| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 77,502 | C→T | noncoding (136/227 nt) R39C (CGC→TGC) |
sgrS → sgrT → |
small regulatory RNA SgrS PtsG glucose transporter inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 77,502 | 0 | C | T | 93.3% | 73.5 / NA | 33 | noncoding (136/227 nt) R39C (CGC→TGC) | sgrS sgrT | small regulatory RNA SgrS PtsG glucose transporter inhibitor |
| Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (15/13); minor base G (0/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAG > NC_000913/77270‑77742 | gAACTGTTGTTGCAGACGAGCAGATGTAATAAAAGGGGAACTCCGGTGCAAAAGACATCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGAGGAATCAAGGGGGAGCACCATGCGTCCGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGa < 2:298933/250‑1 (MQ=255) aCTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACtt < 2:175752/251‑1 (MQ=255) ggtagccgagcaactagcagaaaaAGGGAACGCCAGTGCAAAAGACATCAATTTTATTTTGCCTATATTAAGTCAATAATTCCTAAAGATGAAGCAATGTGGTGCCCCATGCGTCAGTGTTTTCAGCACTATTTTTCCGCGCCAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGTAGGGCTGATTCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGcat < 2:80373/231‑1 (MQ=255) gCAGACGAGCAGATGTCAGAAAAGGGGAACTCCTGTGCAAAAGACAGTAATTTTATTTTTCCTATATTAAGGCAAGAATTCCTAACGATGAAGCAATGGTGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCAGGTTGCGTTGGTTAAGCGT‑CCCGCAACGATTAACCATGCTTACAGGGCTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGGTGACCTGCCTGcatc < 2:380286/251‑1 (MQ=255) gAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTGTCCCTATAGTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCAtgtgt < 2:320019/251‑1 (MQ=255) gATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTg < 2:100630/251‑1 (MQ=255) ggggAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTa < 1:136847/251‑1 (MQ=255) aaGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCcagc > 1:266089/1‑249 (MQ=255) acAATTGTATGTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCACCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACACCGAATTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTTCAAT‑TCTGAAAGTTGACTGGCCGGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATtata < 1:28486/250‑1 (MQ=255) ttAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATtc > 1:395320/1‑251 (MQ=255) atTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGCTTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCt > 2:322228/1‑250 (MQ=255) atcgagaaaGCAAGGGGGGGCCCCATGGTTCGTTTTTATCACCACTATTTTACCGCGACCGCGAATTTGTGCTGGTTGCGTGGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATAACTGCAAT‑TCTAAAAGTGGACTTGCCTGCATAATGTGT‑GACTGAGTTTGGGTTTAAAACCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAgg < 2:294555/244‑1 (MQ=255) aaCGATGAAGCAAGGGGGTCCCCCATGCTTCTTTTTTATCAGCACTATTTTCCCGCGACAGCGAAGTTTTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGTTTAACCATGCTTGAAGGACTGATGCAGGGGGATGACTGCAAT‑TCTGAAATTTGACTTGCCTGCATCATGTGT‑GACGGAGTATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCACGCCGCGCTaaaaa < 1:180207/249‑1 (MQ=255) cGATGAAGCAAGGGGATGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAggg > 1:307153/1‑250 (MQ=255) tGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACg > 2:134883/1‑251 (MQ=255) caaGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGAACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGCCTTGCCTGCATCATGTGC‑GACTGAGCATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGACGCGCTAAACAGGGAACGt > 2:142854/2‑251 (MQ=255) aaGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGCGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGt > 2:86668/1‑250 (MQ=255) aaGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGt > 1:272557/1‑250 (MQ=255) aGGGGGTGCCCCAGGCGTCATTTTTATCAGCACTATTTTGCCGGGCCCGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACAATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAATTTGCCTTGCCTGCATCATGTTT‑GACTGAGTATGGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTGTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATc < 1:134883/251‑1 (MQ=255) gCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATa > 1:323596/1‑250 (MQ=255) gTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATgg > 1:62306/1‑251 (MQ=255) gTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCg > 1:139017/1‑251 (MQ=255) tagcagcctgattatACCGCGACAGCGAAGTTGTTATGGTTGCGTTG‑TTAAACGTGCCCACAGCGATTAACCATTCTTGAGGGACTGATGCAGTGGGAAGAC‑GTAATGTCTGAAAGTTGCCTGGCTTGCATCAT‑TGTTGACTGAGTATTGGTGTAAAAGCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATGCGCGCCGCGCTAAAAAGGGAACGTACTATCTGGATAATGACGATGGCTCGCCg < 2:62306/236‑1 (MQ=255) aCCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTAc > 1:163563/1‑251 (MQ=255) gAAGTTGTGCTGGTGGCGTTGGTTAAGCGT‑CCCACACAGATTAACCATGCTGGAAGGACTGATGAAGGGGGATGACCGCAAT‑TCTGAAAGTTGACTTGCCGGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTAt < 2:139017/251‑1 (MQ=255) gTTGCGTTGGTTAAGCGT‑CCCCAAAAGATTACCCTGGCTGAAAGGACTGATGCAGTGGGATGATTGCAAT‑TCTGAAAGTGGACTTGCCTGCATCTTTGGG‑GACTGAGTATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCttt < 2:163563/251‑1 (MQ=255) ttGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtt > 1:362776/1‑144 (MQ=255) ttAAGCGT‑CCCAAAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTCTTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGAGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATgggg > 2:412058/1‑251 (MQ=255) tAACCTTGCTTGAAGGACTGATGCGGTGGGATGACGGAAAT‑TCGGAAAGTTGACTTGCCGCCATAATGTGT‑TACGGATTATTGGTGTAAAACCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAg < 2:323596/250‑1 (MQ=255) tAACCATGCTTGAAGTACTGATGCAGTGGGATGACTGCAAT‑TCTGAAATTTGACTTCCCTGCATCATGTGT‑GATTGAGAATTGTTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCt < 2:333025/161‑1 (MQ=255) tAACCATGCTTGAAGGACTGATGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCt > 1:333025/1‑161 (MQ=255) aTGCAGTGGGATGACTGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTAttt > 1:398668/1‑251 (MQ=255) gTGGGATGACGGCAAT‑TCTGAAGGTTGACTTGCCTGCAGAATTGGG‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAg < 1:137573/251‑1 (MQ=255) | GAACTGTTGTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGT‑CCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAAT‑TCTGAAAGTTGACTTGCCTGCATCATGTGT‑GACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAG > NC_000913/77270‑77742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |