Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 690,948 G→A V279V (GTC→GTT)  ybeX ← CorC‑HlyC family protein YbeX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913690,9480GA96.2% 165.7 / ‑1.1 54V279V (GTC→GTT) ybeXCorC‑HlyC family protein YbeX
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (26/25);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

GCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATC  >  NC_000913/690709‑691187
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               
gCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTg                                                                                                                                                                                                                                      <  1:23424/251‑1 (MQ=255)
  ggCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAACGCCATGTAGTTATCTATCCCGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGa                                                                                                                                                                                                                                     >  1:379096/1‑250 (MQ=255)
              tGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGGAAGGAGAGAAGGCCAGCGTTCCGCAGGGACCGGATAATAACGCCAGCAGCCGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCCACCGGGATTTTAACATGGACCTGAATAATACCCCgcc                                                                                                                                                                                                                        >  2:119887/1‑249 (MQ=255)
                        ttAAAGGACAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGAAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGAAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAAAACGCCGACTGTCGGACAt                                                                                                                                                                                                              >  2:371349/1‑251 (MQ=255)
                          aaaGGTCAACGCCTGAATCCCCATCAGCGAAAGAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCCCCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGTGTGAGTCATCCGGTATTTTAACATGAACCTGAATAATACGCCGTCTTTCGGCCATc                                                                                                                                                                                                             <  2:128164/250‑1 (MQ=255)
                            aGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGcc                                                                                                                                                                                                          >  1:325941/1‑251 (MQ=255)
                                      cTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACt                                                                                                                                                                                                >  2:385804/1‑251 (MQ=255)
                                        gAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTg                                                                                                                                                                                               <  1:385804/250‑1 (MQ=255)
                                          aGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGaataat                                                                                                                                                                                                                                >  2:209477/1‑215 (MQ=255)
                                          aGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGaataat                                                                                                                                                                                                                                <  1:209477/215‑1 (MQ=255)
                                                aTCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCgtc                                                                                                                                                                                      >  1:131827/1‑251 (MQ=255)
                                                  cAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCgtcg                                                                                                                                                                                     <  1:309589/250‑1 (MQ=255)
                                                               cccgtaggcaataCGTCTTAAGCAGAGAAGGCCAGCATTTCGCCGGCACCGTATAATTACGCCAGCATCGGGATAATGCGATGTATGTCAATTAATGAGGCTATAGTGATGTAGCTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTATCATGATCCTGAATAAGTCGCCGACTGTCGGCCATCGCCATTTTGAACTGTTAATCGTCTATGTCGATAGTTTc                                                                                                                                                                       <  2:144857/239‑1 (MQ=255)
                                                                        cAGACGTCGTCTGGAGCGCAAGCCAGCGTTCCGCAGGCCCCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCCATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTAGGTTTTCTTCATCCAGCTTCGGCTGGGGTGAGACATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGCTGTCGAAAGTTTCGCCACgcgc                                                                                                                                                              >  2:252227/1‑251 (MQ=255)
                                                                         aGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGACCCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGACAACCGTAGATGTCGATAGTTTCGCCACGCGcc                                                                                                                                                             >  1:378439/1‑251 (MQ=255)
                                                                          gACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGATTTATTCATCCAGCTTCGGCTGTGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTAGATAGTTTCGCCACGCGCCg                                                                                                                                                            >  1:85607/1‑251 (MQ=255)
                                                                                  aaGGAGAGAAGGCCAGCGTTCCGCAAGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGATTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGATAAACGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGc                                                                                                                                                    >  1:337774/1‑251 (MQ=255)
                                                                                    ggAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGccc                                                                                                                                                  >  1:305954/1‑251 (MQ=255)
                                                                                                      ccGCTGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTAGTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTGTAACATGAACCTGAATAATACGCCGACGGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGAGGCCCAAATGCCTGCATCACCa                                                                                                                                 <  2:379096/250‑1 (MQ=255)
                                                                                                         ccggCACCGAAAAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTAGCCAGTCTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAgccc                                                                                                                             <  2:313173/249‑4 (MQ=255)
                                                                                                                 gAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGAt                                                                                                                       >  1:397561/1‑249 (MQ=255)
                                                                                                                  aataatAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAgt                                                                                                                    <  2:397561/251‑1 (MQ=255)
                                                                                                                   ataataACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAgtg                                                                                                                   <  2:192676/251‑1 (MQ=255)
                                                                                                                         aCGCCAGCAGCAGGCGAATGCGCTGGCGTGCAATTAATGAGGCAAAAGCCAGGTAGTTATCTTGCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCTGGATGTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGAct                                                                                                             <  2:261654/251‑1 (MQ=255)
                                                                                                                          cGCCAGCATCAGGCGGAAGCGCTGGCGTTCAATTAATGAGGCAAAAGCCCTTGAGTTATCTATCCAGTTTCTGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCCTCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGActt                                                                                                            <  2:91051/251‑1 (MQ=255)
                                                                                                                           gCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGActtc                                                                                                           >  1:19068/1‑251 (MQ=255)
                                                                                                                              agcagcagGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCCCCAGACCACCGATAGTGTCGACttcttc                                                                                                        >  2:411451/1‑251 (MQ=255)
                                                                                                                              agcagcagGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACttcttc                                                                                                        <  1:181811/251‑1 (MQ=255)
                                                                                                                                 agcagGCGAATGCGCTGGCGTGCAATTAATGAGGCAAAAGCCATGTAGGTATCTATCCAGTTTCTGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACATGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTATCCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATc                                                                                                     <  1:375539/251‑1 (MQ=255)
                                                                                                                                      gCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTaa                                                                                                <  1:161802/251‑1 (MQ=255)
                                                                                                                                                             aTGAGGCAAAAGCCATGTAGATATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTTACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCCAATGCCTGCATCCCCAGACCACCGCTAGTGTCGCATTCTTCATCGACAAAGTGGGGAGCGAAAGCTTCGtt                                                                          >  2:364269/1‑250 (MQ=255)
                                                                                                                                                                 ggCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGt                                                                     >  1:128594/1‑251 (MQ=255)
                                                                                                                                                                   cAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAATTGGGTGCCGAACGCTTCGTTGAAgact                                                                   >  2:281491/1‑248 (MQ=255)
                                                                                                                                                                   cAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCt                                                                   <  1:59655/251‑1 (MQ=255)
                                                                                                                                                                      aagcctagaagttagttatcCAGTTTCGGTTTTAATCATCCAGCTTCGGCTGGGGTTAGTCAGCCTGGATTTTAACATGAACCTGAAGAATACGCCGACTGTCGGCCATCGCCACTTTGAACTTGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTGCTTCGGCGCTAAAGTGGGTGCCGAACGCGTCGTTGAAGTcggca                                                                <  2:256794/235‑5 (MQ=255)
                                                                                                                                                                                 ttctctatcCCGTTTCGGTTTTATTCATCCAGCTTCGGCTGTGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTACACTGGTAACCGTAGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCAAAATGCCTGAATCACCGGACCACCGCTAGTGGCGACTTCTTCATCACTGTAGTTGCTGCCGACCGCATCGATGATGTCTTCCATGGGAGcc                                                       >  2:113441/4‑249 (MQ=255)
                                                                                                                                                                                     ctatcCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACt                                                                                                                                                                                                       <  1:335865/101‑1 (MQ=255)
                                                                                                                                                                                     ctatcCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACt                                                                                                                                                                                                       >  2:335865/1‑101 (MQ=255)
                                                                                                                                                                                      tatcCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTgcgc                                                >  1:9541/1‑251 (MQ=255)
                                                                                                                                                                                        tcCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACTCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTc                                                                                                                                                                       <  2:316867/130‑1 (MQ=255)
                                                                                                                                                                                        tcCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCCTCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTc                                                                                                                                                                       >  1:316867/1‑130 (MQ=255)
                                                                                                                                                                                         ccAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGt                                              >  1:158450/1‑250 (MQ=255)
                                                                                                                                                                                         ccAGTTTCGGTTTTATTCATCCAGCTCCGGCTGGGGTGAGTCATCCGGGATTTTAACAGGATCCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCGCCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGt                                              <  2:92549/250‑1 (MQ=255)
                                                                                                                                                                                          ccctccccgccgctcttcctgtctttCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTTTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGTAAGCCAGTGCGCGTAc                                            <  2:234512/227‑1 (MQ=255)
                                                                                                                                                                                            gTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACgg                                          >  1:391423/1‑251 (MQ=255)
                                                                                                                                                                                                cGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACgtcca                                       <  2:158450/250‑5 (MQ=255)
                                                                                                                                                                                                           aTCCAGCTTCGGCTGGGGTGAGACATCCGGGCTTTTAACATGAACCTGCATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATCGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGCCCACCGATAGTGTCGCCTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGGAGCCCGTGCGCGTACGGTCCAGGTTTgacga                            >  2:274643/1‑250 (MQ=255)
                                                                                                                                                                                                                  ttCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGGGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTAc                                            >  1:234512/1‑227 (MQ=255)
                                                                                                                                                                                                                     ggctttggggAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGGCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGAc                  <  2:384289/241‑1 (MQ=255)
                                                                                                                                                                                                                        gggggggAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGTAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGaa              <  2:325941/250‑1 (MQ=255)
                                                                                                                                                                                                                              gAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGat        <  1:90181/251‑1 (MQ=255)
                                                                                                                                                                                                                                gTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGatat      >  1:57391/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aTCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATatcat   >  1:131485/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ccGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATatcatc  <  1:183852/250‑1 (MQ=255)
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GCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATC  >  NC_000913/690709‑691187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑