| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,859 | 0 | T | C | 88.9% | 82.8 / ‑4.8 | 36 | Y110C (TAT→TGT) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (17/15); minor base A (2/2); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGA > NC_000913/1358628‑1359086 | ccTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACCCCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCa < 1:87072/251‑1 (MQ=255) tATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTAGTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCAt < 2:345666/250‑1 (MQ=255) gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGAAAAAGCAGGGTATTGAAGTTAGCGCCCAGGTTGAAGCTCTTCAGATTCGCGGCGGGTACAAATG‑CGACGAA‑TG‑ATACCACCCACACCCACGGCGCCACTTACGGGAAACAGGG‑GGGGAGATAGAATTTCGGCAACAAAACGATAATCATCGGCAAAAAAAGACAATGCAGCAGC‑GATGAACAGCCGACCATAAAACC‑GACggcc > 1:267704/1‑248 (MQ=255) aaCTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGAATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGGCGAAAGG‑TAACC‑CCCACACCCCAGGCGGGACTTCCGGGAC‑ACGGGCGGGGAGAAAGATTTTCGCCAACAAGACGGTAAATAACGGCAAAAAGAGAAAACCCCGCCGC‑GATGACCAGCCGACCATAAAtaccaagaggg > 2:130917/1‑241 (MQ=255) aCTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCgg < 1:226704/251‑1 (MQ=255) cTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAAAGACAATCCAGCAGC‑GATGACCAGCCGACCCAAAATCC‑GACGTGCggg > 1:15733/1‑251 (MQ=255) aaGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAAAGGCGACGAA‑GG‑GTACCACCCAAACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGGGAGATAGATTTTCGCCCACAAGACGGTAATCATCGGGCAAAAGAGACAATCCAGGCGC‑GATGACCAGACGGCCATAAATCC‑GCCGGGCGGGGTAATCg > 1:42934/1‑250 (MQ=255) tAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACAAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATTGCGACGAA‑GGGTAACCACCC‑CACCCACGGCGGGACTTTCGGGGA‑GAGGGCGGGGAGGAAGATTTTCCCCCACCAGACGTTTATCCTCCGCCAAAAGAACAAATACAACAGCCGA‑GACCAGCCGACCAAAAAtacctcggtgagggtgaacaacttt > 2:157369/1‑227 (MQ=255) caTGATGGAGATTTGCACCAGTACAAACAGGGTATTTAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAgg < 1:77897/251‑1 (MQ=255) tgatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGGA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCa > 1:292914/1‑210 (MQ=255) tgatgGAGAGTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCa < 2:292914/210‑1 (MQ=255) tgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCCCTTCCGGGGA‑GAGGGCGGAGAGATAGATTTTTGCCAACAAGACGTTTATTATCGGCAAAAAGAAACAATCCAGCAGC‑GGAGAACAGCCGACCATAAATCC‑GCCGTGCGGGTTAATCGACTTTTTcgcggcagtat > 1:99340/1‑242 (MQ=255) agaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGGA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGGTAGATTTTTGCCAACAAGACGTTAATCCACGGCAAAAAAGGACAAACCAGCAGA‑GATGACCAGCCGACCAAAAATCC‑CACGGGCGGGGTAAACGCCTTTTGCGCGTAGGGATAggac > 1:313885/1‑249 (MQ=255) tACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCa < 2:289593/183‑1 (MQ=255) tACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑GTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCa > 1:289593/1‑183 (MQ=255) cAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGa < 2:42934/251‑1 (MQ=255) aaaCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGGCGAA‑GG‑GTACCACCCACCACCACGGCGGCACTTCCGGGGA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATACAGCAGC‑GATGACCAGCCGACCATAAATCCAGA‑GGGCGGGTTAATCGACTTTTGCGCGGAGGTATAGGCCGAACCGGCCCCCGGa > 1:257284/1‑250 (MQ=255) aaaCAGGGTATGGAAGTTAGCGATCAGGTTGACGCGCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTGAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAAGCGTCTTTTGAGCGTAGGTATAGGCCGAACCGGCCTCCGGaa < 1:34913/251‑1 (MQ=255) aTTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGTCCTCCGGAAACTGGCGa < 1:350247/250‑1 (MQ=255) cGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTT‑AAAAGGCCCAGAA‑GG‑TTACCACCCCCACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGGGGTAGGATTTCGCCAAAAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GAGGACCAGCCGACCATAAATCC‑GACCGGCGGGGTAATCGACTTTTGCGCGTTGGTATAGGCCGAACCCGCCTCCGGAAACTCGAGACCCCGTTTGccc > 2:30411/1‑250 (MQ=255) tCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGc‑ac < 1:206053/250‑1 (MQ=255) ggcggTTA‑AAATGGCGACGAA‑GG‑TTACCACCCCCACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTCATCGCCTTTTGCGCGTCGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGc > 2:370248/1‑221 (MQ=255) ggcggTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGc < 1:370248/221‑1 (MQ=255) ggTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GGGGGCGGAGAGATAGCTTTTCGCCAACCAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GCTGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGTCGAACCCGTTTGCCGTAGCTGATAGCGGTAAACAGC‑CCACCcgccc > 2:37729/1‑250 (MQ=255) ggCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGC‑ACACCCgccagcgccagc > 1:172633/1‑250 (MQ=255) acgaA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAAt > 1:101651/1‑250 (MQ=255) ttACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACAAGCCGACCATAAATCC‑GACGTGCGGGTCAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAATAGGACGcc > 1:260077/1‑251 (MQ=255) cacccacacccaCGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACAATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAAGCCGAACCGGCCTCCGGACACTGGCGAACCAGTTTGCCGTAGCTGATAGCGATAAACAGC‑ACACCCGCCAGCGCCAGAAAATAGGACGCCGGa > 1:162374/1‑250 (MQ=255) gcccTTCGGGGAA‑GGGGGCGGATATATAAATTTTCGCCAACAAGACGTAAACCATCGGCAAAAAGAGACAATCCAGCGGC‑GATGCCCAGCCGACAAAAAACCC‑GACGGGCGGTTTAATCCTCTTTTGCGCTTAGGTTTAGGCCGAACCGGCCCCCGGAAACTGGCGACCCAGTTTGCCGAAGTGGATAGGGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATg < 1:262498/248‑1 (MQ=255) gcACTCCCGTGAA‑GAGGGCGGAAAGATAGATTTTCGCCAACAAGCCGTTAATAATCGGCAAAAAGAGACAATCCAGCAGT‑GAGCACCAGCCGACCATAAATCC‑GACGTGCGGGTTATTCGACTCTGGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGGGACCCATTTTGCCGTAGCTGATAGCGTTAAACACCTAC‑CCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTTACCGTCGCTAATg < 1:244860/251‑1 (MQ=255) aCTTCCGGGAA‑GAGGGCGGAGAGATAGATTTCCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACAAAAAATCC‑GACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAAACGGGCGAACCAGTTTTCCGTAGCTGATAGCGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGcc < 1:30411/251‑1 (MQ=255) ttCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGATAATCGACTTTTGCGCGTAGGAATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAATAGGCCGCCGGAACGTGCCCGTCGCTAATGCCgg > 2:230114/1‑251 (MQ=255) agGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGACCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGC‑ACACCCGCAAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGCGTCAATAcc > 1:200934/1‑251 (MQ=255) cccaacaggacttaaaacatcGGCAAAAAGACACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACGGGCGAACCAGTTTGCCGTAGCGGATAGCGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCAt < 1:235297/234‑1 (MQ=255) aaaaagccGTTAAACATCGGAAAAAAGAGAACATCCAGCATC‑GAGGCCCAGCCGACCAAAAATCC‑GACGGGGGGTTTAATCGACGTTTGCGCTTAGGTATAGCCCGACCCGGCCTCCGGAAACGGGCGACCCAGTTTGCCGTAGCTGATAGCGGTAAACACC‑CCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCgg < 2:348067/244‑1 (MQ=255) cGTTAATACTCGACAAAAAGAAAAAACCCAGTAGC‑GAGGACAAGCCGATCAAAAATCC‑GACGGGCGGTTTAATCGTTTTTTGCGCGTAGGTATAGGCCAAACCGGCCTCCGGAAACTGGAGACCCAGTTGGCCGTAGCTGATAGCGGTAAACAGC‑ACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTgaga < 1:242754/251‑1 (MQ=255) | CCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTA‑AAATGGCGACGAA‑GG‑TTACCACCCACACCCACGGCGGCACTTCCGGGAA‑GAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGC‑GATGACCAGCCGACCATAAATCC‑GACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGC‑ACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGA > NC_000913/1358628‑1359086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |