| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 388,336 | 0 | A | C | 32.1% | 79.2 / 10.1 | 54 | N181T (AAC→ACC) | tauD | taurine dioxygenase |
| Reads supporting (aligned to +/- strand): ref base A (26/10); new base C (1/16); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATACCGATGTGACATTTATTGAAACGCCACCCGCAGGGGCGATTCTGGCAGCTAAAGAGTTACCTTCGACCGGCGGTGATACGCTCTGGACCAGCGGTA‑TTGCGGCCTATGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGC > NC_000913/388088‑388581 | gCATACCGATGTGACATTTATTGAAACGCCACCCGCAGGGGCGATTCTGGCAGCTAAAGAGTTACCTTCGACCGGCGGTGATACGCTCTGGACCAGCGGTA‑TTGCGGCCTATGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAAcc < 1:697914/251‑1 (MQ=255) tgacccGCCACCCTGAGGGGCGATTCTGGAAGCAAAAGAGTTCCTTTCGACGGGGGGTGATACGCTCTGGACCAGCGGTA‑TTGGGGCCTATGAGGCGCTCTCTGTTCCC‑TCCCGCCAGCTGC‑TGAGTGGGC‑TGCTGGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAATTCCCGCAAACCCGAGGAGAAACATCACCGCTGGCGCGAGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCggtg < 1:852578/245‑1 (MQ=255) ggggggggCGATGCGGGCAGATAAAGATTTACTTTCGCCGGGCGGTTATCCGCTCTGGACCAGGGGTA‑TTGGGGCTAAGGGGGCG‑TCTTCGTTCCCCTCCCGCCGGCTGC‑TGAGTGGGC‑TGCGGGCGGAGCTGGATTTCCGAAAATCGTTCCCGGAATA‑CAAATCCCTCAAAACCGAGGAGAAACACCAACGCGGGCGGGGGGCGTT‑CGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATc < 2:772673/247‑1 (MQ=255) gggCAGCTAAAGAGTTACCTCCGACCGGCGGTGATACGCTCTGGACCAGCGGTA‑TTGCGGCCTATGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGC‑GGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTaaa < 1:319173/250‑1 (MQ=255) cagttAAAGAGTTCCCTTCGACCGGCGGTGATCCGCTCGGGACCAGCGGTA‑TTGCGGCCTAGGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGT‑TGAGTGGGT‑TGCGTGCGGAGAATGATTTCCGTAAATCGTTCCCGAAATA‑AAAATACCGCAAAACCGAGGAGGAACATCACCGCGGGCGCGAGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAg < 1:62656/247‑1 (MQ=255) agTTACCTTCGACCGGCGGTGATACGCTCTGGACCCGCGGTA‑TTGCGGCCTCTGGGGCGCTCTCTGTTCCC‑TTCCGCCCGCTGC‑TGGGTGGGC‑TGCGTGCGGGGCATGATTTCCGTAAATCGTTCCCCGAATA‑CAAATACCGCAAAACCGAGGAGGGACATCCACGCTGGCGCGGGGCGGG‑CGCGGAAAACCCGCCGGTGCTACATCCGGGGGTGCGAACGCACCCCGTGGGCGGGAAAAAggaccggttt > 1:573997/1‑243 (MQ=255) tttacctgcgacaggcgtggaagcGCTCTGGACCAGCG‑TAGTTGCGGCCTATGAGGCGCTCTGTGTTCCC‑TTCCGCC‑TATGCCTAAGTGGGC‑TGCTTGCTGAGCATGTTATCCGTAAATCGTTCCCGGAATA‑CAAATCCCGCAAAACCGAGGAGGAACATAATCTCTGGCGCGGGGCGGT‑CGCGAAAAGCCCGCCGTTGCTTCAGCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGttt < 1:251496/227‑1 (MQ=255) gACCGGCGGTGATACGTTCTGGACCAGCGGTT‑TTGCGGCCTATGATGCGTTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCTTGCGGAGCATAATTTCCGAAAATCTTTCCCGGAATA‑CAAATACCGCAAACCCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAg < 1:36340/251‑1 (MQ=255) cggcggTGATACGCTCTGGACCAGCGGTA‑TTGCGGCCTATGAGGCGCTCGCTGTTCCC‑TCCCGCCAGCTGC‑TGGGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑AAAATACCGCAAACCCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCt < 1:803366/251‑1 (MQ=255) ggtgatcctctctgaccctgctgTA‑TTGCGTCTTATGGGGCTCTCTCTGTCCCC‑TTCCGCCAGCTGC‑TGAGGGGGC‑GGCTTGCGGAGCATCATTTCCGAAAATCGTCCCCGTAAAA‑CAAATACCGCAAAACTGAGGAGAAACATCACCCCGGTCGCGGGGCGTT‑CGCGAAAAACCCGCCGTTGCTACATCCGGGGGTGCGAACGCATCCGGTGAGCGGTAAACAGTCGCTGTTTGTGAATGAAGGCTTtac < 1:266618/229‑1 (MQ=255) atccGCTCGGGACCGGCGGTA‑GGGCGGCTTAGGAGGCGCTCTCTGTCCCC‑TCCCGCCATCGGC‑TGAG‑GGGCGTGCGGGCGGAGCATGATTCCCGAAAATCGTTCCCGAAATA‑AAAATACCGCAAAACCGAGGGGGAACATCAACGCGGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTAcg < 1:45459/248‑1 (MQ=255) tACGCTCTGGACCAGCGGTA‑TTGCGGCCTATGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTAcgc > 1:180481/1‑251 (MQ=255) taCCAGCGGTA‑TTGCGGCCTATGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAAttgttg > 1:266449/2‑251 (MQ=255) cTATGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGTAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAagagg > 1:101345/1‑250 (MQ=255) gggCGCTCTCTGTCCCC‑TCCCGCCAGCTGC‑TGAGGGGGC‑TGCGTGCGGAGCATGATTCCCGAAAATCGTCCCCGGAATA‑CAAATCCCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGaa < 1:191226/250‑1 (MQ=255) cgcTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAAACCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTCCGCGAATTGGTGATGTGAGCGGGCAAGGGAGCGAAGcc > 2:253447/1‑251 (MQ=255) ctGTTCCC‑TTCCGCCAGCTGC‑TGGGTGGGC‑GGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTCGCGCGAGGAAGT‑CGCGAGAAACCCGCCGTTGCTACAACAGGGGGTGCGACCGCATCCGGTGAACGGTAAACAGGCGCTATTTGTGAATGAAGGCTTTACTCCGCGCATTGTTGATGTGAGCGGGAAAGAGAGCGCAGCCttttt > 2:745197/1‑247 (MQ=255) gtcccc‑TTCCGCCAGTTGC‑TGAGTGGGC‑TCGGTGCGGAGCATTATTTCCGTAAATCCTTCCCGGAATA‑CAAATCCCGAAAAACCGGGGAGGAACATCAGCCCGGGGGCGGGGCGGC‑CGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAg < 1:442972/248‑1 (MQ=255) tcccattCCGCCAGCTGT‑TGAGTGGGC‑TGCGGGCGGAGCATGATTTCCGTAAACCGTTCCCGGAAAA‑AAAATCCCGAAAAGCCGAGGAGGAACACCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAg < 1:204432/245‑1 (MQ=255) gCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTtttgttt > 1:782375/1‑251 (MQ=255) gCCAGCTGC‑TGAGGGGGC‑TGCGTGCGGAGCATGATTTCCGTAAACCGTTCCCGGAATA‑CAAATACCGAAAACCCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTtttgttt < 1:204745/251‑1 (MQ=255) gc‑tgAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCat > 1:295861/1‑250 (MQ=255) cggAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCACCGCTGGCGCGGGGCGGT‑CGCAAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCatat < 1:121723/249‑1 (MQ=255) tgAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAGTA‑CAACTACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGGGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATc > 2:213583/1‑251 (MQ=255) tgAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATc > 1:572062/1‑251 (MQ=255) cgcggggtagacATGATTTCCGTAAAGC‑TTCCCGGAAAAACAAACCCGGCAAAACCGGGGAGGAACATCAAGGCGGGGGCGGGGCGGT‑GGGGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACGGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCg < 2:87287/240‑1 (MQ=255) gcgGGGCATGATTTCGGAAATTCGTTCCCGGAATA‑CAAATCCCGCAAAACCGAGGAGGAACACCAACGCTGGCGCGAGGCGTT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGtt < 1:845246/251‑1 (MQ=255) gAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGCGTTTc > 1:505298/1‑250 (MQ=255) cATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGCGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCCTATCA‑CCAAACCGGAGTTTCAggg > 2:384071/1‑250 (MQ=255) cATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGGTTCAGGt > 2:771409/1‑251 (MQ=255) gATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTgcg > 1:130585/1‑251 (MQ=255) gATTTCCGTAAATCGTTCCCGAACTA‑CAAATACCGCAAAACCGAGGAGGAACATAAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATACGGTGGTGCGCACGCCTCCGGTGAGCGGTACACAGGCGCTGTTTGTGAATGAAGGATTTCCTACGCGAATTGTTGATGTGCGCGAGAAAGAGAGCGACGCCTTGTTAAGTTTTTTGTTTGCCCATATAA‑CCCACCCGGAGTTTCAGGTgcg > 2:809555/1‑251 (MQ=255) ttCCGTAAATCGTTCCCGAAATA‑CAAATACCGAAAAACCGAGGAGGAACATCATCGCGGGCGCGGGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTgcgct < 1:424401/250‑1 (MQ=255) tCCGAAAATCTTTCCCGGAATA‑AAAATCCCGCAAACCCGGGGAGGAACATCAGCGCTGGCGCGAGGCGGC‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTgcgctg < 1:653957/250‑1 (MQ=255) cgaaaagcttgcctgaaaTA‑AAAATACCGAAAAACCGAGGATGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTTGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTgcgctggcg < 1:521066/235‑1 (MQ=255) aaTCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCTCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGcgctggcgctgg > 1:268206/1‑251 (MQ=255) aTCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACACGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGCGTTTCAGGTgcgctggcgctagc > 2:258697/1‑248 (MQ=255) ggAATA‑CAAATACCGCAAAACCGAGGAGGCACCTCAACGCTGGCGCGAGGCGGA‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGCACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGACTTGTTGATGTGAGAGAGAAAGAGAGCGAAGCCTTCTTAAGTTTTTTGTTTGCTCAGATCA‑CCAAACCGGAGTTTCAAGTGCGCTGGCGCTGGCCACCACATg > 2:38508/1‑251 (MQ=255) aaTA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGat > 1:412812/1‑251 (MQ=255) aCCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAACAACCCGCCGTTGCTACATCCGGTGGTGCGGACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGATAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCAATATTA‑CCAAACCGGAGTTTCAGGAGCGCAGGCGCTGGCCACCAAATGATATTGCGAtt > 1:308147/1‑251 (MQ=255) ccGCAAAACCGAGGAGGAACATCAACGCTAGCGCGAGTCGGT‑CGCGAAAAACACGCCGGTGCTACATCCGGTGGTTCGCACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTAATACGCGAATTGTGGATGTGAGGGCGAAAGAAAGCGTAGCCTTGTTAAGTTTTTTGTTGGCCCATA‑CAGCCACAGCGTAGTTTCAGGGGCgctggcgcgggccacgacactgtagtgcgattt > 2:820482/1‑230 (MQ=255) aacccgggacgaaaCACCAACGCGGGCGCGGTGGGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGAt < 2:694258/239‑1 (MQ=255) aaaCCGGGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTc < 1:482863/207‑1 (MQ=255) aaaCCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGACGTTGCTACATCCGGTGGTGCGAACGCATCCGGAGAGCGGTAAACAGGCGCTGTTTGTGAATGAATGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGGTACGTTTTTTGTTTGCCCATATCA‑CCAAACCGGGGATTCAGGTGCGCTGGCGCTGGCAACCAAACGAGATTGCGAGTTGGGAt > 2:749940/1‑251 (MQ=255) aggaggAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCaa > 1:378558/1‑225 (MQ=255) aaCATCCACGCTGGCGCGAAGAGGT‑CGCTCAAAACCCGCAGTTGCTACATCAGGCGGTGCGAACGCATCCGGTGAGCGGACAACCGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGGAAGAGAGCGAAGCCTTGTTACGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGATTCAGGCGCGGTAGCGGTGACACCCTAATGATAATGCggcatgtggacaacgcgagaac > 2:555525/1‑230 (MQ=255) accAACGCTGGCGCGAG‑CGGTTCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAg < 1:96205/249‑1 (MQ=255) tCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑ACAAACCGGAGTTTCAGGTGGGCTGGCGCTGGCCACCAAATGATATTGAGATTTGGGGTAACCGGGTGAcccggc > 2:448422/1‑248 (MQ=255) aactcgggCGCGAGGCGGC‑CCAGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTGTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCAc < 1:150787/245‑1 (MQ=255) gggCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGcc < 1:384071/250‑1 (MQ=255) gggCGGT‑CGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGcc < 1:771409/250‑1 (MQ=255) ggCGGT‑CGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCg > 1:709311/1‑251 (MQ=255) ggCGGT‑CGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCg < 2:180481/251‑1 (MQ=255) gcaaaaacCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCt < 1:26758/248‑1 (MQ=255) gAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGc > 1:222933/1‑251 (MQ=255) aaaaacCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGGTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGc < 2:266449/250‑1 (MQ=255) | GCATACCGATGTGACATTTATTGAAACGCCACCCGCAGGGGCGATTCTGGCAGCTAAAGAGTTACCTTCGACCGGCGGTGATACGCTCTGGACCAGCGGTA‑TTGCGGCCTATGAGGCGCTCTCTGTTCCC‑TTCCGCCAGCTGC‑TGAGTGGGC‑TGCGTGCGGAGCATGATTTCCGTAAATCGTTCCCGGAATA‑CAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGT‑CGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATGAAGGCTTTACTACGCGAATTGTTGATGTGAGCGAGAAAGAGAGCGAAGCCTTGTTAAGTTTTTTGTTTGCCCATATCA‑CCAAACCGGAGTTTCAGGTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGC > NC_000913/388088‑388581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |