Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,912,074	G→A	S532F (TCC→TTC)	glmS ←	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,912,074	0	G	A	100.0%	64.3 / NA	22	S532F (TCC→TTC)	glmS	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/7);  total (15/7)

CCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGT  >  NC_000913/3911862‑3912310                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             ccAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaac                                                                                                                                                                                                         >  1:95627/1‑250 (MQ=255)   aGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGcaacaac                                                                                                                                                                                                      >  1:353386/1‑251 (MQ=255)                     aCGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGc                                                                                                                                                                                    >  1:370621/1‑251 (MQ=255)                                              cATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCgtgt                                                                                                                                                           <  1:37397/251‑1 (MQ=255)                                                tGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGttt                                                                                                                                                         >  2:220543/1‑251 (MQ=255)                                                 ggTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTc                                                                                                                                                        >  1:207204/1‑251 (MQ=255)                                                   aaagcaagcAGCGGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACAAGCGGCATCTCGATGATGTGCATGTCATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAg                                                                                                                                                      <  2:13302/250‑1 (MQ=255)                                                      gCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTc                                                                                                                                                   >  1:284366/1‑251 (MQ=255)                                                           cagcTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCcag                                                                                                                                              <  1:90543/251‑1 (MQ=255)                                                            agcTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCTCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATAAGTTAGCGCCAGCGGCCCGTGTTTCAGTTCGAcagc                                                                                                                                             >  2:311319/1‑251 (MQ=255)                                                                  aGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTa                                                                                                                                       <  1:415114/251‑1 (MQ=255)                                                                        aCGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGAAGGCttt                                                                                                                                 >  2:390474/1‑250 (MQ=255)                                                                                                aCCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCttt                                                                                                         >  1:69563/1‑251 (MQ=255)                                                                                                                                             cTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACg                                                            >  1:159038/1‑251 (MQ=255)                                                                                                                                                  aaaaCCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCa                                                       <  1:85588/251‑1 (MQ=255)                                                                                                                                                                 aTCGGCGAAGACATACAACTGACCGCCACGTGCGCGAACTTCTTCAATGTTGAATTTCAGTGTTTCCAGCAATGCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCATCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCATCGTGAATGTAAGAGATCGCTTTCAACTGCAAGGAGCCTTCCAGCGCGATTGGGTACGGATCGCCACGGCCCAGGATCAGCGCGTGAt                                        <  2:210966/251‑1 (MQ=255)                                                                                                                                                                   cGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGt                                      >  2:84190/1‑251 (MQ=255)                                                                                                                                                                            cATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACGACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCagag                             <  1:315946/251‑1 (MQ=255)                                                                                                                                                                              tACAACTGACCGCCACGCGCGCGAACTTCTTCACTGTTGAATTTAAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTCAGAGATCTCTTTCAACTTCAATGCGCCTTACAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGGGATGTATTTCAgaga                            >  2:336082/1‑250 (MQ=255)                                                                                                                                                                                        cGCCACGCGCGCGCACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGcc                 >  2:374615/1‑251 (MQ=255)                                                                                                                                                                                                    gAACTTCTTCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCCTATCGGCATCAATTAGCGCCAGCGGACCGTGTATCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCCGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGTGGTTTGTCAGAGAAATCTTCATCCAGCGCTTCaa       >  2:3843/1‑249 (MQ=255)                                                                                                                                                                                                       cttcttCAATGTTGAATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGt  >  1:9426/1‑251 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             CCAGGTTACGCGGCTGGTCAACGTCGGTGCCTTTGATCAGCGCGACATGGTAAGCCAGCAGCTGCAGCGGAACGGTGTAGAAGATCGGTGCAATCACCTCTTCCACATGCGGCATCTCGATGATGTGCATGTTATCGCTACTTACAAAACCCGCATCCTGATCGGCGAAGACATACAACTGACCGCCACGCGCGCGAACTTCTTCAATGTTGGATTTCAGTTTTTCCAGCAATTCGTTGTTCGGTGCAACAACAATAACCGGCATATCGGCATCAATTAGCGCCAGCGGACCGTGTTTCAGTTCGCCAGCAGCGTAGGCTTCAGCGTGAATGTAAGAGATCTCTTTCAACTTCAATGCGCCTTCCAGCGCGATTGGGTACTGATCGCCACGGCCCAGGAACAGCGCGTGATGTTTGTCAGAGAAATCTTCTGCCAGCGCTTCAATGCGT  >  NC_000913/3911862‑3912310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑