Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	160.9 / NA	48	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (23/25);  total (23/25)

TCAACGTGTTAGCAACGTTG‑AGATTGTT‑GGTGACAATGCGCAAATTGCTGTGAT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCC  >  NC_000913/3560219‑3560699                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     acaagttgtTAGCAACGTTG‑AGATTTTT‑GGTAACAATGCCAAAATTGCTGTGAT‑GGAGAATGGTGTGCGCTACCTCTCCCGG‑CGTGGTGCCGTTATCGAAAAACGGCGTCTAGCCATTGGGGTTTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                                                                                                                                         <  1:363216/245‑1 (MQ=255)     cGTGTTAGCAACGTTG‑AGATTGTT‑GGTGACAATGCGCAAATTGCTGTGAT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGcc                                                                                                                                                                                                                                     >  2:148682/1‑250 (MQ=255)       ggtTAGCA‑CGTTGGAGATTTTT‑GGTGACAATGCGCAAATTGCTGTGAT‑TGAGCAGGGCGTGCGCCCCCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCTTTGGGGTTTTG‑CCCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTcc                                                                                                                                                                                                                                   <  1:32673/250‑1 (MQ=255)         ttAGCAACGTGG‑AGATTGTG‑GGTGATAGTGCACAATTGGCGGTAATGTG‑GCAGGGCGTGCGATCCCG‑TTCCGGGCGGGGTGCCTATATCGAAAAACGGCGTGGAGCTTTTGGGTTTTTG‑CCCCGCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCGTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCg                                                                                                                                                                                                                                  <  2:290355/250‑1 (MQ=255)           agaaaCGTTG‑AG‑TTGTTTGGTGCAAAGGCGAAAATTCCTGTGAT‑TGGGCGGTGCGTGCGCTCCCGCTTCCGG‑CGTGGTGCCGATATGAATAAACAGGTTGGACCCTTTGGGGATTGG‑CTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCa                                                                                                                                                                                                                               <  1:200313/248‑1 (MQ=255)             cAACGTTG‑AGATTGTT‑GGTGACAATGCGCAAATTGCTGTGAT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                             >  2:357554/1‑251 (MQ=255)             cAACGTTG‑AGATTGTT‑GGTGACAATGCGCAAATTGCTGTGAT‑TGAGAAGTGCGTGCGCTACCGCTTCCGG‑CGTGGCGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGTGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGAAGCGCCGCCCCGCCCAGATGGCGCAGGATCCGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                             >  2:269930/1‑251 (MQ=255)              aaCGTTG‑AGATTGTT‑GGTGACAATGCGCAAATTGCTGTGAT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAACCAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGTTCGTGCCACGGCTTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGAAGGTTCAGGTTTTGCTccccacgcg                                                                                                                                                                                                                            >  2:661579/1‑244 (MQ=255)                  ttG‑AGATTGTT‑GGTGACACTGCGCAAATTGCTGTGCT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGCTTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGATGGCAGCGCCGCACCGCCATGATGGCGCAGGCTCAGGTTTTGCTACGCCAGCTCAtt                                                                                                                                                                                                                        >  2:103805/1‑251 (MQ=255)                           gtt‑gGTGACAATGCGCAAATTGCTGTGAT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                >  1:193743/1‑251 (MQ=255)                               ggTGACAATGCGCAAATTGCTGTGAT‑TGCGCAGTGCGTGCGCTCCCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTCgcggc                                                                                                                                                                                                             >  2:420884/1‑251 (MQ=255)                                         cgcAAATTGCTGTGAT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCCGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                                                                                                                                   >  1:140386/1‑251 (MQ=255)                                             aaattgttgttatggggCAGTGCGGGCCCTCCCGCTCCCGG‑GGTGGTCCCGATTCCGAAAAACAGCGTGGAGCCTTTGGGGTTTTG‑CTCCGCCACTTTGCGGACGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                                <  1:606278/235‑1 (MQ=255)                                                 gCTGTGAT‑TGAGCAGTGCTTGCGCTCCCGCTCCCGG‑CGTGGTCCCGATATCAATAAACAGCGCCGGGCCATTGGGGTTTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                                                            <  1:197105/250‑1 (MQ=255)                                                                     ggcgcTACCGCTCCCGG‑CGTGGTGCATATATCGATAAACAGCGTCGGGCC‑TTGGGGGTTTGGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                        <  1:651891/250‑1 (MQ=255)                                                                                ttCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                             >  1:125255/1‑251 (MQ=255)                                                                                           gtgCCGATATCGATAAACGGCGGGGGGCCATTGGGGATTGG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAcc                                                                                                                                                   <  1:148682/251‑1 (MQ=255)                                                                                                          aaCAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                      >  1:482935/1‑249 (MQ=255)                                                                                                           aCAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt                                                                                                                                   >  1:568238/1‑251 (MQ=255)                                                                                                                      catggggtgttttG‑CTCCGCCTCTTTGGGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCAGGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGTGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc                                                                                                                        <  2:357654/241‑1 (MQ=255)                                                                                                                        aTTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt                                                                                                                      >  1:142819/1‑251 (MQ=255)                                                                                                                         tgggggTTTTG‑CCCCGCCACTTTGCGGGCGATGCGCTCTTTGTCTTCGGTCTGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt                                                                                                                      <  1:128979/248‑1 (MQ=255)                                                                                                                          gggggtgttgctcccggcaatttGCGTGCGCTGCGTTGTTTTTTTTCGGTGAGGGTGGCCTGGCGATCGTGCCACGGGGTGTAACCCGACGTGGAAGGCAGCCACCCCCCGCCATGATGGCGCAGGATCAGGTTGTGCGCCGCAAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAGAACCCTGCTGTTTAAACAGTTCGATAATACCgg                                                                                                                      <  2:494338/230‑2 (MQ=255)                                                                                                                           ggggATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGACTAGTCTGCGGGCTGACGGGGAAATGCTCTCCCAGCTCTTCGGTACTGACTTAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                                   >  2:10892/1‑251 (MQ=255)                                                                                                                                      tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTgtgt                                                                                                         >  2:335511/1‑251 (MQ=255)                                                                                                                                      cctgccttttgtgtgtgtgggcgcTCTTTGTTTTGGTTCTGGGTGGCCTGGCGTCCGCGCCACGGTGTGTTACCCGAATGGGAAGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                                           <  2:399391/229‑1 (MQ=255)                                                                                                                                       ccGCCACTTTGCGGGCGATGCGCTTTTTTCCTGCGGTCGGGGTGGCCTTGCGACCGTGCCACGGCGTTTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtt                                                                                                        <  1:283462/251‑1 (MQ=255)                                                                                                                                        cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt                                                                                                       >  2:460731/1‑251 (MQ=255)                                                                                                                                                ggCGGGCGATGCTCTCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTATCCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCTATAAGACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                               <  1:569287/250‑1 (MQ=255)                                                                                                                                                       gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                                                        >  1:21537/1‑251 (MQ=255)                                                                                                                                                          gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                                                                                                                                <  1:480536/192‑1 (MQ=255)                                                                                                                                                          gcgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAACctg                                                                                                                                                >  2:480536/1‑192 (MQ=255)                                                                                                                                                              tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACAGAACTTGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTgg                                                                                  >  1:526979/1‑250 (MQ=255)                                                                                                                                                                             gggggggcgttGCGATCGGGCCAGGGGGGGTGAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                                  <  2:142819/242‑1 (MQ=255)                                                                                                                                                                              ggggggcctcgcgtgcGTGCCACGGGGTGGTACCCGAACTGGAGGGCGGCCCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                                 <  2:568238/236‑1 (MQ=255)                                                                                                                                                                                    ccttgcgcgtgTGCCACGGCTTGTTACCCAAACGTGAAGGCGGCGCCGCACCTCTATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                                           <  1:61867/241‑1 (MQ=255)                                                                                                                                                                                       tGCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTg                                                          <  1:460731/249‑1 (MQ=255)                                                                                                                                                                                        gCGATCGGGCCAGGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGc                                                       <  1:235723/251‑1 (MQ=255)                                                                                                                                                                                        gCGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAg                                                        <  1:357554/250‑1 (MQ=255)                                                                                                                                                                                                    gggggtgtTAACCGAACGGGAAGGCAGCGCCGCTCCGCCATGATGGCGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATTCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                                           <  1:103805/247‑1 (MQ=255)                                                                                                                                                                                                        gtgtTACCCGATCTGGAAGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTGGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa                                       <  1:335511/251‑1 (MQ=255)                                                                                                                                                                                                        gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaaa                                                                                         >  1:217574/1‑201 (MQ=255)                                                                                                                                                                                                               ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa                                 >  1:159571/1‑250 (MQ=255)                                                                                                                                                                                                                  aaCTGGAAGGCCGCGCCTCACCGCCAGCATGGCGCAGGATCTGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGTGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAGCAACAAAAGCCATCGCTAAAcc                             <  2:475598/251‑1 (MQ=255)                                                                                                                                                                                                                   aCTGGCAGGCAGCGACGCACCGCCATAATGCCGCAGGATCAGGTTTAGCTCAGCCAGCACATTGAGGACGAGGCGAATAGTCTGCGGGCTGACGGCGAAATGCTATACCAGCTCTTCGGTACTGACATAACACTGATGTATAACCAGATCGATAATACCGTTGTGACGTTGTATCTGTTTCATTTATAATGCACTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCTACAACAGCCACAGCTAAAcac                            >  2:426334/1‑249 (MQ=255)                                                                                                                                                                                                                    cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc                            >  1:606146/1‑250 (MQ=255)                                                                                                                                                                                                                                       cGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGCtgtg        >  2:252163/1‑251 (MQ=255)                                                                                                                                                                                                                                             gatgGCGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTGCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTcc  <  2:193743/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     TCAACGTGTTAGCAACGTTG‑AGATTGTT‑GGTGACAATGCGCAAATTGCTGTGAT‑TGAGCAGTGCGTGCGCTACCGCTTCCGG‑CGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCC  >  NC_000913/3560219‑3560699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑