| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,917,088 | 0 | G | C | 22.9% | 171.7 / 11.8 | 71 | A678P (GCT→CCT) | barA | sensor histidine kinase BarA |
| Reads supporting (aligned to +/- strand): ref base G (8/46); new base C (16/0); total (24/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATTAGCGAAAGCGGTATCGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGG > NC_000913/2916848‑2917338 | cGATTAGCGAAAGCGGTATCGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑GTGACAAACCCCCTCACCTGa > 1:760613/1‑250 (MQ=255) ttAGCGAAAGCGGTATCGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGGTG‑TTTACAACCCCCCTCAACTGAAACt > 1:259056/1‑251 (MQ=255) aGCGAAAGCGGTACCGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGGAAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTTCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTa < 2:679379/251‑1 (MQ=255) gAAAGCGGTATCGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCGACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCg < 1:433210/251‑1 (MQ=255) cGGTATCGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTT‑ATTAAAACCCCCCCAACCTGAAAATTATCAgcgcc > 1:144861/1‑250 (MQ=255) gTATCGATGACCGATTTCCTGATGGTGGCACTTTCTTGCTATGCACAAGTCAATGCTGAAAAACTCAAGCAAGTTGGTAGCGGCGCGTGTCTGCTGAAACCATTAACTCCTACACGCCTGTGGCCTGCCCTGACGGAAGTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGGAAGCTGGCAATGACAGTTATGGCGGTTG‑ATGACAACCACGCTAACCTGAAACTTATCGGCGCAtt < 1:779734/251‑1 (MQ=255) cGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑t < 1:727257/250‑1 (MQ=255) gACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGAGGTTG‑ATGACAAACCCGCCAAACCGAAAACTATCGGGGCATTTC‑Tggg > 1:186772/1‑248 (MQ=255) gACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCAACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGACAGTAAGCTGGCAATGCCAGTCATGGCGGTTG‑TTGAAAACCCCCCCCACCTGaaaattttgcggccgtttgtggg > 2:76715/1‑229 (MQ=255) aCCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGa < 1:767077/251‑1 (MQ=255) gATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATTACAACCCCCCTCAACTGAAACTTATTGGGGCAATGC‑TGGGAGatat > 1:88664/1‑251 (MQ=255) tttCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATg < 2:88664/250‑1 (MQ=255) gactcTGGCACTTCCTTGCCATGCACAAGTCAATTCATAAAAACGCAATCCGGATGGTAATGGAGCGTGACTGCGGAAACCAATAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTTCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCTCGCTAACCTGAAACTTATCGGCGCATTGA‑TGGAAGATATGGTGC‑aa < 2:757599/246‑1 (MQ=255) gATGCTGTCACTTCCTTGCCATGCACAAGACAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AAc < 2:149460/251‑1 (MQ=255) aTGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGAAAACCCCGCTAACCTGAAACTTATCGGCGCATTGCATGGaaaaatggggcaac > 1:831878/1‑238 (MQ=255) gcgggCACTTCCTTGCCATGCACAAGTCAATGCGGAAAAACTCAAGCAAGAGGGTATCGGCGCGTGTCTGCTGAAACCAGTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTGATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAt‑g < 2:103857/248‑1 (MQ=255) ggCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑Tgg < 1:788545/251‑1 (MQ=255) aagaacaAGTCAATGCTGAAAAACTCAATCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACTCCTGTTGCCTGCCCTTACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATTAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGc < 2:252842/247‑1 (MQ=255) aaGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCAt < 2:73519/251‑1 (MQ=255) aGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTAATCACAAAAAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑GTGACAACCCCCCCAACCCGAAAATTATCGGCGCAATGC‑TGGGAAATTAGGTGG‑AACAT‑G‑TGG‑AAACTTGC‑GAGAGAGGGCATc > 1:127268/1‑251 (MQ=255) gTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCCCTAACCTGAAACTTATTGGCGCATTTC‑TTGAAGGAATGTGGC‑AAAAT‑G‑TGG‑GACttttccataacggggatc > 1:11684/1‑235 (MQ=255) cAATGCTGAAAAACTCAAGCAAGAGGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAgg < 2:216949/251‑1 (MQ=255) gAAAAACTCCACCAAGATGGTATCGGCGCGTGTCTGCAGAAACCATTAACCCCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTg < 2:162121/250‑1 (MQ=255) aaaaaCTCAAGCAAGAGGGTATCGGCGCGTGTCTGCGGAAACCATTAACACCTACACGCCTGGTGCCGGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAGCGCCGCTAAGCTGAAACGTAGCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGaa < 1:90557/251‑1 (MQ=255) caatcaagATGGTATCGGCCCGTGGCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTTCCCTGACGGAATTTGGTCATCACAAACAAAACACGCTTGTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCAGTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGc < 2:63162/245‑1 (MQ=255) caagcaagATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGc < 1:521291/249‑1 (MQ=255) caagcaagATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCCCTAAACCGAAACTTATCGGCGCATTGC‑TGGGAGATATGGGGC‑AAACT‑G‑TGG‑GACTTTGC‑GATAGCGGGCATCAGGCGGGTGGACGGGCgg > 1:120422/1‑250 (MQ=255) aagATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCa < 2:330371/226‑1 (MQ=255) ggTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑GTGACAACCCCCCTAACCTGAAAATTATCGGGGCCTTGC‑TGGGAGATATGGTGC‑AAAATTG‑GGG‑AA‑TTTGC‑GATAGCGGGCATAAGGGGGGTGTACggggggaaaacattgc > 1:138478/1‑238 (MQ=255) aTCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGt < 1:468499/250‑1 (MQ=255) ggCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATc < 1:445128/162‑1 (MQ=255) cgccggtCTGCTGAACCCATAAACACCTACACGCCTGTTGCCTGCCCGGACGGAATTTTTTCAACACAAACAAAACACGCCTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGATAACCCCGCTAGCCTGAAACTTATCGGCGCATGGC‑TGGAAGATAGGGTGC‑AACAT‑G‑TGG‑AACGGTGC‑GATAGCGGGCATCAGGCGGTTGAACGGTCGAAACTGATGCCGTTCGAtt < 2:339586/246‑1 (MQ=255) cgttcctgctgAAACCAGTAACTCCCTCACGCCTGTCTCCTGCCCTGTCGGAATTTTGTCATCACAAACCAAACACGCTTTTGCCTGTATCCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACATCCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGAGATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGAGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGAtt < 2:482839/244‑1 (MQ=255) gtCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGAt < 1:368630/250‑1 (MQ=255) tgctgAAACCATTAACACCTACACGCCTGTTGCCTACCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGa < 1:103282/166‑1 (MQ=255) gcttaaaCCAATAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTGGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCTAAACAGATGCCGTTCGATTTGATCTTa < 2:738094/246‑1 (MQ=255) gctgAAACCATTAACACCTACACGCCGGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTa < 2:73295/250‑1 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑GTGGCAACCCCCCTAACCTGAAACTTATCGGCGCCTTTG‑TGGAAGGTATTGTGA‑AACAA‑G‑TGGGAACTTTG‑‑GGTTGCGGGGATCAGGGGGGTGTAAGGGCGCAACAGaagacctttggttttattttaatgg > 1:861415/1‑227 (MQ=255) gAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAAt < 1:62070/249‑1 (MQ=255) ttAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCCCCAACCTGAAAATTATCGGCGCATTGC‑TGGAAGATATGGGGC‑AACAT‑GGTGG‑A‑CTTTGC‑GATAGCGGGCATCAGGCGGGTTAACGGGCGGAACAGATGCCGTTCGATTTTGTCTTTATGGATAtta > 1:738918/1‑249 (MQ=255) acCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGGCAACCCCCCCAACCTGAAACTTATTGGCGCATTTC‑TGGAAGATATGGTGCCAAAAT‑‑‑TGG‑GACTTTTC‑GTTAGCGGGCATCCAGCGGTTGAACGGGCGGAACAGATGCCCTTTGATTTTATCTTAAATGATAttttaattc > 1:717047/1‑244 (MQ=255) acCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACg < 2:823600/204‑1 (MQ=255) acCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAAACCCCCTAACCCTAAACTTATCGGCGCATTTC‑TGGGAGATATTGGGC‑AACAT‑G‑TGG‑GACTTTTC‑GATAGCGGGGCTCAAGCGGTTGAACg > 1:823600/1‑204 (MQ=255) cacacGCCTGTTGCTTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCgg < 2:711084/250‑1 (MQ=255) cTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTTTAT‑ACAACCCCCATAACCCTAAAATTATCGGCGCACTGC‑TTGAATATATGGGGC‑AACAT‑A‑TGG‑AACATTTC‑GCTTTCGGGCATAAGGCGGTTTAAAGGGCGAAAGAGATGCCGGTCGATATGTTCTTTATTGGTATTCacatcacggtcagagg > 2:288087/1‑236 (MQ=255) cTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGATG‑ATGAAAAACCCGCTAACCTGAAACTTATCGGCGGATTGC‑TTGAAGAAATGGGGC‑AAAAT‑G‑TGG‑AACTTTTC‑GATAGGGGGGATCAGGCGGGTGAAAGGGCGAAAAAGATGACGTTTGGTTTTATCTTAATGGGTATTCAAATGACGGACa‑tggg > 1:601296/1‑250 (MQ=255) tGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑TTGACAACCCCCCCAACCTGCAACTTATTGGCGCAATGC‑TGGAAGGAATGGTGC‑AACAT‑T‑TGG‑GACTTTGC‑GCTAACCGGCATCCGGCGGGTGGAAGGGCGGAACAAATGCCGGTCGGTTTGCTCTTAATAGATATTTAAATGCCGGACa‑aggat > 1:518208/1‑251 (MQ=255) gccCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTAACGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCg < 1:332217/251‑1 (MQ=255) tGACGGAATTTTTTCATCACAAACAAAACTCGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAATCTTATCGGCGCATTGC‑TGTAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TTTATGGCATTCggg < 2:747442/249‑1 (MQ=255) gACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCt < 1:458455/251‑1 (MQ=255) gAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGa < 1:302902/251‑1 (MQ=255) ttGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTc < 1:5235/250‑1 (MQ=255) atcaCAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATccac < 2:120422/251‑1 (MQ=255) aaacaaaacaCGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATGCGGGCCTGCGAACTCATCCACCAA‑Ct < 2:760613/251‑1 (MQ=255) caaaacaCGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑GTTACAACCCCCCTCACCCGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AGCTTTGCCAATA‑CGGGCATCAGGCGGTTGAACAGGCGAAACAGAGGCCGTGCGATTTGTGCTTTATGGGAATTCAAAAGCCGGACA‑TTGATGGCATTCGTGCCCGCGCACTTAACTACCAA‑CTccc > 1:201378/1‑251 (MQ=255) gtGCCTGTAACCGAGGAAAGTACGCTTGCAATGACAGTCATGGTGGTTG‑ATGACATCCCCGCTACCCTTAAACTTATCGGCTCATTGC‑TGGAAGATATGGTGC‑AACTT‑G‑TGG‑AACTTTGC‑GATTGCGGGCATCAGGCGGTTTAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGTATATTCAAACGCCTGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCAGCAGcaac < 2:738176/249‑1 (MQ=255) ccTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAAc < 1:239839/251‑1 (MQ=255) aTGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATACGCGCCTTAC‑TGGAAGATATGGTGC‑AACAA‑T‑TGG‑AACTTTGC‑GATAGAGGGCATCCAGCGGGTGAAAGGAGGAAAAAAAGGACGTTCTATTTGAGTTTTATGGATATTAAAATTCACGACAATGG‑TGGGATTCGGGCCTGCGGACTCATCCACCAAACTccccccaagcacaaaaagccgggtttc > 1:802033/1‑227 (MQ=255) gAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATcgc < 1:165076/251‑1 (MQ=255) cAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCAt < 1:76715/251‑1 (MQ=255) aaTGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATTGATATTCAAATGCCGGACA‑TGGATGGCAGTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATg < 1:849243/251‑1 (MQ=255) aTGACAGTCATGGCGGCTG‑ATGACAACCCCGCTAACCTGAAACTTAGCGTCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGc < 2:738918/251‑1 (MQ=255) aTGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGc < 1:292319/180‑1 (MQ=255) cGGTTG‑ATGACAACCCCTCTAACCGGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCaa < 2:186772/251‑1 (MQ=255) ggTTG‑ATGACATCCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GGTAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCAATCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGc < 2:534783/203‑1 (MQ=255) ggTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGc > 1:534783/1‑203 (MQ=255) tg‑atgaCAACCCCGCTAACCTGAAACCTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑GGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCaaaaa < 2:259056/250‑1 (MQ=255) tgaCAAACCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACTCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAagcg > 1:417618/1‑248 (MQ=255) aaCCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCCTGCAATGGCCGGGCAAAAAGAGAAgct > 2:854023/1‑251 (MQ=255) ccGCTCACCTGAATCTTGTCGGCGCATTGC‑TGGAAGATATGGTGC‑AAAAT‑G‑TGG‑AAATTTGC‑GATAGCGGGCAGCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCAGTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATTCAATGGTCGGGCAAAAAGAGAAGCTGCtt < 2:717047/251‑1 (MQ=255) cGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTg > 1:266515/1‑251 (MQ=255) gCTAACCTGAAACTTAACGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTgg < 2:619289/251‑1 (MQ=255) | CGATTAGCGAAAGCGGTATCGATGACCGATTTCCTGATGCTGGCACTTCCTTGCCATGCACAAGTCAATGCTGAAAAACTCAAGCAAGATGGTATCGGCGCGTGTCTGCTGAAACCATTAACACCTACACGCCTGTTGCCTGCCCTGACGGAATTTTGTCATCACAAACAAAACACGCTTTTGCCTGTAACCGATGAAAGTAAGCTGGCAATGACAGTCATGGCGGTTG‑ATGACAACCCCGCTAACCTGAAACTTATCGGCGCATTGC‑TGGAAGATATGGTGC‑AACAT‑G‑TGG‑AACTTTGC‑GATAGCGGGCATCAGGCGGTTGAACGGGCGAAACAGATGCCGTTCGATTTGATCTTAATGGATATTCAAATGCCGGACA‑TGGATGGCATTCGGGCCTGCGAACTCATCCACCAA‑CTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGG > NC_000913/2916848‑2917338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |