Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,088,0100CT88.2% 39.6 / ‑0.4 17intergenic (+150/‑274)metK/galPmethionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (3/12);  total (4/13)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01
*NC_0009133,088,0110CT88.2% 41.4 / ‑0.0 17intergenic (+151/‑273)metK/galPmethionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (3/12);  total (4/13)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

TTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATT  >  NC_000913/3087787‑3088251
                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                                
ttGGTCGTAAACAGTCCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACaaa                                                                                                                                                                                                                         <  1:37592/250‑1 (MQ=255)
         aaaaTTTCCGGGGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTATTCTTTCTACACCTGCGTTCAAAGGCCAGCTTCGCGCTGGCCTTTTTCTTTGGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAat                                                                                                                                                                                                               <  1:255625/248‑1 (MQ=255)
                    ggggAAAAAACCGACAAAGCGCAGCTGCTGCTCGTTGCTGCCGGTCTGAAGTAATCTTTCTTCCCCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCTTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCATCCACAACAGACAAAGATTTGTAATATTTTCAtatt                                                                                                                                                                                                    <  1:374324/250‑1 (MQ=255)
                                      gcgcAGTTGCGGCGCGATCCTGCCGGTCTGAAGTAATCTTTCTTCCCCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCTTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAg                                                                                                                                                                                  <  1:779/251‑1 (MQ=255)
                                             tgctgcGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAATGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTAc                                                                                                                                                                           >  2:304339/1‑251 (MQ=255)
                                                              gttcgaaaGTAATTTTTCTTCACCGTCGTCCAAAGCCCACCCCCGCACTGGCCTTTTTCTTTTGAATGGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCTCAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAg                                                                                                                                                          <  2:353940/245‑1 (MQ=255)
                                                                                                                gCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAattat                                                                                                        >  2:271484/1‑251 (MQ=255)
                                                                                                                 ccTTTTTCTTTTGAATAGTATTTCACGCCGCATCCGGCAAAAAAACCGCCCGTACAATAACATCATTCTTCCTGATCACGTTTCACCGCATATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAattatc                                                                                                       <  2:327779/251‑2 (MQ=255)
                                                                                                                       tCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCAt                                                                                                  <  1:263809/250‑1 (MQ=255)
                                                                                                                                                       aaaaaaaCCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAgtg                                                                 <  1:341808/251‑1 (MQ=255)
                                                                                                                                                                                             cACGTTTCACCGCAGATTATCAACACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCttt                            <  1:139259/250‑1 (MQ=255)
                                                                                                                                                                                                     aCCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCttt                                                                                                                                                                   <  1:28600/107‑1 (MQ=255)
                                                                                                                                                                                                     aCCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCttt                                                                                                                                                                   >  2:28600/1‑107 (MQ=255)
                                                                                                                                                                                                        gCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTACTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTACTCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCCTAACTAAGATAAATGTTAGTGTAAGCGATTACACTGTTGTGATTTGCTTCACATCTTTTACCGTCGTACt                >  2:161382/1‑251 (MQ=255)
                                                                                                                                                                                                                 catcaCAATTGAAATTGATTACACCAACCACAACAGACAAATATTTGTAATATTTTCATAATATTAGTCTGCTTTCACATTTGTTACAATTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCTATTACACTTATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATc        <  2:415484/250‑1 (MQ=255)
                                                                                                                                                                                                                     acaACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt   <  1:352339/251‑1 (MQ=255)
                                                                                                                                                                                                                      caACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAtt  <  1:271484/251‑1 (MQ=255)
                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                                
TTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATT  >  NC_000913/3087787‑3088251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑