Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,176	T→G	E48A (GAG→GCG)	glyQ ←	glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,176	0	T	G	100.0%	189.8 / NA	67	E48A (GAG→GCG)	glyQ	glycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/32);  total (35/32)

CCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCA  >  NC_000913/3724930‑3725420                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ccccAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGTTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGcgcgcg                                                                                                                                                                                                                                                  >  1:171545/1‑251 (MQ=255)             aGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGGCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCAt                                                                                                                                                                                                                                      >  1:267467/1‑251 (MQ=255)                gTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTgg                                                                                                                                                                                                                                   <  2:88323/251‑1 (MQ=255)                  cGGGTTTTCCCAGTTATCTTCAACGAAACGGCTGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGCACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTAGGGCGACGAGAAGGCTGGACATAAGCAGACGCCATCGGTTCTGGCCCCAGCGCGCGCAGCCAGGTCATTgggg                                                                                                                                                                                                                                 >  2:385248/1‑250 (MQ=255)                            cAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTcc                                                                                                                                                                                                                       >  1:314701/1‑251 (MQ=255)                                  ccTTCCACGAAACGGAGGTCGGGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGTTACAGCTCCTGAATATTGTCCAGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTTTAAACGGTTGGGGTTTTCGCCTTAGCGACCATCGTCCGGGCGACGAGAAGGCTGAACATAAGCTGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGcc                                                                                                                                                                                                                 <  2:263504/250‑1 (MQ=255)                                         cGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTcc                                                                                                                                                                                                          >  1:315692/1‑251 (MQ=255)                                         cGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTGTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTcc                                                                                                                                                                                                          <  1:385012/251‑1 (MQ=255)                                           aaaCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCCGCCGCCATCGTTTCTGGCCCCAGCGCGCGCAGACAGGTCCTTGGGTGAGAGGTTCCCGCGCCGACTTCCAt                                                                                                                                                                                                        >  2:327348/1‑251 (MQ=255)                                            aaCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGCGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGACGACTTCCATg                                                                                                                                                                                                       >  1:207021/1‑251 (MQ=255)                                            aaCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATg                                                                                                                                                                                                       >  1:15765/1‑251 (MQ=255)                                             aCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGCAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCCGCGCGCGCAGACAGGTCATTGGGTGCGCGGTTTCCGCGCCGACTTCCATGt                                                                                                                                                                                                      >  1:405753/1‑251 (MQ=255)                                                     cGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATgg                                                                                                                                                                                               <  1:189798/250‑1 (MQ=255)                                                            aGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGCATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTgggg                                                                                                                                                                                                                                 >  2:292799/1‑208 (MQ=255)                                                                gggTCCATGCCCAGCTCTTTCAGAGAACCGCGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGCCAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATggg                                                                                                                                                                                   >  2:39760/1‑250 (MQ=255)                                                                   ccctggcccagtgctTTCAGAGAACCGTGGTACAGCTCCTGAACATTGTCCGTCGATGGCTTAATGCCCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGGAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTACCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCa                                                                                                                                                                                <  2:167525/238‑1 (MQ=255)                                                                        gCCCCGCTCCTTCAGAGAACCGCGGTACCGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGGTTTCGCCGTAGCGACCATCTGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGTCGTCCATGTCCAATGGTTGAACAATGTTGCAGcccc                                                                                                                                                                           <  2:218574/251‑2 (MQ=255)                                                                           cAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAgcgcgc                                                                                                                                                                                                                                                   >  1:202543/1‑176 (MQ=255)                                                                           cAGCTCTTTCAGAGAACCGAGGTACAGCGCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAgcgcgc                                                                                                                                                                                                                                                   <  2:202543/176‑1 (MQ=255)                                                                                  ttCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGAGAGAGGTTCCCGCGCCGCCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                                                                                                                  >  1:282579/1‑250 (MQ=255)                                                                                    cAGAGAACCGAGGTACTGCTCCTGAATATTTTCCGGCGATGGCTATATGACCACCTGGAACTTATAGTAGGGCTGTAAACGGTTGGGGGTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGTTGCAGCCCTGGCGAGCCCAGt                                                                                                                                                               <  2:314659/251‑1 (MQ=255)                                                                                    cAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTCAACGGATGGGGTTTTCGCCGAAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGCACCATGGTGCAGCCCTGGCGAGCCCAGt                                                                                                                                                               >  1:385876/1‑251 (MQ=255)                                                                                     agagAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGt                                                                                                                                                               >  2:32736/1‑250 (MQ=255)                                                                                           ccGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTgag                                                                                                                                                                                                                              >  1:89342/1‑181 (MQ=255)                                                                                           ccGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTgag                                                                                                                                                                                                                              <  2:89342/181‑1 (MQ=255)                                                                                           ccGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGACCAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTg                                                                                                                                                        >  1:89704/1‑251 (MQ=255)                                                                                                       tCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGa                                                                                                                                             <  1:376604/250‑1 (MQ=255)                                                                                                         cTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGCACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATc                                                                                                                                           >  1:114611/1‑250 (MQ=255)                                                                                                            aaTATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGCCGAGAAGGCTGAACATCAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGATGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGAAAGGTAAGGATCAAGc                                                                                                                                       >  2:320047/1‑251 (MQ=255)                                                                                                             atatTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGcc                                                                                                                                      <  1:239103/251‑1 (MQ=255)                                                                                                              tatTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGGCAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCAAGTACTCCTGTAAGGTCAGGATCAAGccc                                                                                                                                     >  2:235970/1‑251 (MQ=255)                                                                                                                  gttggtcGATGGCTTAATGACCACCGCGAACTGAAAGGAGTGCTGTAAACGGTTGGGGTTTGCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGTTGAACATAAGCAGCCGCCATCGGCTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGGTGAACAATGGTGCAGCCCGGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTgg                                                                                                                                  <  2:280491/244‑1 (MQ=255)                                                                                                                    ccGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGGTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGaa                                                                                                                                <  1:2857/250‑1 (MQ=255)                                                                                                                      ggCGATGGCTTAATGACCACCTGGAACTGATAGTCGTGCTGTAAACGGTTGGGGTTTTCGCCGTAACGACCATCGGTCGGGCAACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGGAGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACACTGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTACGGTCAGGATCAAGCCCTGGAAggg                                                                                                                             >  2:83026/1‑250 (MQ=255)                                                                                                                            ggCTTAATGACCACCTGGAACTGATAGAAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGACGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCACTGGTTGAACAATGGTGCAGCACTGGCGAGCCCAGTAATCCTGTCAGGTCAGGATCAAGACCTGGCAGGTCCTGGt                                                                                                                       >  1:104513/1‑251 (MQ=255)                                                                                                                                tAATGACCACCTGGAACTTATAGTAAGGCGGGAAACGGTAGGGGTTTGAGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACAGAAGCAGCCGCCATTGGTACTGGCCCCAGCGCGCGGAGACATGGCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTAAAATGGTGGAACAATGGTGCAGCCCGGGCGAGCCCAGGAATCCTGTAAGGTCAGGATCAAGAACTTGAAGGGCCTGGTAt                                                                                                                     <  2:288250/249‑1 (MQ=255)                                                                                                                                tAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATc                                                                                                                    >  1:304073/1‑250 (MQ=255)                                                                                                                                 aaTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTGGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGTCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCaa                                                                                                                  <  1:242578/251‑1 (MQ=255)                                                                                                                                 aaTGACCACCTGGAACTGATAGTAGGGCGGTAAACGGTTGGGGTGTTCGCAGTAGCGACCATCGGTCGGGGGACGAGAAGGCTGAACAGAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCaa                                                                                                                  <  2:315692/251‑1 (MQ=255)                                                                                                                                  aTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGAGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGAAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGAATCac                                                                                                                  >  2:400796/1‑249 (MQ=255)                                                                                                                                         ccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGc                                                                                                          <  1:204731/251‑1 (MQ=255)                                                                                                                                            ggatatGATAGTAGAGCTGTAAGTGGTTGGGGTTGTCGCCGTAGCGACCCTCGGTCTGGCGACGAGAAGGCTCAACGTAAGCCGCCGCCAACGGTTCTGGCCCCAGCGCGCGCAGACAGGGAATTGGGTGAGAGGTTCCCGCGCCGTCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAAGCCTGTTAGGTGAGGATCAAGTCCTGGAAGGTCCTGGTATCTATCTTTTgta                                                                                                         <  2:269899/244‑3 (MQ=255)                                                                                                                                              aaCTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCAtatt                                                                                                     <  1:327348/251‑1 (MQ=255)                                                                                                                                               aCTGCTAGTAGTTCTGTAAACGGTTGGCGCTTTCGCCGTAGCGACCATCGGTCGGGCGACGATAAGGCTGAACATAAGCAGCCGCCCTCGGTTCTGGCCCCAGCGCGCGCAGCCAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTATTCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCAtatta                                                                                                    <  1:50181/251‑1 (MQ=255)                                                                                                                                                 tGATAGTAGTGCTGTAACCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGACGGCTGAACATAAGCCGCCGCCATCGGATCTGGCCCCAGCGCGCGCCGACAGGTCCTTGGGTGAGAGGTTCCCGCGCCGACTTCCCTGTCCACTGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTATTCCTGTAAGGTCAGCATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATattatt                                                                                                  >  2:263212/1‑251 (MQ=255)                                                                                                                                                  gagtgtagtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTAttt                                                                                                 <  2:202535/247‑1 (MQ=255)                                                                                                                                                           gCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGTTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTgg                                                                                         <  1:263212/250‑1 (MQ=255)                                                                                                                                                              gTAAACGGTTGTGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATAc                                                                                     <  1:46012/251‑1 (MQ=255)                                                                                                                                                                       tGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGATCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGAtt                                                                            >  1:359187/1‑251 (MQ=255)                                                                                                                                                                          ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATACGCAGCCGACATCGGTTCTGGACCCAGCGCGCGCAGACAGGTCATTGAGTAAGAGGTTCCGGCGTCGCCTTCCATGTCAAATGGTTGAACAATGGTGCCGCCCTGGCGAGCCAAGTCATCCTGTCAGGACAGGATCCAGCCCTGGACGGTCCTGGTATCAAACTTTTGCATATTATTTCGCGCTGGATACGTGTGGATTTa                                                                          >  1:233286/1‑250 (MQ=255)                                                                                                                                                                              ttCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaagg                                                                      >  1:239405/1‑250 (MQ=255)                                                                                                                                                                                   cGTAGCGACCATCGGGCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCCTGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCAGGATACGTGTGGATTTAAAGGAAggg                                                                 >  2:288625/1‑250 (MQ=255)                                                                                                                                                                                    gTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGACCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGAt                                                               >  1:24723/1‑251 (MQ=255)                                                                                                                                                                                    gTAGCGACCAGCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGAt                                                               <  1:235970/251‑1 (MQ=255)                                                                                                                                                                                          aCCATCGGTCGGGCGACGAGGACGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGAGAGAGGTTCCCGCGCAGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGGGCCAAGTAATCCTGTACGGTCAGGATCAAGCCCTGGCAGGTCCTGGCATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAGAGGAAGGGATCAGtat                                                         >  2:64734/1‑251 (MQ=255)                                                                                                                                                                                             aTCGGACGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGGGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGTTATCAAACTTTTACATATTATTTCGTGCTGGATACGTGTGGCTTTAACGGAAGGGATCATtatccc                                                      >  2:150459/1‑248 (MQ=255)                                                                                                                                                                                                 gtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCt                                                  >  2:170391/1‑251 (MQ=255)                                                                                                                                                                                                 ggcggGCGACGAGAAGGCTGAACCTAAGCAGGCGCCATCGGTTCGTGCCCCAGCGCGCGCAGACAGGTCAGTGGGTGAGAGTTTCCCGCGCCGACGTCCATGTCCAATGGTTGAACAATGCTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGGATCAATCGTTTGCATTTTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATTCCCGCt                                                  <  2:94278/249‑1 (MQ=255)                                                                                                                                                                                                    gggtgacgaGAAGGCTGAACATAAGCAGCCGCCATCTGTTCTGGCCCCAGCGCGCGAAGCCAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGTATCAAGCCCTGGAAGGTCCTGGTAGCAAACTTTTGCATATTATTTCGTGCTTGATACGTGTGGATTTAAAGGAAGGGATCTGTATTCCCGCtgga                                               <  2:142024/247‑1 (MQ=255)                                                                                                                                                                                                                             cagcCGCCAGCGTGTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCTGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAATCggg                      <  2:314701/251‑1 (MQ=255)                                                                                                                                                                                                                              agcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAATCTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGa                     <  1:32736/251‑1 (MQ=255)                                                                                                                                                                                                                               gccgccATCGGTTCTGGGCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGaa                    <  1:88042/251‑1 (MQ=255)                                                                                                                                                                                                                               gccgccATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGa                     >  1:138906/1‑250 (MQ=255)                                                                                                                                                                                                                                       cGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAgg            <  1:156174/251‑1 (MQ=255)                                                                                                                                                                                                                                           tCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGACGATATACAGTACGAAACGGGAAAAAGCAgggatt        >  1:415691/1‑248 (MQ=255)                                                                                                                                                                                                                                            ctgtccccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAACGCAGGGCtt        >  2:342829/5‑250 (MQ=255)                                                                                                                                                                                                                                            cTGGCCCCAGCGCGCGCAGACAGGTCATTGGTTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCtt        >  1:318409/1‑250 (MQ=255)                                                                                                                                                                                                                                              ggCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTaa      <  1:266008/250‑1 (MQ=255)                                                                                                                                                                                                                                                ccccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGc   <  1:84641/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cccAGCGCGCGCAGACAGGTCATTGGGTGAGAGGGTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCa  <  1:275783/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCA  >  NC_000913/3724930‑3725420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑