Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	68.7 / NA	24	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

ATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGCGCAT  >  NC_000913/1169595‑1170082                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       aTGTACTTCAACCAGACGCGCACCGTTTGGTGCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCGGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCg                                                                                                                                                                                                                                               <  2:288176/251‑1 (MQ=255)         ccaCCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGAGTTGCTGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGCTATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGACTCGCATGATTGCCCATTGGGTTATGCGGTCCAGCCGGCACTAcc                                                                                                                                                                                                                                       >  2:178473/3‑251 (MQ=255)            ccAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCa                                                                                                                                                                                                                                    <  1:158990/251‑1 (MQ=255)                       ccGTTGGGTTCGGCAGATACTTTTATCGGAGTGTTGAGGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAAt                                                                                                                                                                                                                         <  1:128805/251‑1 (MQ=255)                                     agagACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATAGCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCTCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtat                                                                                                                                                                                                           <  1:235239/251‑1 (MQ=255)                                                   cagtcgtgcgccattccgatctGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATg                                                                                                                                                                                             <  1:319040/230‑1 (MQ=255)                                                    aGTGTTGATGATATTCAATTTGGTGCCTGGGGTGACGTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGCCGAATACAGCCAGCACTTACCCGCATGCCAATGCCGACATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAAGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTCACGCATGCACCTCAATTCCCTGTGCTTTCTAACGGGCGCGGATGt                                                                                                                                                                                            >  2:309854/1‑251 (MQ=255)                                                              gtatTCACTTTGGTGCCTGGGGCGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCACTGCCGAAATCGGCGTTCGTACCATGAAGAAAATAAACGCCGCCATGGGCCGCCAGACGAATCGCAAGATGGCCAATTGGGTTATCAGGTCCCGCCGGCACTACCGCAGACAACTCAATTTCCTGTGCCTTATACCGGGCGCGGGTGTTTGCCGTTgg                                                                                                                                                                                  >  2:84468/2‑251 (MQ=255)                                                                atTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGc                                                                                                                                                                                 <  1:99093/250‑1 (MQ=255)                                                                         ggTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCACTTCCCTGTGCTTTATAACGGGCGCGGATg                                                                                                                                                                                             >  2:319040/1‑229 (MQ=255)                                                                                                       ttttatatCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCACATAAACGCCGCCATAGGCCGCCCGACGACTCGCATGCTGGCCCCTTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCACCTCAATTCCCTGTGCTTTATAACGGGCGCGGCTGTTTGCCGTTGGCGGCCAGGTTGGGTTTGCCCGTTTGTCTGAACCAGTGGTc                                                                                                                                          >  2:51128/5‑250 (MQ=255)                                                                                                          gATATCGTCATCCGACGGAAGAATGCAGCCAGAATTTACCCGCATCCCAATGCCTAAATCGGCGTTCGAACCATGAAGCAAATAAACGCGGCCATAGGCCGCCAGACGAACCGCATGATGGCCCAGTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCGGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCATGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCAcc                                                                                                                                       <  2:13812/250‑1 (MQ=255)                                                                                                                gTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCg                                                                                                                                >  1:363422/1‑251 (MQ=255)                                                                                                                       cGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAGCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGGCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAg                                                                                                                         <  1:51128/251‑1 (MQ=255)                                                                                                                        gCAGACGAAAACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGGACCATGAAGAAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTGTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGc                                                                                                                        <  2:176167/251‑1 (MQ=255)                                                                                                                              gAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGGGTCAGCGTGTcc                                                                                                                  >  2:98928/1‑250 (MQ=255)                                                                                                                                                       gCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTg                                                                                         >  1:95206/1‑251 (MQ=255)                                                                                                                                                                aaaaTCGGCTGTCGCACCAGGAAGCAAATAAACGCTGCCACAGGCCGCCAGACTAATCGCATGATGGTCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCGGTGCTTTATAACGGGCGCGGATGTTTGCAGTTGGCGTCCAGGTTGGGTTTGCACGTTTTTCTGAAACGGTGGTCACCATTGTCGGTGGCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACgg                                                                                <  2:328251/250‑1 (MQ=255)                                                                                                                                                                               acaataaagaaaaaaaaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTAc                                                                 <  2:288215/237‑1 (MQ=255)                                                                                                                                                                                                              gccAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGc                                  >  2:254534/1‑251 (MQ=255)                                                                                                                                                                                                                                 gacccATTGGGTTATCCGATCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGGTGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGAAGTAATAGAGACGCGGCTCCGCAATGTTGATCACAATTCCt               >  1:93638/3‑251 (MQ=255)                                                                                                                                                                                                                                   cccATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACGCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACGGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTc             <  2:363422/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGTCGTCCAGGTTGGGTTTGCACGTTTTTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTtgcg          <  1:309854/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGGATTTTTACCCGGCGGGGAGTATTAGAGCCGCAGCTCCCCAATGTTTATCACAATGCCTTCGCGCGgcgcct  >  2:78147/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       ATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGCGCAT  >  NC_000913/1169595‑1170082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑