Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,400,978	C→T	G266S (GGT→AGT)	abgT ←	p‑aminobenzoyl glutamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,400,978	0	C	T	97.2%	115.2 / ‑3.7	36	G266S (GGT→AGT)	abgT	p‑aminobenzoyl glutamate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (21/14);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

CAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACCTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAACACGTCGGTTGTG  >  NC_000913/1400762‑1401219                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   cAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTc                                                                                                                                                                                                                  <  1:214366/251‑1 (MQ=255)        tAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCt                                                                                                                                                                                                           >  2:324559/1‑251 (MQ=255)           aTGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGGAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCa                                                                                                                                                                                                        <  1:70521/251‑1 (MQ=255)                       cGCCTGACGGCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTATGCAGTTTCTCATCGc                                                                                                                                                                                            <  2:68528/251‑1 (MQ=255)                                  gAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCt                                                                                                                                                                                  <  2:406638/249‑1 (MQ=255)                                  gAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCt                                                                                                                                                                                  >  1:406638/1‑249 (MQ=255)                                               aGCGATGTCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTGTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTGGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAACCCAAAACGCTGACTTTCGGTCAATTTCTGCAGTTTCTCATCGC‑GTTTGCCATGCCATTGACCTAAc                                                                                                                                                                    <  2:69533/250‑1 (MQ=255)                                                            gCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCgatgat                                                                                                                                                      >  1:252287/1‑251 (MQ=255)                                                                 cGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATAGACCTAACCGTGGCTCGATGATTTTGt                                                                                                                                                 >  2:280426/1‑251 (MQ=255)                                                                  gagaCAACAAAGAAAAAACGAATGATCCGTGGCACGATCCCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGAAGCGATACGACACTTGCTATGAGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGTTTTTGTc                                                                                                                                                >  2:62373/1‑251 (MQ=255)                                                                       aacaaAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGtt                                                                                                                                            >  2:81954/1‑250 (MQ=255)                                                                        acaaAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGCTTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTAt                                                                                                                                          >  2:174442/1‑251 (MQ=255)                                                                            aGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAgg                                                                                                                                      >  1:62338/1‑251 (MQ=255)                                                                             gAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGgc                                                                                                                                     >  2:323854/1‑251 (MQ=255)                                                                                   aaGAATGATCAGGTGCACGATACCGTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTTACTTTCGGTCAATGTCTGCAGGTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCcaa                                                                                                                               <  1:359356/251‑1 (MQ=255)                                                                                     gAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCcaaca                                                                                                                             <  1:379262/251‑1 (MQ=255)                                                                                         gATCAGTGGCACGATACCTTTACTAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGTAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTGTGTCGGTTATCAGGCCGCCAACAATCg                                                                                                                         >  2:279160/1‑251 (MQ=255)                                                                                                     gATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCCGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACt                                                                                                              >  2:316644/1‑250 (MQ=255)                                                                                                            ttAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCACCAATCGTCAGTACGACTACGGAGCt                                                                                                      >  2:378455/1‑251 (MQ=255)                                                                                                                 aaaGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGcc                                                                                                  >  1:133296/1‑250 (MQ=255)                                                                                                                   aGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAAAGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCCTTGACCTAACCGTGGCTCGATGATTTTGTCGGTAATCAGGACGCCAACCATCGTAAGTACGACTACGGAACTGGCCac                                                                                                >  2:61622/1‑249 (MQ=255)                                                                                                                              aTCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGt                                                                                                                                             >  1:212114/1‑194 (MQ=255)                                                                                                                              aTCACGGTGTAATTAATCGGACCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGt                                                                                                                                             <  2:212114/194‑1 (MQ=255)                                                                                                                                cACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGGGCTGGCCATAAAATACCAGTTAt                                                                                  >  2:106952/1‑251 (MQ=255)                                                                                                                                cACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTa                                                                                   <  1:323854/250‑1 (MQ=255)                                                                                                                                  cGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCa                                                                                <  1:378455/251‑1 (MQ=255)                                                                                                                                              tCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACg                                                                    >  1:170941/1‑251 (MQ=255)                                                                                                                                                ggATAGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTAGCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTg                                                                  <  2:417942/251‑1 (MQ=255)                                                                                                                                                                   tttGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCCGTTATCAATTACACTGACGTGCATTTGCGGAttaaaaga                                                >  2:370655/1‑244 (MQ=255)                                                                                                                                                                              aCCATCAGCGCAATCGCAGCAATAAAAAGAAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACATAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACTGAGCTGGCCATAAAATACCAATTATCAATTACACTGACGTGCATTTGCGTATTGCACGCAGCTGCCGCTTc                                    >  1:276820/1‑251 (MQ=255)                                                                                                                                                                                cATCAGCGCAATCGCAGCAATAACAAGTAGCGATACGACACCTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCCACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTCCCCTGACGTGCATTTGCGGATTGAACGCAGCTGCCACTTCCg                                  >  2:138901/1‑251 (MQ=255)                                                                                                                                                                                         aaTCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTcccgaacggc                           >  2:145218/1‑241 (MQ=255)                                                                                                                                                                                         aaTCGCAGCAATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTcc                                   <  1:145218/241‑1 (MQ=255)                                                                                                                                                                                                gcaATAAAAAGTAGCGATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAg                  <  1:324559/251‑1 (MQ=255)                                                                                                                                                                                                               gATACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAACCAGTCGGTtgg   >  2:309092/1‑250 (MQ=255)                                                                                                                                                                                                                aTACGACACTTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAACACGTCGGTtgtg  <  1:26979/251‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   CAATCATTAAATGCGGTAAATCCGCCTGACGTCGAATTGTGCGGGTAGCGATGCCATAAGCCAGCGAGACAACAAAGAAAAAAAGAATGATCAGTGGCACGATACCTTTAATAAAGGGTGATGGCATCACGGTGTGATTAATCGGATCGCGCAATATCCCGTTTTGCGGGATCACCATCAGCGCAATCGCAGCAATAAAAAGTAGCGATACGACACCTGCTATGCGTAAACCAAAACGCTGACTTTCGGTCAATGTCTGCAGTTTCTCATCGCTGTTT‑CCCTGCCATTGACCTAACCGTGGCTCGATGATTTTGTCGGTTATCAGGCCGCCAACAATCGTCAGTACGACTACGGAGCTGGCCATAAAATACCAGTTATCAATTACACTGACGTGCATTTGCGGATTGAACGCAGCTGCCGCTTCCGTGCTGATCCCCGACAGCAACACGTCGGTTGTG  >  NC_000913/1400762‑1401219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑