Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,787,610	IS5 (+) +4 bp	intergenic (‑1773/‑1372)	ileY ← / → glaH	tRNA‑Ile/glutarate dioxygenase GlaH

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	34 (0.420)	30/488	1.6	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2787610 =	0 (0.000)					intergenic (‑1773/‑1375)	ileY/glaH	tRNA‑Ile/glutarate dioxygenase GlaH
*	?	NC_000913	= 2290113	NA (NA)	55 (0.680)	48/490	0.6	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 2787613	0 (0.000)					intergenic (‑1776/‑1372)	ileY/glaH	tRNA‑Ile/glutarate dioxygenase GlaH

TTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/2288679‑2289162                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ttGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGGTATCTTTTT‑CACGGA‑CAAAACCA‑TAAAC‑TGTCTCACCATACTCA‑ACC‑ATTGATG‑CCAATGCTATGGGGGACTATaaggaagaggcg                                                                                                                                                                                                                                              >  1:504051/1‑245 (MQ=255)   taTAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCAGAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACGCCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGaa                                                                                                                                                                                                                                            <  1:390998/249‑1 (MQ=255)          gTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAAACCGCTGGATATCTTTTT‑CCAGGA‑CAACACCC‑TCAAC‑TGTCTCACCATAATCA‑AAC‑GTTGATG‑CCCAAGCTATGGGGGCATATAACGAAGGGGCGGA‑TAAGAggg                                                                                                                                                                                                                                   >  1:484174/1‑251 (MQ=255)          gTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCACCTGGATATCTTTTT‑CCAGGAACAA‑ACCA‑TCAAC‑TGTCTCACCATACCCA‑ACA‑GTTTTTG‑CCAATGCTATGGGGGAATATACAGAAGGGGCGGA‑TAAGAggg                                                                                                                                                                                                                                   >  2:859756/1‑251 (MQ=255)            cATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAAGAAGGTTCGAA‑TAAGCgggg                                                                                                                                                                                                                                  >  1:664481/1‑250 (MQ=255)               ttAATACTGAGTACTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAAACAGCTTGAAATCTTTTT‑TCAGGA‑CAAAACCA‑TCAAC‑TGTCTCACCCTACTCA‑ACA‑GTTGATG‑CACATGCTATGGGTGGATATAAGGAAGGTGCGCA‑TAAGCGGGGAAAt                                                                                                                                                                                                                              >  1:683131/1‑251 (MQ=255)                tAATACTGAGTTTTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAAt                                                                                                                                                                                                                              <  2:139324/250‑1 (MQ=255)                 aaTACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTTCCATCAACCCGCTGGCTTTCTTTTT‑TCCGGG‑CACAACCC‑TCAAC‑TGTCTCACCATAATCA‑ACA‑GTTTATG‑GCCATGCTTTTGGTGGATATTAGGAAGGATCGAA‑TACACGGGGGAAttt                                                                                                                                                                                                                            >  2:566150/1‑250 (MQ=255)                 aaTACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAAttc                                                                                                                                                                                                                            <  1:594216/251‑1 (MQ=255)                   tACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGGGAATATAAGGAAGGGGCGAA‑TAAGCGGGGAAAttctt                                                                                                                                                                                                                          >  1:275839/1‑251 (MQ=255)                       ggtgaTTCGGCGGCGCCATTCATTATAATGTATTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATTCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATtcttct                                                                                                                                                                                                                        <  2:650522/245‑1 (MQ=255)                       gAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCT‑ACA‑GTTGATG‑CCAATGCTATGTGGGAATATTAGGAAGGGGCGAA‑TAAACGGGGGAAtttttttcg                                                                                                                                                                                                                      >  1:550396/1‑244 (MQ=255)                         gTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGAGG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGc                                                                                                                                                                                                                    <  1:388864/251‑1 (MQ=255)                          gtacattgaaatgaattcattTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATTAAACAGCTGGATATCTTTTT‑CCCGGA‑AAACACCA‑TCAAC‑TGTCTCACCCACCTCA‑ACA‑GTTGATG‑TCACAGATATAGGGGAATATAAGGAAGGTGCGAA‑TAAGCGGGGGAATTCTTCTCGGCt                                                                                                                                                                                                                   >  2:292232/15‑251 (MQ=255)                                    cgcCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCACCTGGGTATCTTTTT‑TCAGGA‑CAACACCC‑TCCAC‑TTTCTCACCATACTCC‑ACATGTT‑ATT‑GCAATGCTATGGGGGAATATAAGGAAGGTGCGAAATAAG‑GGGGAAATTTTTTTCGGGTGaacaagct                                                                                                                                                                                                          >  2:175137/1‑243 (MQ=255)                                    cgcCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAAACAGCTTGATATCTTTTT‑TCAGGA‑CAAC‑CCACTCACA‑TTTCTCACCATACAAA‑ACA‑TTTTTTG‑CCAATGCTTTTGGTGGATATAAGGAAGGTGAGCA‑TAAGCGGGGAAATTCTTTTCGGCTGACTCAGtcc                                                                                                                                                                                                         >  2:636351/1‑250 (MQ=255)                                     gcCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATTAACCCCCTGGATATCTTTTT‑TCCGGA‑CAACACCA‑ACAAC‑TGTCTCCCCAAACTCACACG‑GTT‑ATG‑TCAAATCTTTGGGTGGATATAAGGGAGGGGCGGA‑TAAGCGGGGAAAtttttttttgccggcccaagcat                                                                                                                                                                                                        >  2:365957/1‑230 (MQ=255)                                         ttcattTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGGTATCTTTTT‑CCAGGA‑CAAAACCA‑TCAAC‑TGTCTCACCATAATCC‑ACA‑GTTGATG‑CCAATGCTATGGGTGGATATAAGGAAGGTGCGCA‑TAAGCGGGGAAATTCTTTTCGGCTTGCTCAGgcatttct                                                                                                                                                                                                    >  1:460454/1‑250 (MQ=255)                                                cAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGTGAAATTCTTCTCGGCTGACTCAGTCATTTCATttcttc                                                                                                                                                                                             <  1:702654/251‑1 (MQ=255)                                                                tCCGTTAATTTCAGAATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCAGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCACATGGATAACTTTTT‑CCATGA‑AAACAACA‑TCAAC‑TATCTCACCCTTCTCA‑AAA‑GTTGATT‑ACCATGATATGGGAGAATATCAAGAAGGAGCGAG‑AAAGCGGGGAAATTCTTTTCGGCTGACTCTCGCATTTCATTTCTTCTTTTTTGTGC‑CGaatt                                                                                                                                                                            >  2:816989/1‑248 (MQ=255)                                                                      aaTTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCCGCTGGATATCTTTTT‑TCAGGA‑CAACACCA‑TCAACCTG‑CTCACCATACTCA‑ACA‑GTTTATG‑CCAATGCTATGGGGGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTTTCGGCTGAATCCATCATTTCATTTATTCTTGGTTTAGC‑CGTttttttttc                                                                                                                                                                      >  2:39929/1‑248 (MQ=255)                                                                           cAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTa                                                                                                                                                                 <  1:859756/251‑1 (MQ=255)                                                                            aGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑Ta                                                                                                                                                                                                                                         <  1:420781/179‑1 (MQ=255)                                                                                     acttacACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTg                                                                                                                                                        <  1:584894/250‑1 (MQ=255)                                                                                       ttacACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTTCCATAAACCAGCTGGGTATCTTTTT‑TCCGGA‑CCACACCC‑TCAAC‑TTTCTCACCATACTCA‑ACA‑GTTTATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGAGAA‑TTAGCGGGGAAATTCTTCTCGGCTGGCTCCGTCATTTCATTTCTTCATGTTTTGGC‑CGATTTTTTTTCCCGTAAATGCCTTTAAt                                                                                                                                                     >  2:111278/1‑251 (MQ=255)                                                                                               atttCTTGTATATGCTGTTTTCCGTGTGGATTGTTTTTCGAATTATTGTCGGTCCCCAACATCAGCTGGTTATCTTTTC‑CCAGTA‑CAATTCCA‑CCAAC‑GGTCTCATCATATTTA‑ACA‑GGTGTTG‑CTAATGCTATGGGTGATTATAAGGAAGGTGCGAT‑TAATCTGTTAAATTCTTCCCTTCTTTCTACGTCATTTTATTTCTTCATGTTTGAGC‑TGATTGTTTCTCCCGTAAATGCCTTGAATCAGCCTAt                                                                                                                                             <  2:662984/249‑1 (MQ=255)                                                                                                aTTCTTTTATATGCTTTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACGCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                                                                                                                                            <  2:193070/251‑1 (MQ=255)                                                                                                       tataTGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                                                                                                                                      <  1:14776/250‑1 (MQ=255)                                                                                                         tatGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGATCCATAAACCAGCTGGGTATCTTTTTTCCGGGA‑CA‑CACCA‑TCACA‑TATCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGGGCGGA‑TAAACGGGGGAATTCTTCTCGGCTGGCCAAGGCAGTTTATTTTTTTATGTTTGTGCACG‑TTTTTTTTCCCGTAAAAGCCtttaaatcacctatttttacagtt                                                                                                                                   >  2:411193/1‑229 (MQ=255)                                                                                                          atGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGtt                                                                                                                                   <  1:111278/250‑1 (MQ=255)                                                                                                             cTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAAAACCA‑TCAAC‑TGTCTCACCATAATCA‑ACC‑GTTGGTG‑CCCATGCTATGGGTGAATATAAAGAAGGGGGGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTTATGTTTGAGC‑CGGTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTTGCCCGTttctt                                                                                                                               >  1:696484/1‑251 (MQ=25)                                                                                                                    ccGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc                                                                                                                           <  1:316027/248‑1 (MQ=255)                                                                                                                        cAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAAACCCCTTGCTATCTTTTT‑CCAGGA‑AAACACCC‑TCAAC‑TGTCTCACCATACTCA‑ACA‑ATTTATGGCCAA‑GCTATGGTTGAATTTAAGAAAGGTGCGAA‑TATGCGGGGAAATTCTTTTCGGCTGCCTCTGTCATTTCATTTTTTCATTTTTGTAC‑CCCTTTTTTTTCCCGTAACAACCTTTAATCAACCTATTTAGAccagtttttcccccttttat                                                                                                                    >  2:650239/1‑233 (MQ=255)                                                                                                                        cAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑TCCGGA‑CCACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGGGGATATAAAGAAGGTGCGAA‑TAAGCGGGGGAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGGAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTa                                                                                                                      >  1:874200/1‑249 (MQ=25)                                                                                                                              tgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttc                                                                                                                                                                                                     <  1:522249/165‑1 (MQ=255)                                                                                                                                             tGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCCGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                                               >  1:200985/1‑251 (MQ=255)                                                                                                                                                 ggTCCCATAAACCAGCTGGATATCTTTTT‑CAAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGAGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCCTGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                              >  1:769505/1‑248 (MQ=255)                                                                                                                                                       aTAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtctctc                                                                                     >  1:13086/1‑251 (MQ=255)                                                                                                                                                                 tGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGt                                                                           >  1:204160/1‑251 (MQ=255)                                                                                                                                                                  ggATATCCTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAATGAAGGTGCGAA‑TAAGCGGTGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTATATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGt                                                                           <  2:504051/250‑1 (MQ=255)                                                                                                                                                                    atatCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATc                                                                        <  1:39929/251‑1 (MQ=255)                                                                                                                                                                                 cggA‑CAACACAA‑TCAAC‑TGTCTCTCCATACGCA‑ACA‑GTTGAAG‑CCAATGCTAAGGGGGAATATAAGGAAGGTGCGTA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGACATTTCATTTCTTCCTGTTTGAGC‑CCATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCAATTTAGGGCGTTCTCCCCAGTTTTTAGCGAGATCTCTCCCACTGCCATATCCTTTGGTCCGcc                                                            >  2:1055/2‑251 (MQ=14)                                                                                                                                                                                   gA‑CAACCCCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGTTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCg                                                          <  1:66542/251‑1 (MQ=255)                                                                                                                                                                                       aacaCCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGaaa                                                       <  1:184405/251‑1 (MQ=255)                                                                                                                                                                                                 aaC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGCTCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGc                                              >  2:824624/1‑251 (MQ=255)                                                                                                                                                                                                 aaC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGc                                              >  1:250682/1‑251 (MQ=255)                                                                                                                                                                                                      gTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGc                                          >  1:312484/1‑251 (MQ=255)                                                                                                                                                                                                        ctcACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGt                                        >  1:180708/1‑251 (MQ=255)                                                                                                                                                                                                        ctcACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGt                                        >  1:763607/1‑251 (MQ=255)                                                                                                                                                                                                          cACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCCTTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCTACTGACGTATCATTTGGTCCGCCCGAAACAGGTAGGCCAGCGTg                                       >  1:536406/1‑250 (MQ=255)                                                                                                                                                                                                          cACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCg                                         <  1:226268/248‑1 (MQ=255)                                                                                                                                                                                                                    a‑aCA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGCAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGc                            >  1:88172/1‑251 (MQ=255)                                                                                                                                                                                                                            ggATG‑CCAATGCTATGGGTGAATATAATGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTAGCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGtt                       <  2:169922/249‑1 (MQ=255)                                                                                                                                                                                                                                     aTGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGt              <  1:363164/251‑1 (MQ=37)                                                                                                                                                                                                                                           tGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTc         >  1:528841/1‑250 (MQ=34)                                                                                                                                                                                                                                               tGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCa     >  1:409485/1‑250 (MQ=32)                                                                                                                                                                                                                                                gAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCaa    <  1:650239/250‑1 (MQ=32)                                                                                                                                                                                                                                                gAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAc   <  1:636351/251‑1 (MQ=31)                                                                                                                                                                                                                                                 aaTATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCCTTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAcc  >  1:785716/1‑251 (MQ=14)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TTGATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTT‑CCAGGA‑CAACACCA‑TCAAC‑TGTCTCACCATACTCA‑ACA‑GTTGATG‑CCAATGCTATGGGTGAATATAAGGAAGGTGCGAA‑TAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC‑CGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/2288679‑2289162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.