Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,310,770	A→G	H32H (CAT→CAC)	yciI ←	protein YciI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,310,770	0	A	G	100.0%	112.4 / NA	31	H32H (CAT→CAC)	yciI	protein YciI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/13);  total (18/13)

ACACGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGG‑TTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTG  >  NC_000913/1310527‑1311006                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             acacGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGT‑TTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTa                                                                                                                                                                                                                                       <  1:249145/251‑1 (MQ=255)               aCCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGG‑TTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCt                                                                                                                                                                                                                         >  1:247735/1‑251 (MQ=255)               aCCGTGCCCTTAAATATTGAATCTCTATTCAGAAAACTTTCTTAAATGG‑TTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTAAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCAGGCAATGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAACCGTGCt                                                                                                                                                                                                                         >  2:383435/1‑251 (MQ=255)                        ttAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGG‑TTTCACTGAAACGTGTTCATAGACTCCTGCCGCTATGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTAATCGTGCAGTAACTGTAAACGTGCTAAATGAGcc                                                                                                                                                                                                                >  1:417778/1‑251 (MQ=255)                            atatTGAATACCTATCCAGACCACTTTCT‑AAAGGGCTTGCGCTAAAACGTGCCCATAGACTCCTGACGCTACGTACGGGGCAGCATCGGCCCAGGCCTCAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAACCCCTGGATCGTTACTGTCTACCGCTGTCATTGGACCAGCTGTCAACAAACGACCTGCATCGTGCAGTAACTGTAATCGTGCTAAATGTGCCGGAc                                                                                                                                                                                                            <  2:415071/251‑1 (MQ=255)                                        aaTTCAGACAACTTGCTTAAAGGG‑GTTCAAGGAAACGGGTTCAAAGACACCTGCCGCGACGTACGGGTCAGCATCGGCCCATGCCGGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGGAAACGTGCTAAATGTGCCGGACGAACGGAAAGGc                                                                                                                                                                                                <  2:243869/250‑1 (MQ=255)                                                            aTGG‑TTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGaa                                                                                                                                                                                                    >  1:253597/1‑227 (MQ=255)                                                                         aaaCGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGATGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAAAGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGCGGCTATCAGCTTTGTCTTGAGCg                                                                                                                                                                >  1:258872/1‑251 (MQ=255)                                                                                ttCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTACACGTGCTAAATGTGCCGGCCGAACGGAAAGGCGTTTTTCGTGGCTATCAGCTTTGTATTGAGCGTAAATa                                                                                                                                                          >  2:386977/1‑250 (MQ=255)                                                                                  cATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAAc                                                                                                                                                        <  1:262677/250‑1 (MQ=255)                                                                                   aTAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACAt                                                                                                                                                      >  1:178360/1‑251 (MQ=255)                                                                                    tAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAACTAACAt                                                                                                                                                      >  1:198016/1‑250 (MQ=255)                                                                                    tAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCACTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTg                                                                                                                                                                    >  2:361935/1‑236 (MQ=255)                                                                                    tAGACGCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTg                                                                                                                                                                    <  1:361935/236‑1 (MQ=255)                                                                                            tGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACgg                                                                                                                                             >  2:84100/1‑251 (MQ=255)                                                                                                                 aTCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGaa                                                                                                                        <  1:134667/251‑1 (MQ=255)                                                                                                                     gCCCAGGCCTGCGCTGCTCCCAGCGATTCAAATTCAGCAATAACTGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGGCTACCGCTGGCATTGGACGAGCTGTCAACAAACGACCTTCATCGGGCAGTAACTGTAAACGTGCTCAATGTGCCTGACGAACGGAAAGGCTTTTTTCGAGGCTATCAGCTTTTTCTGGAGCGTAAACAACATACAACTCAGTCATATCTCCTTTTTCGGGAAAGtt                                                                                                                    <  2:363586/251‑1 (MQ=255)                                                                                                                                             gATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCg                                                                                                                            <  2:254344/219‑1 (MQ=255)                                                                                                                                             gATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCg                                                                                                                            >  1:254344/1‑219 (MQ=255)                                                                                                                                              aTTCAAATTCAGCCATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCg                                                                                           <  2:178360/251‑1 (MQ=255)                                                                                                                                              aTTCAAATTCAGCAATCACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCg                                                                                           >  2:300954/1‑251 (MQ=255)                                                                                                                                                tCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGa                                                                                          <  1:375826/250‑1 (MQ=255)                                                                                                                                                      tCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAg                                                                                                                      >  1:316084/1‑216 (MQ=255)                                                                                                                                                      tCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAg                                                                                                                      <  2:316084/216‑1 (MQ=255)                                                                                                                                                              aaCGGTGGAGCCAGTAAATCCCGCAGCGCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGaa                                                                           <  2:277829/251‑1 (MQ=255)                                                                                                                                                                 ggTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGaa                                                                           >  1:164620/1‑248 (MQ=255)                                                                                                                                                                       gCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTAGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGt                                                                  >  1:233362/1‑251 (MQ=255)                                                                                                                                                                               aTCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTa                                                          <  1:84100/251‑1 (MQ=255)                                                                                                                                                                                                           tACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGCAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTAc                              >  2:273855/1‑251 (MQ=255)                                                                                                                                                                                                            aCCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCACTGCATAAAGTAAGGGCAATTACg                             >  2:128570/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaCGACCTTCATCGTGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCActgatcctg  >  2:61859/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             ACACGGTTGCAAAAACCGTGCCCTTAAATATTGAATCTCTATTCAGAACACTTTCTTAAATGG‑TTTCACTGAAACGTGTTCATAGACTCCTGCCGCTACGTACGGGTCAGCATCGGCCCAGGCCTGAGCTGCTTCCAGCGATTCAAATTCAGCAATAACGGTTGAGCCAGTAAATCCCGCAGCCCCTGGATCGTTACTGTCTACCGCTGGCATTGGACCAGCTGTCAACAAACGACCTTCATCATGCAGTAACTGTAAACGTGCTAAATGTGCCGGACGAACGGAAAGGCGTTTTTCGAGGCTATCAGCTTTGTCTTGAGCGTAAATAACATACAACACGGGCACAACTCCTTGTTCGGGAAAGTTGTAAAGTACGTTATTTGAAAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTG  >  NC_000913/1310527‑1311006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑