Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,688	C→A	H57N (CAC→AAC)	priB →	primosomal replication protein N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,688	0	C	A	100.0%	149.7 / NA	48	H57N (CAC→AAC)	priB	primosomal replication protein N
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/25);  total (23/25)

CGCCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAC  >  NC_000913/4425442‑4425934                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        cgcCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGACCGTTATTGTTAGCGGAAACg                                                                                                                                                                                                                                                      >  1:217597/1‑250 (MQ=255)    cGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGaaa                                                                                                                                                                                                                                                   >  1:20500/1‑250 (MQ=255)    cGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGaaa                                                                                                                                                                                                                                                   >  1:234473/1‑250 (MQ=255)          cGTCGCGTTGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTTTCAAATGCCCGGTATTGTTAGCGGAAACGAAAAACAGGc                                                                                                                                                                                                                                            >  2:37201/1‑251 (MQ=255)           gTCGCGATGATTTCGCAAACGACACCGATGATGATGCTGACGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCCCCGTGTGCCGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGCGAATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGCCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGCAAAACAGGcc                                                                                                                                                                                                                                           >  1:349959/1‑251 (MQ=255)             cgcgATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCa                                                                                                                                                                                                                                          <  1:73688/250‑1 (MQ=255)                   gatgtCGCCAACGAAACCGCTGATGATGCTGAAGCTGGGGATGCTGAAGAGTAATTTCTGATGACCAACCGTCGGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCATTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTAcgca                                                                                                                                                                                                                                   <  2:130437/247‑4 (MQ=255)                        cGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGt                                                                                                                                                                                                                               >  1:111358/1‑250 (MQ=255)                            aaCGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGACTTCCTCACTGCCCGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGGGTCAAATGCCCGTTATTGGTAGCGGAAACGAAAACCAAGCCATTACTCACAGGATaa                                                                                                                                                                                                                           >  2:404360/1‑250 (MQ=255)                                  aCCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCgg                                                                                                                                                                                                                    <  2:417292/251‑1 (MQ=255)                                           gatgCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCAt                                                                                                                                                                                                           <  1:358360/251‑1 (MQ=255)                                                          gATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTTCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCTGCAGTCGCATAACCGTTCAGGGGtt                                                                                                                                                                                            <  2:75312/251‑1 (MQ=255)                                                                   gagTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATg                                                                                                                                                                                  <  1:279868/251‑1 (MQ=255)                                                                         tttCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAAAGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGcccca                                                                                                                                                                             >  2:195514/1‑247 (MQ=255)                                                                           tCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGGTATTGTTAGCGGAAACGAAAAACAGGCCATTAATAACAGTATAACGGTCGGCAGTCACATAACCGTTCAGGGGTT‑CATTTCATGcctaaagg                                                                                                                                                                          >  1:218308/1‑245 (MQ=255)                                                                              gatgaCCAACCGCTTGGTGTGGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCTCCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCaa                                                                                                                                                                       <  2:258398/251‑1 (MQ=255)                                                                               atgaCCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGACGGCAGTCGCATAACCGTTCCGGGGTT‑CATTTCATGCCACAAGGcca                                                                                                                                                                       >  2:2847/1‑248 (MQ=255)                                                                                  cctcaccgccgggTGTTGTCCGGCACTGTGGGCAGGGCCCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGGCAGTTCGTGGTTGAGCATCGTTTTGTGCAGGAGGAAGCCGTATTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGAGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGCCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCgaagaa                                                                                                                                                                   <  2:250492/240‑1 (MQ=255)                                                                                        cGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAAACGTTCAGGGGTT‑CATTTCATGCCACACAGCAAAGAACGGACt                                                                                                                                                             >  2:91063/1‑251 (MQ=255)                                                                                                gttgtCCGGCACCGTGTGCAGGGCTCCCCTGCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGACAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGGTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCaaa                                                                                                                                                      <  2:333697/250‑1 (MQ=255)                                                                                                        gCACCGTGTGCAGGGCTCCCCTTCGAACAGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTCTTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGACGCATAACCGTTCAGGGGTTACATT‑CCTGCCACAAGGCCACGAACGGACTGAgacaaatacatcgg                                                                                                                                             >  2:207292/1‑238 (MQ=255)                                                                                                        gCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGATTTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAAt                                                                                                                                                    <  1:207292/244‑1 (MQ=255)                                                                                                                           ccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGGCAAATGCCCGTTATTGTTAGCGGAAACGCAAAACAGGCCCTTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGTGCAAAATGGTTTTGCATTCCGAGCAGataaaat                                                                                                                          >  2:47480/1‑246 (MQ=255)                                                                                                                                 aaaGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAg                                                                                                                    <  2:111358/251‑1 (MQ=255)                                                                                                                                  aaGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGAt                                                                                                                      >  1:104090/1‑248 (MQ=255)                                                                                                                                      tCAGTCCATCAGGAATTCCTCACTGCCAGTTAGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCt                                                                                                               >  1:107128/1‑251 (MQ=255)                                                                                                                                             aTCAGGATTTCCACCCTGCCATTTCGTGCTTGCGCATCGTTCGGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGGGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACGCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACt                                                                                                        <  2:206593/251‑1 (MQ=255)                                                                                                                                                       ccTCCCTGCCAGTTCGTGCTGGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATgg                                                                                              <  1:342090/251‑1 (MQ=255)                                                                                                                                                       ccTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGTTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATg                                                                                               <  1:28978/250‑1 (MQ=255)                                                                                                                                                           aCTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCaaa                                                                                                                                                                      >  2:197970/1‑175 (MQ=255)                                                                                                                                                                 aGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCCCACGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCCCGtttttt                                                                                    >  2:63593/1‑251 (MQ=255)                                                                                                                                                                 aGTTCGTGCTTGAGCATCGTTCTGGGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTAttt                                                                                    <  2:22060/251‑1 (MQ=255)                                                                                                                                                                                aTCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGGTAGATTCTGGAGACTAGCCATATGGCACGGTATTTCCGtcgtcgacattt                                                                     >  2:371766/1‑245 (MQ=255)                                                                                                                                                                                aTCGTTCGGTGCAGGTGGAAGCCGGGTTTAACCGGCAGGCGTGGTGTCAAAGGCCCGTTATTGTGAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAGCCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGtt                                                                     <  2:147368/251‑1 (MQ=255)                                                                                                                                                                                 tctttCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCgtcgtc                                                                            <  2:366953/240‑1 (MQ=255)                                                                                                                                                                                 tCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCgtcgtc                                                                            >  1:366953/1‑243 (MQ=255)                                                                                                                                                                                        gtgCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGtt                                                             <  1:195514/251‑1 (MQ=255)                                                                                                                                                                                        gggCAGGAGGAAGCCGGCTTCCACCGGCAGGCGTGGTGGCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCTTCGTCGCAAGTTCTGCCGt                                                              <  2:38556/248‑1 (MQ=255)                                                                                                                                                                                                     ccGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAgg                                                <  1:91063/251‑1 (MQ=255)                                                                                                                                                                                                      cGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGCCTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCCCCGCGGAAGGc                                               >  2:396179/1‑251 (MQ=255)                                                                                                                                                                                                          tttCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTACCGGAAACGAAAAACAGGCCATTACTCACCGTATAACGGTCGGCAGTCGCATAACCGGTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGCGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTc                                           >  2:416805/1‑251 (MQ=255)                                                                                                                                                                                                                             gtCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGGTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCAGATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGa                        <  1:37201/251‑1 (MQ=255)                                                                                                                                                                                                                               cAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGata                      <  1:396179/251‑1 (MQ=255)                                                                                                                                                                                                                                  atgaccGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCg                   <  1:348323/247‑1 (MQ=255)                                                                                                                                                                                                                                    gccctctATTTTTAGCGGAAACGAAAAACAGGCCATTTCTCACAGTATAACGGCCTGCATTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGGCGCAATTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGAGCGACTATAAAGATATCGCt                 <  1:365291/245‑1 (MQ=255)                                                                                                                                                                                                                                        gTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCTGTCGTCGCAAGTTCTGCCGTTTACCCGCGGCATGCGTTCAAGAGATCGACTATAAAGATATctctccgc             >  2:13356/1‑244 (MQ=255)                                                                                                                                                                                                                                        gTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCACGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGCGATCGACTATAAAGCTATCGCTACGc             >  2:202748/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAc  <  1:54756/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        CGCCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTT‑CATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAC  >  NC_000913/4425442‑4425934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑