Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,347	T→A	intergenic (+138/‑131)	metA → / → aceB	homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,347	0	T	A	94.1%	45.2 / NA	17	intergenic (+138/‑131)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (7/9);  minor base C (1/0);  total (8/9)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGT  >  NC_000913/4215107‑4215587                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 cTGGCATAGTCAAGGTAATTTACTGTTTACCAACTGACTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAAAGCTGGATTAATATTCTGTGATATGCACTTGATACACGAGTCCGCACCTTACCTCAGGCACCTTCGGGGTCCTTTTTTATTTCCGAAACGGCCCTCAGCAGGTGAATAACTTTTATTCATATTTGTT‑TCAACACATTATCAAGTATTTCTACTTAAAAt                                                                                                                                                                                                                                        >  2:109087/1‑251 (MQ=255)       tAGTCACGGTAATTTACTGTTTACCAACTGGCGCAACTATTACGTCTACCAGATCACGCCAGACGATCTACGGCACATGAATCCAACGCTGGATTAATCGGCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATACATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGaaa                                                                                                                                                                                                                                   <  2:379550/250‑1 (MQ=255)              ggTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACTATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGttttt                                                                                                                                                                                                                           <  2:226546/251‑1 (MQ=255)                                  cTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCAAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGAACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATg                                                                                                                                                                                                         >  1:252803/1‑249 (MQ=255)                                                         aGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGaa                                                                                                                                                                                <  1:266297/251‑1 (MQ=255)                                                                         tCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATAGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCCGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGaacc                                                                                                                                                                >  2:17495/1‑248 (MQ=255)                                                                            aCGGCACATGAATCCAACGCTTGATTAATCTTCTGTGATAGTCTATCGTTAAGCGATACAGTACCTTACCTCAGGCACCTTCGTTTGCCTTTTTTATTTCCGAAACGGACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTGTGATTTTGCATTTTAAATGAGTAGTCTTATTTGTGCTGAACGAAAAGATCACAACg                                                                                                                                                                    <  2:259059/244‑1 (MQ=255)                                                                            aCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATc                                                                                                                                                                 <  2:289016/247‑1 (MQ=255)                                                                                                                                     tCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGGGGGGAAGTTTTCGGATCCATGACGAGGATCTGCACGATGACTGAACa                                                                                                     <  1:104112/250‑1 (MQ=255)                                                                                                                                       atcACCTTCCCTCAGGCACTTTCGGGTGCCGTATTTATTGCCGAAACTTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTGGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTGCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATTACTGAACAGGc                                                                                                  <  2:227774/249‑1 (MQ=255)                                                                                                                                                   cAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAAcc                                                                                        <  2:54086/249‑1 (MQ=255)                                                                                                                                                                                                                ttCATATT‑GATATCAACAAGTTATCAAGTATTTATAATTAATATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAATATTAGTTGTGCTGAACGAAAAGAGAACAACGATCCTTCGTTCACAGTGGGGTAGTTTTCGGATACATGACGAGGAGCTGCACGATGACTGAACAGGATACAACACCCGATGACCTGGCTCTCACAAGGACGAATGGCGAGCAGGAGAAGAAAACTATTACTGCAGaaacg                         >  2:83887/1‑248 (MQ=255)                                                                                                                                                                                                                     atT‑GTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCACCAACAACCGATGAACTGGCTTTCACAAGGCCGTCTGGCGAGCAGGAGAAGCACATTCTTCCTGCCGAAGCGGt                       >  2:137181/1‑248 (MQ=255)                                                                                                                                                                                                                           tATCAACAAGTTATCAAGTCTTTATAATTAAAATGGTAATTGTTTTTGATTTTGCATTTTAATTGAGAAGTCTTAGTTGTGCTGAACGAAATGAGCACAACGATCCTTCGTTCACAGTGGGGCAGTTTTAGGATCCATGACGCGGAGCTGCACGATGACTGAACAGGCAAAAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGACGCAATTTCTTCCTTCCGAAGCGGTAGAATTTc               >  2:267367/1‑251 (MQ=255)                                                                                                                                                                                                                           tATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTGAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAAtgt                <  2:237093/250‑3 (MQ=255)                                                                                                                                                                                                                                   aGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAATTGAGTAGTATTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGTAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAACAGAAAAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTctgactg         >  2:174418/1‑249 (MQ=255)                                                                                                                                                                                                                                        tCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGt  >  1:351530/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATT‑GTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGT  >  NC_000913/4215107‑4215587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑