Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	179.1 / NA	52	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/13);  total (39/13)

ACGCCGTTACCGACCAGGTTAGTCA‑GCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCAC  >  NC_000913/3682270‑3682748                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          ccgcctttcccGACCAGTTTAGTCA‑GCCCCCGAGCTTCTGACAAAAAGCGGCCAATCCCGAGAACCAGCCCCAGACCCGCTACCGGAAAATGGCCCACCGCAGAGAGCCC‑CGCCCCCAGCACGATAACGCCCCTCCCCTTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGt                                                                                                                                                                                                                                      <  1:37539/242‑1 (MQ=255)        aaCCGACCAGGTTA‑TCAAGCGCACGGGCTTCGGAAATAAGGGGGTGGATACGGAGAACCGGCCCCAGCCCCCCTACCGGAAAAGGGCCCCCCGAAGAGGGCGC‑CGCCCCCAGCACAAAAAAGCCCCTCCCCGTTCCCCCTGCCGCCCTTT‑AAGAAGAAAGCAGCAACACGATTAACAGCGTGATTGGGGGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGta                                                                                                                                                                                                                                <  1:175334/249‑1 (MQ=255)          ccGACCTGGTTAGTCA‑CCGCACGGGCTTCTGAAAAAAAGCGGCCGATACCGAGGATCAGCGCCAGCCCCCCTCCCGGAAAAGGCCCCCCCGCAGGGAGCGC‑CCCCGCACGCACAATAAACCCCCTCCCCGTTCCCCCTGCCGCCCCTT‑AAGAAGAAAGCAGCACCACGATTAACAGCGTGATTTGGTGGACGATACCCATCTGACTGTTAGTG‑CCTGGGGCGATAAACACC‑GCCGCTATTGTCAGGTatat                                                                                                                                                                                                                             <  1:296918/251‑1 (MQ=255)                  gTTAGTCA‑GCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGc                                                                                                                                                                                                                     >  1:125328/1‑251 (MQ=255)                                  aGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACg                                                                                                                                                                                                      >  1:197878/1‑251 (MQ=255)                                      tCTGACATAAAGCGGACGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGACTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATAACGATGTGCCATCAAGGTTAAACGAGt                                                                                                                                                                                                   >  2:280654/1‑250 (MQ=255)                                         gacaaaaggggggcGATCCCGAGGATCGGCGCCAGACCCGCGCCCGGCAAATGGCCCCCCCCAGGGA‑CGTTCCCCGCCAGATCGATAAACCCACT‑CCCGGTGCCCCTTCCCCCCCTTGTAGAAAAAAGCGGCACCATGTTGAACAGGGTGATTGGGGGTATGATGTCCTTCTGTCTTTTAGTGTCCTGGG‑CGATAATCACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTa                                                                                                                                                                                                  <  1:386351/235‑1 (MQ=255)                                            aTAAAGCGGTCGATACCTAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGAGTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTGAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGGTGTGCCATCAAGGTGAAATGAGTAGCCTGt                                                                                                                                                                                            >  1:143592/1‑251 (MQ=255)                                                  cGGTCGATACCGAGGATCAGCGCCAGACCTGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGGTGTGCCATCAAGGTTAAACGAGTAGCCTGTCggggt                                                                                                                                                                                      >  2:81075/1‑249 (MQ=255)                                                             gAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TCGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCCGGTATATTGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGGCCAGccccc                                                                                                                                                                           >  2:182234/1‑250 (MQ=255)                                                                gATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGAGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACAAC‑GACGCTATTGTCAGGGATATCGATGTGCCATAAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCccacc                                                                                                                                                                         >  2:268141/1‑250 (MQ=255)                                                                 acatccgcCAGACCCGCTCCCGCAAAATGGCCCACGGCAGAGGGCGC‑CGCCGCCAGCACGAAAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATGAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACGCC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCcaccac                                                                                                                                                                       <  1:182234/246‑1 (MQ=255)                                                                          aGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGAAGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCAGCTATTGGCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGGCGGGATGACCAGCCCCACCACCGAATTAcc                                                                                                                                                              >  2:77477/1‑250 (MQ=255)                                                                                gCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGcc                                                                                                                                                        >  1:79400/1‑251 (MQ=255)                                                                                        cAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGAGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGCGTAGCCTGTCGGGATTACCAGCCCCACCACCGATTTCAGGCAGCCGAGTTtct                                                                                                                                                >  2:287145/1‑251 (MQ=255)                                                                                                  ccctcagggagCGC‑CGCCGCCACCACGATAAAGCCCCTCCCCGTTCCCCCGCCCCCCCCTT‑TAAAAAAAAGCAACAACACGTTTACCAGCGTTATTTGGTGGACAATACCCATCTGTCTGTTAGTGGCCGGGG‑CGAAAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTc                                                                                                                                      <  1:248390/243‑1 (MQ=255)                                                                                                  aCCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTc                                                                                                                                      >  1:366762/1‑251 (MQ=255)                                                                                                        ggagcgcgcgccccccAGCACGATAACGCCACTCCCCGTGCCCCCTCCCCCCCCTT‑AAAAAAAAAGCACCACCACGATAAACGGGGTGATTTGGTGAACGTTTCCCATCTGATTGTTAGGGGCCGGGG‑CAATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCa                                                                                                                                 <  1:146061/237‑1 (MQ=255)                                                                                                                      ccagcacaaaaaagcccccccccGTTCCCCCGGCCGCCTCTT‑AAGAAGAAAGCAACAACACGATTACCAGCGTGATTTGGTGGACAATATCCATCTGATTGTTGGTGCCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGcc                                                                                                                   <  1:104594/231‑1 (MQ=255)                                                                                                                              aTAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAg                                                                                                                       >  1:109561/1‑239 (MQ=255)                                                                                                                                    ccACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTCGCCTGTCGGGATGACCAGCCCCACCACCGCTTTACGGCAACCGCGTTTCTCCATCTTTACGAGCCTACCCGGCAGCGCCGACTCCGAAGa                                                                                                        >  2:173335/1‑248 (MQ=255)                                                                                                                                          cccGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGCCGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAACCACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGGTGACCAGCCCCACCACCGATTTACGGCAGCCGCGTTTCTCCATCTTGTCGAGCATAAGCGGCAGCGCCGACTCGGAAGATGAAGTccc                                                                                               >  2:205457/1‑251 (MQ=255)                                                                                                                                             gttcccccTGACGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACCGCGTGATTTGGTGGACGCTATCCATCTGACTGTTAATGACCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGCTGTGCCAGAAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCCGCAGGATTTCTCCATCTTGTGGAGCCTACGCGGCCGGGCCGAATCGGAAGAGGAAGGcccccg                                                                                            >  2:248462/5‑249 (MQ=255)                                                                                                                                               tcccccTGCCGCCCTAT‑TAGAAAAAAGAACAAACAGGTTTAACAGTGTGTTTTGGGGGACGATATCCAACTCCCTGTTTGGGGCCGGGG‑GGATAACCACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGAGGAAGTCCCCAGt                                                                                           <  1:300825/248‑1 (MQ=255)                                                                                                                                               tACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGCTGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGt                                                                                           >  1:273703/1‑250 (MQ=255)                                                                                                                                                        cgccCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGCTATCCATCTGACTGTTCGAGGCCTGGG‑CGATAAACACC‑GCCGATATTGTCAGGTCTATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGCTTTACGGCAGCCGAGTGTCTCCTTTCTGTCGAGCATACGCGGCAGCTCCGCCCCGGGAGATGACCTCCCCCGTCCAATcagc                                                                                  >  2:122882/1‑250 (MQ=255)                                                                                                                                                        cgccCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATcagc                                                                                  >  1:256427/1‑250 (MQ=255)                                                                                                                                                         gccCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGAt                                                                                                                                                                   >  1:113536/1‑168 (MQ=255)                                                                                                                                                            ccTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGGAGCCTGTCGGGATGACCCGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttc                                                                             >  1:78124/1‑251 (MQ=255)                                                                                                                                                                      gaaAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCaagg                                                                      >  1:200367/1‑246 (MQ=255)                                                                                                                                                                       aaaGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATg                                                                   >  1:407505/1‑251 (MQ=255)                                                                                                                                                                       aaaGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCAACAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGAGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGATCTTCACGGATg                                                                   >  1:64088/1‑251 (MQ=255)                                                                                                                                                                           cagcaACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCa                                                                                                                                                                                 >  1:346109/1‑137 (MQ=255)                                                                                                                                                                                  acGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGAATCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATaa                                                         >  1:236531/1‑250 (MQ=255)                                                                                                                                                                                     aTTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAAttt                                                     >  1:260267/1‑251 (MQ=255)                                                                                                                                                                                     aTTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTAAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCCGGTATATCGATGTGCCCTCAAGGGTAAACGAGTCGCCTGTCGGGTTGACCAGCCCCACCACCGATTTACAGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTACCCAGGACACTCAGCCGTTCTTTACGGATGTAGCGGATAAAttt                                                     >  2:280321/1‑251 (MQ=255)                                                                                                                                                                                       tAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACCCC‑GCCGCTATTGTCAGGTCTATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTgg                                                   >  1:53592/1‑250 (MQ=255)                                                                                                                                                                                        aaCAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGCTGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGACGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGa                                                   >  1:163927/1‑250 (MQ=255)                                                                                                                                                                                        aaCAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGGGTTTCTCCATCTTGTCGAGCAt                                                                                                                                >  2:90842/1‑173 (MQ=255)                                                                                                                                                                                        aaCAGCGTGATTTGGTGGACGATACCCATCTGACTGTTAGTGGCCTGGG‑CGAAAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCAt                                                                                                                                <  1:90842/173‑1 (MQ=255)                                                                                                                                                                                                   tggggggacaaaatCCATCTGACTGTGAGTGGCCTGGG‑CGAAAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGaaa                                        <  1:81075/238‑1 (MQ=255)                                                                                                                                                                                                      gtggACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCCGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATAAGCAGTTCTTCACGGGTGTAGCGGATAAATTTAAAGATACTGAAACCac                                    >  2:217491/1‑250 (MQ=255)                                                                                                                                                                                                           cGATATCAATCTGACTGTTAGTGGCCTGGG‑CGATAAAAACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCAATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTAAAATCCGCAGTTCTTCACGGCTGGAGCGGATAAATTTGAAGATACTGAAACCAGGCGCt                               >  2:233133/1‑251 (MQ=255)                                                                                                                                                                                                            gATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAGCACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCCGCAGTTCTTCACGGATGTAGCGGATAAATTTTAAGATACTGAAACCAGTCGCtt                              >  2:251128/1‑251 (MQ=255)                                                                                                                                                                                                            gATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGACGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGCACAATCCGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGAAACTGAAACCAGTCGCtt                              >  2:161171/1‑251 (MQ=255)                                                                                                                                                                                                                ccagcttgttttgtggTGGCCGGGG‑AGAAAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACTCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGc                          <  2:256427/236‑1 (MQ=255)                                                                                                                                                                                                                   aTCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGAt                       >  1:81861/1‑251 (MQ=255)                                                                                                                                                                                                                        aCTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGACGTTCTTCACGGATGTAGCGGATAAATTTGAAGCAACTGACACCAGTCGCTTTAGCGATTGAAc                  >  2:360104/1‑251 (MQ=255)                                                                                                                                                                                                                              aGTGGCCTGGG‑CGATAAACACC‑GCCGCTATTGTCAGGTCTATCGATGTGCCATCCAGGGTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGCTTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACCCGGAAGATGAAGTCCCCAGTAAAATCCGCAGGTCTTCCCGGGTGTAGCGGATAAATTTGAAGATACTGCAACCCGCGGCTTTAGCGGTTGAACCCAAt             >  2:41539/1‑250 (MQ=255)                                                                                                                                                                                                                                tgtgcctcggagaaaaaCACCCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAAt             <  2:366762/236‑1 (MQ=255)                                                                                                                                                                                                                                      ggg‑CGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCa                                                                                                                        >  1:163126/1‑135 (MQ=255)                                                                                                                                                                                                                                          cGATAAACACC‑GCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGGACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGcaccac  >  2:45047/1‑250 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          ACGCCGTTACCGACCAGGTTAGTCA‑GCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGT‑CGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTT‑TAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGG‑CGATAAACACC‑GCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCAGCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCAC  >  NC_000913/3682270‑3682748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑