| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,061,935 | G→A | S46N (AGC→AAC) | torD → | trimethylamine‑N‑oxide reductase‑specific chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,061,935 | 0 | G | A | 90.3% | 67.3 / ‑6.4 | 32 | S46N (AGC→AAC) | torD | trimethylamine‑N‑oxide reductase‑specific chaperone |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (14/14); minor base C (0/3); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAG‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTG > NC_000913/1061692‑1062176 | gtggAAATGGAGAAGGACCACGGAACGGTGGAGCAGGTGACGGCGTTTAGCGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGGCGCAGGTGAAAT‑CATGACCACGCGGACAGCACAACAGATTGACTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAAACGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAAccg < 2:301903/251‑1 (MQ=255) aaTTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTCCGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCt > 2:202668/1‑251 (MQ=255) cGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGa > 1:179312/1‑250 (MQ=255) cGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGGGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGa > 1:64162/1‑250 (MQ=255) gAACAGTGGAGAGGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGGGCGAATATGTCCCCGCGTCGCAGGTGAAAT‑CATGCCCCCGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCGGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACg < 1:112381/251‑1 (MQ=255) aaaaGTGTAGCAGGGGCCGGCTTTAAACGGCCCGGGGGAGATGGTGGCGCAGGCCGAATATTTCCCCGCGCCGCAGGTGAAAT‑CATGCCCACGCTGACAGCACAAAAGATTGCCTGTTTTTAC‑CCTG‑GGCTGGCGCAGTTGTTCCCCCGTGAGCTGGAGGATAAACAACGGACGAAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCTTTGTTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGa < 1:385855/248‑1 (MQ=255) aGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACACCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAACA‑CGACACGCAGCTCACTGCGGCGGGGGACGAg > 2:299669/1‑249 (MQ=255) ggTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCg > 1:23770/1‑251 (MQ=255) aCGGTGTTTACCGGCCCCGTGGAGATGGTGGCGCAGTGCAAATATGTTCCCGCTTCGCGGGTGAAAT‑CATTCCCACGCTGACAGCACAACAGATTGCCTTTTTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGTGGGACGATGAACAACGGACGAAAATCGCCAGTGCACAGATGGCGGAA‑TGTTTTTCGTTGCTAAAAAC‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTAtt < 1:38331/251‑1 (MQ=255) gCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGGGGTGGaaaaacggaatgcc > 2:96655/1‑241 (MQ=255) gtggCGCAGTGCGAATATGTCCCCGCGGCGCAGGTGAATA‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGAAGTTGTTCTCCCGTGAGCTGGACGATGAACAATTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGcc < 1:39640/251‑1 (MQ=255) ggCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTgcggc > 2:410935/1‑197 (MQ=255) gCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAG‑CGAACCGCCGCTCACTGCGGCGGGGAACGCGCTGGAAAACCGGATTGCCACGCTGCCCGTAC‑GTGACGATTCCCGTCCGGAAc > 1:17573/1‑251 (MQ=255) atatGTTCCCGCGCCG‑AAGTGTAATACATGCCCTCGCTGACACCCAAACAGATGGCCTGTGTTTCCGCGTG‑G‑TTAGCGCAGTTTTTCTCCCGTGATCTGGAAGATGAACATCTGACGCAAATCGCCAGTGCGCAGTTGGCTAAA‑TGTTTTTCGTTG‑TGAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑c < 1:138272/251‑1 (MQ=255) cgcgTCGCAGGTAAAAC‑TATGCCCACGCTGACAGCAAAACAGATCGCCTGTGTTTGC‑TCTGTG‑CTAGCGCTGTTGTCCTCCGGTGAGCGGGAGGATGAACAACTGACTCAATTCGCCAGGGCGCGGAGGGCTGAA‑GGTTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACtt < 1:264648/251‑1 (MQ=255) gaGGTGAAAT‑CATGCCCACGCTGACAGCACAACAGTTGGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGGGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAT‑TGTTTTTCGTTGCTAAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGc < 1:145385/250‑1 (MQ=255) gACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGGGCTGGAAAAACGAATTGCCCCGGTGACAG‑ACAGGGACCAAGCCCGCCTTGAACCGG‑CCACGGACTTTTGCgggccgtttttcaagt > 2:109131/1‑237 (MQ=255) tGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGGATTGCCACGCTGACAGTAA‑GGGAAGATGCCCGGCTGGAACTGG‑CCGCGGGCTTTTGGGGCCGGTTTCtgttggccgccaa > 2:120619/1‑239 (MQ=255) acaacaacaGGTTGCCTGTTTTTCCCCCTG‑G‑CTGGCGATGTTGTTCCCCGGGGGGCTGGACAATAAACAACTGACACAAATCGCCAGTGCGCAGAGGGGTGAA‑GGGTTTTCGTTGCTAAAAAC‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAg < 2:127425/248‑1 (MQ=255) tccGCCTG‑G‑CTAGCGCAGTTGTTCCCCCGGGAGCTGGTCGAAAAAAAATTGACGCAAATCGCCAGTGCGAAGTTGGCTGAT‑TGTTTTTCGTTGCTGAAAAA‑CGACCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCgg < 1:62190/248‑1 (MQ=255) ccTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATTGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGGGAACGAGCTGGGAAACCGGATTGCCACGCTGACACTAA‑GTGGCGAATCCCGGCTGGAACTGGGCCG‑GGACTTTTTCGGCCTGGTTCTGATGACCGACAAAAAAGCGGCGCTGCCGTGTGGATCGGccca > 2:342537/1‑248 (MQ=255) g‑g‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGGCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAAAAAGCGGCGCGGCCGTATGACTCGGCCTACaa > 1:133319/1‑250 (MQ=255) ggcAGTTGTTCCCCCGTGAGCTGGAGGATGAACAATGGACGAAAACCGCCAGTGCGCAGAGGGCTGAA‑TGTTTTTGGTGGCTAAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGAc < 1:202668/250‑1 (MQ=255) cgcAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAAACGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGGGATGGAAAACCGTATTGCCACGCTGGCAGTAC‑GTGACGATGCCCGTCTGGAAAAGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAACCAAGCGGCGCCGCCGTATGCATCCGACTACaaccaagac > 1:274407/1‑244 (MQ=255) cAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGCCTGGGACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAACGGCGCTGCCGTATGCATCGGCCTACCAACAGGACg > 1:64686/1‑250 (MQ=255) tgtccccccGTGAGCTGGACGATGAACAACTGCCGAAAATCGCCAGTGCGCAGAGGGCTGAA‑TGTTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCATACAAACAGGACGAGCa < 1:120619/244‑1 (MQ=255) cGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGGATGCATCGGCCTACAAACAGGACGAGCAAGAGATTa > 1:221321/1‑250 (MQ=255) gggAGCGGGACGATGAAAAACGAACGAAAATCTCTGGTGCGCAG‑TGGGTGAAATGGTTTTCGTTGCAAAAAAA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACTGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTaaa < 1:27799/249‑1 (MQ=255) gctggaagtgaaacaccTGACGCAAACCGCGGGTGCGCAGAGGGCTGAT‑GGGTTTTCGTTGCTGAAAAC‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCt < 2:117084/240‑1 (MQ=255) gggaagATGAACAACTGACGCAAATCCCCGGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCAAAAAAA‑CAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTg < 1:389756/246‑1 (MQ=255) attgggtttgggtGCTAAAAAA‑CGATCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGACGTATGCATCGGCCTACAAACAGGACGAGAAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCg < 1:64417/239‑1 (MQ=255) gctaaaaaA‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTg < 1:344817/246‑1 (MQ=255) | GTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAAT‑CATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTG‑G‑CTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAA‑TGGTTTTCGTTGCTGAAAAG‑CGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTAC‑GTGACGATGCCCGTCTGGAACTGG‑CCGCGGACTTTTGCGGCCTGTTTCTGATGACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTACAAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATCATCTG > NC_000913/1061692‑1062176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |