Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	953,552	C→G	M1M (ATG→ATC) †	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,552	0	C	G	100.0%	81.6 / NA	25	M1M (ATG→ATC) †	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (11/14);  total (11/14)

TGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCC  >  NC_000913/953314‑953772                                                                                                                                                                                                                                               |                                                                                                                                                                                                                             gggAAGCAACAGCGGTGACAAAGTCAGCTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTTCTTGGTCCCACAGGGGGGTGGTCTCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTTCGGAGTGTAGTTTTGCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGGCTCCTGTGGTAAAACCTTCACAGGATGTGGATAACTTTTCATGAAGCTCGGAGATGTacctccta                                                                                                                                                                                                                  <  2:362313/250‑8 (MQ=255)                 gtCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTACGTGGAtt                                                                                                                                                                                                  >  2:213284/1‑251 (MQ=255)                                cgcgTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGt                                                                                                                                                                                   >  1:207905/1‑251 (MQ=255)                                                           gCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGt                                                                                                                                                        >  1:122450/1‑251 (MQ=255)                                                           gCCTTACATTACTTTGTCCCACATGGTGGTGGTCGCGTCAGTAGCTCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTGTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGAGTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGt                                                                                                                                                        <  2:213833/251‑1 (MQ=255)                                                             cgtCCATTACTTGGACCCTCAGGGTGGTGGTCGCTGCAGTAGCGCCAGCCAGGAAGGACGCGGCCCCCTCGTACGGAGTGTAGTTTTTCTGAATGAATTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGTTCGtgt                                                                                                                                                      <  2:122450/249‑3 (MQ=255)                                                                       tttGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGAt                                                                                                                                                                                                   >  1:72369/1‑196 (MQ=255)                                                                       tttGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGAt                                                                                                                                                                                                   <  2:72369/196‑1 (MQ=255)                                                                        ttGTCCCACATGGTTGTGGTCGCTTCAGTAGCGCCAGCCAGTAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCCCCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGTAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGt                                                                                                                                            <  1:26044/250‑1 (MQ=255)                                                                          ggcccACATGGTGGTGGTCGCTTCGGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACTGACGTTTACTTCATTCTGCCAGTCACCTTTTGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCTGAGATGTAACACCTACCTTCTTAAGTGGATTTTGTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGTTaaa                                                                                                                                         <  2:359409/249‑1 (MQ=255)                                                                                    gtggtggtCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGt                                                                                                                                >  2:90041/1‑250 (MQ=255)                                                                                                           agccagGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAaccac                                                                                                        <  1:93240/251‑1 (MQ=255)                                                                                                            gccagGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGCCAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAaccacc                                                                                                       >  1:43550/1‑251 (MQ=255)                                                                                                              cagGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAaccaccac                                                                                                     <  1:19516/251‑1 (MQ=255)                                                                                                                      aCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGAAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATg                                                                                             >  1:222013/1‑251 (MQ=255)                                                                                                                                   gTACGGTGTGTAGGTTTTCTGAATTAAGTCACGGACGTTTCCTTCATTCGGCCAGTCACCTTTTGTAAATCCTTCCCAGGCTTTGGCTAACTTTTTATTAAGCTCGGAGATGTAACACCTACCTTCTTAGGTGGATGTTTTATTTACGGCGTACTTCGACAACCATTAATGGTGTGCGTGTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACTAACAAACCACCACCGATAATGTTGCCGATCGTaa                                                                                <  2:42019/251‑1 (MQ=255)                                                                                                                                                                       gTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACgg                                            <  1:213284/251‑1 (MQ=255)                                                                                                                                                                             ttCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGAGGTTTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCCCCACCAATAATGTTGCCGATCGTAACCGGAATAAGGTTATCAGTGATGAAATTCATCACGGTCaagg                                      >  2:117748/1‑248 (MQ=255)                                                                                                                                                                              tcaatCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAg                                        <  1:165676/244‑1 (MQ=255)                                                                                                                                                                                       cAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAAtt                            >  2:174261/1‑251 (MQ=255)                                                                                                                                                                                                         ccTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGAc          >  2:99744/1‑251 (MQ=255)                                                                                                                                                                                                            tCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACt         <  1:99744/249‑1 (MQ=255)                                                                                                                                                                                                                 ggCTGTGGCTAACTTTTCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCTCCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTcc  <  1:101031/251‑1 (MQ=255)                                                                                                                                                                                                                              ttttCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCg                                                                             >  2:204759/1‑163 (MQ=255)                                                                                                                                                                                                                              ttttCATTAAGCTCGGAGATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCg                                                                             <  1:204759/163‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                             TGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACCCAACCAACAAACCACCACCGATAATGTTGCCGATCGTAACCGGAATCAGGTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCC  >  NC_000913/953314‑953772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑