Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,823,1620CT98.0% 154.1 / ‑4.0 51H255Y (CAC→TAC) spoTbifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (29/21);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01

TAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTG  >  NC_000913/3822917‑3823402
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                
tAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAACGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTAGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTAAAAGAGCAGCGTTTTTACTCg                                                                                                                                                                                                                                             >  2:243513/1‑251 (MQ=255)
 aGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCTCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGa                                                                                                                                                                                                                                            <  2:369184/251‑1 (MQ=255)
  ggTATCCACCACATTAAAACCGAACTCGACGAGCTGGGTTTTGAGGCGCTGTATCCAAACCGTTATCGCGTACTCACAGAAGTGGCGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGACGAGCGATTGCAGGAAGCGGGAATACCGTGCCGCGACAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGAGATCAAAGAGCAGCGTTTTTACTCGAt                                                                                                                                                                                                                                           >  1:398087/1‑251 (MQ=255)
                                 agCTGGGTTTTTAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGAGGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTc                                                                                                                                                                                                            <  2:43485/251‑1 (MQ=255)
                                   cTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCa                                                                                                                                                                                                           >  1:24728/1‑250 (MQ=255)
                                            gAGGCGCTGTATCCCAACCGTTATCGCGTAATCACAGTAGTGGTGAAAGCCGAGCGCGGCAACCGTACAGAGATGATCCAGAAGATTCTTTCTGAAATCGCAGGGCGTTTGCAGGCAGCGGGACTACCGTGCCGCGTCAGTGGTCGCGGGAAGCATCTTTATTCGATTTACTGCAAAATGGAGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTCCGCTTTCCGCGTGATCgagttcggaagagg                                                                                                                                                                                                 >  2:211961/1‑238 (MQ=255)
                                            gAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGTTTCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCg                                                                                                                                                                                                              <  1:211961/238‑1 (MQ=255)
                                                  cTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGCCATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTg                                                                                                                                                                                           >  2:229249/1‑251 (MQ=255)
                                                             ccGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGc                                                                                                                                                                                >  1:179148/1‑251 (MQ=255)
                                                               gTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGGTTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGCTTTACTGCAAAATGGTGCTCAATGAGCAGCGTTTTTACTCGCTCATGGACATCTACGATTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTg                                                                                                                                                                              >  2:183176/1‑251 (MQ=255)
                                                                  aTCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGGGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGc                                                                                                                                                                           >  2:336382/1‑251 (MQ=255)
                                                                                 aagTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAATATTCTTTCTGAAATCGAAGGTCGGTTTCAGTAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTg                                                                                                                                                            <  2:348433/251‑1 (MQ=255)
                                                                                         aaaGCCGCGCGCGGAAACCGTAAAGAGGTGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCGTCTTTCTTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGAACAGCCTGTACACGcc                                                                                                                                                    >  2:377184/1‑251 (MQ=255)
                                                                                           aGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGGGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCcgc                                                                                                                                                  <  1:117911/251‑1 (MQ=255)
                                                                                                cgcgcgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCg                                                                                                                                             >  1:303196/1‑251 (MQ=255)
                                                                                                     ggCAACCGTAAAGAGATGATCCAGAAGATGCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCcg                                                                                                                                        <  1:183176/251‑1 (MQ=255)
                                                                                                                    atgatCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTTCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGGCAATGATTCTGACACCTGTTATCGCGTGCGGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTatat                                                                                                                         <  1:257148/251‑1 (MQ=255)
                                                                                                                                     ttctGAAAGCGAAGGGCGTTTGCATGAAGCTGGAAGACCGTTCCGCGTTAGTGGTCGCGGGAAGCGTCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCTTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCGGACTCCTGTAATCGCGTGCGGGGCCAGATGCACAGCCTTTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCTAAAAg                                                                                                           <  2:398087/248‑1 (MQ=255)
                                                                                                                                      tctGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGCTGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCgc                                                                                                        >  2:136172/1‑249 (MQ=255)
                                                                                                                                         gAAATCGAAGAGCGTTTGCAGGAAGCGGGAATGCCTGGCCGCGTCGGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAAGGGTGTCCAAAAAGCAGCGTTTTTACTCGATCAAGAACATCTACGTTTTCCGCGTGATCGTCAATGATTCTGACTCCGGGTATCGCGTGCTGGGCCAGATACACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACgg                                                                                                    <  2:215875/251‑1 (MQ=255)
                                                                                                                                               gAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATc                                                                                               >  1:105406/1‑250 (MQ=255)
                                                                                                                                                    gCGTTTGCAGGAAGCGTGAATACCGTGCCGCGTCTGTGGCCGCGAGCAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCATGTACAAGCCGCGTCCGCGCCGCGTGAAGGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCtt                                                                                         >  1:155196/1‑251 (MQ=255)
                                                                                                                                                      gTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTg                                                                                       >  1:224755/1‑251 (MQ=255)
                                                                                                                                                          gCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATTTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcac                                                                                    >  1:356863/1‑250 (MQ=255)
                                                                                                                                                                     gAATGCCGTGCCGCGGAAGTGGGCGCGGGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGATCAGCTTTTTTACTCGATCATTGACATCGACGCTGTCCGCGTGATCGTCAATGATTCGGACCCCGGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCGTTGCACACCTAGATGATc                                                                        <  2:148373/251‑1 (MQ=255)
                                                                                                                                                                                  cgTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGCTGATCGGCCCGCACGgtg                                                           >  2:51600/1‑251 (MQ=255)
                                                                                                                                                                                   gtaaggggTCGCGAGAAGCAACTTTATTCGAGTTTCGGCAAAAGGGTGCACAAAGAGCAGGGTTTTTACTCTATCATGGGCATATACGCTTGCCGCGTGACCTTCAATGATTCTGACACCTCTTACCGCGTGTTGGGCCAGATGCACAGCCTGTAAAAGCAGCGTCCGGGCCGCGTGAAAGACTATATCGCCCTTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGgtgt                                                          <  2:115548/245‑1 (MQ=255)
                                                                                                                                                                                     cAGTGGTCGCGGGAAGTAATTTTATGCGATTTACTGCAAAAGGGGGCTCAAAGAGCAGCGTTTCTACTCGATCATGGACATCTTCGATTTCCGCGTGATAGGAAATGATTCTGACACCTGTTATCGCGTGCGGGGCCAGATGCACAGCTTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATACCGCCATTCCAAAAGCGAACGTCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGtgtg                                                         <  2:167309/250‑1 (MQ=255)
                                                                                                                                                                                         ggTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGt                                                    >  2:401980/1‑251 (MQ=255)
                                                                                                                                                                                             gcgAGAAGCATCTTTATTCGATTTATCGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATAATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTTTGACCCCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCt                                                                                          <  2:317839/209‑1 (MQ=255)
                                                                                                                                                                                             gcgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCt                                                                                          >  1:317839/1‑209 (MQ=255)
                                                                                                                                                                                             gcgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTg                                                  >  1:193516/1‑249 (MQ=255)
                                                                                                                                                                                              cgAGAAGCATCTTTATTCGATTTAATTCACAATGGTGCTCCAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATACCAAAAGCGAACGGCTATCAGTCCTTGCACACCTCGATGATCGGCCCTCACGGTGTGCCGGTTGAgg                                               >  2:191772/1‑251 (MQ=255)
                                                                                                                                                                                                          ttATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCg                                     <  1:143879/249‑1 (MQ=255)
                                                                                                                                                                                                           tATTCGATTTCCTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACCCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTACCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGt                                                                                            <  2:227690/193‑1 (MQ=255)
                                                                                                                                                                                                           tATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGt                                                                                            >  1:227690/1‑193 (MQ=255)
                                                                                                                                                                                                              tCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCg                                <  1:29474/250‑1 (MQ=255)
                                                                                                                                                                                                                  tttACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGa                                                 <  1:300618/229‑1 (MQ=255)
                                                                                                                                                                                                                  tttACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGa                                                 >  2:300618/1‑229 (MQ=255)
                                                                                                                                                                                                                     aCTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATg                        >  2:21525/1‑251 (MQ=255)
                                                                                                                                                                                                                             aTGGTGCTCAAAGAGTGTTGTTTTTACTCTATAATGGACAGCTACGTTTCCCGATTGATCGTAAAGGATTCGTACTCAGGTTATCGCGTGCTGGGCGAGAGTCAAAGCCTGGACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCTCCATTCCAAAAGCGATCGGCTATCAGTCTTTGCACACCTCGATGATCAGCACGCTCCGTGTGCCGGTTGAGTTCCAGAACTGTACCGTACATATGGACAAGAt                <  2:61101/251‑1 (MQ=255)
                                                                                                                                                                                                                              tGGTGCTCAAAGAGCAGCGTTTTTAATCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGAGCCGCGTGAACGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGGCCAGATCCGTACCGAAGAGATGGACCAGATg               >  1:95161/1‑251 (MQ=255)
                                                                                                                                                                                                                                   cTCAACGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGAGATCGTCACTGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGCAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCCAATCCGTACCGAAGATATGGACCAGATGGCggg          >  2:407338/1‑250 (MQ=255)
                                                                                                                                                                                                                                      aaaGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                                        >  1:178837/1‑170 (MQ=255)
                                                                                                                                                                                                                                      aaaGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                                        <  2:178837/170‑1 (MQ=255)
                                                                                                                                                                                                                                      aaaGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAc                     >  1:93198/1‑237 (MQ=255)
                                                                                                                                                                                                                                      aaaGAGCAGCGTTTTTACTCGATCAGGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAc                     <  2:93198/237‑1 (MQ=255)
                                                                                                                                                                                                                                       aaGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATg      >  1:170574/1‑251 (MQ=255)
                                                                                                                                                                                                                                       aaGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATg      >  1:411372/1‑251 (MQ=255)
                                                                                                                                                                                                                                          agcagcGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTACCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgt   <  1:405322/251‑1 (MQ=255)
                                                                                                                                                                                                                                           gcagcGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGCGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATCTGGACCAGATGGCGGAGATGGgtg  >  1:32096/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                
TAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTG  >  NC_000913/3822917‑3823402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: