Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,553,445	G→A	P132S (CCA→TCA)	adhP ←	ethanol dehydrogenase/alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,553,445	0	G	A	100.0%	100.3 / NA	34	P132S (CCA→TCA)	adhP	ethanol dehydrogenase/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/17);  total (17/17)

AGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATG  >  NC_000913/1553202‑1553667                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               ggTTAATCTCTAAATCTGCTCCCTCTGCGGTTGCCAGTTTTAACTGCCCATCATTGACATCAATGGCGATCACTGTGGGGTTAAAGTAATTCTCCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCTAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGTTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACtttt                                                                                                                                                                                                                         <  2:384637/250‑1 (MQ=255) aGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACtttt                                                                                                                                                                                                                         <  1:31417/251‑1 (MQ=255)  gTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGAACGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTa                                                                                                                                                                                                                        <  1:139006/251‑1 (MQ=255)     aaTCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCCCATCATTGACAACAATGGCGATCACTTTGGCGTTAAAGACATTCATCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTAcc                                                                                                                                                                                                                      <  1:22316/250‑1 (MQ=255)                  tcgcCCATTTCGGTTGCCAGTTTAAACTGCTCATCATTGACATCAATGGCGATCCCTTTGGCGTTAAAGACTTTCTTCGCGTATGGCAGGGCGAGGTTCCCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCgg                                                                                                                                                                                                            <  2:73869/246‑1 (MQ=255)                   cgcCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTACAGACCATCAGACACTTTTACCGCGTAATCGGCgccc                                                                                                                                                                                                       >  2:288633/1‑248 (MQ=255)                    gcCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGAc                                                                                                                                                                                                        >  1:162548/1‑249 (MQ=255)                    gcCCATTTCGGTTGCCAGTTTTAACTGCTCATAATTGACATCAAGGGCGATCACTTGGGCGTTAAAGCCATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGAc                                                                                                                                                                                                        <  2:162548/249‑1 (MQ=255)                       cATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACg                                                                                                                                                                                                    >  2:140618/1‑250 (MQ=255)                             ggTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCAc                                                                                                                                                                                              <  1:285657/250‑1 (MQ=255)                              gTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCActc                                                                                                                                                                                            >  1:71266/1‑251 (MQ=255)                                                 caacatTGACATCAATGGCGAGCACTTTGGCGTTAAAGACATTCTGCTCTTATGGCAGGGCGAGGTTACCCAGGCCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGGGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTGCCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCa                                                                                                                                                                         <  1:243515/248‑1 (MQ=255)                                                            tCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGAATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCAGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATcc                                                                                                                                                              >  1:16548/1‑251 (MQ=255)                                                               aTGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCCTCTGACACTTTTACCGCGTACTCGGCGACCACGATGCCCTCTTCCGCCATCCCGCCATCAACGCTGTCTCCGGc                                                                                                                                                           >  2:340012/1‑251 (MQ=255)                                                               aTGGCGATCACTTGGGTGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACACAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGACTTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACCGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCCTCATAGCTGTATCCgtc                                                                                                                                                           >  2:142080/1‑249 (MQ=255)                                                                             ggCGTTAAAGACATTCTTCGCGTATGGCAGGGCTGGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTACAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAAc                                                                                                                                             <  1:288633/251‑1 (MQ=255)                                                                                gTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGc                                                                                                                                          >  1:332787/1‑251 (MQ=255)                                                                                     aGACATTCTTCGCGTATTGCAGGGCGATGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGc                                                                                                                                     <  1:27210/251‑1 (MQ=255)                                                                                               cgcgTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATCGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCACCGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTAc                                                                                                                           >  1:149154/1‑251 (MQ=255)                                                                                                    aTTGCAGGGCGAGGTTCCCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTccgccatc                                                                                                                                                                                  <  1:184102/191‑1 (MQ=255)                                                                                                    aTTGCAGGGAGAGGTTATCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTccgacatc                                                                                                                                                                                  >  2:184102/1‑191 (MQ=255)                                                                                                                               cAAGACCGAAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCt                                                                                           <  1:84200/251‑1 (MQ=255)                                                                                                                                             gCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTa                                                                                                                   <  1:13142/213‑1 (MQ=255)                                                                                                                                             gCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGATGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTa                                                                                                                   >  2:13142/1‑213 (MQ=255)                                                                                                                                               aaTCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAAccacgcc                                                                            >  1:71803/1‑250 (MQ=255)                                                                                                                                               aaTCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAAccacgcc                                                                            >  1:270395/1‑250 (MQ=255)                                                                                                                                                   ccccgCCCTGGACGAATTTTTGGAAGATGACCGGCTTTTTAGGTGGTTACTCCCGCACATGTAAGGCTGCTGGCCCCCCCCGAGTCCAGACCATCTGACACTTTTCCCGCGTAATCGGCGACCCCGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATACGCAATGACCGCATCCTTCGTAGCACCTCGCCACGCt                                                                       <  2:57200/247‑1 (MQ=255)                                                                                                                                                                    ttttGAAAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGATGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGtt                                                      >  1:287702/1‑251 (MQ=255)                                                                                                                                                                                aaCGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAgggg                                           >  2:74399/1‑248 (MQ=255)                                                                                                                                                                                               tgtgACGCCCCCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTCCCGCGTAAACGGCGCCCACTATGCCCTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACt                            <  2:332787/249‑1 (MQ=255)                                                                                                                                                                                                  gACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCt                         <  1:190625/250‑1 (MQ=255)                                                                                                                                                                                                             aGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCCCTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCg             <  1:74399/251‑1 (MQ=255)                                                                                                                                                                                                               gTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCAGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTAACACTGATACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCAACACTGGCACGTTCGCCTGGTTTTAATGGGGAGACACCTGGACACACTACTGCCACCACACCGa            >  1:402786/1‑250 (MQ=255)                                                                                                                                                                                                                        ctgGCCGCCGCCGAGTCCAGACCATCTGACACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATg  >  1:160894/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               AGTTAATCGCTAAATCTGCGCCCATTTCGGTTGCCAGTTTTAACTGCTCATCATTGACATCAATGGCGATCACTTTGGCGTTAAAGACATTCTTCGCGTATTGCAGGGCGAGGTTACCCAGACCGCCAAGACCGTAGATAGCAATCCACTGCCCTGGACGAATTTTTGACAGCTTAACGGCTTTGTAGGTGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATG  >  NC_000913/1553202‑1553667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑