Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,852,991	G→A	S330F (TCC→TTC)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,852,991	0	G	A	100.0%	62.1 / NA	21	S330F (TCC→TTC)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/13);  total (8/13)

GTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAA  >  NC_000913/1852746‑1853230                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                gtgtTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGcc                                                                                                                                                                                                                                             <  1:26010/250‑1 (MQ=255)       ataatGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAAc                                                                                                                                                                                                                                      >  1:130964/1‑251 (MQ=255)                   cGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGaaaa                                                                                                                                                                                                                          <  1:157351/251‑1 (MQ=255)                                                       gggCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTAcc                                                                                                                                                                                      <  2:130964/250‑1 (MQ=255)                                                                         aaCCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCACTAAATATTCCTACCGGAGCGCCAATCataat                                                                                                                                                                     >  1:192009/1‑250 (MQ=255)                                                                            cgcccAGGCGTAAATGCGTTGACAAAAGCCTCGGGATAAAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCAGAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGAGCAATAATTAATGCCGCAATAAATATGCCTACCGGAGCGCCAATCATAATAACa                                                                                                                                                                 <  2:297112/247‑1 (MQ=255)                                                                                                                               cgAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATg                                                                                                                                                                                                          >  1:14366/1‑159 (MQ=255)                                                                                                                               cgAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATg                                                                                                                                                                                                          <  2:14366/159‑1 (MQ=255)                                                                                                                                           acatGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCAt                                                                                                  <  1:18615/251‑1 (MQ=255)                                                                                                                                                     aaaGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACggagttgg                                                                                        >  2:198764/1‑245 (MQ=255)                                                                                                                                                         aagaTCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGtat                                                                                     >  2:114906/1‑250 (MQ=255)                                                                                                                                                              catcaCCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACAGCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCg                                                                               <  1:198764/251‑1 (MQ=255)                                                                                                                                                               atcaCCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCg                                                                               >  2:100334/1‑250 (MQ=255)                                                                                                                                                                                        cccACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGTTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTa                                                     <  1:344140/251‑1 (MQ=255)                                                                                                                                                                                                                    ataGCCTAACACTGCAATAATAATAAGGAAGGTGACACCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATAGTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAAAATGGTCGTTATCCATACGGTGATGGTAGAAAGCGAAATGTACATTGCAATTAAAACAGTAATCGCGACTAATGTCcgtcgtttcagtt                         <  2:126576/251‑8 (MQ=255)                                                                                                                                                                                                                         cTAACACGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCt                    >  2:91201/1‑251 (MQ=255)                                                                                                                                                                                                                          acacaaggcgataaaaaaaagtaagGTGAAGCCAAATAAACGACGAGGAAAATGATCCATAATTAATGCCGCAATATATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCAGTAATATTGATTTATCGACATCAATGCTGGAGTTGACAAATATGGTCGGTATCCATACGGGGATGGTATAAAGTTAAATGTTCATTGCCATTAAAACAGTAATCGAGACTAATGTACGTCGTAACCTTTCACCtt                   <  2:118222/233‑1 (MQ=255)                                                                                                                                                                                                                             cacGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGAtt                                                                                                                                              <  1:311020/125‑1 (MQ=255)                                                                                                                                                                                                                             cacGGCAATAATAATAAGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGAtt                                                                                                                                              >  2:311020/1‑125 (MQ=255)                                                                                                                                                                                                                                     aaataataaGTAAGGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGc        <  1:100334/250‑1 (MQ=255)                                                                                                                                                                                                                                            aagtaagGTGAAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGaaa  <  1:256823/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                GTGTTAATAATGCCGCAACGCCATAGGGCGTGAAGACTGCGACGATCCGTCCGACGGCATTAACGAAACCCGAACCGCGCAGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAA  >  NC_000913/1852746‑1853230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑