Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	109.8 / NA	33	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/15);  total (18/15)

GGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTCGCC  >  NC_000913/1894599‑1895084                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ggTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAg                                                                                                                                                                                                                                             <  1:197327/251‑1 (MQ=255)     ggccagcacccctgctGCCCCCAGCCCGATTGCCCTGCCGCTGCTCATCCCTTGTTCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTAGTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGTCTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGGCAgggc                                                                                                                                                                                                                                          <  2:167884/236‑3 (MQ=255)              ctctTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGCGTGAAGGTAAACCTGCACACATTGTTAACTCCTGCTAAATTGTTGGCGCTCATTATTTCATGCTACCCGGCACATAACCAGTAGAGTCAGGACTGATGAAGACGTTATATCCCGCTGTCATTACTTTACCCTGGCGTCAGGACGCCGCTGAAt                                                                                                                                                                                                                                >  2:339989/1‑251 (MQ=255)                        accaGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTACTTATTTCATGCTACCCGGCACATAGCCAGAAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGAGATTACTTTACCCTGGCGTCCGGGCGCCGCTGAATTTTATTtctc                                                                                                                                                                                                                      >  2:28180/1‑251 (MQ=255)                             cgcgATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCt                                                                                                                                                                                                                 >  1:310483/1‑251 (MQ=255)                               cgATTGCCTGGCCGCTGCTCATCCCTTTTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCTCTTa                                                                                                                                                                                                               <  2:411725/251‑1 (MQ=255)                                   tGCCTGGCCGCTGCTCATCCCTTGAGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGCATTTTATTTCTCCCGCTTAAGcc                                                                                                                                                                                                           >  1:48539/1‑251 (MQ=255)                                        ggCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCt                                                                                                                                                                                                       <  1:301269/250‑1 (MQ=255)                                           cGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCg                                                                                                                                                                                                   <  2:82704/251‑1 (MQ=255)                                                   aTCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCAAATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACCCCGCTGACTTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGAt                                                                                                                                                                                           >  2:105894/1‑251 (MQ=255)                                                                         ggATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGGTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGc                                                                                                                                                                      <  1:270968/250‑1 (MQ=255)                                                                           aTCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCg                                                                                                                                                                    >  2:404645/1‑250 (MQ=255)                                                                                ccctgtaGCTTTTTTCTGCTGTTCATGTGTGAGTGAAGGTAAACCTGCAACCATTGTTAACTCCTGCTAAATTGTTGGCTCTTATTATTTCATGCTTCCCGCCACATAGCCAGTAGAGTCAGGACTGATGAATACGTTATTTCCCGCTGTTATTACTTTTCCCTGTCGTCAGGACTCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACGCCGGCTATGCCGatcatc                                                                                                                                                              <  1:16094/245‑1 (MQ=255)                                                                                         tttttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGAcgcc                                                                                                                                                                                                                                       >  1:194643/1‑169 (MQ=255)                                                                                         tttttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGAcgcc                                                                                                                                                                                                                                       <  2:194643/169‑1 (MQ=255)                                                                                                                           ccTGCAAACATTGTTAACGCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTCCCCGGCACATAGCTAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTTTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTGTGATATTGTGGGCGCCGAGCCGATTTGCAc                                                                                                                   <  1:165830/251‑1 (MQ=255)                                                                                                                                        ttAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTc                                                                                                      >  1:17756/1‑251 (MQ=255)                                                                                                                                           aCTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGt                                                                                                   <  1:405716/251‑1 (MQ=255)                                                                                                                                                      aTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGc                                                                                                                                                                                              <  1:12395/149‑1 (MQ=255)                                                                                                                                                      aTTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGc                                                                                                                                                                                              >  2:12395/1‑149 (MQ=255)                                                                                                                                                           tgGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCgttgtt                                                                                    >  1:123769/1‑250 (MQ=255)                                                                                                                                                                           aTGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACg                                                                   >  1:395532/1‑251 (MQ=255)                                                                                                                                                                                     gCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGAcc                                                         <  1:119217/251‑1 (MQ=255)                                                                                                                                                                                       acaTAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGAtt                                                                                                                        >  1:319808/1‑186 (MQ=255)                                                                                                                                                                                             ccAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGCCGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGCTTTGCACTTTAACCACTTTCGGTACAGAAACCGTTGTTAGTGACAGCGAAAAACGCACAACGCCCACtgatg                                                  >  2:318104/1‑250 (MQ=255)                                                                                                                                                                                                         ggACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTAAACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGTTCTTGTGGTCGCCACGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTATTGAAAGCAAAAAACGCACAACGACCACTGATGACCCGCTACAGGt                                     >  2:35317/1‑251 (MQ=255)                                                                                                                                                                                                                    gACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTCTTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCt                                                                                                                                                                          >  1:409848/1‑107 (MQ=255)                                                                                                                                                                                                                    gACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAATGAAACCGTTGTTAGAGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCGGCAgg                          >  2:66899/1‑251 (MQ=255)                                                                                                                                                                                                                    gACGTTAGCTCCCGCTGTGATTACTTTACCCGGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCCCCTGCCGTGGGCGATGCTTTTACACTCCGGCt                                                                                                                                                                          <  2:409848/107‑1 (MQ=255)                                                                                                                                                                                                                    ccagTTATCTCCCGTTGGAATTACTTGACCCGGGCGACAGCACGCGGGTGATTTTTATTTCCCCCGCTTAAGACACCTGCCGTGGGCGATGCGTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGAGGACCCGCTACAGGTGCTCCAGCAgg                          <  2:298795/248‑1 (MQ=255)                                                                                                                                                                                                                       gTTATCTACCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGc                       >  1:370118/1‑251 (MQ=255)                                                                                                                                                                                                                              cccGCTGTGATTCCTTTCCCCGGGCGTCAGGACGCCGCTGAATTTTAGTTCCCCCGCTAAAGCCCCCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCTCTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGa                   <  1:339989/248‑1 (MQ=255)                                                                                                                                                                                                                                  atgtgATTACTTTACCCTGCCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCATCTGCCGTGGGCGATGCTTTTACCTTCCGGCTTTGCAGGTCATCCGTATATCCGCTTTGATCTTGTGGTCACCGAGCCGCTTTGCACTTTAACCACTTTCGGTAAACAAACCATTGTTGCTGAAAGCGAAAAACGCCCAGCGCCCACTGGTAACCCGTTACCGGCGCTCCAGCCGGCGCTGGATCgcgcc            >  2:108623/2‑250 (MQ=255)                                                                                                                                                                                                                                            tttaccCTGGCGTCAGGACGACGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGAACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGCGCTCCAGCAGGTGCTGAGTCGCGCAGACATTCGcc  >  1:180372/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      GGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGCAGGTGCTGGATCGCGCAGACATTCGCC  >  NC_000913/1894599‑1895084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑