Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,322,325	G→A	L208F (CTC→TTC)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,325	0	G	A	100.0%	101.8 / NA	35	L208F (CTC→TTC)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

ATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAA  >  NC_000913/1322083‑1322568                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aTTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACTCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACgttgtt                                                                                                                                                                                                                                             <  2:2066/251‑1 (MQ=255)   tACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGttt                                                                                                                                                                                                                                            <  2:226344/250‑1 (MQ=255)          tttCAGCACGGAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTttcttct                                                                                                                                                                                                                                     <  2:81918/250‑1 (MQ=255)           ttCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATAGCTTTTTGCAACAAACACACTACGACACCGAACTCTTCATCGCTCTGATTACATTAACAACGCATATGCGGCACGGAAACCACTGGCAGCGGAGGCGCGGCTTCGGTCAGTTGCTGAAGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTttcttcttc                                                                                                                                                                                                                                   >  1:173069/1‑251 (MQ=255)            gcAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTttcttcttc                                                                                                                                                                                                                                   <  1:84488/249‑1 (MQ=255)               gCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTc                                                                                                                                                                                                                               <  1:61620/251‑1 (MQ=255)                    tAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTAGTTCAGGCGAGCAGGGAAACGTTGTTTTTCTTCTTCCTTCGGAGc                                                                                                                                                                                                                          >  2:136453/1‑251 (MQ=255)                      aTAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCCGTTGCTGACGTAGTTCGTTCAGGCGAGCAGGGAAACGTTGTTTTTCTTCTTTATTCGGAGCa                                                                                                                                                                                                                         >  2:162021/1‑250 (MQ=255)                          gccgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAg                                                                                                                                                                                                                    <  1:268396/251‑1 (MQ=255)                            cgccAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGc                                                                                                                                                                                                                  >  1:163026/1‑251 (MQ=255)                                      gACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAAGTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAAt                                                                                                                                                                                                        <  2:163026/251‑1 (MQ=255)                                       aCGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATa                                                                                                                                                                                                       <  1:312845/251‑1 (MQ=255)                                                 cagAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCttt                                                                                                                                                                                             <  1:127675/251‑1 (MQ=255)                                                              cGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGCAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTc                                                                                                                                                                                >  2:78167/1‑251 (MQ=255)                                                                      caccacCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTCCATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGCTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAAtaccc                                                                                                                                                                         >  1:270951/1‑246 (MQ=255)                                                                                    tttCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTc                                                                                                                                                                                <  1:141458/229‑1 (MQ=255)                                                                                    tttCACCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGACTACGGGTGCTTTTTTTCTGATGGTc                                                                                                                                                                                >  2:141458/1‑229 (MQ=255)                                                                                                    cGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAAt                                                                                                                                          >  1:157734/1‑251 (MQ=255)                                                                                                            gCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATGCACAACGCATATGCGGCACGGAAACCACTGTCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATGCGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCa                                                                                                                                   <  2:417376/250‑1 (MQ=255)                                                                                                                 aaaCGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTAtt                                                                                                                             >  1:367037/1‑251 (MQ=255)                                                                                                                      cACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCg                                                                                                                        <  1:136453/251‑1 (MQ=255)                                                                                                                           cGCCCCCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGAc                                                                                                                   <  2:354496/251‑1 (MQ=255)                                                                                                                              cACCGAACTCGTCATCGCTCTGATTACAGTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCGTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTGTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATCCGGGTGCTTTTTTTCTGATGGTCAATCTCCATCATCGTTTCAGCGAGATAAAAACAGAAATCAGGTCAGTTATTTTCCGCTGACAGt                                                                                                                <  1:414436/251‑1 (MQ=255)                                                                                                                                             ctctctGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGTTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCtt                                                                                                   <  2:258154/247‑1 (MQ=255)                                                                                                                                                                          ggCACGGAACCCACTGGCGGCGGCGGCGCGGCGTCGGTCTGTTGCTGACGTAGTTCGTCCAGGCGAGAAGTGAAACTTTTTTTTTCTTCTTCATCCGGAGCAAACAGGTTGGCATGAATACGGGTTTTTTTTTTCTGATGGTCAATCACTACCAGCTTTTCAGGGAAATAAAAACAGAAATCAGGGTATTTATTTTCCGCTGAAAGTTGCGTAAAATCTCCAAATCCCGCCACAAGGTCATAAGAGAACAg                                                                    <  1:78167/251‑1 (MQ=255)                                                                                                                                                                          ggCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGaaacg                                                                    >  2:314281/1‑248 (MQ=255)                                                                                                                                                                                gAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCCGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTcc                                                                                                                         >  2:186717/1‑192 (MQ=255)                                                                                                                                                                                gAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTcc                                                                                                                         <  1:186717/192‑1 (MQ=255)                                                                                                                                                                                    ccACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTCGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACTAGGTCATAAGAGAACAGGCCGCCgaa                                                           >  2:287271/1‑250 (MQ=255)                                                                                                                                                                                       cgggcggcggcggcgGGGCTGCGGACAGTTGCTGACGTTGTCCGTTCAGGCGAGAAGTAAAACTTTTTTTTTCTTCTTCATGCGGAGCAAACAGGCTGGCCTGAAGAGGGGTTCTGTTTTTCGGTGGGTCAACCCCCATCGGCGTTTCGGCGAAAAAAAAACAAAAAGGAGGTCAGTTTTTTTCCGTTTACAGTGCCGGTAAATCTCAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAAc                                                       <  1:52098/245‑1 (MQ=255)                                                                                                                                                                                           cAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGGATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGCAAGCAGGGCAGTTATTTTCCGCTGACAGTTGAGGGAAATCTTCCAATCCCGCCACACGGTCATAAGAGAACAGGCCGCCGAAGAAAATgg                                                   >  2:211516/1‑251 (MQ=255)                                                                                                                                                                                                   gcgcggcTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCg                                                                                          <  2:100108/204‑1 (MQ=255)                                                                                                                                                                                                   gcgcggcTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCg                                                                                          >  1:100108/1‑204 (MQ=255)                                                                                                                                                                                                                                        gCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAAc      >  2:113289/1‑251 (MQ=255)                                                                                                                                                                                                                                              aGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGaa  >  2:385756/1‑249 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ATTACTCTTTTTCAGCACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAA  >  NC_000913/1322083‑1322568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑