                                                                                                                                          >  2:486477/6‑251                                                                                                      CTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCG                                                                                                                                      >  1:60229/1‑251                                                                                                        GCACCATCGCAATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTG                                                                                                                                    >  1:311534/1‑251                                                                                                             ATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATA                                                                                                                               <  1:520774/251‑1                                                                                                                     AATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCAC                                                                                                                       >  1:455979/1‑251                                                                                                                           ccTGCGAAAGGTTTCGTCCTG‑GACTGCTTTATTATCGCGGAACTTGAGTGTAGCAAGTTTCGACTCCCCCTCTACCAGCGCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTGCAATCACGGCATGCACCAGCGCCGCCATCAGCGGTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCATCCGCTACCGGGGGCGTGATGTAGGGTTCACCGCTTG                                                                                                                 <  2:335514/249‑1                                                                                                                           CTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTG                                                                                                                 >  1:129435/1‑251                                                                                                                              GCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACCCCCCCTCTACCAGCGCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAAC                                                                                                              <  2:129435/251‑1                                                                                                                                      TTTCGGCCTGCGCCT‑CTGTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGATTATCTTCAAGCACGTCATGCAGCAGCGCCGCCAGCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTG                                                                                                       <  2:112493/250‑1                                                                                                                                        TCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTCCCGGGTGCGTGATATAGGGTTCACCGATTGAACGTGTTTGCCC                                                                                                    >  2:489984/1‑251                                                                                                                                         CGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCC                                                                                                   >  1:307693/1‑251                                                                                                                                           gccgGCGCCG‑CTTTCTTATCGGGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCGCGTCATGCAGCAGCGCCGCCACCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCC                                                                                                   <  2:353786/245‑1                                                                                                                                           GCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTC                                                                                                 >  1:372016/1‑251                                                                                                                                             CTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTCCCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAAC                                                                                                              <  2:250888/236‑1                                                                                                                                             CTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAAC                                                                                                              >  1:250888/1‑236                                                                                                                                              TGCGCCT‑CTATCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTCCCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGCGTATCTTCAATCCCGTCATGCAGCAGCGCCGCCATCAGCGGTTGATAGTCGAGTTTCATCTCGGCCATACAGCAGGCACCCGCTACCGGTTGCGTGCTATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTG                                                                                              >  2:78188/1‑251                                                                                                                                                CGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGAATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTG                                                                                              >  1:57349/1‑249                                                                                                                                                CGCCT‑CTTTCTTATCGCAGAAATAGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGACATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCACCCGCTACCGGGGGCGTGATATTGGGTGCACCCCTTAACCGTGTTTGCCCCTCGgg                                                                                              >  2:659052/1‑247                                                                                                                                                          CTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTG                                                                                   >  1:59616/1‑251                                                                                                                                                          CTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCAT                                                                                                                                                                         >  1:217288/1‑165                                                                                                                                                          CTTATCGCGGAACGTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCAT                                                                                                                                                                         <  2:217288/165‑1                                                                                                                                                                         GAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCT                                                                    <  1:144162/251‑1                                                                                                                                                                              TATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGCGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCCGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGGGCAACGAGATACGCCTGCCGC                                                               >  2:319380/1‑251                                                                                                                                                                              TATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCG                                                                <  1:218489/250‑1                                                                                                                                                                                tccAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGAGCAACGAGATACGCCTGCCGC                                                               >  2:339891/4‑249                                                                                                                                                                                  AAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATTTCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGAC                                                           <  1:339891/251‑1                                                                                                                                                                                        CGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGACGCTTG                                                      <  1:620123/250‑1                                                                                                                                                                                          ACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATCTCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTG                                                                                   >  2:356805/1‑219                                                                                                                                                                                          ACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTG                                                                                   <  1:356805/219‑1                                                                                                                                                                                                 CTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGACGCTTGATTTGGTCTT                                            >  1:223294/1‑251                                                                                                                                                                                                     ACCAGCTGGGGGACGCTTTTACCAAAAAGCTGTTCCATTTCCTGAGCCTGGTAGGGGGCGGGAGTATCTTCAATCACGTCATGCTGCAGCGCGGCCATCAGCGTTTCATAGTCGAGTTTCAGCGCGGCCAGAATGCAGGCAGCCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATCCGCCTGCCGCAGACGCTTGATTTGGTCTTCC                                          <  2:596400/249‑1                                                                                                                                                                                                     ACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGGGCAACGAGATACGCTTGCCGCAGACGCTTGATTTGGTCTTC                                           <  1:199093/248‑1                                                                                                                                                                                                       CAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGACGCTTGATTTGGTCTTCCGGC                                       >  1:642128/1‑250                                                                                                                                                                                                        cgaTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGCTCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAAGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGAGTTTGACCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGCCGCTTGATTTGGTCTTCCGGC                                       >  2:80367/4‑249                                                                                                                                                                                                                         ACCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGGGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCCACGAGATACGCCTGCCGCAGACGCTTGATTTGGACTTCCGGCAGG‑TAGGTTTGAATCAGTT                   >  2:600021/1‑251                                                                                                                                                                                                                          CCAAAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGACGCTTGATTTGGTCTTCCGGCAGG‑TAGGTTTGAATCAGTTG                  <  1:78188/251‑1                                                                                                                                                                                                                             AAAAGCTGTTCCATATCCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTACCCCTCGTGAGCATCACGTGCAACGGGATACGCCTGCCGCAGACGCTTGATTTGGTCTTCCG‑CAGGGTAGGTTTGAATCAGGTGATT               >  2:698444/1‑251                                                                                                                                                                                                                               AAGCTGTTCCATATCCTGACCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATACAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTCCCGAGTGCGTGCTATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAAACGCTTGATTTGGTCTTCCGGCAGG‑TAGGTTTGAATCAGTTGctttt             >  2:409452/1‑246                                                                                                                                                                                                                                             CCTGATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGACGCTTGATTTGGTCTTCCGGCAGG‑TAGGTTTGAATCAGTTGATTCAGGCTTTCAAAC  >  1:627424/1‑248                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       GTTTCACGGGCGATGCGGCGACGTTTGTCCGGGCGAAGTGAGCCCAGCGTGCGCATGTTGTGGGTACGGTCGGCAAGTTTGATGAGGATGACGCGGATATCCTGCACCATCGCCATAATCATCTTGCGAAAGTTTTCGGCCTGCGCCT‑CTTTCTTATCGCGGAACTTGAGTTTATCAAGTTTCGACACCCCCTCTACCAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3822884‑3822646 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTACCGGGTGCGTGATATAGGGTTCACCGCTTGAACGTGTTTGCCCCTCGTGAGCATCACGTGCAACGAGATACGCCTGCCGCAGACGCTTGATTTGGTCTTCCGGCAGG‑TAGGTTTGAATCAGTTGATTCAGGCTTTCAAAC  <  NC_000913/3822651‑3822408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.