Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,126	G→T	L277L (CTG→CTT)	appB →	cytochrome bd‑II subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,126	0	G	T	100.0%	102.3 / NA	34	L277L (CTG→CTT)	appB	cytochrome bd‑II subunit 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/22);  total (12/22)

CCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTAC  >  NC_000913/1039906‑1040369                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ccgaaCGGGCTGCAGTGTTGGTGATGCTGTGCTTGTTGCGGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTTGCCCAGGAGTCTAACGGTCCTTCCAATCCGTTACTGAAACTGGTGGCAGTGCTCCCTTGTTCCTGTATGAATAATTTTGTCGCGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCAGTGCTTACAGTGATGGCGATTTAGCTTGGTCGCCCg                                                                                                                                                                                                                       <  2:215101/248‑1 (MQ=255)      cgcgCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGGTACTGGCTGTGGGGTGGTATTGATGGCTTTTTAATGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGGAACTGGGGGCAGGGCTACCTGGGGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTTTGGGTCTTCCCGCTGCTGGGGTTCTTCTGCCCACTGCTTTCGGGGATTGCGATTTATCGGGGGCGCCCGGGTTg                                                                                                                                                                                                                  >  2:301682/1‑251 (MQ=255)       gcgcTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTgg                                                                                                                                                                                                                 <  1:319800/251‑1 (MQ=255)                 gTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGAttttt                                                                                                                                                                                                         <  1:214228/249‑1 (MQ=255)                      tGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGGTGAATAATTTTGTCGAGGCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTTATGGcc                                                                                                                                                                                                  >  1:120323/1‑250 (MQ=255)                       gATGCTGTGCTTTTTGCTGGCGGGTTTCTTGCTGTGGGTCGGTATTGATGGCTTTGTACTTCTCGCCCAGGGTGCTAACGGTCCTTCCCATCCGTTAATGGAACTGGTGGCAGGGCTACCTGGGGCCTGGGTGAATAATTTTGTCGAGTCGCCCGTTTTGTGGGTCTTCCCGCTGCTGGGATTCTTCTGCCCCTTGCTTACGGGGATGGCGGTTTATCGTGGGCGCCCGGGGTGGGGATTTTTTATGGcct                                                                                                                                                                                                 >  2:51648/1‑249 (MQ=255)                                  ttttGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCa                                                                                                                                                                                       <  1:291564/250‑1 (MQ=255)                                        tGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCggg                                                                                                                                                                                                                     <  1:359689/214‑1 (MQ=255)                                         tgCGGGTTACTGGCTGTGGGTCGGTATTGGTGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGc                                                                                                                                                                                <  2:129233/249‑1 (MQ=255)                                         ggggggTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTCCTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCg                                                                                                                                                                               <  2:314854/248‑1 (MQ=255)                                                   tGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGAGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGGTTTTTGGTGGCATCATTGCTGCAATTTGGCGGGATTTTCAc                                                                                                                                                                     >  2:324986/1‑251 (MQ=255)                                                                     gATGGCTTTGTCCTGCCCTCCCAGGAGGCTAACGGGCCTTCCAATCTGTTAATGAAACTGGTGGCAGTGCTACCTGGTTCCTGGATGAATAATGTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTTCGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGAGGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGtt                                                                                                                                                   <  2:349825/251‑1 (MQ=255)                                                                           tttGTACTGCTCGCCCAGGATGATAACGGTCCTTCCAATCCGTTAATGAAACGGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGATTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCt                                                                                                                                              <  2:306818/250‑1 (MQ=255)                                                                                      cGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGt                                                                                                                                  <  1:303982/251‑1 (MQ=255)                                                                                                              cAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGGCCTGTCGTCAAGAGTGTGTCCGATCTCCAGcc                                                                                                          >  2:70496/1‑251 (MQ=255)                                                                                                               aaTCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCt                                                                                                         <  1:324986/251‑1 (MQ=255)                                                                                                                      tAATGAAACTGGGGGCAGTGATACCTGGTGCCTGGATGAATAATTTTGGCGATTCGCCCGTTTTGTGTATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTGCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGtt                                                                                                   <  1:389879/250‑1 (MQ=255)                                                                                                                                ggtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGt                                                                                         <  1:301682/250‑1 (MQ=255)                                                                                                                                ggtggCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCACCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCCGCCTGACGTTGTGGGACaat                                                                                         >  1:257844/1‑248 (MQ=255)                                                                                                                                            aCCTGGTGCCTTGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTTTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGTGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTc                                                                               <  1:91481/248‑1 (MQ=255)                                                                                                                                                     cTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCt                                                                           <  1:281324/243‑1 (MQ=255)                                                                                                                                                     cTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGGGGTGCAGATCTTCCAGTCAGCtaagtcgg                                                                    >  2:281324/1‑243 (MQ=255)                                                                                                                                                                        gAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGt                                                <  1:70496/251‑1 (MQ=255)                                                                                                                                                                         aGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTaa                                                    >  2:192364/1‑246 (MQ=255)                                                                                                                                                                         aGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTaa                                                    <  1:192364/246‑1 (MQ=255)                                                                                                                                                                            cGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGGGCATTATGTTGGTAATCGTGCTg                                            >  2:321145/1‑251 (MQ=255)                                                                                                                                                                                              tCCCGCTGCTGGGCTTCTTTTGCACATTGCTTCCGGTGATGGCGATTTATCGTGGTCGTCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGTATTATGTTGGTTATCGTGCTTATATTTTTTCCCATtgt                           <  1:120686/250‑1 (MQ=255)                                                                                                                                                                                                 cGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTg                       <  1:154015/251‑1 (MQ=255)                                                                                                                                                                                                   ctgctgGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGCCGCTGAGCATTTTGTTGGTAATCGTGCTGATTTTTTTGCCCATTGTGTTGct                     >  2:265422/1‑251 (MQ=255)                                                                                                                                                                                                                tctGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCTCGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTACTGATATTTTTGCCCATTGTGTTGCTCTACActctct          <  1:119991/249‑1 (MQ=255)                                                                                                                                                                                                                   gCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAg      <  2:84019/250‑1 (MQ=255)                                                                                                                                                                                                                    cccATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGct    >  1:146746/1‑251 (MQ=255)                                                                                                                                                                                                                    cccATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGATGACGCTGCGCCTTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGAGTTGCTCTACACTCTCTGGAGct    >  2:172925/1‑251 (MQ=255)                                                                                                                                                                                                                      cATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGctac  <  1:51648/251‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    CCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTAC  >  NC_000913/1039906‑1040369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑