Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,883	1	.	C	89.2%	182.5 / 15.6	65	coding (667/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (3/4);  new base C (29/29);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACC  >  NC_000913/3815636‑3816118                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            cgcgCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGGCGCTACAATGGATTCGA‑T‑T‑cc‑ccct                                                                                                                                                                                                                                            <  1:540522/250‑3 (MQ=255)    gcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCggg                                                                                                                                                                                                                                        <  1:672322/251‑1 (MQ=255)       cGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGcc                                                                                                                                                                                                                                      >  1:45121/1‑250 (MQ=255)               aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCcaac                                                                                                                                                                                                                             >  1:114555/1‑251 (MQ=255)                        gCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TACC‑CC‑TCGGGCCAGAGCCAACAAGATGAGt                                                                                                                                                                                                                    >  1:517082/1‑251 (MQ=255)                          gtggaagaaTTAGGGGCTTCTGCGCACGGATTTCAGCGTAAACTCCTCCAACTTTAACACCTGCTTGCTACGCGCAAATTCAAAAAGCTTGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCGACATACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCTTTCTGCGTCGCTAGAATGGATGCGA‑T‑TGCC‑CC‑TCGGTGCAGAGTCAACGAGATGAGTAg                                                                                                                                                                                                                  <  2:286942/248‑1 (MQ=255)                             gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TGTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                                                                                                                                                                                                <  2:632146/250‑1 (MQ=255)                               agaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAATCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACGGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                                                                                                                             <  1:469188/251‑1 (MQ=255)                                 aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACCCGCCAAACTTTAACTCCTGCTTTCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTGTG‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCTCCGCCTTCTGCGGCGCTACAATGGATTCGA‑TGC‑CC‑CC‑TCGGGCCAGAGCCAACAAGAGGAGTAGCTCTTCa                                                                                                                                                                                                           <  2:452710/251‑1 (MQ=255)                                      ggggCTTTTGCGCCCTGAATGCAGCGTTAACTCGCCAGACTGTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTT‑CTGTTTCTACAGTCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCGCTTCATGGGt                                                                                                                                                                                                      <  2:483535/251‑1 (MQ=255)                                       gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATGCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCACCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGTATTCTA‑T‑T‑CC‑CCCTCTGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                                                                                                                                                                                     <  1:415956/251‑1 (MQ=255)                                               ggacccggattgcctgttAAACGCGCCAAACTGTATCACCTGCTTGCTAAACGCAAATTCAATAAACGGGCGCGGATAGGGTTTCATGCTTTCGCTCCTCGTCTTACT‑TTTCTACAGACAAAAAAAAGGCGTCTCGTCAGTCGCCTTAAAAATCAGGTTGCCAGCGCCGCCTCCTGCGTTGCTGCAATGGATTCGT‑T‑TTCC‑GC‑TCGGGCCAGATCCAACAAGATGATTTGCTATTCATGGGTGAActgatc                                                                                                                                                                                             <  2:154980/234‑6 (MQ=255)                                               gcgcCCTGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTGAAAAATCAGGTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTTTTCATGGGTGAACGGCt                                                                                                                                                                                              <  2:302465/250‑1 (MQ=255)                                                 gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                                                                                                                           >  1:298064/1‑251 (MQ=255)                                                                tAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt                                                                                                                                                                            >  1:433759/1‑251 (MQ=255)                                                                   acttgtcaacctctAACACCAGCTTGCTAACCTCAAGTTCCATACACTGGCTCTGATATGTCTCCAGGCCCCCGATCCTCTTCTTACG‑TTTCTACATACAAATAACAGGCGACTCATCAGTCTCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTTCGGCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                                                                                                                                         <  2:133713/238‑1 (MQ=255)                                                                   aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTCCT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCTGGCCAGATCCAACAAGATGAGTAGCTCGTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                                                                                                                                         <  1:201022/251‑1 (MQ=255)                                                                              tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTACACTTCAatgatg                                                                                                                                                              >  1:672797/1‑251 (MQ=255)                                                                                     ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCACGt                                                                                                                                                       >  1:341700/1‑251 (MQ=255)                                                                                           ttctAAGCGATAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGTTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCTCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACCTCAATGATGCGCCAGTCTTc                                                                                                                                                   <  2:433759/247‑1 (MQ=255)                                                                                           tgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                                                                                                                                   <  1:49575/249‑1 (MQ=255)                                                                                                  cgcAAATTCAATAAACTTGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTGTGCCAGCTCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcataac                                                                                                                                          <  1:593954/251‑4 (MQ=255)                                                                                                   gcAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                                                                                                                                         >  1:258474/1‑251 (MQ=255)                                                                                                     aaaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGACGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTTCCGTCCCCTGCCCTTCAATGATGCCACCGTCTTCGGGCATCCCTAc                                                                                                                                       >  2:370045/1‑251 (MQ=255)                                                                                                      aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGATTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                                                                                                                                      <  1:619260/251‑1 (MQ=255)                                                                                                        ttCAATAAACTGGCGCTGCTATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGtt                                                                                                                                    >  1:183351/1‑251 (MQ=255)                                                                                                           aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCAt                                                                                                                                 <  1:520081/251‑1 (MQ=255)                                                                                                              aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                                                                                                                              <  1:501034/251‑1 (MQ=255)                                                                                                                     cgcTGAGATGGTGTCATGCCTTCGCTCCTCATCTTCCT‑TTTCTACAGACAAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTTCGTCGCTACAATGGACTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct                                                                                                                       <  2:194254/251‑1 (MQ=255)                                                                                                                               gTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAACAATCAGTTTGAAAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CCACC‑TCGGGCCAGAGCCAACAAGTTGAGTAGCTATTCATGGGTGCACGGCTCGCCTTCTGCCGTCCAATGCACTTCAATGATGCTCCCGTCATCGGTCATCACTACGTTCATGTCGGTCCCTGCGGCAGAGt                                                                                                             >  2:210667/1‑251 (MQ=255)                                                                                                                                 ttCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt                                                                                                           >  1:384482/1‑251 (MQ=255)                                                                                                                                  tCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCtt                                                                                                          >  1:543687/1‑251 (MQ=255)                                                                                                                                   cATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTc                                                                                                         <  1:15016/251‑1 (MQ=255)                                                                                                                                   cATGCCGTCGCTCCTCATCGTACT‑GTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCtt                                                                                                          <  1:2043/250‑1 (MQ=255)                                                                                                                                    aTGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTCCGTTCATGTCGGTCTCTGCGGCAGAGTCTTcc                                                                                                        >  2:636949/1‑250 (MQ=255)                                                                                                                                                ctcATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTcc                                                                                               >  1:186779/1‑248 (MQ=255)                                                                                                                                                            tttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGGGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGc                                                                                 >  1:386306/1‑251 (MQ=255)                                                                                                                                                             tgctccAGACAAAAAAAAGGCGGCTCAGCAGTCGCCTTAAAAATCAGTCTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCGCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGc                                                                                 <  2:18755/245‑1 (MQ=255)                                                                                                                                                                       aaaaaaaaGGCGACTCAGCAGTCGCCTTAAAAAACCGTTTGCCAGCGCCCCCTTCTTCGTCGCTACAATTGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCTAACAAGTTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCTGTCTCTGTGGCAGTGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTcg                                                                      <  2:426472/251‑2 (MQ=255)                                                                                                                                                                           aaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCGCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAAt                                                                  <  1:13009/251‑1 (MQ=255)                                                                                                                                                                              cggCGTCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTACGTCGCTACAGTGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGCGCCAACAAGATGAGTAGCTCTTCATGGGAGCACGGCTCGCCTTCTGCCATCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGTCCCCTGCGTCAGAGTCTTCAACGGTTTCCAGAACACCAACCGCTTCGCCATTCCAAATTcc                                                               >  2:205178/2‑251 (MQ=255)                                                                                                                                                                                    cTCATCAGTCGCCTTAACAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGATCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAc                                                            >  1:509459/1‑248 (MQ=255)                                                                                                                                                                                        tcaGTCGCCTTACAAATCAGTTTGCCAGCGCCGCCTTCTGCGTAGCTACAATGGATTCGA‑T‑TACC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                                                                                                                              >  2:443176/1‑114 (MQ=255)                                                                                                                                                                                        tcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCGGCGTCGCTACAATGGATTCGAGT‑C‑CC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                                                                                                                              <  1:443176/114‑1 (MQ=255)                                                                                                                                                                                           gTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCCTGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTCTTCCAGATCGCACACCGCTTCGCCGTTCCCCATTCCgcccacaactgcg                                                  >  2:402258/1‑239 (MQ=255)                                                                                                                                                                                            tctccTTAAAAATCAGGTGGCCAGCGCCGCCTTCTGCGTCGCTCCAATGGTTGCGA‑T‑T‑CC‑CCCGCGGGCCAGAGCCAACAAGATGAGTAGCTCTCCATGGGGGAACGGTTCGCCCCCGGCCGTCCCCTGCACTTCAAGTATGCGCCCGTCTTCGTTCACCACGACGTTCATGTCGGGCTCTGCGGCAGAGTCGTCAACGTATGCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCg                                                  <  2:341700/247‑1 (MQ=255)                                                                                                                                                                                             cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGCCAGAAACTGCGGc                                                >  2:613785/1‑251 (MQ=255)                                                                                                                                                                                                 ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAAAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACACAAACTGCGGCTAcc                                            >  1:679658/1‑251 (MQ=255)                                                                                                                                                                                                     aaaccAGTTTGCCAGCCCCGCCTACTGCGCCGCTACATGGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGAGGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCCCTACGTTCATGGCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATcc                                        <  2:590051/247‑1 (MQ=255)                                                                                                                                                                                                       aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCACTGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTc                                                                              >  1:644414/1‑211 (MQ=255)                                                                                                                                                                                                       aTCAGGTTGCCAGCGCCGTCTTCGGCGGCGCCACAATGGATTCGA‑T‑T‑CC‑CCCGCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTGCTGCCGTCCCCTGCACTTCAATTATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTc                                                                              <  2:644414/211‑1 (MQ=255)                                                                                                                                                                                                        tCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGCGCCAACAAGATGCGTCGCTCTTCATGGGGGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCCTTCCCAATTCCGCCAGAACCTGCGGCTACCATCCCtt                                     >  2:709190/1‑251 (MQ=255)                                                                                                                                                                                                         cAGTTTGCCAGGGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCttt                                    <  2:543687/251‑1 (MQ=255)                                                                                                                                                                                                         cAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCACCCCttt                                    >  1:296397/1‑251 (MQ=255)                                                                                                                                                                                                          aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCAA‑T‑T‑CC‑CCCTCGGGCCCGAGCCAACAAGATGAGTAGCTCTTCATGGGGGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCCTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTc                                   >  2:506762/1‑251 (MQ=255)                                                                                                                                                                                                             ttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCAt                                 <  1:613785/250‑1 (MQ=255)                                                                                                                                                                                                              tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGCGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTTCGGCTCCCCGCCCTTTCAttg                               >  2:194877/1‑249 (MQ=255)                                                                                                                                                                                                                  atcgccgcCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCGTCTGCCGTCCCCTACACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGAtt                           <  2:672797/249‑1 (MQ=255)                                                                                                                                                                                                                         ccTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTc                    >  1:563532/1‑251 (MQ=255)                                                                                                                                                                                                                          cTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAc                                                            >  2:297770/1‑210 (MQ=255)                                                                                                                                                                                                                          cTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAc                                                            <  1:297770/210‑1 (MQ=255)                                                                                                                                                                                                                            tCTGCGTCGCAACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAg                  <  2:114555/250‑1 (MQ=255)                                                                                                                                                                                                                             cTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCt                >  1:428471/1‑251 (MQ=255)                                                                                                                                                                                                                              tGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCtt               >  1:554102/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCTACAATGGATTCGA‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGtt         <  1:384571/251‑1 (MQ=255)                                                                                                                                                                                                                                           tGGATTCGC‑T‑T‑CC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGAACGAAACTGCGGCTACCATCCCTTTCCTCGGATTGGTTTTCAGCTTGCCGTTTTccacc  >  2:322777/1‑251 (MQ=255)                                                                                                                                                                                                                                                            cc‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCa                   >  1:104315/1‑220 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            CGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACT‑TTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CC‑CC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACC  >  NC_000913/3815636‑3816118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑