Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	94.9%	128.2 / ‑1.7	39	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base C (18/19);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTC  >  NC_000913/4636687‑4637168                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    gggCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCgcgc                                                                                                                                                                                                                                         >  1:207238/1‑251 (MQ=255)     cTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGttt                                                                                                                                                                                                                                              <  1:89256/242‑1 (MQ=255)                  cGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCAtt                                                                                                                                                                                                                         <  1:199075/250‑1 (MQ=255)                     cATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACa                                                                                                                                                                                                                     <  1:267952/251‑1 (MQ=255)                      aTGCGGTTGTTGCTGGGTGATTTTTTACTGGTGGCGGTGGCAGCCTTGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCGGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACaa                                                                                                                                                                                                                    <  1:43793/251‑1 (MQ=255)                          ggTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCGTGGTTCGAACTGGCCATTTTTGTCAAAGAAGTTCAACCGGGCGGGCGCAACGCAACGGAGGGGACGATGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGTTATCTGGGGACCCAACGCCGGGGCACCTGGCGCACGCGTTTAATCAGCTACACCATCCCCCGGTTCGCGACACTATCGGTgtccttaaaaaaagg                                                                                                                                                                                                                >  1:136668/1‑237 (MQ=255)                                tgCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATAGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGGTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCAATAAAAAAGtccgaaat                                                                                                                                                                                                          >  1:192778/1‑244 (MQ=255)                                   ggggCTATTGTTTACTGTTGGCGGTGTCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAATAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGaa                                                                                                                                                                                                       <  2:341333/250‑1 (MQ=255)                                    gggCTATTGTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGaa                                                                                                                                                                                                       <  1:67473/250‑1 (MQ=255)                                        ttttttttACTGGTGGCGGTGGCAGCCTTGTTCTGACGGGCCATTGTTGTCAAAGAAGTTCAACCTGGCGTGCGAAGAGCAACGGAGGGGACGTGGATCGACACCGCAACGTTGCTGGCGGAGCGGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTTGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTGCGTGCCAATATCGGTGGCAATAACAAACTGCGCAAGGAatagc                                                                                                                                                                                                   <  2:233038/248‑3 (MQ=255)                                          ttttttACTGGGGGCGGTGGCCGCCTTGTTCGTACTGGCCATTTTTGGCAAAGAAGTTAATCCGGGCGTTCGAAGAGCAACGGAGGGAACGTTGATCGACACCGCAACGTTGCTGGCGGGGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCAt                                                                                                                                                                                                 <  2:315666/250‑1 (MQ=255)                                             tttACTGGTGTCGTTGACAGCCTGTTTCGTACTGGACATTTGTGTCAAAGAAGTTAAACCAGGCGTGCGACGAGAAACGGAGGGGTCGTTGATCGACCCCGCACCGTTGCCGGCGGATCTGCCGCGACCCGCTTTTCTCTCTGGTGCCCCACCGCATGGTCATCTGGCGCAGGCGTTTACTCATCTTCAATAACCCCCGTTTCGCGTCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGt                                                                                                                                                                                               <  2:263502/249‑1 (MQ=255)                                                   ggtggcggtggcATCCGGGTTCGTACTGGCCAGTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTatat                                                                                                                                                                                         <  2:346473/249‑1 (MQ=255)                                                                gCCTGGGTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGGTGCCGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGAGGCGCAggg                                                                                                                                                                          <  2:415832/251‑1 (MQ=255)                                                                        cgttcTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTAt                                                                                                                                                                  <  1:310594/247‑1 (MQ=255)                                                                                     tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCgg                                                                                                                                                      <  2:207238/250‑1 (MQ=255)                                                                                                   tAAACCTGGCGTGCGAAGCGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCCACTGGCGCAGGCGTCTAATCAGCTACCAAATCCCCCGGTTCGCGCCACTCTCGGTGGCATTAACTAAGTGCGCAATGAATATCCTGTCAATATGACTGCTGCGCAGGGAATATAATTTGTCGATTCTGCAAATAAAGCAGTTg                                                                                                                                       >  2:154473/1‑251 (MQ=255)                                                                                                         gggCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAgg                                                                                                                                 >  1:39293/1‑251 (MQ=255)                                                                                                             gTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAggtttt                                                                                                                             >  1:195858/1‑247 (MQ=255)                                                                                                                 gaagaaCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAACTGGCGCGTCCCGATTTGCTCTCTGGGGAACCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACCACATCCCCCGTTTCGCGCCAATATCGGAGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGAAGGGCCAAGAATTGTTCGATTCGGCAAATAAAACCGTTGGAGAGGATTATTcg                                                                                                                         >  2:272258/1‑251 (MQ=255)                                                                                                                    gagCAACGGCGGGGACGTTGATCGACACCTCCACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCACAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGCAAAGTATTGTCAGATTAGGCAACTAAAGCCGTTGGACAGGattattaacgc                                                                                                                      >  2:299707/1‑246 (MQ=255)                                                                                                                                       gATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTa                                                                                                    >  1:115257/1‑250 (MQ=255)                                                                                                                                       gATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTACCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTaa                                                                                                   <  1:128644/251‑1 (MQ=255)                                                                                                                                           gACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGtt                                                                                               >  1:50680/1‑251 (MQ=255)                                                                                                                                                  cAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCCACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAACGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGt                                                                                        >  1:183771/1‑251 (MQ=255)                                                                                                                                                    atggtGCTGGCGGAGCTGGCGCATCCCGATTTTCTCGCGGGGGACCCAACGCATGGGCAACTGGCGCATGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCGCAATGAATAGCATGTCGATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTGTGACAGGATTAGTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCa                                                                                      <  2:136668/247‑1 (MQ=255)                                                                                                                                                         gCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCAtt                                                                                                                                                                                                                         <  1:243070/115‑1 (MQ=255)                                                                                                                                                         gCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGGGGCAtt                                                                                                                                                                                                                         >  2:243070/1‑115 (MQ=255)                                                                                                                                                                gAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCg                                                                                                                                                       >  2:281621/1‑174 (MQ=255)                                                                                                                                                                gAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCg                                                                                                                                                       <  1:281621/174‑1 (MQ=255)                                                                                                                                                                                          tGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATc                                                >  2:42266/1‑251 (MQ=255)                                                                                                                                                                                                      gCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCAGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGGTCtg                                    >  2:328171/1‑251 (MQ=255)                                                                                                                                                                                                        aTGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCtgtg                                  <  1:153820/251‑1 (MQ=255)                                                                                                                                                                                                              aaCTGGCGCAGGAGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATTCGCAGGGTAAAGTATTGATCGATTCGGCAAATAAAGCCGTTGGGCAGGATTATTCGCGCTGGAATGACGTCTGGCGAACGTTGCGTGGTCAGAATGGTGCACGCAGCACGTTGCAAAATCCTGCCGATCCCGACAGTTCGGTGatgtat                            >  2:184160/1‑251 (MQ=255)                                                                                                                                                                                                                  ggCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCCATGATTATCATGTCTATATGCCCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTAGGGTGCGGGCAGCAGGTTGCAAAATCCTGCCGATCCCGACAGTTCTGGGATGTATGTTg                        >  2:49051/1‑251 (MQ=255)                                                                                                                                                                                                                             ttAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAATCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGAt              <  1:408963/250‑1 (MQ=255)                                                                                                                                                                                                                             ttAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCgctt             >  2:300306/1‑248 (MQ=255)                                                                                                                                                                                                                                    gCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACg      <  1:300306/251‑1 (MQ=255)                                                                                                                                                                                                                                        caacaTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTc  >  1:115911/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    GGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTC  >  NC_000913/4636687‑4637168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑