Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 77,502 C→T noncoding (136/227 nt)
R39C (CGC→TGC) 
sgrS →
sgrT →
small regulatory RNA SgrS
PtsG glucose transporter inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091377,5020CT93.3% 73.5 / NA 33noncoding (136/227 nt)
R39C (CGC→TGC) 
sgrS
sgrT
small regulatory RNA SgrS
PtsG glucose transporter inhibitor
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (15/13);  minor base G (0/2);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAG  >  NC_000913/77270‑77742
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
gAACTGTTGTTGCAGACGAGCAGATGTAATAAAAGGGGAACTCCGGTGCAAAAGACATCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGAGGAATCAAGGGGGAGCACCATGCGTCCGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGa                                                                                                                                                                                                                                  <  2:298933/250‑1 (MQ=255)
  aCTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACtt                                                                                                                                                                                                                               <  2:175752/251‑1 (MQ=255)
          ggtagccgagcaactagcagaaaaAGGGAACGCCAGTGCAAAAGACATCAATTTTATTTTGCCTATATTAAGTCAATAATTCCTAAAGATGAAGCAATGTGGTGCCCCATGCGTCAGTGTTTTCAGCACTATTTTTCCGCGCCAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGTAGGGCTGATTCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGcat                                                                                                                                                                                                                       <  2:80373/231‑1 (MQ=255)
           gCAGACGAGCAGATGTCAGAAAAGGGGAACTCCTGTGCAAAAGACAGTAATTTTATTTTTCCTATATTAAGGCAAGAATTCCTAACGATGAAGCAATGGTGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCAGGTTGCGTTGGTTAAGCGTCCCGCAACGATTAACCATGCTTACAGGGCTGATGCAGTGGGATGACTGCAATTCTGAAAGGTGACCTGCCTGcatc                                                                                                                                                                                                                      <  2:380286/251‑1 (MQ=255)
                 gAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTGTCCCTATAGTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCAtgtgt                                                                                                                                                                                                                <  2:320019/251‑1 (MQ=255)
                      gATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTg                                                                                                                                                                                                          <  2:100630/251‑1 (MQ=255)
                                  ggggAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTa                                                                                                                                                                                              <  1:136847/251‑1 (MQ=255)
                                                   aaGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCcagc                                                                                                                                                                               >  1:266089/1‑249 (MQ=255)
                                                         acAATTGTATGTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCACCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACACCGAATTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTTCAATTCTGAAAGTTGACTGGCCGGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATtata                                                                                                                                                                       <  1:28486/250‑1 (MQ=255)
                                                                             ttAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATtc                                                                                                                                                   >  1:395320/1‑251 (MQ=255)
                                                                                        atTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGCTTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCt                                                                                                                                         >  2:322228/1‑250 (MQ=255)
                                                                                              atcgagaaaGCAAGGGGGGGCCCCATGGTTCGTTTTTATCACCACTATTTTACCGCGACCGCGAATTTGTGCTGGTTGCGTGGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATAACTGCAATTCTAAAAGTGGACTTGCCTGCATAATGTGTGACTGAGTTTGGGTTTAAAACCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAgg                                                                                                                                  <  2:294555/244‑1 (MQ=255)
                                                                                              aaCGATGAAGCAAGGGGGTCCCCCATGCTTCTTTTTTATCAGCACTATTTTCCCGCGACAGCGAAGTTTTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGTTTAACCATGCTTGAAGGACTGATGCAGGGGGATGACTGCAATTCTGAAATTTGACTTGCCTGCATCATGTGTGACGGAGTATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCACGCCGCGCTaaaaa                                                                                                                                    <  1:180207/249‑1 (MQ=255)
                                                                                                cGATGAAGCAAGGGGATGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAggg                                                                                                                                 >  1:307153/1‑250 (MQ=255)
                                                                                                   tGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACg                                                                                                                             >  2:134883/1‑251 (MQ=255)
                                                                                                    caaGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGAACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGCCTTGCCTGCATCATGTGCGACTGAGCATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGACGCGCTAAACAGGGAACGt                                                                                                                            >  2:142854/2‑251 (MQ=255)
                                                                                                     aaGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGCGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGt                                                                                                                            >  2:86668/1‑250 (MQ=255)
                                                                                                     aaGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGt                                                                                                                            >  1:272557/1‑250 (MQ=255)
                                                                                                          aGGGGGTGCCCCAGGCGTCATTTTTATCAGCACTATTTTGCCGGGCCCGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACAATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAATTTGCCTTGCCTGCATCATGTTTGACTGAGTATGGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTGTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATc                                                                                                                      <  1:134883/251‑1 (MQ=255)
                                                                                                                 gCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATa                                                                                                                >  1:323596/1‑250 (MQ=255)
                                                                                                                          gTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATgg                                                                                                      >  1:62306/1‑251 (MQ=255)
                                                                                                                              gTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCg                                                                                                  >  1:139017/1‑251 (MQ=255)
                                                                                                                                  tagcagcctgattatACCGCGACAGCGAAGTTGTTATGGTTGCGTTGTTAAACGTGCCCACAGCGATTAACCATTCTTGAGGGACTGATGCAGTGGGAAGACGTAATGTCTGAAAGTTGCCTGGCTTGCATCATTGTTGACTGAGTATTGGTGTAAAAGCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATGCGCGCCGCGCTAAAAAGGGAACGTACTATCTGGATAATGACGATGGCTCGCCg                                                                                               <  2:62306/236‑1 (MQ=255)
                                                                                                                                                 aCCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTAc                                                                               >  1:163563/1‑251 (MQ=255)
                                                                                                                                                            gAAGTTGTGCTGGTGGCGTTGGTTAAGCGTCCCACACAGATTAACCATGCTGGAAGGACTGATGAAGGGGGATGACCGCAATTCTGAAAGTTGACTTGCCGGCATCATGTGTGACTGAGTATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTAt                                                                    <  2:139017/251‑1 (MQ=255)
                                                                                                                                                                        gTTGCGTTGGTTAAGCGTCCCCAAAAGATTACCCTGGCTGAAAGGACTGATGCAGTGGGATGATTGCAATTCTGAAAGTGGACTTGCCTGCATCTTTGGGGACTGAGTATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCttt                                                        <  2:163563/251‑1 (MQ=255)
                                                                                                                                                                              ttGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtt                                                                                                                                                             >  1:362776/1‑144 (MQ=255)
                                                                                                                                                                                  ttAAGCGTCCCAAAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTCTTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGAGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATgggg                                              >  2:412058/1‑251 (MQ=255)
                                                                                                                                                                                                      tAACCTTGCTTGAAGGACTGATGCGGTGGGATGACGGAAATTCGGAAAGTTGACTTGCCGCCATAATGTGTTACGGATTATTGGTGTAAAACCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAg                            <  2:323596/250‑1 (MQ=255)
                                                                                                                                                                                                      tAACCATGCTTGAAGTACTGATGCAGTGGGATGACTGCAATTCTGAAATTTGACTTCCCTGCATCATGTGTGATTGAGAATTGTTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCt                                                                                                                     <  2:333025/161‑1 (MQ=255)
                                                                                                                                                                                                      tAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCt                                                                                                                     >  1:333025/1‑161 (MQ=255)
                                                                                                                                                                                                                          aTGCAGTGGGATGACTGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTAttt       >  1:398668/1‑251 (MQ=255)
                                                                                                                                                                                                                               gTGGGATGACGGCAATTCTGAAGGTTGACTTGCCTGCAGAATTGGGGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAg  <  1:137573/251‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
GAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAG  >  NC_000913/77270‑77742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: