Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,035	G→A	A246T (GCG→ACG)	metG →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,035	0	G	A	93.3%	78.4 / ‑5.1	31	A246T (GCG→ACG)	metG	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/7);  minor base C (0/2);  total (21/10)
Fisher's exact test for biased strand distribution p-value = 8.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCTAT  >  NC_000913/2194821‑2195277                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTAAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGAAAATAAAATGCAGGAGTGGTTAGCATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAAAACGCCGGACAAATCTTTTCACGTCTGGATGGACGCAc                                                                                                                                                                                                                 >  2:351736/1‑251 (MQ=255)  aaaTCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCAcc                                                                                                                                                                                                                >  2:297815/1‑251 (MQ=255)       gggggtTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACTCTTCTGCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGTTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATACCTTCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTg                                                                                                                                                                                                           <  1:247266/248‑1 (MQ=255)          ggtgtCTGGCGCTACGACGGTAATGCGTGATTCGGAACACGTCTTTGTTGATCTGCCCTTGTTCAGCGAAATGGTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAAGGCAGGAGTGGGTTGAATATGGCCGGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACTCGCCGGGCAAATATTTCTAAGTCTGGCTGGACGCACCGATTGGc                                                                                                                                                                                                         <  2:108488/246‑1 (MQ=255)                                     tGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCg                                                                                                                                                                            >  1:189873/1‑251 (MQ=255)                                           tGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCCGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCCAATATTTCTACGTCTGGCTGGACGCACCGATTGGCGACATGGGTT‑CTTTCAAGAATCTGTGAGACAAgc                                                                                                                                                                      >  2:322065/1‑251 (MQ=255)                                                  ttcttcttTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTTTGCGACAAGCgcggcg                                                                                                                                                                >  1:188604/1‑250 (MQ=255)                                                   tcttcttTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGGGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGAc                                                                                                                                                              >  2:89957/1‑251 (MQ=255)                                                        tttGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCACATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCTACAAGCGCGGCGACAGCGt                                                                                                                                                         >  2:204309/1‑251 (MQ=255)                                                        tttGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGt                                                                                                                                                         >  1:327443/1‑251 (MQ=255)                                                                         cAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTGGCAGGAGCAGGTGGCAAAGAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACt                                                                                                                                        <  1:187196/251‑1 (MQ=255)                                                                            cGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTgg                                                                                                                                      >  1:259020/1‑250 (MQ=255)                                                                              aaaTGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGTTGGACGCACCGATTGGCTACATTGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGga                                                                                                                                     >  1:182446/1‑249 (MQ=255)                                                                                               ccccGCAGCGGTGCGTGTCAGGGTCAGGGGTAAAATAAAATGAAGGAGTGGTTTGAATTGGGCCTGCAACAGTGGGATATCCCCCGCGCGGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGGCTGGTTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAAAATCTGTGCGACAAGCGCGGCTACAGCGTAATCTTAGATGAATACTGGAAGAAAGAATCCACCGCCGa                                                                                                                  <  2:35777/250‑1 (MQ=255)                                                                                                    cAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACAGCCGGGCTgg                                                                                                             >  2:336652/1‑250 (MQ=255)                                                                                                        ggTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTAcc                                                                                                          >  1:398630/1‑250 (MQ=255)                                                                                                                        aGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTaa                                                                                             >  1:63647/1‑247 (MQ=255)                                                                                                                          gTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATATCCCGCGACGCCACTTACTTCGGTTTTGAAATTCCGAACACGCCGGCCCACTATTTCTACGTCTGGTTGGACGCACCGATTGCCTACATGGGTT‑CTTTCAAGAATCTGTGCGACACGCGCGGGGACAGCGTAAGCTTTGATGAATACTTGAAGAAAGACTCCACCGCGGAGCTGTACCAATTCAtaggacaataaat                                                                                       >  2:96135/1‑239 (MQ=255)                                                                                                                                      aTGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTcc                                                                            >  1:371938/1‑250 (MQ=255)                                                                                                                                         cAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAg                                                                        <  1:89957/251‑1 (MQ=255)                                                                                                                                                                gCAACAGTGGGATATCCCCCGCGACGCCCCTTATTTCGGTTTTAAAATTCCGACCACGCCGGGCAAATATTTCTCCGTCGGGTTGGACGCACCGATTGGCTAAATTGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACGGCGTAAGCTTCGATGAATACTGGAAGAAAGACCCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCt                                                   <  2:182446/249‑1 (MQ=255)                                                                                                                                                                          aatatCCCCCGCGACCCCCCTTTCTTCGGTTTTGAAATACCGAACACGCCGGCCAAATATTTCTACTTCTGGCTGGCCGCACCGTTTGGCTACATGGTTT‑CTTCCAAGAACCTGTGCGACAAGCGCGGCGACAGCGTAAGTTTCGATGAATACTGGAAGAAAGATCCCACCGCCGAGCTGTACCACTTCATAGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCaa                                       <  1:199198/250‑1 (MQ=255)                                                                                                                                                                                tcCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCg                                 >  1:305065/1‑251 (MQ=255)                                                                                                                                                                                      agcGCCCCTTACGTCGGTGTGTAAAGTCCGAACACGCCGGGCAAATATTTCTACTCCTGGCTGAACGCCCCGATTGTCTACAGGGTTT‑CTTTCAAGAATCTGTGCGAAAAGCTCGGCGACAGCGTAAGCTTCGATGAAATCTGGAAGAAAGACTCCCCCGCCGATCTTTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGcc                           <  1:351736/249‑1 (MQ=255)                                                                                                                                                                                          ccccTTACTTCGGTTTTGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTcc                       >  1:347373/1‑251 (MQ=255)                                                                                                                                                                                           cccTTACTTAGGTTTGGAAATTCAGCACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACAACTTCCTCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTAGCCTGCCCTGCTGGAAGGCAGCAACTTCCGCACGCCGTcc                       >  2:378012/1‑250 (MQ=255)                                                                                                                                                                                                   tCGGTTTTGACATTCCGACCACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGCTGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGCAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCAGTCCCACAtgttt              >  2:294565/1‑251 (MQ=255)                                                                                                                                                                                                   tCGGTTTTGAAATCCCTACCCCGCCGGGCAAATATTCCTAAGTCTGGCTGGCCGCACCGATTGGTTAC‑TGGGTTTCTTCCAAGAATCTGTGCCACAAGGGCGGCGACATCGTAAGCTTAGATGAATATTGGAAGAAAGACTCCCCCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCtgt                <  1:313376/249‑1 (MQ=255)                                                                                                                                                                                                      gtttgaaaatgcggAACCCGCCGGGAAAATTTTTGTCCGTGTGGGTGGCCGCACCGATTGGCTAAATGGTGT‑CTTTAAAGAATCTGTGCGAAAAGCGGGGCGACAGCGTAAGCTGCGATGAATACTGGAAAAAAGACGCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCtgtttgtt           <  2:189873/238‑1 (MQ=255)                                                                                                                                                                                                        tttGAAATTCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTcc         >  2:398252/1‑250 (MQ=255)                                                                                                                                                                                                               ttCCGAACACGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCTAt  >  1:59252/1‑251 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GAAATCGGTGGTTTCTGGCGCTACGCCGGTAATGCGTGATTCTGAACACTTCTTCTTTGATCTGCCCTCTTTCAGCGAAATGTTGCAGGCATGGACCCGCAGCGGTGCGTTGCAGGAGCAGGTGGCAAATAAAATGCAGGAGTGGTTTGAATCTGGCCTGCAACAGTGGGATATCTCCCGCGACGCCCCTTACTTCGGTTTTGAAATTCCGAACGCGCCGGGCAAATATTTCTACGTCTGGCTGGACGCACCGATTGGCTACATGGGTT‑CTTTCAAGAATCTGTGCGACAAGCGCGGCGACAGCGTAAGCTTCGATGAATACTGGAAGAAAGACTCCACCGCCGAGCTGTACCACTTCATCGGTAAAGATATTGTTTACTTCCACAGCCTGTTCTGGCCTGCCATGCTGGAAGGCAGCAACTTCCGCAAGCCGTCCAACCTGTTTGTTCATGGCTAT  >  NC_000913/2194821‑2195277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑