Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	64.4 / NA	20	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/13);  total (7/13)

ACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTG‑AAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGG  >  NC_000913/1652106‑1652569                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                aCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTG‑AAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGaaaa                                                                                                                                                                                                                        <  1:400873/251‑1 (MQ=255)    ggAGTTCGTCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTG‑TAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCCTTTCACGGTATCAAAAACACAGGCGGGAACCAAGCGGGTCATTCAGCGTAGTAAGTCAGCAATCGACGCGCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGGGCACCTCAGAGAGTATTGCAGAACATaaaac                                                                                                                                                                                                                       <  2:154475/249‑2 (MQ=255)       gTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGAACTG‑AAAAATGGAACAATGATGATCAGGAGAAAATTATCAAAAGACCGTGTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCTGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGAAGTTCACCTCAGAGAGTATGGGAGAACAGAAAAACaaaa                                                                                                                                                                                                                  <  2:22466/251‑1 (MQ=255)                            gCACTTGGATGGGAGGACATAGATCTG‑AAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑c                                                                                                                                                                                             <  1:48474/250‑1 (MQ=255)                                       ggaggaCATAGATCTG‑AAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAg                                                                                                                                                                                 <  1:257172/251‑1 (MQ=255)                                            aCATAGAGCTGTAAAAATGGA‑CAATGATGATCAGGATAAAATTAGCAAAAGACGGTTGCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAATCGACGCTCTTCGTAGTCAGATGACATTAACGAGACTGTGCAAATAGCATATCATTGATGTTCACCTCATAGAGTATGGCAGAACAGAAAAATAAAAATGCTCCTTTGTTTTTCAA‑TCTGAAGTGGCAGCgaga                                                                                                                                                                            <  2:409266/251‑1 (MQ=255)                                                                   aaTGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGa                                                                                                                                                           <  1:72622/246‑1 (MQ=255)                                                                   aaTGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAAAGGGGCATTCATTTTATTAAACCCGCAAACGGCGCTCTCCCGAGGCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTTCACCTCCGAGAGTATGGCAGAAAAAAAAAAAAAAAAATCACCCTTTTTTTTCAAACCT‑AAGTGTCAACGAGGGAaaaaaaattttgggaagaat                                                                                                                                                      >  2:72622/1‑237 (MQ=255)                                                                       atgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGATTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTAc                                                                                                                                                  <  1:297819/251‑1 (MQ=255)                                                                                                 ccGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTCAATCATTTTATTAAACCAGCAATCGACGCTCTCCGGGGGCAGATGAAATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAAAAAACATGCACCTTTGTTTTTCAA‑ACTGAAGTGACAGGGAGGGGAAAAAATTATGGTGACCATTTTACCGTTGGCTCAATTAGGCAGATGTggg                                                                                                                        >  1:402799/1‑251 (MQ=255)                                                                                                                 aaaaCACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCACTCCGGAGGCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCGCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACg                                                                                                        <  1:68249/251‑1 (MQ=255)                                                                                                                                                                             cGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCt                                            <  2:264168/251‑1 (MQ=255)                                                                                                                                                                                   cAGATGACATTAACAAGACTGAGCAAAGAGCATATCATTGCTGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGAGGGACGCAGCGATAAAACGGGACGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTCATGGTCGCTGACAGc                                      >  2:135831/1‑251 (MQ=255)                                                                                                                                                                                                     cTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCAtt                    >  1:49784/1‑251 (MQ=255)                                                                                                                                                                                                         gCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACt                                                             >  1:352661/1‑206 (MQ=255)                                                                                                                                                                                                         gCAAAGAGCAAATCATTGATGTTCACCTCAGGGAGGAATGCAAAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTAAGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACGCCGCCATCGAAAATCATATCAGTCGAGACATACt                                                             <  2:352661/206‑1 (MQ=255)                                                                                                                                                                                                           aaaGAGCCTATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAACATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTCTGCCTGCTGGTCGCTGACAGATGGTGCTAACCCGGCATTTATAGc              >  2:170905/1‑251 (MQ=255)                                                                                                                                                                                                                  atatCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAg       <  1:179107/251‑1 (MQ=255)                                                                                                                                                                                                                    atCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATACAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCAGCCAGCTGGTGCTAACCCGGCATTTATAGCAAACCAgct     >  2:271726/1‑249 (MQ=255)                                                                                                                                                                                                                       aTTGATGTTCCCCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATggg  <  1:150694/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                ACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTG‑AAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAA‑CCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGG  >  NC_000913/1652106‑1652569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑