Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,715	A→T	S377C (AGT→TGT)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,715	0	A	T	100.0%	51.6 / NA	20	S377C (AGT→TGT)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/10);  total (10/10)

GCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTAC  >  NC_000913/1776483‑1776928                                                                                                                                                                                                                                         |                                                                                                                                                                                                                      gCGTAAAGGCGCGGTGTGGAAGGTCGGCGTCGGTATTGCTGACGGTATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCCGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGAcc                                                                                                                                                                                                     <  2:64482/251‑1 (MQ=255)              gTGGGGAAGGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGt                                                                                                                                                                                        <  2:14183/251‑1 (MQ=255)                       gTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGc                                                                                                                                                                                >  1:190789/1‑250 (MQ=255)                                                        gTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTgg                                                                                                                                              >  2:177337/1‑251 (MQ=255)                                                                    gaagGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGCATACCCGTGCCATTCTGGATATGACGTCCCAGTTTAATTTTTATCACGGTGGCGGTCTGGCTGTTTGTTCTTTGTGTTTTGCTGAATTCCACTAGCACGGTGACTTCGGCGTGCATAAATTCAATGGTTAAATCCTGTGCACCGGTGGATTTAtttattt                                                                                                                                  >  2:93596/1‑246 (MQ=255)                                                                    gaagGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGatat                                                                                                                                  <  1:177337/251‑1 (MQ=255)                                                                      agGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGCTTGGCGGAATTACTTCACCGGGTATCGCCTTTGGCGCGAACGTGCATACCCGTGCCCTTCTGGCAATGAAGTCCCAGTTTGATTTTTATCACGGGGGAGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGACGACAAGCACGGTACCGTCGGCGTGACTAAATTCAATGGTAAAATCATTGGCACCGGTGGATTTGTTGatttc                                                                                                                                 >  2:147532/1‑247 (MQ=255)                                                                         ttGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTg                                                                                                                             >  1:302332/1‑251 (MQ=255)                                                                           gtgcagtggaCTTTAATCGGACGGGAGAAACAGGGCCGATTGGAGGAATTACTTCACAGGGGATCGCCTTTGGCTCGAACGTGAATACCCGTGCCATTCTTGATATGACTTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTATCGTCGGCGTGCATAAATCCAATGGTAAAATCATGGGCATCGGTTGATTTATTGATATCAGTGcc                                                                                                                           <  2:128163/243‑1 (MQ=255)                                                                                   ggcTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCgaa                                                                                                                   <  2:51566/249‑1 (MQ=255)                                                                                                     gAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCACAGTTTGATTTTTATCACGGTGGCGGGCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGAAAGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGAACCGGTGGATTTATTGATATCAGTGCAAATTCGAAGAACATCAttttatgggg                                                                                                 >  1:226223/1‑245 (MQ=255)                                                                                                                   tGGCGGAATTCCTTCACAGGGGATCGCCTTTGGCGCGAACGGGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCGGCGGCACATTAACTGCg                                                                                    <  2:374291/250‑1 (MQ=255)                                                                                                                    gggggAATTAATTCACATGGGATCGCCTTTGGCGCGAACGTGAATCCCCGTGCCATTCTGGATATGACGTTCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGGTATTTGTTATTTGCTGAAGTCGACCAGCACGGTAACGTCTGAGTGCATAAATTCAATGGGAAAATCATGGGCACCGGTGGATTTATTGATATCAGGGGCACTACGAAGAAAATCATTGTCTGCGGCACATTAACTGCgg                                                                                   <  2:25150/247‑1 (MQ=255)                                                                                                                          aTTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTAGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGttt                                                                            >  2:159907/1‑251 (MQ=255)                                                                                                                                                      cgAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAAtt                                                 >  1:365367/1‑250 (MQ=255)                                                                                                                                                                      tGCCATTCTGGCTATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTCTTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGGGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCCCATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCagga                                  >  2:63840/1‑249 (MQ=255)                                                                                                                                                                                                ttGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGa       <  2:190789/250‑1 (MQ=255)                                                                                                                                                                                                     ttttATCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTAc  <  1:13877/250‑1 (MQ=255)                                                                                                                                                                                                          tCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCgg                                                                                                                                                                                     <  1:236279/66‑1 (MQ=255)                                                                                                                                                                                                          tCACGGTGGCGGTCTGGATGTTTGTTATTTGTGTTTTGCTGAAGTCGACCAGCACGGTAACGTCgg                                                                                                                                                                                     >  2:236279/1‑66 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                      GCGTAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACGGTAGAAACAGGTCCGATTGGCGGAATTACTTCACAGGGGATCGCCTTTGGCGCGAACGTGAATACCCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAATTTATTCGGGAACTAC  >  NC_000913/1776483‑1776928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑