New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1826504	56 (0.860)	3 (0.050)	3/464	5.4	4.0%	coding (420/969 nt)	astE	succinylglutamate desuccinylase
	?	NC_000913	1826847 =	91 (1.440)					coding (77/969 nt)	astE	succinylglutamate desuccinylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGTTCCTGATGGAACACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1826262‑1826504 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccagcgcCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTT  >  NC_000913/1826847‑1826886                                                                                                                                                                                                                                                                                             TCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGATCCTGATGGAACACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGACATTTCTCGTCCCAGGGACTGTCGCGTTGCGGTAATACACCGAACTGCGGCTGCAAGGAGCCACGAATTGCGGGATGTAGATCAAGGTGCCAGCGCCAagattc                                  >  2:595417/1‑245                                                                                                                                                     ATTTCTCGTCCCAGGGAATGTCGCGTTGCAGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTT  <  1:188085/135‑1                                                                                                                                                     ATTTCTCGTCCCAGGGAATGTCGCGTTGCAGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTT  >  2:188085/1‑135                                                                                                                                                                                                                                                                                             TCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCCAGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAACGTACCACCAGGTTCCTGATGGAACACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1826262‑1826504 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccagcgcCAGCGAACGCCGTTGATTTCTCGCTCGGTGATAACCGGTT  >  NC_000913/1826847‑1826886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.