Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	37.6 / NA	16	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/12);  total (4/12)

GGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCA  >  NC_000913/1306501‑1306957                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              ggggACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTg                                                                                                                                                                                                                 <  1:161616/250‑1 (MQ=255)          tgatgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATc                                                                                                                                                                                                       >  1:51755/1‑251 (MQ=255)                               ccTACATCCTTACACCAGGGCATTGATGTCGGCATTCCCCATACCGGATTCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGCCGCCGATCACCCCGCCTCCCGGTTGGGTTTGCCGTTCCCGTGGCCCGATTGCCGGTCCAGAGGGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGCCACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGc                                                                                                                                                                                  <  2:323988/250‑1 (MQ=255)                                  ccaccctacccccAGGGCATCGATGTCTGCAGTCCCCATACCTGACCCGAATCCGGAGAAGAACAAAACCATCCAGTTCCTGGAAGGGGAATTACCGTCGCCGACCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAACCACGTCCTGTCCTGGAGTGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAATCCAATAAGGGCTGa                                                                                                                                                                               <  2:175495/240‑1 (MQ=255)                                         tACACCAGGGCTTTAATGGCGGCACTCCCCACACCTGATCCGGATCAGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGGCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGtt                                                                                                                                                                          <  1:124125/249‑1 (MQ=255)                                                                       aTACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGAGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAACCAATAAGGACTGACAGTTGTCAGCCCTTTTTCACGCTACAAGCGattatt                                                                                                                                          >  1:416839/1‑251 (MQ=255)                                                                          ccTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGCCGCCGATCAACCCGCTTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttatttat                                                                                                                                       <  1:134912/251‑1 (MQ=255)                                                                             gagccGGATCTGGAGAAGAACAAACCCATCCATTTACTGGAAGGGGAATTACCGTCGCCGATCATCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCTATTGCCGGGCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGAGCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTc                                                                                                                                     <  2:112020/247‑1 (MQ=255)                                                                                  ggtttggtagaaataacacaccaccCATTTATTGGAAGGGGAATTACCGTCTCCTCTACACCCGCTTTCCGGTTGTGTTTTCCGTACCTGTGGCCCATTTGCCGGTCAAGA‑TGCCGCCAAACCACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTTCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGTTGACAGTTGCCATCCTTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCa                                                                                                                               <  2:332866/232‑1 (MQ=255)                                                                                            aagaaCAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCAGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGCCCGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATgg                                                                                                                     >  2:51176/1‑251 (MQ=255)                                                                                                                 cTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGAGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGACAGATGATATGGCAAAGTTTGTGATCTTTTTcg                                                                                                >  1:393065/1‑251 (MQ=255)                                                                                                                  gggaagttgAATTACCGCCGCCGATCAACCCGCTATCCGGTTGTGATTTCCGTACCCGTTGCCCGATTGCCGGTCCGGAGGCC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCGTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgc                                                                                               <  2:160075/243‑1 (MQ=255)                                                                                                                                            aaCCCGCCTTCCGGTTGTGTTTTCCGGACCCGGGGCCCGATTGCCGGGCCAGAGTGA‑GAAAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACa                                                                     <  1:359233/251‑1 (MQ=255)                                                                                                                                                        ggTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCa                                                         <  1:267059/251‑1 (MQ=255)                                                                                                                                                                                      gCCGGTCCAGAGTTC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAAACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATcacc                           <  1:359454/251‑1 (MQ=255)                                                                                                                                                                                                                gttcTGTACTGGAGGGGAGTTTACGACACGCCGTTTCGTGCCTGACAGTCTATTCGCGTTAAAAACAATAAGTGCTGCCAGTTGTCGGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCACCAGATGATAGGGCAAAGTTTGTGATCTTTTTCGGGGCATAACAGAATGCGGGCAAAAACATCGGTGAGTTCACCATCTTCACTGTCCTCCAGGCCAATCACCGCTTCGGCAAAAAAGTCCGGGTTCa  <  2:150944/248‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                              GGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCA  >  NC_000913/1306501‑1306957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑