Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,238	T→G	intergenic (+445/+452)	insO → / ← fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,238	0	T	G	100.0%	86.4 / NA	28	intergenic (+445/+452)	insO/fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/14);  total (14/14)

TTCGCGCTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAAT  >  NC_000913/4509994‑4510473                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            ttCGCGCTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGCCTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGc                                                                                                                                                                                                                                       >  1:192582/1‑251 (MQ=255)        tttCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGCTCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCAAACTTTTTAAAAAAATCGATGGTCAGAAACTGGATTAGCACTTCCGTTCCAGGGTTGCTTATGAt                                                                                                                                                                                                                                >  2:334632/1‑251 (MQ=255)                                         ccGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCCCACTTTTTCGAAACATCGATGGTCAGAAACTGGATTCGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTc                                                                                                                                                                                                >  2:184112/1‑250 (MQ=255)                                                        ttATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAGGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATGACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCAt                                                                                                                                                                                >  2:83547/1‑251 (MQ=255)                                                            gCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAGGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATGACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGt                                                                                                                                                                            <  1:83547/251‑1 (MQ=255)                                                                                       ccGACTCCTGTATATATCCTTGATAACAAGCGATCCATCAACTGGCCTTTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACATAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCTTCTGATCATTTATTTATCCTCAAAAGCCCAACCTCATTGGGAATGAACCATCTTCGTGAATGTCCGct                                                                                                                                                 <  1:279252/251‑1 (MQ=255)                                                                                       ccGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGTACCAGCTCCGTGAATGTCCGct                                                                                                                                                 >  2:246488/1‑251 (MQ=255)                                                                                          aCTCATGAAGATATCATTGATAACAATCGAACCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGGCTGAACATTGATTTATCCCCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTg                                                                                                                                              <  2:192582/251‑1 (MQ=255)                                                                                             cATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGc                                                                                                                                            <  1:184112/250‑1 (MQ=255)                                                                                               ggAAGATATCATTGATAACAATCGAACCATCAACTGGCCTGTATAACTTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTAGCCTCAAACGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGcaca                                                                                                                                         <  2:108181/250‑1 (MQ=255)                                                                                                gAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCAGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGACTGATCATTGATTTATCCTCAAAAGCCCAACCTAATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGcac                                                                                                                                          >  1:268779/1‑249 (MQ=255)                                                                                                       ccATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCTGCGCTGGCACAGAGCGaaa                                                                                                                                 <  2:23618/250‑1 (MQ=255)                                                                                                               aaCAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCTCTCTGGCACAGAGCGAAATTTTTTg                                                                                                                          >  1:318108/1‑250 (MQ=255)                                                                                                                  aaTCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGAtct                                                                                                                      >  1:236126/1‑251 (MQ=255)                                                                                                                         ccATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCAt                                                                                                                                                                                >  2:254191/1‑186 (MQ=255)                                                                                                                         ccATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCAt                                                                                                                                                                                <  1:254191/186‑1 (MQ=255)                                                                                                                          cATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTg                                                                                                              <  1:152795/251‑1 (MQ=255)                                                                                                                               aCTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCTCACTTTTTATAAAAATCGATGTTCAGAAACTGGATTAGCAATTCCGTGCCAGGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCGCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATc                                                                                                         <  1:401191/251‑1 (MQ=255)                                                                                                                                     cTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACt                                                                                                   >  2:381780/1‑251 (MQ=255)                                                                                                                                       gTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTa                                                                                                 <  1:324181/251‑1 (MQ=255)                                                                                                                                            aCGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCAt                                                                                            <  1:360703/251‑1 (MQ=255)                                                                                                                                                          gATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGa                                                                                                                         <  2:93759/208‑1 (MQ=255)                                                                                                                                                          gATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGa                                                                                                                         >  1:93759/1‑208 (MQ=255)                                                                                                                                                                gAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTa                                                                        <  2:101954/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttAGCAATTCCGTTACAGGGTTGATTGTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCCAAAGCAAAAACTCATTGGTAATGAAAAAGCTCCGTGAATGTCAGCTCTGGCCCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTCAACTTAGACATGTCACGTTTTTGGGTTTCTAAACTTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCaa     >  2:274027/1‑250 (MQ=255)                                                                                                                                                                                                                                      aGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAat  >  1:39376/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttCCAGGGTTGCTTTTGCTTTACGTTGGCGTCTGATCATTGATTTATCCTCCAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAc                                                                                       >  2:60485/1‑156 (MQ=255)                                                                                                                                                                                                                                                ttCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTTCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAc                                                                                       <  1:60485/156‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            TTCGCGCTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAAT  >  NC_000913/4509994‑4510473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑