Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,384,808	C→T	K305K (AAG→AAA)	yfbK ←	IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,384,808	0	C	T	100.0%	81.0 / NA	29	K305K (AAG→AAA)	yfbK	IPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/10);  total (19/10)

CAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAACC  >  NC_000913/2384565‑2385050                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    cAATACTTGCTGCGCTTCAGAGAGGGGATCAATGTAGCTGGAGTTGCCGTTACCAATAGCGTCAATTCGCACCATCATAGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGGCGACAGAGTAACACCAGACTCCGGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGGGAAAGTCAACGTCAGTGGCTAATAAAAGGCGATTGATGCCGACCTTAATAATCCCTTtcttcg                                                                                                                                                                                                                                              <  2:369946/251‑1 (MQ=255) cAATACTGTCTGCCCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACATAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtcgtcg                                                                                                                                                                                                                                              <  2:207211/251‑1 (MQ=255)      cTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCctgc                                                                                                                                                                                                                                         >  1:369907/1‑251 (MQ=255)         tCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGTCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCctgctg                                                                                                                                                                                                                                       <  1:193069/250‑1 (MQ=255)                  cAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGt                                                                                                                                                                                                                             >  1:305360/1‑251 (MQ=255)                      gagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACAATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCACTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTcc                                                                                                                                                                                                                          >  1:243378/1‑250 (MQ=255)                             tCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCgg                                                                                                                                                                                                                   <  2:305360/250‑1 (MQ=255)                              cAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGc                                                                                                                                                                                                                  <  1:227883/250‑1 (MQ=255)                                    cgatatAGTTGCCGTTACCACCATCGGCAACTCGCACCATCATTGCATCGTTGTAATTGCTATTCCCCACGCCAAACGTCGAAAGAGTAACACCAGACTCCCGCTGTTATTTGACCATTGATGCAATCGATTTAGGATAGTCAATGCCAACGTTAAAGTCAACGTCAGTGGCTACTAAAATGCGATTGCTGCCGCACTTACTAAACCCTTTCGTCGCATGATGATAAGCCAGTTCCAGACCGTCACCGcc                                                                                                                                                                                                            >  2:11867/6‑250 (MQ=255)                                                    aCCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCgg                                                                                                                                                                                           >  1:313463/1‑251 (MQ=255)                                                            gggCAATTCGTACCAGCATTGCCTCGTTGTATTTGCTATCCCCCACGCCAAACGTCGACAGATGAACCCCAGATTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCAAACGTAAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATTCCGCCCTTAATAAACCCTTTCGGCGCCTTCTGATAAGCCAGTTCCAGCCCGGCACCGCCaggtgtactgactggggaatgaaga                                                                                                                                                                                   <  1:417680/250‑25 (MQ=255)                                                                                   tCGTTGTAATTGCTATTCCCCACGCCAAACGTCTACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtcgtc                                                                                                                                                                                                                                               <  1:344834/168‑1 (MQ=255)                                                                                   tCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTtcgtc                                                                                                                                                                                                                                               >  2:344834/1‑168 (MQ=255)                                                                                         aaaTTGCTATTCCCCCCCCCAAACGTCGACAGAGTAACCCCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGTATCGTAAAGGCCAACGATAAAGTCACCTTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAACCCAGTCCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCtt                                                                                                                                                      <  1:382203/250‑1 (MQ=255)                                                                                                    ccccACGCCAAACGTCGACAGAGTAACACCAGACTCCCGTTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCTTTTCGTCGCCTGCTGATAAGCCAGTGCCAGCCCGGCACCGCCATTGGTACTGCCTTCTGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCgg                                                                                                                                           <  1:194281/251‑1 (MQ=255)                                                                                                                   cGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaatg                                                                                                                            >  1:410491/1‑251 (MQ=255)                                                                                                                    gACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaatgc                                                                                                                           >  1:253968/1‑251 (MQ=255)                                                                                                                                                   tGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATg                                                                                            >  1:117077/1‑251 (MQ=255)                                                                                                                                                           gATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGtt                                                                                    >  1:140839/1‑251 (MQ=255)                                                                                                                                                            aTTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGtt                                                                                    >  1:343471/1‑250 (MQ=255)                                                                                                                                                                   cgtttttGGATCGACAATGCCAACGTTAAATCCACCGTCAGGGGTAAAAAAAATGCGATTGATGCCCCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCGGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCt                                                                            <  2:85645/248‑1 (MQ=255)                                                                                                                                                                                                       gTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGGTGGCAATGTTATCCTGATCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACt                                       >  1:32631/1‑251 (MQ=255)                                                                                                                                                                                                          aGTGGCTAAGAAAATGCGATTGCTGCCGCCCTTAATAAACCCTTTCGTCGACTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGTCTTGTGCCTCCCGGAGCTAGAAGGCAATGCAATACGTGAGCCGCCAGCGTAGGTCACTGTGGCACTGGTATCCTGCTCACGAAGTTCTGTTG‑CCAATAATTTCAACGAAGAccgg                                     >  2:131625/1‑247 (MQ=255)                                                                                                                                                                                                            tGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCCTTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCt                                                                               >  1:378080/1‑207 (MQ=255)                                                                                                                                                                                                              gCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACTGGATa                                  >  1:133711/1‑249 (MQ=255)                                                                                                                                                                                                                        tGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGt                      >  1:99142/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTg      >  1:330455/1‑250 (MQ=255)                                                                                                                                                                                                                                            ataaACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAAcc  >  2:6154/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaaCCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAAcc  >  1:369706/1‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCT‑TTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAACC  >  NC_000913/2384565‑2385050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑